TY - JOUR ID - 9464 TI - Extract of mouse embryonic stem cells induces the expression of pluripotency genes in human adipose tissue-derived stem cells JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Motamen Salehi, Paria AU - Foroutan, Tahereh AU - Javeri, Arash AU - Taha, Masoumeh Fakhr AD - Department of Stem Cells and Regenerative Medicine, Institute for Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran AD - Department of Biology, Faculty of Basic Science, Kharazmi University, Tehran, Iran Y1 - 2017 PY - 2017 VL - 20 IS - 11 SP - 1200 EP - 1206 KW - Adipose tissue-derived stem cells KW - Dedifferentiation KW - Embryonic stem cells extract KW - Reprogramming DO - 10.22038/ijbms.2017.9464 N2 - Objective(s): In some previous studies, the extract of embryonic carcinoma cells (ECCs) and embryonic stem cells (ESCs) have been used to reprogram somatic cells to more dedifferentiated state. The aim of this study was to investigate the effect of mouse ESCs extract on the expression of some pluripotency markers in human adipose tissue-derived stem cells (ADSCs). Materials and Methods: Human ADSCs were isolated from subcutaneous abdominal adipose tissue and characterized by flow cytometric analysis for the expression of some mesenchymal stem cell markers and adipogenic and osteogenic differentiation. Frequent freeze-thaw technique was used to prepare cytoplasmic extract of ESCs. Plasma membranes of the ADSCs were reversibly permeabilized by streptolysin-O (SLO). Then the permeabilized ADSCs were incubated with the ESC extract and cultured in resealing medium.After reprogramming, the expression of some pluripotency genes was evaluated by RT-PCR and quantitative real-time PCR (qPCR) analyses. Results: Third-passaged ADSCs showed a fibroblast-like morphology and expressed mesenchymal stem cell markers. They also showed adipogenic and osteogenic differentiation potential. QPCR analysis revealed a significant upregulation in the expression of some pluripotency genes including OCT4, SOX2, NANOG, REX1 and ESG1 in the reprogrammed ADSCs compared to the control group. Conclusion: These findings showed that mouse ESC extract can be used to induce reprogramming of human ADSCs. In fact, this method is applicable for reprogramming of human adult stem cells to a more pluripotent sate and may have a potential in regenerative medicine. UR - https://ijbms.mums.ac.ir/article_9464.html L1 - https://ijbms.mums.ac.ir/article_9464_0543a3b60ce34f0c12847129160dee1e.pdf ER -