TY - JOUR ID - 9622 TI - The effects of activated-omental extract on nuclear and cytoplasmic in vitro maturation of rat oocytes JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Mesbah, Fakhroddin AU - Donic Pracha, Aris AU - Talaei-Khozani, Tahereh AU - Bahmanpour, Soghra AD - Department of Anatomical Sciences, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Y1 - 2017 PY - 2017 VL - 20 IS - 12 SP - 1345 EP - 1353 KW - Cumulus cells KW - Cytoplasm KW - In vitro oocyte maturation KW - Nucleus KW - Omentum KW - Rats DO - 10.22038/ijbms.2017.9622 N2 - Objective: The role of growth factors, including vascular endothelial growth factor of activated omentum on mitosis is clearly known, though not on all the aspects of in vitro oocyte maturation. This study was designed to assess the effect of activated-omental extract (AOE) on in vitro maturation (IVM) of rat cumulus-oocyte complexes (COCs). Materials and Methods: In this experimental study, the COCs were incubated in Ham’s F-10 supplemented with either 20% AOE, 20% fetal bovine serum (FBS) or serum-free media. Post-culture COCs were studied according to the cumulus cells (CCs) expansion, nuclear maturation and cytoplasmic maturation. Cumuli expansion was evaluated by inverted microscope without staining; nuclear maturation was assessed by aceto-orcein staining (light microscope) and cytoplasmic maturation was also observed by TEM. Results: Expansion of CCs and nuclear maturation of the oocytes in in vitro for 24 hr was significantly higher in AOE- and FBS-supplemented groups (P=0.000 and 0.013) and (P=0.004 and 0.014), respectively, compared to serum-free group. At ultra-structural level, after 24 hr, both FBS and AOE-supplemented media showed uniformly wide perivitelline space (PVS). After 12 hr, the cortical granules were found in the oocytes cultured in FBS and AOE-supplemented media. Within 24 hr, both granules and mitochondria were large without any detectable topographic tendency across the ooplasm. In AOE and FBS- supplemented oocytes, the number and size of microvilli were more than those in serum-free one. Conclusion: Although AOE supplementation induced a higher rate of the CCs expansion, and resuming meiosis, it was not as potent as FBS to provide cytoplasmic maturation of rat oocytes. UR - https://ijbms.mums.ac.ir/article_9622.html L1 - https://ijbms.mums.ac.ir/article_9622_3e1d88e673ae69364e54306b69fc5162.pdf ER -