Breviscapine prevents downregulation of renal water and sodium transport proteins in response to unilateral ureteral obstruction

Document Type : Short Communication

Authors

1 Department of Intensive Care Unit, The First Affiliate Hospital of Wenzhou Medicine University, China

2 Department of Transplantation Center, the First Affiliate Hospital of Wenzhou Medicine University, China

3 Key Laboratory of Surgery, Department of Surgery, The First Affiliated Hospital of Wenzhou Medicine University, China

Abstract

Objective(s):Our recent report indicates that breviscapine play a protective role of the kidney by down-regulating transforming growth factor-β1(TGF-β1), α-smooth muscle actin (α-SMA) and alleviating interstitial fibrosis following unilateral ureteral obstruction (UUO). In this study, we investigate the effect of breviscapine on changes of renal water and sodium transport proteins in response to UUO.
Materials and Methods: Male Sprague-Dawley rats were divided into 3 groups, sham group, UUO group and UUO treat with breviscapine. After 4, 7 and 14 days, histologic changes and interstitial collagen were determined microscopically following hematoxylin and eosin (H&E) and Masson's trichrome staining. The expression of Aquaporins (AQP-2) and γ-epithelial sodium channel (γ-ENaC) were investigated using immunohistochemistry and Western blot in each group.
Results:Breviscapine treatment decrease the tubular injury index and the degree of interstitial collagen deposition significantly compared with the UUO group (P<0.05). Breviscapine treatment also significantly reduced downregulation of AQP2 and γ-ENaC compared to those subjected to the same time course of obstruction in UUO group (P<0.05). 
Conclusion:These results demonstrate that breviscapine could prevent downregulation of renal water and sodium transport proteins in response to UUO so as to protect obstructed kidney.

Keywords

References

1. Nath KA. Heme oxygenase-1 and acute kidney injury. Curr Opin Nephrol Hypertens 2014; 23:17-24.
2. Muto S, Asano Y. Electrical properties of the rabbit cortical collecting duct from obstructed kidneys after unilateral ureteral obstruction. Effects of renal decapsulation. J Clin Invest 1994; 94:1846-1854.
3. Wang G, Yuan W, Kwon TH, Li Z, Wen J, Topcu SO, et al. Age-related changes in expression in renal AQPs in response to congenital, partial, unilateral ureteral obstruction in rats. Pediatr Nephrol 2012; 27:83-94.
4. Li C, Wang W, Norregaard R, Knepper MA, Nielsen S, Frokiaer J. Altered expression of epithelial sodium channel in rats with bilateral or unilateral ureteral obstruction. Am J Physiol Renal Physiol 2007; 293:F333-341.
5. Xiong F, Wang H, Geng KK, Gu N, Zhu JB. Optimized preparation, characterization and biodistribution in heart of breviscapine lipid emulsion. Chem Pharm Bull 2010; 581:1455-1460.
6. Wei L, Tan J. Clinical observation on Breviscapine in treating hypertension patients complicated with micro-albuminuria of renal impairment. Chin J Integr Med 2005 ; 11:31-33.
7. Qi XM, Wu GZ, Wu YG, Lin H, Shen JJ, Lin SY. Renoprotective effect of breviscapine through suppression of renal macrophage recruitment in streptozotocin-induced diabetic rats. Nephron Exp Nephrol 2006; 104:e147-157.
8. Pelaez LI, Juncos LA, Stulak JM, Lerman LO, Romero JC. Non-invasive evaluation of bilateral renal regional blood flow and tubular dynamics during acute unilateral ureteral obstruction. Nephrol Dial Transplant 2005; 20:83-88.
9. Klahr S. New insights into the consequences and mechanisms of renal impairment in obstructive nephropathy. Am J kidney Dis 1991; 18:689-699.
10. Lee MD, King LS, Agre P. The aquaporin family of water channel proteins in clinical medicine. Medicine 1997; 76:141-156.
11. Nielsen S. Renal aquaporins: an overview. BJU Int 2002; 90:1-6.
12. Wood-Bradley RJ, Barrand S, Giot A, Armitage JA. Understanding the role of maternal diet on kidney development; an opportunity to improve cardiovascular and renal health for future generations. Nutrients 2015; 7:1881-1905.
13. DiGiovanni SR, Nielsen S, Christensen EI, Knepper MA. Regulation of collecting duct water channel expression by vasopressin in Brattleboro rat. Proc Natl Acad Sci U S A 1994; 91:8984-8988.
14. Nielsen S, Kwon TH, Frokiaer J, Knepper MA. Key roles of renal aquaporins in water balance and water-balance disorders. News Physiol Sci 2000; 15:136-143.
15. Ishikawa S. Urinary excretion of aquaporin-2 in pathological states of water metabolism. Ann Med 2000; 32:90-93.
16. Jensen JM, Mose FH, Kulik AE, Bech JN, Fenton RA, Pedersen EB. Changes in urinary excretion of water and sodium transporters during amiloride and bendroflumethiazide treatment. World J Nephrol 2015; 4:423-437.
17. Canessa CM, Schild L, Buell G, Thorens B, Gautschi I, Horisberger JD, et al. Amiloride-sensitive epithelial Na+ channel is made of three homologous subunits. Nature 1994; 367:463-467.
18. Inagaki A, Yamaguchi S, Ishikawa T. Amiloride-sensitive epithelial Na+ channel currents in surface cells of rat rectal colon. Am J Physiol Cell Physiol 2004; 286:C380-390.
19. Pochynyuk O, Bugaj V, Rieg T, Insel PA, Mironova E, Vallon V, et al. Paracrine regulation of the epithelial Na+ channel in the mammalian collecting duct by purinergic P2Y2 receptor tone.  J Biol Chem 2008; 283:36599-36607.
20. Li C, Wang W, Kwon TH, Knepper MA, Nielsen S, Frokiaer J. Altered expression of major renal Na transporters in rats with unilateral ureteral obstruction. Am J Physiol Renal Physiol 2003; 284:F155-166.
21. Konstas AA, Korbmacher C. The gamma-subunit of ENaC is more important for channel surface expression than the beta-subunit. Am J Physiol Cell Physiol 2003; 284:C447-456.
22. Li C, Wang W, Kwon TH, Isikay L, Wen JG, Marples D, et al. Downregulation of AQP1, -2, and -3 after ureteral obstruction is associated with a long-term urine-concentrating defect. Am J Physiol Renal Physiol 2001; 281:F163-171.
23. Lin LL, Liu AJ, Liu JG, Yu XH, Qin LP, Su DF. Protective effects of scutellarin and breviscapine on brain and heart ischemia in rats. J Cardiovasc Pharm 2007; 50:327-332.
24. Yan L, Huang H, Tang QZ, Zhu LH, Wang L, Liu C, et al. Breviscapine protects against cardiac hypertrophy through blocking PKC-alpha-dependent signaling. J Cell Biochem 2010; 109:1158-1171.
25. Wang M, Zhang WB, Zhu JH, Fu GS, Zhou BQ. Breviscapine ameliorates cardiac dysfunction and regulates the myocardial Ca(2+)-cycling proteins in streptozotocin-induced diabetic rats. Acta Diabetol 2010; 47:209-218.
26. Wang C, Li Y, Gao S, Cheng D, Zhao S, Liu E. Breviscapine injection improves the therapeutic effect of western medicine on angina pectoris patients. PloS One 2015; 10:e0129969.
27. Wu YG, Xia LL, Lin H, Zhou D, Qian H, Lin ST. Prevention of early liver injury by breviscapine in streptozotocin-induced diabetic rats. Planta Med 2007; 73:433-438.
28. Zhang SJ, Song Y, Zhai WL, Shi JH, Feng LS, Zhao YF, et al. Breviscapine alleviates hepatic injury and inhibits PKC-mRNA and its protein expression in brain-dead BA-Ma mini pigs. Hepatobiliary Pancreat Dis Int 2007; 6:604-609.
 
Iranian Journal of Basic Medical Sciences
Volume 19, Issue 5
May 2016
Pages 573-578

Files

Share

How to cite

Statistics