TY - JOUR ID - 9540 TI - The role of TSG-6 and uroplakin III in bladder pain syndrome/ interstitial cystitis in rats and humans JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Lv, Yi-Song AU - Gao, Rui AU - Lin, Qing-Ming AU - Jiang, Tao AU - Chen, Qin AU - Tang, Song-Xi AU - Mao, Hou-Ping AU - Zhou, Hui-Liang AU - Cao, Lin-Sheng AD - Department of Urology, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, China AD - Department of Emergency Medicine, Fujian Provincial Hospital, Fuzhou 350005, China Y1 - 2017 PY - 2017 VL - 20 IS - 11 SP - 1242 EP - 1249 KW - Bladder pain syndrome/interstitial cystitis KW - Immunofluorescence staining KW - Interleukin-6 KW - TSG-6 KW - Uroplakin III DO - 10.22038/ijbms.2017.9540 N2 - Objective(s):We investigated the relationship between the expression of tumor necrosis factor-inducible gene 6 (TSG-6) with inflammation and integrity of the bladder epithelium in the bladder tissues of patients with bladder pain syndrome/interstitial cystitis (BPS/IC) and the mechanism of action using a rat model of BPS/IC.   Materials and Methods: Expression of TSG-6 and uroplakin III was determined by immuno- histochemistry of bladder biopsy samples from control human subjects and patients with verified BPS/IC. Our rat model of BPS/IC was employed to measure the perfusion of bladders with hyaluronidase, and assessment of the effect of TSG-6 administration on disease progression. Treatment effects were assessed by measurement of metabolic characteristics, RT-PCR of TGR-6 and interleukin-6, bladder histomorphology, and immunohistochemistry of TGR-6 and uroplakin III. Results: The bladders of patients with BPS/IC had lower expression of uroplakin III and higher expression of TSG-6 than controls. Rats treated with hyaluronidase for 1 week developed the typical signs and symptoms of BPS/IC, and rats treated with hyaluronidase for 4 weeks had more serious disease. Administration of TSG-6 reversed the effects of hyaluronidase and protected against disease progression. Conclusion:Our results indicate that TSG-6 plays an important role in maintaining the integrity of the bladder epithelial barrier. UR - https://ijbms.mums.ac.ir/article_9540.html L1 - https://ijbms.mums.ac.ir/article_9540_c61dca2e074f66620074a870e00e5204.pdf ER -