Stronger antioxidant enzyme immunoreactivity of Populus tomentiglandulosa extract than ascorbic acid in rat liver and kidney

Document Type: Short Communication

Authors

1 Department of Pharmacy, College of Pharmacy, Dankook University, Cheonan, Chungnam 31116, Republic of Korea

2 Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon, Gangwon 24252, Republic of Korea

3 Division of Food Biotechnology, School of Biotechnology, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea

4 Department of Emergency Medicine, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea

5 Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea

Abstract

Objective(s): Populus species have various pharmacological properties, including antioxidant activity. In this study, the effects of Populus tomentiglandulosa extract (PTE) on histopathology and antioxidant enzymes in the rat liver and kidney were examined.  
Materials and Methods: Sprague-Dawley rats were assigned to three groups; (1) normal diet fed group, (2) ascorbic acid-containing diet-fed group as a positive control, (3) PTE-containing diet-fed group. The histopathology in the rat liver and kidney was examined by hematoxylin and eosin staining. The effect of PTE was examined in the rat liver and kidney by immunohistochemistry for antioxidant enzymes, such as superoxide dismutases (SOD1 and SOD2), catalase (CAT), and glutathione peroxidase (GPx).
Results: No marked histopathological alterations were observed in the liver and kidney of the PTE-containing diet-fed group. In the liver, the mean numbers of SOD1, SOD2, CAT, and GPx immunoreactive cells were significantly increased in the PTE-containing diet-fed rats, compared with those in the normal- and ascorbic acid-containing diet-fed rats. In the kidney, all SOD1, SOD2, CAT, and GPx immunoreactive structures were significantly increased in the PTE-containing diet-fed group, compared with those in the normal- and ascorbic acid-containing diet-fed groups.
Conclusion: Results showed that PTE treatment significantly increased antioxidant enzymes in the rat liver and kidney, and we suggest that PTE might have hepato- and nephro-protective potentials against oxidative stress.

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Main Subjects


1. Alonso-Alconada D, Hilario E, Alvarez FJ, Alvarez A. Apoptotic cell death correlates with ROS overproduction and early cytokine expression after hypoxia-ischemia in fetal lambs. Reprod Sci 2012; 19:754-763.
2. Nita M, Grzybowski A. The role of the reactive oxygen species and oxidative stress in the pathomechanism of the age-related ocular diseases and other pathologies of the anterior and posterior eye segments in adults. Oxid Med Cell Longev 2016; 2016:3164734.
3. Cichoz-Lach H, Michalak A. Oxidative stress as a crucial factor in liver diseases. World J Gastroenterol 2014; 20:8082-8091.
4. Pisani A, Riccio E, Andreucci M, Faga T, Ashour M, Di Nuzzi A, et al. Role of reactive oxygen species in pathogenesis of radiocontrast-induced nephropathy. Biomed Res Int 2013; 2013:868321.
5. Akbulut S, Elbe H, Eris C, Dogan Z, Toprak G, Otan E, et al. Cytoprotective effects of amifostine, ascorbic acid and N-acetylcysteine against methotrexate-induced hepatotoxicity in rats. World J Gastroenterol 2014; 20:10158-10165.
6. Cayir K, Karadeniz A, Simsek N, Yildirim S, Karakus E, Kara A, et al. Pomegranate seed extract attenuates chemotherapy-induced acute nephrotoxicity and hepatotoxicity in rats. J Med Food 2011; 14:1254-1262.
7. Forbes JM, Coughlan MT, Cooper ME. Oxidative stress as a major culprit in kidney disease in diabetes. Diabetes 2008; 57:1446-1454.
8. Yu H, Ge Y, Wang Y, Lin CT, Li J, Liu X, et al. A fused selenium-containing protein with both GPx and SOD activities. Biochem Biophys Res Commun 2007; 358:873-878.
9. Galle J. Oxidative stress in chronic renal failure. Nephrol Dial Transplant 2001; 16:2135-2137.
10. Zhu W, Li YH, Chen ML, Hu FL. Protective effects of Chinese and Brazilian propolis treatment against hepatorenal lesion in diabetic rats. Hum Exp Toxicol 2011; 30:1246-1255.
11. Volpi N, Bergonzini G. Analysis of flavonoids from propolis by on-line HPLC-electrospray mass spectrometry. J Pharm Biomed Anal 2006; 42:354-361.
12. Debbache-Benaida N, Atmani-Kilani D, Schini-Keirth VB, Djebbli N, Atmani D. Pharmacological potential of Populus nigra extract as antioxidant, anti-inflammatory, cardiovascular and hepatoprotective agent. Asian Pac J Trop Biomed 2013; 3:697-704.
13. Dudonne S, Poupard P, Coutiere P, Woillez M, Richard T, Merillon JM, et al. Phenolic composition and antioxidant properties of poplar bud (Populus nigra) extract: individual antioxidant contribution of phenolics and transcriptional effect on skin aging. J Agric Food Chem 2011; 59:4527-4536.
14. Kuchukhidze J, Jokhadze M, Murtazashvili T, Mshvildadze V. Antioxidant polyphenols from Populus alba growing in Georgia. Georgian Med News 2011:94-97.
15. Lee HJ, Kim JS, Kim YK, Ryu JH. Phenolic glycosides as inhibitors of inducible nitric oxide synthase from Populus davidiana in LPS-activated RAW 264.7 murine macrophages. Pharmazie 2012; 67:870-873.
16. Wang K, Ping S, Huang S, Hu L, Xuan H, Zhang C, et al. Molecular mechanisms underlying the in vitro anti-inflammatory effects of a flavonoid-rich ethanol extract from chinese propolis (poplar type). Evid Based Complement Alternat Med 2013; 2013:127672.
17. Zhang X, Thuong PT, Min BS, Ngoc TM, Hung TM, Lee IS, et al. Phenolic glycosides with antioxidant activity from the stem bark of Populus davidiana. J Nat Prod 2006; 69:1370-1373.
18. Park JH, Lee TK, Ahn JH, Shin BN, Cho JH, Kim IH, et al. Pre-treated Populus tomentiglandulosa extract inhibits neuronal loss and alleviates gliosis in the gerbil hippocampal CA1 area induced by transient global cerebral ischemia. Anat Cell Biol 2017; 50:284-292.
19. Lee CH, Park JH, Cho JH, Kim IH, Ahn JH, Lee JC, et al. Effect of Oenanthe Javanica extract on antioxidant enzyme in the rat liver. Chin Med J (Engl) 2015; 128:1649-1654.
20. Tae HJ, Park JH, Cho JH, Kim IH, Ahn JH, Lee JC, et al. Oenanthe javanica extract increases immunoreactivities of antioxidant enzymes in the rat kidney. Chin Med J (Engl) 2014; 127:3758-3763.
21. Si CL, Li SM, Liu Z, Kim JK, Bae YS. Antioxidant phenolic glycosides from the bark of Populus ussuriensis Kom. Nat Prod Res 2011; 25:1396-1401.
22. Karaali HF, Fahmi RR, Borjac JM. Effect of Ocimum basilicum leaves extract on acetaminophen-induced nephrotoxicity in BALB/c mice. J Complement Integr Med 2018; doi: 10.1515/jcim-2018-0111.
23. Joshi D, Srivastav SK, Belemkar S, Dixit VA. Zingiber officinale and 6-gingerol alleviate liver and kidney dysfunctions and oxidative stress induced by mercuric chloride in male rats: A protective approach. Biomed Pharmacother 2017; 91:645-655.