Berberis vulgaris: specifications and traditional uses

Authors

1 Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran

2 Department of Chemistry, Faculty of Sciences, Shahrekord University, Shahrekord, Iran

Abstract

The medicinal plants from genus Berberis are particularly important in traditional medicine and the food basket of Iranians. Given various plants from genus Berberis and their economic, nutritional, and medicinal status in Iran, this study seeks to investigate the findings of recent studies on the phytochemical characteristics, specifications, and uses of Berberis vulgaris. In this review article, 350 articles were initially retrieved from reliable scientific databases using relevant search terms. Then, 230 articles were selected and 120 were excluded after a primary analysis. Finally, 98 articles related to the subject under study were meticulously examined and the required data were extracted and classified according to the research purposes. The findings were divided into eight separate sections: Introducing Berberidaceae family, different species of Berberis, pharmaceutical organs, B. vulgaris nutrition facts and minerals, the antioxidants and alkaloids compounds in fruit and other organs, action mechanisms of preventing and treating diseases, traditional uses of B. vulgaris, and its properties reported by recent studies. The results briefly indicate that B. vulgaris contains a large number of phytochemical materials including ascorbic acid, vitamin K, several triterpenoids, more than 10 phenolic compounds and more than 30 alkaloids. Therefore B. vulgaris may have anti-cancer, anti-inflammatory, antioxidant, antidiabetic, antibacterial, analgesic and anti-nociceptive and hepato-protective effects. Regarding the use of different organs of B. vulgaris in traditional medicine and their confirmed effects in the recent studies, it is possible to use different organs of B. vulgaris, especially fruit, to develop new drugs.

Keywords


1. Bahmani M, Zargaran A, Rafieian-Kopaei M, Saki K. Ethnobotanical study of medicinal plants used in the management of diabetes mellitus in the Urmia, Northwest Iran. Asian Pac J Trop Med 2014;7:S348-S354.

2. Calixto J. Efficacy, safety, quality control, marketing and regulatory guidelines for herbal medicines (phytotherapeutic agents). Braz J Med Biol Res 2000;33:179-189.

3. Fallah Huseini H, Zareei Mahmoudabady A, Mehrazma M, Alavian SM, Kianbakht S, Mehdizadeh M. The effects of taraxacum officinale L. and Berberis vulgaris L. root extracts on carbon tetrachloride induced liver toxicity in rats. J Med Plan 2010; 9:45-52.

4. Hasani-Ranjbar S, Larijani B, Abdollahi M. A systematic review of Iranian medicinal plants useful in diabetes mellitus.Arch Med Sci 2008; 4:285-292.

5. Shamsa F, Ahmadiani A, Khosrokhavar R. Antihistaminic and anticholinergic activity of barberry fruit (Berberis vulgaris) in the guinea-pig ileum. J Ethnopharmacol 1999; 64:161-166.

6. Alemardan A, Asadi W, Rezaei M, Tabrizi L, Mohammadi S. Cultivation of Iranian seedless barberry (Berberis integerrima ‘Bidaneh’): A medicinal shrub. Ind Crops Prod 2013; 50:276-287.

7. Hesami M, Aliabaday S. Berberis use traditional Drtb. National Seminar on Natural Products and Medicinal Plants, J North Khorasan Univ Med Sci 2012; Exclusive Magazine Conference: p. 31.

8. Mozaffarian V. A Dictionary of Iranian Plant Names. Tehran-Iran: Farhang Moaser; 2007.

9. Ardestani SB, Sahari MA, Barzegar M, Abbasi S.Some physicochemical properties of Iranian native barberry fruits (abi and poloei): Berberis integerrima and Berberis vulgaris. J Food Pharm Sci 2013; 1:3.

10. Rahimi-Madiseh M, Gholami-Arjenaki M, Bahmani M, Mardani G, Farzan M, Rafieian-Kopaei M.  Evaluation of minerals, phenolics and anti-radical activity of three species of Iranian berberis fruit. Derpharma Chemica.2016; 8:191-197.

11. Zarghami Moghadam M. Barberry ecophysiology. Environ Iran 2011;  24:50-51.

12. Mokhber-Dezfuli N, Saeidnia S, Gohari AR, Kurepaz-Mahmoodabadi M.  Phytochemistry and pharmacology of berberis species. Pharmacogn Rev 2014; 8:8.

13. Bhardwaj D, Kaushik N. Phytochemical and pharmacological studies in genus Berberis. Phytochem Rev 2012; 11:523-542.

14. Abd El-Wahab AE, Ghareeb DA, Sarhan EE, Abu-Serie MM, El Demellawy MA. In vitro biologicalassessment of Berberis vulgaris and its active constituent, berberine: antioxidants, anti-acetylcholinesterase, anti-diabetic and anticancer effects. BMC Complement Altern Med 2013; 13:1.

15. Farhadi AK, Gavadifar K, Farha A.Effects of Berberise Vulgaris fruit extract on blood cholesterol and triglyceride in hyperlipidemic patients. Koomesh, J Semnan Univ Med Sci 2008; 9:211-216.

16. Rezaei M, Ebadi A, Reim S, Fatahi R, Balandary A, Farrokhi N, et al. Molecular analysis of Iranian seedless barberries via SSR. Sci Hortic 2011; 129: 702-709.

17. Roy S, Tyagi A, Shukla V, Kumar A, Singh UM, Chaudhary LB, et al. Universal plant DNA barcode loci may not work in complex groups: a case study with Indian Berberis species. PloS One 2010; 5:e13674..

18. Singh M, Srivastava S, Rawat A. Antimicrobial activities of Indian Berberis species. Fitoterapia 2007; 78:574-576.

19. Ghahreman A. Flora of Iran. Tehran-Iran: Research institute of forests and rangelands; Vol. 7. 1987.

20. Mozaffarian V. Trees and Shrubs of Iran. Tehran-Iran: Farhang Moaser; 2005.

21. Mazandarani M, Ghasemi N,  Bayat H. The second review and comparison of active ingredients in plant organs of Berberis vulgaris L. J Plant Sci Res2013 ; 8:59-17.

22. Khan T, Khan IA, Rehman A. Evaluation and detailing of taxonomic and historical perspectives on genus Berberis from Pakistan. J Bio & Env Sci 6: 361-367.

23. Christenhusz JM, Byng JW. The number of known plants species in the world and itsannual increase. Phytotaxa 2016; 261:201-217.

24. Landrum LR. Revision of Berberis (Berberidaceae) in Chile and adjacent southern Argentina. Ann Mo Bot Gard 1999: 793-834.

25. Laamech, J. Laamech J, El-Hilaly J, Fetoui H, Chtourou Y, Gouitaa H, Tahraoui A, Lyoussi B. "Berberis vulgaris L. effects on oxidative stress and liver injury in lead-intoxicated mice." J Complement Integr Med 2017; 14. DOI: 10.1515/jcim-2015-0079

26. Nickol MG. Phylogeny and inflorescences of Berberidaceae—a morphological survey, in Systematics and Evolution of the Ranunculiflorae. Springer; 1995.p. 327-340.

27. Ahrendt LWA. Berberis and Mahonia. Bot J Linn Soc 1961; 57:1-410. DOI: 10.1111/j.1095-8339.1961. tb00889.x

28. Sodagar N, Bahrami AR, Memariani F, Ejtehadi H, Vaezi J, Khosravi AR. Biosystematic study of the genus Berberis L.(Berberidaceae) in Khorassan, NE Iran. Plant Syst Evol 2012; 298:193-203.

29. Madiseh MR, Heidarian E, Rafieian-kopaei M. Biochemical components of Berberis lycium fruit and its effects on lipid profile in diabetic rats. J HerbMed Pharmacol 2014; 3:1.

30. Yin J, Hu R, Chen M, Tang J, Li F, Yang Y, et al. Effects of berberine on glucose metabolism in vitro. Metabolism 2002; 51:1439-1443.

31. Farhady Chitgar M, Varidy MJ, Varidy M. Evaluation of some physicochemical properties of Berberis crtagina. 2012, Bojnord-Iran: Book of the National Conference of natural products and herbs.

32. Rajurkar NS, Pardeshi BM. Analysis of some herbal plants from India used in the control of diabetes mellitus by NAA and AAS techniques. Appl Rad Isot 1997; 48:1059-1062.

33. Arayne MS, Sultana N, Bahadur SS. The berberis story: Berberis vulgaris in therapeutics. Pak J Pharm Sci 2007; 20:83-92.

34. Zarei A, Changizi-Ashtiyani S, Taheri S, Ramezani M. A quick overview on some aspects of endocrino-logical and therapeutic effects of Berberis vulgaris L. Avicenna J Phytomed 2015; 5: 485.

35. Hadaruga DI, Hadaruga NG, Bandur GN, Rivis A, Costescu C, Ordodi VL, et al. Berberis vulgaris extract/β cyclodextrin nanoparticles synthesis and characterization. Rev Chim(Bucharest) 2010; 61:669-675.

36. Kong W, et al. Berberine is a novel cholesterol-lowering drug working through a unique mechanism distinct from statins. Nat Med 2004; 10:1344-1351.

37. Fatehi-Hassanabad Z, Jafarzadeh M, Tarhini A, Fatehi M. The antihypertensive and vasodilator effects of aqueous extract from Berberis vulgaris fruit on hypertensive rats. Phytother Res 2005; 19: 222-225.

38. Arayne MS, Sultana N, Bahadur SS. The berberis story: Berberis vulgaris in therapeutics. Pak J Pharm Sci 2007; 20:83-92.

39. Küpeli E, Koşar M, Yeşilada E, Hüsnü K, Başer C. A comparative study on the anti-inflammatory, antinociceptive and antipyretic effects of isoquinoline alkaloids from the roots of Turkish Berberis species. Life Sci 2002; 72:645-657.

40. Laferriere JE, Weber CE, Kohlhepp EA. Useand nutritional composition of some traditional Mountain Pima plant foods. J Ethnobiol 1991; 11:93-114.

41. Hunt AH. The role of vitamin C in wound healing. Br J Surg 1941; 28:436-461.

42. Pai K, Srilatha P, Suryakant K, Setty MM, Nayak PG, Rao CM, et al.Anticancer activity of Berberis aristata in Ehrlich ascites carcinoma-bearing mice: A preliminary study. Pharm Biol 2012; 50:270-277.

43. Carr AC, Frei B. Toward a new recommended dietary allowance for vitamin C based on antioxidant and health effects in humans.  Am J Clin Nutr 1999; 69:1086-1107.

44. Bsoul S,  Terezhalmy G. Vitamin C in health and disease. J Contemp Dent Pract 2004; 5:1-13.

45. Aghili M. Makhzan-al-Advia. Tehran: Tehran University of Medical Sciences; 2009.p.328.

46. Razi M. Al-havi. Tehran, Iran: Academy of Medical Sciences Islamic Republic of Iran; 2005.p.227.

47. Villinski J, et al. Antibacterial activity and alkaloid content of berberis thunbergii, berberis vulgaris and hydrastis canadensis. Pharm Biol 2003; 41:551-557.

48. Javadzadeh SM, Fallah SR. Therapeutic application of different parts berberis vulgaris. Int J Agri Crop Sci 2012; 4:404-408.

49. Shirzad H, Taji F. Rafieian-Kopaei F. Correlation between antioxidant activity of garlic extracts and WEHI-164 fibrosarcoma tumor growth in BALB/c mice. J Med Food 2011; 14:969-974.

50. Ivanovska N, Philipov S. Study on the anti-inflammatory action of Berberis vulgaris root extract, alkaloid fractions and pure alkaloids. Int J Immunopharmacol 1996; 18:553-561.

51. Singh, Kakkar P. Antihyperglycemic and antioxidant effect of Berberis aristata rootextract and its role in regulating carbohydrate metabolism in diabetic rats. J Ethnopharmacol 2009; 123:22-26.

52. Bashir S, Gilani AH, Siddiqui AA, Pervez S, Khan SR, Sarfaraz NJ, et al. Berberis vulgaris root bark extract prevents hyperoxaluria induced urolithiasis in rats. Phytother Res 2010; 24:1250-1255.

53. Kosalec I, Gregurek B, Kremer D, Zovko M, Sanković K, Karlović K. Croatian barberry (Berberis croatica Horvat): a new source of berberine—analysis and antimicrobial activity. World J Microbiol Biotechnol 2009; 25:145-150.

54. Hosseinzadeh H. Anticonvulsant effect of berberis integerrima L. root extracts in mice. J Acupunct Meridian Stud 2013; 6:12-17.

55. Chueh WH, Lin JY. Berberine, an Isoquinoline Alkaloid in Herbal Plants, Protects Pancreatic Islets and Serum Lipids in Nonobese Diabetic Mice. J Agric Food Chem 2014; 59:8021-8027.

56. Landmark K. [Could intake of vitamins C and E inhibit development of Alzheimer dementia?]. Tidsskr Nor Laegeforen 2006; 126:159-161.

57. Harrison FE, Hosseini AH, McDonald MP, May JM. Vitamin C reduces spatial learning deficits in middle-aged and very old APP/PSEN1 transgenic and wild-type mice. Pharmacol Biochem Behav 2009; 93:443-450.

58. Levine M, Rumsey SC, Daruwala R, Park JB, Wang Y. CRiteria and recommendations for vitamin c intake. JAMA 1999; 281:1415-1423.

59. Cho J, Kang J, Long P, Jing J, Back Y, Chung K-S. Antioxidant and memory enhancing effects of purple sweet potato anthocyanin and cordyceps mushroom extract. Arch Pharm Res 2003; 26:821-825.

60. Ahangarpour A, Oroojan AA, Radan M. Effect of aqueous and hydroalcoholic extract of Beberis vulgaris on insulin secretion from islets of langerhans isolated from male mice. Armaghane-danesh, J Yasuj Univ Med Sci 2012; 17:289-298.

61. Ebrahimi M, Arefhosseini, Golzarand, Aliasgarzadeh, Vahed J. Long-term effects of processed berberis vulgaris on some metabolic syndrome components. Iran J Endocrinol Metab 2009; 11:41-47.

62. Golzarand M, Ebrahimi-Mamaghani M, Arefhosseini R, Aliasgarzadeh A.  Short term-effect of processed berberis consumption vulgaris on cardiovascular risk factors in type II diabetes patients with metabolic syndrome. Med J Tabriz Univ Med Sci Health Services 2009; 31:89-94.

63. Farhadi A, Gavadifar K, Farhadi A. Effects of Berberise vulgaris fruit extract on blood cholesterol and triglyceride in hyperlipidemic patients. koomesh 2008; 9:211-216.

64. Hermenean A, Popescu C, Ardelean A, Stan M, Hadaruga N, Mihali CV, et al. Hepatoprotective effects of Berberis vulgaris L.extract/β cyclodextrin on carbon tetrachloride–induced acute toxicity in mice. Int J Mol Sci 2012; 13:9014-9034.

65. Eshraghi A, Movahedian AA, Asgari S, Naderi GA, Badiee A.  Antioxidant Effect of Ziziphus vulgaris, Portulaca oleracea, Berberis integerima and gundelia tournefortti on lipid peroxidation, Hb glycosylation and Red Blood Cell Hemolysis. J Med Plants 2011; 10:80-88.

66. Fouladi RF. Aqueous Extract of dried fruit of Berberis vulgaris L. in Acne vulgaris, a clinical trial. J Diet Suppl 2012; 9:253-61.

67. Meshkibaf MH, Abdollahi A, Fasihi RM, Adnani SJ, Moravvej A, Hatami S. Antibacterial effects of hydro-alcoholic extracts of Ziziphora tenuior, Teucrium polium, Barberis corcorde and Stachys inflate. koomesh J Semnan Univ Med Sci 2010; 11:240-244.

68. Salar F, Ziai SA, Nasri S, Roghani M, Kamalinejad M. Neuroprotective Effect of aqueous extract of Berberis vulgaris L. in a model of parkinson’s disease in rat. J Med Plants 2010; 4: 24-33.

69. Cui G, Qin X, Zhang Y, Gong Z, Ge B, Zang YQ. Berberiane differentially modulates the activities of ERK,p38 MPK,and JNK to suppress Th17 and Th1 cell diffrentiation in type 1 diabetic mice. J Biol chem 2009; 284:28420-28429.

70. Bashir S, Gilani AH, Siddiqui AA, Pervez S, Khan SR, Sarfaraz NJ, et al. Berberis vulgaris root bark extract prevents hyperoxaluria induced urolithiasis in rats. Phytother Res 2010; 24:1250-1255.

71. Ashraf H, Zare S, Farnad N. The effect of aqueous extract of barberry fruit on liver damage in streptozotocin - induced diabetic rats. J Shahrekord Univ Med Sci 2013; 15:1-9.

72. SalehZadeh H, Iloun kashkooliR R, Najafi SS, Hosseini Asl MK, Hamedi A, Kalateh Sadati A. The effect of B‌erberis vulgaris extract on blood pressure and Weight of the wPatients suffered from Non-alcoholic fatty liver disease. jgbfnm  2013; 10: 21-27.

73.. Das S, Das MK, Mazumder PM, Das S, Basu SP. Cytotoxic activity of methanolic extract of Berberis aristata DC on colon cancer. Global J Pharmacol 2009; 3:137-140.

74. Motalleb G, Hanachi P, Fauziah O, Asmah R. Effect of Berberis vulgaris fruit extract on alpha-fetoprotein gene expression and chemical carcinogen metabolizing enzymes activities in hepatocarcinogenesis rats. Iran J Cancer Prev 2012; 1:33-42.

75. El-Merahbi R.  Berberis libanotica Ehrenb extract shows anti-neoplastic effects on prostate cancer stem/progenitor cells. PloS One 2014; 9:112453.

76. Khan M, Giessrigl B, Vonach C, Madlener S, Prinz S, Herbaceck I, et al. Berberine and a Berberis lycium extract inactivate Cdc25A and induce α-tubulin acetylation that correlate with HL-60 cell cycle inhibition and apoptosis. MUTAT RES-FUND MOL M 2010; 683:123-130.

77. Hanachi P, Othman F, Motalleb G. Effect of Berberis vulgaris aqueous extract on the apoptosis, sodium and potassium in hepatocarcinogenic rats. Iran J Basic Med Sci 2008; 11:62-69.

78. Fatehi M, Saleh TM, Fatehi-Hassanabad Z, Farrokhfal K, Jafarzadeh M, Davodi SA. A pharmacological study on Berberis vulgaris fruit extract. J Ethnopharmacol 2005; 102:46-52.

79. Alamgeer A, Akhtar MS, Jabeen Q, Akram M, Khan HU, Karim S, et al. Antihypertensive activity of aqueous-methanol extract of Berberis orthobotrys Bien Ex Aitch in rats. Trop J Pharm Res 2013; 12:393-399.

80. Mahdavi N, Joukar S, Najafipour H, Asadi-Shekaari M. The promising effect of barberry (Zereshk) extract against experimental pulmonary microvascular remodeling and hypertension: A comparison with sildenafil. Pharm Biol 2015; 54: 1-7.

81. Yeşilada E, Küpeli E. Berberis crataegina DC. Root exhibits potent anti-inflammatory, analgesic and febrifuge effects in mice and rats. J Ethnopharmacol 2002; 79 :237-248.

82. Joshi PV, Shirkhedkar AA, Prakash K, Maheshwari VL. Antidiarrheal activity, chemical and toxicity profile of Berberis aristata. Pharm Biol 2011; 49:94-100.

83. Shamkuwar P, Pawar D. Antidiarrhoeal and antispasmodic effect of Berberis aristata. Int J Pharm  Phytochem Res 2013; 5:24-26.

84. Bhutada P, Mundhada Y, Bansod K, Dixit P, Umathe S, Mundhada D. Anticonvulsant activity of berberine, an isoquinoline alkaloid in mice. Epilepsy Behav 2010; 18:207-210.

85. Singh M, Srivastava S, Rawat R. Antimicrobial activities of Indian Berberis species. Fitoterapia 2007; 78:574-576.

86. Changizi Ashtiyani S, Zarei A, Taheri S, Rezaei A, Golshan M, Ghafarzadegan R. A Comparative Study of Hypolipidemic Activities of the Extracts of Melissa officinalis and Berberis vulgaris in Rats. J Med Plants 2013; 3:38-47.

87. Razzaq FA, Khan RA, Feroz Z, Afroz S. Effect of Berberis aristata on lipid profile and coagulation parameters. African J Pharm Pharm 2011; 5:943-947.

88. Upwar N, Patel R, Waseem N, Mahobia NK. Hypoglycemic effect of methanolic extract of Berberis aristata DC stem on normal and streptozotocin induced diabetic rats. Int J Pharm Pharm Sci  2011; 3:222-224.

89. Ghuffar A, Ahmad T, Mushtaq MN. Antihyperlipidemic effectof Berberis orthobotrys in hyperlipidemic animal models. Bang J Pharmacol 2014; 9:377-382.

90. Ahmed M, Alamgeer A, Sharif T, Zabta C, Akbar A.  Effect of Berberis lycium Royle on lipid profile in alloxan induced diabetic rabbits. Ethnobotan Leaflets 2009; 13: 702-708.

91. Mustafaa KG, Ganai B, Akbar S, Dar M, Tantry M, Masood A. The extracts of Berberis lycium and diabetes mellitus in alloxan monohydrate induced diabetic rats. J Pharm Res 2011; 4:2570-2573.

92. Meliani N, Dib MEA, Allali H, Tabti B.  Hypoglycaemic effect of Berberis vulgaris L. in normal and streptozotocin-induced diabetic rats. Asian Pac J Trop Biomed 2011; 1:468-471.

93. Gulfraz M, Mehmood S, Ahmad A, Fatima N, Praveen Z, Williamson E. Comparison of the antidiabetic activity of Berberis lyceum root extract and berberine in alloxan‐induced diabetic rats. Phytother Res 2008; 22:1208-1212.

94. Ashraf H, Heidari R, Nejati V, Ilkhanipoor M. Aqueous extract of Berberis integerrima root improves renal dysfunction in streptozotocin induced diabetic rats. Avicenna J Phytomed 2012;.3:82-90.

95. Ashraf H, Heidari R, Nejati V, Ilkhanipoor M.  Effects of aqueous extract of berberis integerrima root on some physiological parameters in streptozotocin-induced diabetic rats. Iran J Pharm Res 2013; 12:425-434.

96. Ashraf H, Khaneshi F, Rafiee Raki F, Nejati V. Evaluation of aqueous extract of Berberis Integerrima root on the testis tissue and testosterone levels in stereptozotocine (stz) induced diabetic rats. Qom Univ Med Sci J 2013; 7: 28-35.

97. Fouladi RF.Aqueous extract of dried fruit of Berberis vulgaris L. in acne vulgaris, a clinical trial. J Diet Suppl 2012; 9:253-261.

98. Golzarand M, Ebrahimi-Mamaghani M, Arefhosseini S, Asgarzadeh AA. Effect of processed Berberis vulgaris in apple vinegar on blood pressure and inflammatory markers in type 2 diabetic patients. J Diabetes Metab Dis 2008; 7:3.

99. Moazezi Z,Qujeq D. Berberis fruit extract and biochemical parameters in patients with type II diabetes. Jundishapur J Nat Pharm Prod 2014; 9:2.

100. Shidfar F, Seyed Ebrahimi S, Hosseini S, Heydari I, Shidfar S, Hajhassani G.The effects of Berberis vulgaris fruit extract on serum lipoproteins, apoB, apoA-I, homocysteine, glycemic control and total antioxidant capacity in type 2 diabetic patients. Iran J Pharm Res 2012; 12: 643-652.

101. Derosa G, Bonaventura A, Bianchi L, Romano D, D'Angelo A, Fogari E, et al. Berberis aristata/Silybum marianum fixed combination on lipid profile and insulin secretion in dyslipidemic patients. Exp Opin Biol Ther 2013; 13:1495-1506.

102. Imanshahidi M, Hosseinzadeh H. Pharmaco-logical and therapeutic effects of Berberis vulgaris and its active constituent, berberine. Phytother Res 2008; 22:999-1012.

103. Baradaran A, Rafieian-kopaei M. Oxidative stress and hypertension: Possibility of hypertension therapy with antioxidants. J Res Med Sci 2014; 19:4.

104. Saleh Zadeh H, Iloun kashkooliR R, Najafi S, Hosseini Asl M, A. H, Kalateh Sadati A.The effect of B‌erberis vulgaris extract on blood pressure and weight of the patients suffered from Non-alcoholic Fatty Liver Disease. Journal of Research Development in Nursing & Midwifery (jgbfnm) 2013; 11:21-27.

105. Smith L,  Culvenor C. Plant sources of hepato-toxic pyrrolizidine alkaloids. J Nat Prod 1981; 44: 129-152.

106. Kulkarni S, Dhir A. Berberine: a plant alkaloid with therapeutic potential for central nervous system disorders. Phytother Res 2010; 24:317-324.

107. Javadzadeh SM,Fallah SR. Therapeutic applica-tion of different parts Berberis vulgaris. Int J Agric Crop Sci 2012; 4:404-408.

108. Torres R, Delle Monache F, Marini–Bettolo G. Biogenetic relationships between lignans and alkaloids in Berberis genus. Lignans and berbamine from Berberis chilensis. Planta Med 1979; 37: 37-44.