2024-03-29T00:18:50Z
https://ijbms.mums.ac.ir/?_action=export&rf=summon&issue=475
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3866
2014
17
6
Production and characterization of polyclonal antibody against a synthetic peptide from β-actin protein
Nazila
Amini
Mohadeseh
Naghi Vishteh
Omid
Zarei
Reza
Hadavi
Negah
Ahmadvand
Hodjattallah
Rabbani
Mahmood
Jeddi-Tehrani
Objective(s):Antibodies against actin, as one of the most widely studied structural and multifunctional housekeeping proteins in eukaryotic cells, are used as internal loading controls in western blot analyses. The aim of this study was to produce polyclonal antibody against a synthetic peptide derived from N-terminal region of β-actin protein to be used as a protein loading control in western blot and other assay systems. Materials and Methods: A synthetic peptide derived from β-actin protein was designed and conjugated to Keyhole limpet hemocyanin (KLH (and used to immunize a white New Zealand rabbit. The antibody was purified from serum by affinity chromatography column. The purity of the antibody was determined by SDS-PAGE and its ability to recognize the immunizing peptide was measured by ELISA. The reactivity of the antibody with β-actin protein in a panel of different cell lysates was then evaluated by western blot. In addition, the reactivity of the antibody with the corresponding protein was also evaluated by Immunocytochemistry and Immunohistochemistry in different samples. Results: The antibody could recognize the immunizing peptide in ELISA. It could also recognize β-actin protein in western blot as well as in immunocytochemistry and immunohistochemistry. Conclusion: Our data suggest that this antibody may be used as an internal control in western blot analyses as well as in other immunological applications such as ELISA,immunocytochemistry and immunohistochemistry.
antibody
β-actin Immunocytochemistry
Immunohistochemistry Peptide
Western blot
2014
06
01
396
400
https://ijbms.mums.ac.ir/article_2922_9b8238e0d5e29ca4a3e62f744be2bbde.pdf
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3866
2014
17
6
Comparison of the genetic convergence between myco-bacterium strains by three RFLP-based methods in central province of Iran
Aliasghar
Farazi
Mansoureh
Jabbariasl
Keyvan
Tadayon
Nader
Mossavar
Roohollah
Keshavarz
Seyed davood
Hoseini
Nilüfer
Orhan
Didem
Deliorman Orhan
Mustafa
Aslan
Fatma
Ergun
Objective(s):The utilization of molecular techniques in the epidemiology of tuberculosis have provided an opportunity for using effective markers to trace the transmission of the disease .The purpose of this study was to compare the genetic patterns of Mycobacterium tuberculosis by three methods of RFLP technique. Materials and Methods: In a cross-sectional and prospective study, 95 strains of M. tuberculosis isolates were selected for DNA fingerprinting. Extraction of DNA from Mycobacterium strains and DNA fingerprinting with IS-6110, PGRS and DR probe were performed by standard protocols. Results:Overall, the diversity of RFLP among 95 tuberculosis patients were 48, 50 and 45 on the basis of IS6110, PGRS and DR patterns, respectively. Twenty of these patterns (21.1%) with IS6110-RFLP, twenty-two (23.2%) with PGRS-RFLP and seventeen (17.9%) with DR-RFLP occurred with unique RFLP patterns, whereas the remaining 28 patterns were communal. The risk factors of clustering among tuberculosis patients were age < 45 years, new cases, degree of sputum smear ≥ 2+, and close contact. Conclusion: Our study demonstrated that IS6110-RFLP, PGRS-RFLP and DR-RFLP genotyping could roughly identify similar proportions of clustered (secondary) cases as well as the same risk factors for clustering.
DR-RFLP
Genetic convergence
IS6110-RFLP
Mycobacterium tuberculosis PGRS-RFLP
2014
06
01
401
405
https://ijbms.mums.ac.ir/article_2923_fabeb0ac5fe17e1f1a91d07489d88491.pdf
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3866
2014
17
6
The effects of cyclooxygenase inhibitors on the gastric emptying and small intestine transit in the male rats following traumatic brain injury
Zakieh
Keshavarzi
Mohammad
Khaksari
Nader
Shahrokhi
Objective(s):This study was carried out to investigate the effects of COX-2 selective inhibitor (Celecoxib) or non-selective COX inhibitor (Ibuprofen) on gastrointestinal motility. Materials and Methods: The rats were randomly divided into five groups including: intact, sham, traumatic brain injury (TBI) group (intact rats under TBI), Celecoxib group (10 mg/kg), Ibuprofen group (10 mg/kg). Rats of the treatment groups received gavages at 1 hr before the TBI induction. The TBI was moderate and diffused using the Marmarou method. The gastric emptying and small intestine transit were measured by phenol red method. Results: The gastric emptying didn’t change following TBI induction compared to intact group. The consumption of ibuprofen or celecoxib didn’t have any effect on gastric emptying compared to sham group. TBI induction didn’t have any effect on the intestinal transit. Also, there was no significant difference between ibuprofen or celecoxib consumption vs. sham group (P>0.05). Conclusion: The COX-2 selective inhibitor (celecoxib) or non-selective COX inhibitor (ibuprofen) have no effects on gastric or small bowel transit. Further work is necessary to investigate the effects of non-selective COX inhibitors and their impact on gastrointestinal motility disorders.
Brain injury
Celecoxib
Gastric Emptying
Ibuprofen
2014
06
01
406
410
https://ijbms.mums.ac.ir/article_2924_16dbd637afec3c3239860a395de2a9c2.pdf
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3866
2014
17
6
The effects of nano-silver and garlic administration during pregnancy on neuron apoptosis in rat offspring hippocampus
Maryam
Lale Ataei
Ali Reza
Ebrahimzadeh-bideskan
Objective(s):The aim of this study was to investigate the effects of nano-silver and garlic administration during pregnancy on neuron apoptosis in rat offspring hippocampus. Materials and Methods: Fifty pregnant wistar rats were randomly divided into five groups: 1- nano- silver (N.S) group; 30 mg/kg of N.S treated via gavage. 2- Control (C) group, administrated with distilled water via gavage. 3- N.S and garlic (N.S+G) group; N.S (30 mg/kg) and garlic juice (1 ml/100 g) treated via gavage simultaneously. 4- Garlic group (G); garlic juice (1 ml/100 g) administrated via gavage, 5- normal (N) without any intervention. All the interventions were done during pregnancy (21 days). Finally, the brains of rat offspring were removed to use for nano-silver level measurement and TUNEL staining. The mean of TUNEL positive cell numbers per unit area (NA) in different regions of hippocampus were compared in all animal groups. Results: The results revealed a significant increase of hippocampus nano-silver level in N.S and N.S+G groups comparing to N group (P<0.05) and a significant decrease in nano-silver level in N.S+G group comparing to N.S group (P<0.01). The number of TUNEL positive cells in the CA1, CA3, and DG fields of rat offspring hippocampus increased in N.S and N.S+G groups comparing to other ones, and also reduced significantly in N.S+G group comparing to N.S group (¥ P< 0.01). Conclusion: Our results showed that co-administration of nano-silver and garlic during pregnancy may lead to reduce nano-silver induced apoptotic cells in their offspring hippocampus.
Apoptosis
Garlic
Hippocampus
Nano-silver
2014
06
01
411
418
https://ijbms.mums.ac.ir/article_2925_14e0bf3eb79fedb7fb4c97a1b556df18.pdf
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3866
2014
17
6
The effects of tramadol on norepinephrine and MHPG releasing in locus coeruleus in formalin test in rats: a brain stereotaxic study
Mohammad Ali
Mobasher
Javad
Sajedianfard
Akram
Jamshidzadeh
Naser
Naghdi
Mohammad Mehdi
Namvaran
Objective(s):The relationship between tramadol, as an antinociceptive drug, and locus coeruleus (LC), the main noradrenergic nucleus of the brain that affects regulation and modulation of pain through descending noradrenergic pathways was investigated. Materials and Methods: Male Sprague-Dawley rats were divided into four groups of 10 rats. The rats were fixed in stereotaxic instrument and then a probe was inserted into LC. Pain was induced by subcutaneous injection of 50 μl of 2.5% formalin 40 minutes after initiation of microdialysisin right hind paw, and nociceptivepain scores were calculated every 5 minutes. Subsequently noradrenaline (NA) and its metabolite, 3-methoxy-4-hydroxyphenylglycol (MHPG), were collected and measured by microdialysis of locus coeruleus in freely moving rats every 15 minutes during formalin injection. Results: Nociceptive pain scores observed in formalin test had the highest nociceptive sensation 5 minutes after injection. Significant rises in concentrations of NA and MHPG, in samples taken between 30 and 45 min after initiation of the locus coeruleusmicrodialysis, coincided with the peak of the pain after injection of formalin. Conclusion: According toconcurrency of the highest nociceptive sensation and peak of NE and MHPG concentrations, tramadol can indirectly affect the LC by blocking the pain signals from different parts of the brain such as periaqueductal gray mater, central nucleus of amygdale or the spinal cord.
Antinociceptive
Locus coeruleus
Microdialysis
Stereotaxic instrument
Tramadol
2014
06
01
419
425
https://ijbms.mums.ac.ir/article_2926_9105b316871d19fcb527245b2cbc7093.pdf
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3866
2014
17
6
Enzyme inhibitory and radical scavenging effects of some antidiabetic plants of Turkey
Nilüfer
Orhan
Sanem
Hoşbaş
Didem
Deliorman Orhan
Mustafa
Aslan
Fatma
Ergun
Objective(s):Ethnopharmacological field surveys demonstrated that many plants, such as Gentiana olivieri, Helichrysum graveolens, Helichrysum plicatum ssp. plicatum, Juniperus oxycedrus ssp. oxycedrus, Juniperus communis var. saxatilis, Viscum album (ssp. album, ssp. austriacum), are used as traditional medicine for diabetes in different regions of Anatolia. The present study was designed to evaluate the in vitro antidiabetic effects of some selected plants, tested in animal models recently. Materials and Methods: α-glucosidase and α-amylase enzyme inhibitory effects of the plant extracts were investigated and Acarbose was used as a reference drug. Additionally, radical scavenging capacities were determined using 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) ABTS radical cation scavenging assay and total phenolic content of the extracts were evaluated using Folin Ciocalteu method. Results: H. graveolens ethanol extract exhibited the highest inhibitory activity (55.7 % ± 2.2) on α-amylase enzyme. Additionally, J. oxycedrus hydro-alcoholic leaf extract had potent α-amylase inhibitory effect, while the hydro-alcoholic extract of J. communis fruit showed the highest α-glucosidase inhibitory activity (IC50: 4.4 μg/ml). Conclusion:Results indicated that, antidiabetic effect of hydro-alcoholic extracts of H. graveolens capitulums, J. communis fruit and J. oxycedrus leaf might arise from inhibition of digestive enzymes.
α-Amylase
α-Glucosidase
Gentiana olivier
Helichrysum
Juniperus
Viscum album
2014
06
01
426
432
https://ijbms.mums.ac.ir/article_2927_7371e14044edda934718ce3fe3e94c44.pdf
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3866
2014
17
6
Gender difference in motor impairments induced by chronic administration of vinblastine
Shahrnaz
Parsania
Mohammad
Shabani
Kasra
Moazzami
Moazamehosadat
Razavinasab
Mohammad Hassan
Larizadeh
Masoud
Nazeri
Majid
Asadi-Shekaari
Moein
Kermani
Objective(s):Neurotoxicity of anticancer drugs complicates treatment of cancer patients. Vinblastine (VBL) is reported to induce motor and cognitive impairments in patients receiving chronic low-dose regimen. Materials and Methods: The effects of VBL treatment on motor, learning and memory functions of male and female Wistar rats were studied by behavioral related tests. Animals were given chronic intraperitoneal injections of VBL (0.2 mg/kg/week for 5 weeks) from postnatal day 23 to 52. Motor function was evaluated using grasping test and balancing was evaluated by the rotarod. Spatial learning and memory and anxiety-like behavior were determined using Morris water maze (MWM) task and open field test, respectively. Results: Administration of VBL caused severe damage to motor and balance function of male rats in comparison to female rats treated with VBL and rats treated with saline. Memory and locomotion were affected in both male and female rats compared with saline treated rats, while a sex difference was also observed in these parameters; male rats showed more impairment compared with female ones. Both male and female rats showed cognitive impairments in MWM task and no sex differences were observed in these functions. Conclusion: Results revealed that VBL is a potent neurotoxic agent and despite the profound effect of VBL on motor and cognitive functions, it seems that male rats are more susceptible to motor deficits induced by VBL.
Anticancer
Learning and memory
motor function
Vinblastine
2014
06
01
433
440
https://ijbms.mums.ac.ir/article_2928_16ee86a3671ca0857a86cb6c53c4e652.pdf
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3866
2014
17
6
Frequencies of two functionally significant SNPs and their haplotypes of organic anion transporting polypeptide 1B1 SLCO1B1 gene in six ethnic groups of Pakistani population
Tausif Ahmed
Rajput
Abdul Khaliq
Naveed
Shakir
Khan
Zia-Ur
Rehman Farooqi
Objective(s):Organic anion transporter polypeptide 1B1 (OATP1B1) encoded by solute carrier organic transporter 1B1 (SLCO1B1) gene; a transporter involved in the uptake of drugs and endogenous compounds is present in hepatocyte sinusoidal membrane. Aim of this study was to investigate the frequencies of functionally significant SNPs (388A>G and 521T>C) and their haplotypes in 6 ethnic groups of Pakistani population through the development of rapid and efficient Tetra amplification refractory mutation system (T. ARMS) genotyping assay. Materials and Methods: Frequencies of alleles, genotype, and haplotypes of two functionally significant Single nucleotide polymorphism in 180 healthy Pakistani subjects and distributions in six ethnic groups by using a single step T. ARMS genotyping assay. Results: The allelic frequency for 388A>G SNP was 50% in total Pakistani population with distributions of 9.7%, 15.1%, 19.4%, 16.1%, 18.3%, and 21.5% in Punjabi, Sindhi, Balouchi, Pathan, Kashmiri and Hazara/Baltistan groups respectively; and for 521T>C SNP it was 23.9% in total Pakistani population with distributions of 11.1%, 8.9%, 15.6%, 11.1%, 31.1% and 22.2% in Punjabi, Sindhi, Balouchi, Pathan, Kashmiri, and Hazara/Baltistan groups. Both functionally significant SNPs occurred in four major haplotypes with a frequency of 35.5% for 388A/521T (*1A), 40.5% for 388G/521T (*1B), 14.4% for 388A/521C (*5), and 9.4% for 388G/521C (*15) with varying distributions among six ethnic groups. Conclusion: The 388A>G and 521T>C genotypes and corresponding haplotypes are present at varying frequencies in various ethnic groups of Pakistani population. Pharmacokinetic and pharmacodynamic
Haplotype
OATP1B1
single nucleotide polymorphism
SLCO1B1
2014
06
01
441
447
https://ijbms.mums.ac.ir/article_2929_abeca501d9b5d89c21ffc66a9cc6fcfc.pdf
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3866
2014
17
6
Pantoprazole, a proton pump inhibitor, increases orthodontic tooth movement in rats
Mohsen
Shirazi
Houman
Alimoradi
Yasaman
Kheirandish
Shahroo
Etemad‐Moghadam
Mojgan
Alaeddini
Alipasha
Meysamie
Seyed Amir Reza
Fatahi Meybodi
Ahmad Reza
Dehpour
Objective(s):Pantoprazole, is a proton pump inhibitor (PPI) prescribed for the treatment of upper gastrointestinal disorders, which in high doses has been suggested to decrease calcium absorption leading to hypocalcaemia and therefore osteoporosis. The aim of this study was to assess whether pantoprazol, could alter the rate of orthodontic tooth movement (OTM) in rats. Materials and Methods: A time course study was established using 72 rats which were divided into six groups of 12 samples each (four: vehicle; eight: pantoprazole + vehicle). Pantoprazole at a dose of 200 mg/kg suspended in carboxymethyl cellulose (0.25 percent) was administered by a gastric tube. The upper incisors and first molars were ligated by a 5 mm nickel-titanium closed-coil spring to deliver an initial force of 60 g. Animals were euthanized two weeks after orthodontic treatment followed by assessment of tooth movement and histomorphometric evaluation of the detached maxillae. Lateral skull radiographs were obtained once a week, starting from the first day to the 6th week of the study. OTM and bone density data were analyzed using independent sample t-test and repeated measures ANOVA. Results: No significant changes in OTM measurements and optical density were observed in vehicle-receiving animals during the study (P=0.994). OTM was significantly increased after six weeks pantoprazole therapy which continued until the 7th week of the experiment (P=0.007). Optical density significantly increased in the pantoprazole-treated rats after six weeks. Conclusion: Long term PPI therapy at high doses could lead to osteoporosis and enhanced OTM.
Pantoprazole
Proton pump inhibitors
Radiography
Rats
Tooth movement
2014
06
01
448
453
https://ijbms.mums.ac.ir/article_2930_9e94db700a477e5242e1cd3e22507324.pdf
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3866
2014
17
6
Mild hypothermia reduces expression of Fas/FasL and MMP-3 after cerebral ischemia-reperfusion in rats
Jingkun
Zhao
Shurong
Duan
Jinxia
Zhou
Ruihong
Sun
Liming
Zhang
Desheng
Wang
Objective(s):To investigate the effects of local mild hypothermia on the expression of Fas, FasL and MMP-3 after cerebral ischemia-reperfusion in rats. Materials and Methods:Male Wistar rats were divided into sham-operated group (Sham), normothermia group (NT), and hypothermia group (HT). MCAO/R model was established by Longa’s method, and reperfusion was allowed after 2 hr occlusion. Mild hypothermia (33±0.5℃) for 6 hr was initiated at the start of reperfusion. Immunohistochemistry was performed to determine expression Fas, FasL, and MMP-3. Results: Infarct volume was reduced in the hypothermia group (18.43±4.23%) compared with the normothermia group (24.76±5.76%) (P<0.05). In mild hypothermia group, numbers of Fas-positive and MMP-3 positive cells were significantly less than those of normothermia group (P<0.05). Neurological functional scores of mild hypothermia were significantly improved (P<0.05). Conclusion: Mild hypothermia decreases infarct volume after cerebral ischemia-reperfusion, reduces Fas and MMP-3 expression, but increases FasL in cerebral ischemia-reperfusion rats.
Cerebral ischemia/reperfusion
Fas
FasL
Hypothermia
MACO rat
MMP-3
2014
06
01
454
459
https://ijbms.mums.ac.ir/article_2931_b923cc1f5f757de29207887f9de1a18c.pdf
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3866
2014
17
6
Antioxidant effects of proanthocyanidin from grape seed on hepatic tissue injury in diabetic rats
Esrafil
Mansouri
Layasadat
Khorsandi
Hassan Ali
Abedi
Objective(s):Diabetes plays an important role in the induction of the liver injury. Grape seed proanthocyanidin (GSP) have a wide range of medicinal properties against oxidative stress. In this study we evaluated antioxidant effects of GSP on liver in streptozotocin-induced diabetic rats. Materials and Methods: Thirty male Sprague–Dawley rats were divided into three groups: control, untreated diabetic and diabetic rats treated with GSP. Diabetes was induced in rats by intraperitoneal injection of streptozotocin (50 mg/kg). GSP were administered via oral gavage (200 mg/kg) for 4 weeks. Results: GSP produced significant hepatoprotective effects by decreasing activities of serum aminotransferases and alkaline phosphatase, and decreasing liver malondialdehyde and bilirubin (P<0.05) levels. It increased liver superoxide dismutase, catalase and glutathione peroxidase activities and albumin level (P<0.05). Administration of GSP significantly ameliorated structural changes induced in liver of diabetic rats. Conclusion: GSP have protective effects against hepatic tissue injury due to antioxidant properties.
Antioxidant enzyme
Diabetes
GSP
Hepatic tissue
2014
06
01
460
464
https://ijbms.mums.ac.ir/article_2932_4b370f73091baabac68fd27b43411d73.pdf
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3866
2014
17
6
Evaluation of alpha- amylase inhibition by Urtica dioica and Juglans regia extracts
Mahsa
Rahimzadeh
Samaneh
Jahanshahi
Soheila
Moein
Mahmood Reza
Moein
Objective(s):One strategy for the treatment of diabetes is inhibition of pancreatic α- amylase. Plants contains different chemical constituents with potential for inhibition of α-amylase and hence maybe used as therapeutic. Materials and Methods: Urtica dioica and Juglans regia Linn were tested for α-amylase inhibition. Different concentrations of leaf aqueous extracts were incubated with enzyme substrate solution and the activity of enzyme was measured. For determination of the type of inhibition, Dixon plot was depicted. Acarbose was used as the standard inhibitor. Results: Both plant extracts showed time and concentration dependent inhibition of α-amylase. 60% inhibition was seen with 2 mg/ml of U. dioica and0.4 mg/ml of J. regia aqueous extract. Dixon plots revealed the type of α-amylase inhibition by these two extracts as competitive inhibition. Conclusion: Determination of the type of α-amylase inhibition by these plant extracts could provide by successful use of plant chemicals as drug targets.
Amylase inhibition
Diabetes
Plant extract
2014
06
01
465
469
https://ijbms.mums.ac.ir/article_2933_a2a6170dfb40c196c4d864c01c7ebd59.pdf