The Inhibitory Effect of Some Iranian Plants Extracts on the Alpha Glucosidase

Document Type: Original Article


1 Department of Biochemistry, Medical School & Kerman Physiology Research Center, Kerman University of Medical Sciences, Kerman, Iran

2 Department of Pharmacognosy, School of Pharmacy, Kerman University of Medical Science, Kerman, Iran

3 Department of Biology, School of Sciences, Bahonar University, Kerman, Iran


Diabetes mellitus is manifested by hyperglycaemia. Different treatments such as diets and drugs are recommended for diabetes control. For various reasons in recent years traditional plant (herbal) therapies as prescribed by indigenous systems of medicine with different mechanisms have commonly been used. The digestive enzymes such as alpha glucosidase are among these herbal remedies.
Materials and Methods
One hundred species of plants were collected or purchased from the Medicinal Herbal Markets and botanically identified. Methanolic and aqueous extracts were prepared by the maceration method. The enzymatic activities of alpha glucosidase were determined colorimetrically by monitoring the release of       p-nitrophenol from the appropriate p-nitrophenol glycoside substrate, after 30 mins incubation at 37 C in the phosphate buffer (pH= 6.8).
Among 200 prepared extracts, Verbascum kermanensis, Rosa damascene, Rosmarinus officinalis, Levisticum officinale, Zataria multiflora Sanguisorba minor, Alhagi camelorum, Pistacia vera, Vaccinium arcto-staphylus, Zhumeria majdae, Alpinia officinarum, Salvadora persica, and Thymus serpyllum showed more than 50% inhibitory effect on the alpha glucosidase.
These active plants have no records in the literature for their anti diabetic effect and might be the new agents for diabetes control. This needs further in vitro and in vivo studies, some of which are under investigation


1. Melo EBd, Gomesb AdS, Carvalhob I. Alpha- and beta-glucosidase inhibitors: chemical structure and biological activity. Tetrahedron 2006; 62:10277–10302.

2. Zanatta L, de Sousa E, Cazarolli LH, Junior AC, Pizzolatti MG, Szpoganicz B, et al. Effect of crude extract and fractions from Vitex megapotamica leaves on hyperglycemia in alloxan-diabetic rats. J Ethnopharmacol 2007; 109:151-155.

3. Li Y, Wen S, Kota BP, Peng G, Li GQ, Yamahara J, et al. Punica granatum flower extract, a potent alpha-glucosidase inhibitor, improves postprandial hyperglycemia in Zucker diabetic fatty rats. J Ethnopharmacol 2005; 99:239-244.

4. Ortiz-Andrade RR, Garcia-Jimenez S, Castillo-Espana P, vila GR-A, Villalobos-Molina R, Estrada-Soto S. Alpha-Glucosidase inhibitory activity of the methanolic extract from Tournefortia hartwegiana: An anti-hyperglycemic agent. J Ethnopharmacol 2007; 109: 48–53.

5. Shim YJ, Doo HK, Ahn SY, Kim YS, Seong JK, Park IS, et al. Inhibitory effect of aqueous extract from the gall of Rhus chinensis on alpha-glucosidase activity and postprandial blood glucose. J Ethnopharmacol 2003; 85:283-287.

6. Pluempanupat W, Adisakwattana S, Yibchok-Anun S, Chavasiri W. Synthesis of N-phenylphthalimide derivatives as alpha-glucosidase inhibitors. Arch Pharm Res 2007; 30:1501-1506.

7. Bhandari MR, Jong-Anurakkun N, Hong G, Kawabata J. A-glucosidase and a-amylase inhibitory activities of Nepalese medicinal herb Pakhanbhed (Bergenia ciliata, Haw.). Food Chem 2008; 106:247–252

8. Bhandari MR, Jong-Anurakkun N, Hong G, Kawabata J. A-Glucosidase and a-amylase inhibitory activities of Nepalese medicinal herb Pakhanbhed (Bergenia ciliata, Haw.). Food Chem 2008; 106:247–252.

9. Duncan AC, Jager AK, Vanstaden J. Screening of Zulu medicinal plants for angiotensin converting enzyme (ACE) inhibitors. J Ethnopharmacol 1999; 68:63-70.

10.  Cope WA, Bell TA, Smart WWG. Changes in an enzyme inhibitor and tannin content in Serica lespezda. Crop Sci Society Am 1971; 11:893-895.

11.  Sepici A, Gurbuz I, Cevik C, Yesilada E. Hypoglycaemic effects of myrtle oil in normal and alloxan-diabetic rabbits. J Ethnopharmacol 2004; 93:311-318.

12.  Sabu MC, Kuttan R. Anti-diabetic activity of medicinal plants and its relationship with their antioxidant property. J Ethnopharmacol 2002; 81:155-160.

13.  Mackenzie T, Leary L, Brooks WB. The effect of an extract of green and black tea on glucose control in adults with type 2 diabetes mellitus: double-blind randomized study. Metabolism 2007; 56:1340-1344.

14.  Sabu MC, Smitha K, Kuttan R. Anti-diabetic activity of green tea polyphenols and their role in reducing oxidative stress in experimental diabetes. J Ethnopharmacol 2002; 83:109-116.

15.  Afifi FU, Al-Khalidi B, Khalil E. Studies on the in vivo hypoglycemic activities of two medicinal plants used in the treatment of diabetes in Jordanian traditional medicine following intranasal administration. J Ethnopharmacol 2005; 100:314 -318.

16. Romani A, Coinu R, Carta S, Galardi C, Vincieri FF, Franconi F. Evaluation of antioxidant effect of different extract of Myrtus communis L. Free Radic Res 2004; 38:97-103

17.  Swanston-Flatt SK, Day C, Bailey CJ, Flatt PR. Traditional plant treatments for diabetes. Studies in normal and streptozotocin diabetic mice. Diabetologia 1990; 33:462-464.

18.  Kimura I, Nakashima N, Sugihara Y, Fu-jun C, Kimura M. The antihyperglycaemic blend effect of traditional chinese medicine byakko-ka-ninjin-to on alloxan and diabetic KK-CA(y) mice. Phytother Res 1999; 13:484-488.

19.  Glombitza KW, Mahran GH, Mirhom YW, Michel KG, Motawi TK. Hypoglycemic and antihyperglycemic effects of Zizyphus spina-christi in rats. Planta Med 1994; 60:244 -247.

20.  Gheibi N, Hashemi HJ, Parvizi M. the effect of cinnamon on glucose concentration of diabetic rats in presence or absence of insulin. Qazvin Univ. Med. Sci 2005; 9:2-7.

21.  Swanston-Flatt SK, Day C, Flatt PR, Gould BJ, Bailey CJ. Glycaemic effects of traditional European plant treatments for diabetes. Studies in normal and streptozotocin diabetic mice. Diabetes Res 1989; 10:69-73.

22.  Narendhirakannan RT, Subramanian S, Kandaswamy M. Mineral content of some medicinal plants used in the treatment of diabetes mellitus. Biol Trace Elem Res 2005; 103:109-115.

23.  Saxena A, Vikram NK. Role of selected Indian plants in management of type 2 diabetes: a review. J Altern Complement Med 2004; 10:369-378.

24.  Farzami B, Ahmadvand D, Vardasbi S, Majin FJ, Khaghani S. Induction of insulin secretion by a component of Urtica dioica leave extract in perifused islets of Langerhans and its in vivo effects in normal and streptozotocin diabetic rats. J Ethnopharmacol 2003; 89:47-53.

25.  Pérez C, Dom?nguez E, Ramiro JM, Romero A, Campillo JE, Torres MD. A study on the glycaemic balance in streptozotocin-diabetic rats treated with an aqueous extract of Ficus carica (fig tree) leaves. Phytother Res 1998; 10:82-83.

26.  Hussain Z, Waheed A, Qureshi RA, Burdi DK, Verspohl EJ, Khan N, et al. The effect of medicinal plants of Islamabad and Murree region of Pakistan on insulin secretion from INS-1 cells. Phytother Res 2004; 18:73-77.

27.  Broadhurst CL, Polansky MM, Anderson RA. Insulin-like biological activity of culinary and medicinal plant aqueous extracts in vitro. J Agric Food Chem 2000; 48:849-852.

28.  Esmaeili MA, Yazdanparast R. Hypoglycaemic effect of Teucrium polium: studies with rat pancreatic islets. J Ethnopharmacol 2004; 95:27-30.

29.  Kar A, Choudhary BK, Bandyopadhyay NG. Comparative evaluation of hypoglycaemic activity of some Indian medicinal plants in alloxan diabetic rats. J Ethnopharmacol 2003; 84:105-108.

30.  Talpur N, Echard B, Ingram C, Bagchi D, Preuss H. Effects of a novel formulation of essential oils on glucose-insulin metabolism in diabetic and hypertensive rats: a pilot study. Diabetes Obes Metab 2005; 7:193-199.

31.  Hwang JK, Kong TW, Baek NI, Pyun YR. Alpha-glycosidase inhibitory activity of hexagalloylglucose from the galls of Quercus infectoria. Planta Med 2000; 66:273-274.