The therapeutic effect of ozonated olive oil plus glucantime on human cutaneous leishmaniasis

Document Type: Original Article


1 Skin Diseases and Leishmaniasis Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran

2 Department of Molecular Medicine, School of Advanced Technologies,Shahrekord University of Medical Sciences, Shahrekord, Iran

3 Department of Biostatistics & Epidemiology, School of Public Health, Isfahan University of Medical Sciences, Isfahan, Iran

4 Department of Laboratory Sciences, School of Paramedical Sciences, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran

5 Health Sciences Research Center, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran

6 Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran


Objective(s): Leishmaniasis is one of the main health problems in developing countries, caused by intracellular protozoan parasites of the Leishmania genus. Although research has been successful in discovering vaccines and anti-parasitic drugs like antimony compounds, their side effects like high toxicity, prolonged regeneration, etc., have raised the replacement importance of natural products with antioxidant and antibacterial properties. It can be said that an appropriate alternative to this is the ozonated olive oil. Ozone by introducing O2 in involved tissues and bloodstream could degrade parasite amastigotes and lead to cleared leishmaniasis infections. So, the present study aimed to evaluate the effect of ozonated olive oil in Iranian leishmaniasis patients compared to glucantime, a choice drug for the treatment of Leishmaniasis.
Materials and Methods: Thirty patients with confirmed leishmaniasis lesions were included and divided into two groups, 15 cases as control and 15 cases as test with lesions of 30–50 mm2 in diameter. The control group received glucantime intralesionally and the test group ozonated olive oil plus glucantime, 2 times daily.
Results: The mean of lesion size was (50.94±33.20) before and (15±14.34) after treatment in control (P<0.00) and (50.88±31.74) before and (9.93±14.18) after treatment in the test group (P<0.00). Moreover, the mean course of therapy was 10.4(±1.84) weeks and 8.93(±2.15) weeks in control and test groups, respectively (P=0.636). Significant differences were reported in lesion size after treatment between the two groups (P<0.00).
Conclusion: Data suggested ozonated olive oil can have synergistic effects with glucantime in the treatment of cutaneous leishmaniasis.


Main Subjects

1. Bates PA. Transmission of Leishmania metacyclic promastigotes by phlebotomine sand flies. Int J Parasitol 2007; 37: 1097-1106.
2. Fata A, Salehi G, Rafatpanah H, Mousavi bazzaz M, Mohaghegh MA, Movahedi A. Identification of Leishmania species by kinetoplast DNA-polymerase chain reaction for the first time in Khaf district, Khorasan-e-Razavi province, Iran. Trop Parasitol 2015; 5: 50-54.
3. Desjeux P. The increase in risk factors for leishmaniasis worldwide. Trans R Soc Trop Med Hyg 2001; 95: 239-243.
4. David CV, Craft N. Cutaneous and mucocutaneous leishmaniasis. Dermatolo Ther 2009; 22: 491-502.
5. Hashemi N, Mohaghegh M, Hashemi M, Azami M, Mortazavidehkordi N, Hashemi C, et al. PCR-RFLP diagnosis and characterization of Leishmania species causing human cutaneous leishmaniasis and evaluation of treatment times with glucantime in these patients. Trop Biomed 2016; 33: 689-696.
6. Mirzaei F, Bafghi AF, Mohaghegh MA, Jaliani HZ, Faridnia R, Kalani H. In vitro anti-leishmanial activity of Satureja hortensis and Artemisia dracunculus extracts on Leishmania major promastigotes. J Parasit Dis 2016; 40: 1571-1574.
7. Pulvertaft RJ, Hoyle GF. Stages in the life-cycle of Leishmania donovani. Trans R Soc Trop Med Hyg 1960; 54: 191-196.
8. Machowetz A, Poulsen HE, Gruendel S, Weimann A, Fito M, Marrugat J, et al. Effect of olive oils on biomarkers of oxidative DNA stress in Northern and Southern Europeans. FASEB J 2007; 21: 45-52.
9. Al-Waili NS. Mixture of honey, beeswax and olive oil inhibits growth of Staphylococcus aureus and Candida albicans. Arch  Med Res 2005; 36: 10-13.
10. Perona JS, Arcemis C, Ruiz-Gutierrez V, Catala A. Effect of dietary high-oleic-acid oils that are rich in antioxidants on microsomal lipid peroxidation in rats. J Agric Food Chem 2005; 53: 730-735.
11. Rodriguez-Rodriguez R, Herrera MD, Perona JS, Ruiz-Gutierrez V. Potential vasorelaxant effects of oleanolic acid and erythrodiol, two triterpenoids contained in ‘orujo’ olive oil, on rat aorta. Br J Nutr 2004; 92: 635-642.
12. Marquez-Martin A, De La Puerta R, Fernandez-Arche A, Ruiz-Gutierrez V, Yaqoob P. Modulation of cytokine secretion by pentacyclic triterpenes from olive pomace oil in human mononuclear cells. Cytokine 2006; 36: 211-217.
13. Fernandez-Arche A, Marquez-Martin A, de la Puerta Vazquez R, Perona JS, Terencio C, Perez-Camino C, et al. Long-chain fatty alcohols from pomace olive oil modulate the release of proinflammatory mediators. J Nutr Biochem 2009; 20: 155-162.
14. Tibbles PM, Edelsberg JS. Hyperbaric-oxygen therapy. N Engl J Med 1996; 334: 1642-1648.
15. Rennecker JL, Mariñas BJ, Owens JH, Rice EW. Inactivation of Cryptosporidium parvum oocysts with ozone. Water Res 1999; 33: 2481-2488.
16. Carvajal G, Branch A, Michel P, Sisson SA, Roser DJ, Drewes JE, et al. Robust evaluation of performance monitoring options for ozone disinfection in water recycling using Bayesian analysis. Water Res 2017; 124: 605-617.
17. Mandhare M, Jagdale D, Gaikwad P, Gandhi P, Kadam V. Miracle of ozone therapy as an alternative medicine. Int J Pharm Chem Biol Sci 2012; 2: 63-71.
18. Elvis AM, Ekta JS. Ozone therapy: A clinical review. J Nat Sci Biol Med 2011; 2: 66-70.
19. Torrentino-Madamet M, Almeras L, Desplans J, Le Priol Y, Belghazi M, Pophillat M, et al. Global response of Plasmodium falciparum to hyperoxia: a combined transcriptomic and proteomic approach. Malar J 2011; 10: 4.
20. Wickramanayake GB, Rubin AJ, Sproul OJ. Inactivation of Giardia lamblia cysts with ozone. Appl Environ Microbiol 1984; 48: 671-672.
21. Casimiro E, Calheiros J, Santos FD, Kovats S. National assessment of human health effects of climate change in Portugal: approach and key findings. Environ Health Perspect 2006; 114: 1950-1956.
22. Valacchi G, Bocci V. Studies on the biological effects of ozone: 10. Release of factors from ozonated human platelets. Mediators Inflamm 1999; 8: 205-209.
23. Bulynin V, Ermakova A, Glukhov A, Mozhurov I. Wound treatment using the flow of an ozonized solution under high pressure. Khirurgiia 1997: 23-24.
24. Gajendrareddy PK, Sen CK, Horan MP, Marucha PT. Hyperbaric oxygen therapy ameliorates stress-impaired dermal wound healing. Brain Behav Immun 2005; 19: 217-222.
25. Leask A, Abraham DJ. The role of connective tissue growth factor, a multifunctional matricellular protein, in fibroblast biology. Biochem Cell Biol 2003; 81: 355-363.
26. Nogales CG, Ferreira MB, Lage-Marques JL. Comparison of the antimicrobial activity of three different concentrations of aqueous ozone on Pseudomonas aeruginosa, Staphylococcus aureus, and Enterococcus faecalis–in vitro study. Revista Española de Ozonoterapia 2014; 4: 9-15.
27. Sadatullah S, Mohamed NH, Razak FA. The antimicrobial effect of 0.1 ppm ozonated water on 24-hour plaque microorganisms in situ. Braz Oral Res 2012; 26: 126-131.
28. Sechi LA, Lezcano I, Nunez N, Espim M, Dupre I, Pinna A, et al. Antibacterial activity of ozonized sunflower oil (Oleozon). J Appl Microbiol 2001; 90: 279-284.
29. Travagli V, Zanardi I, Valacchi G, Bocci V. Ozone and ozonated oils in skin diseases: a review. Mediators Inflamm 2010; 2010: 610418.
30. Travagli V, Zanardi I, Bocci V. Topical applications of ozone and ozonated oils as anti-infective agents: an insight into the patent claims. Recent Pat Antiinfect Drug Discov 2009; 4: 130-142.
31. Kyriazis JD, Aligiannis N, Polychronopoulos P, Skaltsounis A-L, Dotsika E. Leishmanicidal activity assessment of olive tree extracts. Phytomedicine 2013; 20: 275-281.
32. Elamin MH, SS AL-M. Leishmanicidal and apoptotic activities of oleuropein on Leishmania major. Int J Clin Pharmacol Ther 2014; 52: 880-888.
33. Velikaya V, Gribova O, Musabaeva L, Startseva Z, Simonov K, Aleinik AN, et al. Ozone therapy for radiation reactions and skin lesions after neutron therapy in patients with malignant tumors. Vopr Onkol 2014; 61: 571-574.
34. Rosul MV, Patskan BM. Ozone therapy effectiveness in patients with ulcerous lesions due to diabetes mellitus. Wiad Lek 2016; 69: 7-9.
35. Patel PV, Kumar V, Kumar S, Gd V, Patel A. Therapeutic effect of topical ozonated oil on the epithelial healing of palatal wound sites: a planimetrical and cytological study. J Investig Clin Dent 2011; 2: 248-258.
36. Rajabi O, Sazgarnia A, Abbasi F, Layegh P. The activity of ozonated olive oil against Leishmania major promastigotes. Iran J Basic Med Sci 2015; 18: 915-919.
37. Kim HS, Noh SU, Han YW, Kim KM, Kang H, Kim HO, et al. Therapeutic effects of topical application of ozone on acute cutaneous wound healing. J Korean Med Sci 2009; 24: 368-374.
38. Patel PV, Gujjari SK. The morphometrical and histopathological changes which were observed after topical ozone therapy on an exophytic fibrous gingival lesion: A Case Report. J Clin Diagn Res 2013; 7: 1239-1243.
39. Kumar T, Arora N, Puri G, Aravinda K, Dixit A, Jatti D. Efficacy of ozonized olive oil in the management of oral lesions and conditions: A clinical trial. Contemp Clin Dent 2016; 7: 51-54.