Mangosteen peel extract (Garcinia mangostana L.) as protective agent in glucose-induced mesangial cell as in vitro model of diabetic glomerulosclerosis

Document Type : Short Communication

Authors

1 Faculty of Medicine, Maranatha Christian University, Bandung 40164, West Java, Indonesia

2 Faculty of Pharmacy, Pancasila University, Jakarta Selatan 12630, DKI Jakarta, Indonesia

3 Aretha Medika Utama, Biomolecular and Biomedical Research Center, Bandung 40163, West Java, Indonesia

4 School of Pharmacy Bandung Insitute of Technology, Bandung 40132, West Java, Indonesia

Abstract

Objective(s): This study aims to evaluate the activity of mangosteen peels extract (MPE) as protection agent on induced-glucose mesangial cells (SV40 MES 13 cell line (Glomerular Mesangial Kidney, Mus Musculus)).  
Materials and Methods: MPE was performed based on maceration method. Cytotoxic assay was performed based on MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) method, while the level of TGF-β1 (Transforming growth factor-β1) and fibronectin in glucose-induced mesangial cells were assayed and determined using ELISA KIT.
Results: In viability assay, MPE 5 and 20 µg/ml has the highest activity to increase cells proliferation in glucose-induced mesangial cells at 5, 10, and 15 days of incubation in glucose concentration (5 and 25 mM) (P<0.05). In inhibitory activity of TGF-β1 and fibronectin level, MPE 5 µg/ml (glucose-induced 5 mM) show the lowest level compared to positive control and other treatments (P<0.05).
Conclusion: MPE can increase cell proliferation in glucose-induced mesangial cells and significantly reduce the level of TGF-β1 and fibronectin. MPE activity has correlates to inhibit the diabetic glomerulosclerosis condition and may increase mesangial cell proliferation.

Keywords

Main Subjects

References

1. Shaw JE, Sicree RA, Zimmet PZ. Global estimates of the prevelence of diabetes for 2010 and 2030. Diabetes Res Clin Pract 2010; 87:4-14.
2. Kashihara N, Haruna Y, Kondeti VK, Kanwar YS. Oxidative stress in diabetic nephropathy. Curr Med Chem 2013; 17:4256-4269.
3. Giacco F, Brownlee M. Oxidative stress and diabetic complications. Circ Res 2010; 107:1058-1070.
4. Liu Y, Lu S, Zhang Y, Wang X, Kong F, Liu Y. Role of caveolae in high glucose and TGF-β1 induced fibronectin production in rat mesangial cells. Int J Clin Exp Pathol 2014; 7:8381-8390.
5. Qian Y, Feldman E, Pennathur S, Kretzler M, Brosius FC. From fibrosis to sclerosis mechanisms of glomerulosclerosis in diabetic nephropathy. Diabetes 2008; 57:1439-1445.
6. Alsaad KO, Herzenberg AM. Distingusihing diabetic nephropathy from other causes of glomerulosclerosis: an update. J Clin Pathol 2007; 60:18-26.
7. Zhu Y, Usui HK, Sharma K. Regulation of transforming growth factor-beta in diabetic nephropathy: implications for treatment. Sem Nephrol 2007; 27:153-160.
8. Patil SB, Patil GS, Kundaragi VS, Biradar AN. A case of xanthogranulomatous pyelonephritis with spontaneous renocolic fistula. Turk J Urol 2013; 39:122-125.
9. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional food: impact on human health. Pharmacog 2010; 4:118-126.
10. Chong YM, Chang SK, Sia WCM, Yim HS. Antioxidant efficacy of mangosteen (Garcinia mangostana Linn.) peel extracts in sunflower oil during accelerated storage. Food Biosci. 2015; 12:18-25.
11. Pedraza-Chacerri J, Cardenas-Rodriguez N, Orozco-Ibarra M, Perez-Rojaz JM. Medicinal properties of mangosteen (Garcinia mangostana). Food Chem Toxicol 2008; 46:3227-3239.
12. Tewtrakul S, Wattanapiromsakul C, Mahabusarakam W. Effects of compounds from Garcinia mangostana on inflammatory mediators in RAW 264.7 macrophage cells. J Ethnopharm 2009; 121:379-382.
13. Widowati W, Darsono L, Suherman J, Fauziah N, Maesaroh M. Anti-inflammatory effect of mangosteen (Garcinia mangostana L.) peel extract and its compounds in LPS-induced RAW264.7 Cells. Cells Nat Prod Sci 2016; 22:147-153.
14. Novilla A, Djamhuri DS, Fauziah N, Maesaroh M, Balqis B, Widowati W. Cytotoxic activity of mangosteen (Garcinia mangostana L.) peel extract and α-mangostin toward leukemia cell lines (HL-60 and K-562). J Nat Remed 2016; 16:52-59.
15. Zadernowski R, Czaplicki S, Naczk M. Phenolic acid profiles of mangosteen fruits (Garcinia mangostana). Food Chem 2009; 112:153-160.
16. Pothitirat W, Chomnawang MT, Supabphol R, Gritsanapan W. Comparison of 543 bioactive compounds content, free radical scavenging and anti-acne inducing bacteria 544 activities of extracts from the mangosteen fruit rind at two stages of 545 maturity. Fitoter 2009; 80:422-447.
17. Suttirak W, Manurakchinakom S. In vitro antioxidant properties of mangosteen peel extract. J Food Sci Tech 2012; 51:3546-3558.
18. Widowati W, Rusmana D, Hardiman H, Tiono H, Wargasetia TL, Pujimulyani D. Mangosteen peel (Garcinia mangostana L.) extract for effervescent tablet. Proceeding World Academy of Sci, Eng & Tech 2013; 82:190-195.
19. Widowati W, Darsono L, Suherman J, Yelliantty Y, Maesaroh M. High Performance Liquid Chromatography (HPLC) analysis, antioxidant, antiaggregation of mangosteen peel extract (Garcinia mangostana L.). Int J Biosci Biochem Bioinform 2014; 4:458-466.
20. Adnyana IK, Abuzaid AS, Iskandar EY, Kurniati NF. Pancreatic lipase and α-amylase inhibitory potential of mangosteen (Garcinia mangostana Linn.) pericarp extract. Int J Med Res Health Sci 2016; 5:23-28.
21. Abuzaid AS, Iskandar EY, Kurniati NF, Adnyana IK. Prevention of obesity and development of metabolic syndrome by mangosteen (Garcinia mangostana L.) pericarp ethanolic extract in male wistar rats fed with high-fat diet. Int J Pharm Pharmaceutic Sci 2016. 2016; 8:1-7.
22. Abuzaid AS, Iskandar EY, Kurniati NF, Adanyana IK. Preventive effect on obesity of mangosteen (Garcinia mangostana L.) pericarp ethanolic extract by reduction of fatty acid synthase level in monosodium glutamate and high-calorie diet-induced male wistar rats. Asian J Pharm Clin Res 2016; 9:257-260.
23. Widowati W, Darsono L, Suherman J, Fauziah N, Maesaroh M, Erawijantari PP. Anti-inflammatory effect of mangosteen (Garcinia mangostana L.) peel extract and its compounds in LPS-induced RAW264.7 cells. Nat Prod Sci 2016; 22: 147-153.
24. Rusmana D, Elisabeth M, Widowati W, Fauziah N, Maesaroh M. Inhibition of inflammatory agent production by ethanol extract and eugenol of Syzigium aromaticum flower bud (clove) in LPS-stimulated RAW264.7 cells. Res J Med Plants 2015; 9:264-274.
25. Nahman NS, Leonhart KL, Cosio FG, Hebert CL. Effects of high glucose on cellular proliferation and fibronectin production by cultured human mesangial cells. Kidney Int 1992; 41:396-402.
26. Dai MM, Wu H, Li H, Chen J, Chen JY, Hu SL, et al. Effects and mechanisms of Geniposide on rats with adjuvant arthritis. Int Immunopharmacol 2014; 20:46-53.
27. Pankov R, Yamada KM. Non-radioactive quantification of fibronectin matrix assembly. Curr Protoc Cell Biol 2004; 10:10-13.
28. Kolset SO, Reinbolt FP, Jenssen T. Diabetic nephropathy and extracelullar matrix. J Histochem Cytochem 2012; 60:976-986.
29. Qian Y, Wang X, Liu Y, Li Y, Colvin RA, Tong L, et al. Extracellular ATP is internalized by macropinocytosis and induces intracellular ATP increase and drug resistance in cancer cells. Cancer Lett 2014; 351:242-251.
30. Loeffler I, Wolf G. Transforming growth factor-β and the progression of renal disease. Nephrol Dial Transplant 2013; 29:37-45.
31. Trachtman H, Futterweit S, Prenner J, Hanon S. Antioxidants reverse the antiproliferative effect of high glucose and advanced glycosylation end products in cultured rat mesangial cells. Biochem Biophys Res Commun 1994; 199: 346-352.
32. Chen C, Cheng K, Chang AY, Lin Y, Hseu Y, Wang H. 10-Shogaol, an Antioxidant from Zingiber officinale for skin cell proliferation and migration enhancer. Int J Mol Sci 2012; 13:1762-1777.
33. Kurtz A, Oh S. Age related changes of the extracelullar matrix and stem cell maintenance. Prev Med 2012; 54:S50-S56.
34. Lee HS, Song CY. Oxidized low-density lipoprotein and oxidative stress in the development of glomerulosclerosis. Am J Nephrol 2009; 29:62-70.
35. David MS, Coombes JS, Bennett N, Johnson DW, Gobe GC. Oxidative stress, anti-oxidant therapies and chronic kidney disease. Nephrology 2012; 17:311-321.
36. Jha JC, Banal C, Chow BS, Cooper ME, Jandeleit-Dahm K. Diabetes and Kidney Disease: Role of Oxidative Stress. Antioxid Redox Signal 2016; 25:657-684.
37. Manaharan T, Palanisamy UD, Ming CH. Tropical plant extract as potential antihyperglycemic agents. Molecules 2012; 17:5915-5923.
38. Han H, Cao A, Wang L, Guo H, Zang Y, Li Z. Huangqi decoction ameliorates streptozotocin-induced rat diabetic nephropathy through antioxidant and regulation of the TGF-β /MAPK / PPAR-γ signaling. Cell Physiol Biochem 2017; 42:1934-1944.
39. Zhang L, Pang S, Deng B, Qian L, Chen J, Zou J, et al. High glucose induces renal mesangial cell proliferation and fibronectin expression through JNK/NF- B/NADPH oxidase/ ROS pathway, which is inhibited by resveratrol. Int J Biochem Cell Biol 2012; 44:629-638.
40. Dennis JM, Witting PK. Protective role for antioxidants in acute kidney disease. Nutrients 2017; 9(718):1-25.
41. Kurniawati M, Mahdi C, Aulanni’am A. The effect of juice mangosteen rind (Garcinia mangostana L.) to blood sugar levels and histological of pancreatic rats with the induction of streptozotocin. J Pure App Chem Res 2014; 3:1-6.
Iranian Journal of Basic Medical Sciences
Volume 21, Issue 9
September 2018
Pages 972-977

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