Effect of Scurrula atropurpurea on nitric oxide, endothelial damage, and endothelial progenitor cells of DOCA-salt hypertensive rats

Authors

1 Department of Biology, Faculty of Mathematic and Natural Sciences, Islamic University of Malang, Malang, East Java, Indonesia

2 Department of Pharmacology, Faculty of Medicine University of Brawijaya, Malang, East Java, Indonesia

3 Department of Cardiology and Vascular Medicine, Saiful Anwar General Hospital, Faculty of Medicine, University of Brawijaya, Malang, East Java, Indonesia

Abstract

Objective(s):To know whether Scurrula atropurpurea is able to modulate total plasma nitrate/nitrite levels, decrease endothelial damage, and increase endothelial progenitor cells (EPCs) in hypertensive rats.
Materials and Methods: The rats were divided in 5 groups: control (normotensive) group, Desoxy cortico sterone (DOCA)-salt hypertensive group, and three DOCA-salt hypertensive groups. All 5 groups received methanolic extract of S. atropurpurea (MESA) at a dosage of 50; 100; and 200 mg/KgBW. Serum nitric oxide (NO) was assayed by colorimetric. Circulating endothelial cells (CECs) and EPCs were assayed using flow cytometry.
Results: The administration of MESA100 and MESA200 elevated the total plasma nitrate/nitrite levels but cannot reach the level in control group.  MESA100 and MESA200 also elevated the EPCs number compared with hypertensive group. The administration of MESA significantly (P< 0.05) decreased the CECs number compared to hypertensive groups.
Conclusion: Methanolic extract of S. atropurpurea is able to modulate total plasma nitrate/nitrite levels and diminish endothelial damage via increasing EPCs.

Keywords


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