Volatile compounds analysis and antioxidant, antimicrobial and cytotoxic activities of Mindium laevigatum

Document Type: Original Article

Authors

1 Essential Oils Research Institute, University of Kashan, Kashan (Qamsar), Iran

2 Kashan Botanical Garden, Isfahan Center for Research and Education of Agricultural Science and Natural Resources, Isfahan, Iran

3 Department of Analytical Chemistry, Faculty of Chemistry, University of Kashan, Kashan, Iran

Abstract

Objective(s): Mindium laevigatum is an endemic plant of Iran and Turkey and is widely used as blood purifier, antiasthma and antidyspnea in traditional medicine. Chemical composition of volatile materials of the plant and its antioxidant, antimicrobial and cytotoxic activities were reported in this study.
Materials and Methods: Simultaneous distillation-extraction (SDE) and GC-Mass-FID analysis were used for the plant volatile materials chemical composition identification and quantification. Several antioxidant tests including DPPH radical scavenging, hydrogen peroxide scavenging, reducing power determination, β-carotene-linoleic acid and total phenolic content tests were used for antioxidant activity evaluation. Antimicrobial and anticancer activities were also estimated using microbial strains, cancer cell lines and brine shrimp larva.
Results: GC-Mass-FID analysis of volatile samples showed a total of 74 compounds of which palmitic acid (7.4-33.7%), linoleic acid (6.6-18.6%), heneicosane (1.3-9.6%) and myristic acid (1.4-6.0%) were detected as main volatile components. Moderate to good results were recorded for the plant in              β-carotene-linoleic acid test. Total phenolic content of the extracts as gallic acid equivalents were estimated in the range of 15.7 to 79.6 μg/mg. Some microbial strains showed moderate sensitivities to plant extracts. Brine shrimp lethality test and cytotoxic cancer cell line assays showed mild cytotoxic activities for the plant.
Conclusion: Moderate to good antioxidant activities in β-carotene-linoleic acid test and presence of considerable amounts of unsaturated hydrocarbons may explain the plant traditional use in asthma and dyspnea. These findings also candidate it as a good choice for investigating its possible modern medical applications.

Keywords


1. Colegate SM, Molyneux RJ. Bioactive natural products: detection, isolation, and structural determination: CRC press; 2008.

2. Aghabeigi F, Jalilian N. Flora of Iran. Campanulaceae, .Research Institute of Forests and Rangelands; 2010; 66.

3. Aslan S, Vural M, Şahin B, Ergin E, Kaya ÖF. A new record for Turkey: Michauxia nuda A. DC. (Campanulaceae).Turk J Bot 2010; 34:51-56.

4. Ghahraman A. Plant systematic: coromophytes of Iran. Tehran, Iran University press; 1990. p. 407-417.

5. Güvenç A, Akkol EK, Hürkul MM, Süntar İ, Keleş H. Wound healing and anti-inflammatory activities of the Michauxia L’Hérit (Campanulaceae) species native to Turkey.J Ethnopharmacol 2012; 139:401-408.

6. Touafek O, Kabouche Z, Brouard I, Barrera Bermejo J. Flavonoids of Campanula alata and their antioxidant activity.Chem Nat Comp 2011; 46:968-9670.

7. Mozaffarian V. A Dictionary of Iranian Plant Names.Tehran: Farhang Moaser;1996.p.349.

8. Mosaddegh M, Naghibi F, Moazzeni H, Pirani A, Esmaeili S. Ethnobotanical survey of herbal remedies traditionally used in Kohghiluyeh va Boyer Ahmad province of Iran.J Ethnopharmacol 2012; 141:80-95.

9. Brunton LL, Lazo JS, Parker KL. Goodman & Gilman's the pharmacological basis of therapeutics. McGraw-Hill Publishing; 2006.

10. Seidel V, Lindner W. Universal sample enrichment technique for organochlorine pesticides in environmental and biological samples using a redesigned simultaneous steam distillation-solvent extraction apparatus.Anal Chem 1993; 65:3677-3683.

11. Adams RP. Identification of essential oil components by gas chromatography/mass spectrometry: Carol Stream, Illinois: Allured publishing corporation; 2007.

12. Sarker SD, Latif Z, Gray AI. Natural products isolation: Springer Science & Business Media; 2006.

13. Singh HP, Mittal S, Kaur S, Batish DR, Kohli RK. Chemical composition and antioxidant activity of essential oil from residues of Artemisia scoparia.Food Chem 2009; 114:642-645.

14. Tounsi MS, Ouerghemmi I, Wannes WA, Ksouri R, Zemni H, Marzouk B, et al. Valorization of three varieties of grape.Ind Crops Prod 2009; 30:292-296.

15. Miraliakbari H, Shahidi F. Antioxidant activity of minor components of tree nut oils.Food Chem 2008; 111:421-427.

16. Slinkard K, Singleton VL. Total phenol analysis: automation and comparison with manual methods.Am J Enol Vitic 1977; 28:49-55.

17. Meyer B, Ferrigni N, Putnam J, Jacobsen L, Nichols Dj, McLaughlin J. Brine shrimp: a convenient general bioassay for active plant constituents.Planta Med 1982:31-34.

18. Scudiero DA, Shoemaker RH, Paull KD, Monks A, Tierney S, Nofziger TH, et al. Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines.Cancer Res 1988; 48:4827-4833.

19. Murray PR, Baron EJ, Pfaller M, Tenover F, Yolken RH, Morgan DR. Manual of clinical microbiology (6th edn).Trends Microbiol 1995; 3:449.

20. Güllüce M, Sökmen M, Şahin F, Sökmen A, Adigüzel A, Özer H. Biological activities of the essential oil and methanolic extract of Micromeria fruticosa (L) Druce ssp serpyllifolia (Bieb) PH Davis plants from the eastern Anatolia region of Turkey.J Sci Food Agric 2004; 84:735-741.

21. Baser HCK , Buchbauer G. Handbook of Essential Oils, Science, Technology and Applications. CRC Press, Taylor & Francis Group, Boca Raton, FL; 2010.

22. Masoum S, Ghasemi-Estarki H, Seifi H, Ebrahimabadi EH, Parastar H. Analysis of the volatile chemical constituents in Mindium laevigatum by gas chromatography—Mass spectrometry and correlative chemometric resolution methods.Microchem J 2013; 106:276-281.

23. Modaressi M, Shahsavari R, Ahmadi F, Rahimi-Nasrabadi M, Abiri R, Mikaeli A, et al. The evaluation of antibacterial, antifungal and antioxidant activity of methanolic extract of Mindium Laevigatum (Vent.) Rech. F, From Central Part of Iran.Jundishapur J Nat Pharm Prod 2013; 8:34.