Efficacy of insulin targeted gene therapy for type 1 diabetes mellitus: A systematic review and meta-analysis of rodent studies
Moosa
Rahimi Ghiasi
Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
author
Hamed
Mohammadi
Food Security Research Center, School of Nutrition and Food Science, Isfahan University of Medical Sciences, Isfahan, Iran
author
Michael
Symonds
The Early Life Research Unit, Division of Child Health, Obstetrics and Gynaecology, and Nottingham Digestive Disease Centre and Biomedical Research Centre, School of Medicine, University of Nottingham, Nottingham, UK
author
Seyed Mohammad Bagher
Tabei
Department of Genetics & Maternal-Fetal Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
author
Ahmad Reza
Salehi
Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
author
Sima
Jafarpour
Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
author
Leila
Norouzi Barough
Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
author
Elnaz
Rahimi
Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
author
Zohreh
Amirkhani
Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
author
Maryam
Miraghajani
Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
author
Rasoul
Salehi
Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
author
text
article
2020
eng
Objective(s): Diabetes mellitus (DM) is a major worldwide public health challenge, for which gene therapy offers a potential therapeutic approach. To date, no systematic review or meta-analysis has been published in this area, so we examined all relevant published studies on rodents to elucidate the overall effects of gene therapy on bodyweight, intraperitoneal glucose tolerance test (IPGTT), fasting blood glucose, and insulin in animals with type 1 DM. Materials and Methods: The Cochrane Library, PubMed, Embase, ISI Web of Science, SCOPUS, and Google Scholar were systematically searched for potentially relevant studies. Mean±standard deviation (SD) was pooled using a random-effects model.Results: After the primary search, out of 528 studies identified, 16 studies were in concordance with predefined criteria and selected for the final assessment. Of these, 12 studies used viral manipulation, and 4 employed non-viral vectors for gene delivery. The meta-analysis showed gene therapy with a viral vector decreased mean IPGTT (-12.69 mmol/l, P<0.001), fasting blood glucose (-13.51 mmol/l, P<0.001), insulin (398.28 pmol/l, P<0.001), and bodyweight (24.22 g, P<0.001), whereas non-viral vectors reduced fasting glucose (-29.95 mmol/l, P<0.001) and elevated insulin (114.92 pmol/l, P<0.001).Conclusion: Gene therapy has favorable effects on alleviating type 1 DM related factors in diabetic rodents.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
4
no.
2020
406
415
https://ijbms.mums.ac.ir/article_14784_e5961d2a9643d732ee8fc250a5005e42.pdf
dx.doi.org/10.22038/ijbms.2020.39470.9359
Natural products as safeguards against monosodium glutamate-induced toxicity
Mahdi
Hajhasani
Department of Pharmaceutical Control, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
author
Vahid
Soheili
Department of Pharmaceutical Control, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
author
Mohammad Reza
Zirak
Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
author
Amir Hosein
Sahebkar
Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
author
Abolfazl
Shakeri
Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
author
text
article
2020
eng
Monosodium glutamate is a sodium salt of a nonessential amino acid, L-glutamic acid, which is widely used in food industry. Glutamate plays an important role in principal brain functions including formation and stabilization of synapses, memory, cognition, learning, as well as cellular metabolism. However, ingestion of foodstuffs rich in monosodium glutamate can result in the outbreak of several health disorders such as neurotoxicity, hepatotoxicity, obesity and diabetes. The usage of medicinal plants and their natural products as a therapy against MSG used in food industry has been suggested to be protective. Calendula officinalis, Curcuma longa, Green Tea, Ginkgo biloba and vitamins are some of the main natural products with protective effect against mentioned monosodium glutamate toxicity through different mechanisms. This review provides a summary on the toxicity of monosodium glutamate and the protective effects of natural products against monosodium glutamate -induced toxicity.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
4
no.
2020
416
430
https://ijbms.mums.ac.ir/article_14973_9bd2129f3ed6770371a2ed4048c9e375.pdf
dx.doi.org/10.22038/ijbms.2020.43060.10123
PuraMatrix hydrogel enhances the expression of motor neuron progenitor marker and improves adhesion and proliferation of motor neuron-like cells
Marzieh
Darvishi
Department of Anatomy, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran
author
Hatef
Ghasemi Hamidabadi
Department of Anatomy & Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
author
Sajad
Sahab Negah
Neuroscience Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
author
Ardeshir
Moayeri
Department of Anatomy, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran
author
Taki
Tiraihi
Department of Anatomical Sciences, Faculty of Medical Sciences, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
author
Javad
Mirnajafi-Zadeh
Department of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
author
Ali
Jahanbazi Jahan-Abad
Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran
author
Amir
Shojaei
Department of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
author
text
article
2020
eng
Objective(s): Cell therapy has provided clinical applications to the treatment of motor neuron diseases. The current obstacle in stem cell therapy is to direct differentiation of stem cells into neurons in the neurodegenerative disorders. Biomaterial scaffolds can improve cell differentiation and are widely used in translational medicine and tissue engineering. The aim of this study was to compare the efficiency of two-dimensional with a three-dimensional culture system in their ability to generate functional motor neuron-like cells from adipose-derived stem cells. Materials and Methods: We compared motor neuron-like cells derived from rat adipose tissue in differentiation, adhesion, proliferation, and functional properties on two-dimensional with three-dimensional culture systems. Neural differentiation was analyzed by immunocytochemistry for immature (Islet1) and mature (HB9, ChAT, and synaptophysin) motor neuron markers. Results: Our results indicated that the three-dimensional environment exhibited an increase in the number of Islet1. In contrast, two-dimensional culture system resulted in more homeobox gene (HB9), Choline Acetyltransferase (ChAT), and synaptophysin positive cells. The results of this investigation showed that proliferation and adhesion of motor neuron-like cells significantly increased in three-dimensional compared with two-dimensional environments. Conclusion: The findings of this study suggested that three-dimension may create a proliferative niche for motor neuron-like cells. Overall, this study strengthens the idea that three-dimensional culture may mimic neural stem cell environment for neural tissue regeneration.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
4
no.
2020
431
438
https://ijbms.mums.ac.ir/article_14926_7ba8febb69eca2b02971325e59cecd8f.pdf
dx.doi.org/10.22038/ijbms.2020.39797.9434
Paeoniflorin regulates the hypothalamic-pituitary-adrenal axis negative feedback in a rat model of post-traumatic stress disorder
Jie
Chen
The Research Centre of Basic Integrative Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
author
Weiqiong
Ye
The Research Centre of Basic Integrative Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
author
Ling
Li
The Research Centre of Basic Integrative Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
author
Junfang
Su
Department of Pathology and Pathophysiology, School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China
author
Yunling
Huang
The Research Centre of Basic Integrative Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
author
Lingyun
Liu
The Research Centre of Basic Integrative Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
author
Lili
Wu
The Research Centre of Basic Integrative Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
author
Can
Yan
The Research Centre of Basic Integrative Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
author
text
article
2020
eng
Objective(s): To investigate the effects of paeoniflorin (PEF) on the hypothalamic-pituitary-adrenal (HPA) axis feedback function of post-traumatic stress disorder (PTSD). cSingle-prolonged stress (SPS) was used to establish a PTSD-like rat model. The contents of plasma corticosterone (CORT), adrenocorticotropin hormone (ACTH) and corticotropin-releasing hormone (CRH) were measured by ELISA. Glucocorticoid receptor (GR), mineralocorticoid receptor (MR), adrenocorticotropic hormone-releasing factor I receptor (CRF1R), and adrenocorticotropic hormone-releasing factor II receptor (CRF2R) in the hippocampus and amygdala were measured by RT-PCR and immunohistochemistry.Results: The results showed that on day 8 after SPS, model rats showed enhanced HPA axis negative feedback lasting to day 29. On day 29, plasma CORT levels increased in model rats, while plasma CRH levels had no significant difference on days 8, 22, and 29. The expression of GR and MR of model rats significantly increased in the hippocampus, while the expression of GR, MR, and CRF1R significantly decreased in the amygdala. After 14 days of continuous administration of PEF, the enhanced negative feedback was inhibited, and the plasma CORT level significantly reduced after 21 days of administration. Moreover, PEF could significantly decrease the expression of GR and MR in the hippocampus, and increase the expression of GR, MR, and CRF1R significantly in the amygdala. Conclusion: PEF could regulate HPA axis dysfunction in a rat model of PTSD, which may be related to regulating expression of GR and MR in the hippocampus and amygdala and regulating expression of CRF1R in the amygdala.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
4
no.
2020
439
448
https://ijbms.mums.ac.ir/article_14781_efb7a67396e32ddde4bac1d0d347e5c7.pdf
dx.doi.org/10.22038/ijbms.2020.41214.9738
PHA stimulation may be useful for FDXR gene expression-based biodosimetry
Habibeh
Vosoughi
Department of Medical Physics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Hosein
Azimian
Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
author
Sara
Khademi
Department of Radiology Technology, School of Paramedical Sciences, Mashhad University of Medical Sciences, Mashhad, Iran
author
Abdul-Rahim
Rezaei
Immunology Research Center, Inflammation and Inflammatory Diseases Division, Mashhad University of Medical Sciences, Mashhad, Iran
author
Maryam
Najafi Amiri
Department of Medical Physics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Fereshteh
vaziri-nezamdoost
Department of Medical Physics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Mohammad-taghi
Bahreyni-Toossi
Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
author
text
article
2020
eng
Objective(s): Nowadays, ionizing radiation (IR) has a significant contribution to the diagnostic and therapeutic medicine, and following that, health risks to individuals through unexpected exposure is greatly increased. Therefore, biological and molecular technology for estimation of dose (biodosimetry) is taken into consideration. In biodosimetry methods stimulation of cells to proliferation is routine to achieve more sensitivity of techniques. However, this concept has recently been challenged by new molecular methods such as gene expression analysis. This study aims to investigate the stimulation effects on gene expression biodosimetry.Materials and Methods: The blood samples were taken from15 patients who were irradiated by TC-99 MIBI, before radiopharmaceutical injection and 24 hr after injection. Lymphocytes were extracted immediately and activated by (phytohemagglutinin) PHA for 24 hr and XPA and FDXR expression levels were investigated by employing relative quantitative Real-Time PCR.Results: The results of this study show a significant increase in the FDXR expression level and a significant decrease in the XPA after stimulation of irradiated lymphocytes. Interestingly, a significant increasing trend in the FDXR expression level (at 0.05 significance level) following cell stimulation to the division was observed.Conclusion: Our results suggest that the PHA activation role in gene expression-based biodosimetry is strongly depended on the target genes and the relevant protein pathways. Finally, cell stimulation looks to be useful for some specific genes, such as FDXR, due to the increasing trend in expression and improvement of sensitivity of gene expression-based biodosimetry method.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
4
no.
2020
449
453
https://ijbms.mums.ac.ir/article_14872_738d21378da0fc5651c13da6b16c2289.pdf
dx.doi.org/10.22038/ijbms.2020.42350.9997
Biochemical and computational study of an alginate lyase produced by Pseudomonas aeruginosa strain S21
Firoozeh
Piroozmand
Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
author
Parinaz
Ghadam
Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
author
Mahboobe
Zarrabi
Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
author
Ahya
Abdi Ali
Department of Microbiology, Faculty of Biological sciences, Alzahra University, Tehran, Iran
author
text
article
2020
eng
Objective(s): Alginates play a key role in mucoid Pseudomonas aeruginosa colonization, biofilm formation, and driving out of cationic antibiotics. P. aeruginosa alginate lyase (AlgL) is a periplasmic enzyme that is necessary for alginate synthesis and secretion. It also has a role in depolymerization of alginates. Using AlgLs in cystic fibrosis patients along with antibiotics enhances bacterial killing and host healing. In this study, we investigated the different biochemical properties of a newly isolated AlgL from P. aeruginosa S21 to complete the databank of AlgLsMaterials and Methods: The enzyme was extracted from the periplasmic space of the bacteria by the heat shock method. Using the TBA method, the enzyme activity and biochemical properties were assessed. The mutability of P. aeruginosa S21 AlgL to increase its thermal stability was investigated. The most favorable mutations were studied computationally. The molecular dynamics simulation (MDS) package GROMACS was used for determining the effect of S34R mutation on enzyme’s thermal stability.Results: Data showed that this enzyme has the best activity at 37 °C and pH 7.5 and it can degrade mannuronate blocks, guluronate blocks, and sodium alginate. After 7 hr at 80 °C, 45% of the enzyme activity was retained. This enzyme needed 15 min to completely degrade accessible sodium alginate. Tris buffer, pH 8.5 and Britton-Robinson buffer, pH 7.0 were the preferable buffers for the enzyme activity. MDS of native and mutated enzymes showed desirable results.Conclusion: P. aeruginosa S21 AlgL can be used in medical and industrial applications to degrade alginates.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
4
no.
2020
454
460
https://ijbms.mums.ac.ir/article_14788_098ee43aa148f3558057f72d42b539c8.pdf
dx.doi.org/10.22038/ijbms.2020.37277.8874
Monascin ameliorate inflammation in the lipopolysaccharide-induced BV-2 microglial cells via suppressing the NF-κB/p65 pathway
Yong-Xiang
Shi
Department of Orthopedics Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, 88 Jiefang Road, Hangzhou, 310009, Zhejiang, People’s Republic of China
author
Wei-Shan
Chen
Department of Orthopedics Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, 88 Jiefang Road, Hangzhou, 310009, Zhejiang, People’s Republic of China
author
text
article
2020
eng
Objective(s): The pathophysiology of neurodegenerative diseases is complicated, in which inflammatory reactions play a vital role. Microglia cells activation, an essential process of neuroinflammation, can produce neurotoxic molecules and neurotrophic factors, which aggravate inflammation and neuronal injury. Monascin, a major component of red yeast rice, is an azaphilonoid pigment with potential anti-inflammatory effects; however, the effects in central nervous system have not been evaluated. Our goal in this project was to explore the therapeutic effect and the underlying mechanism of Monascin, which may be via anti-inflammatory action.Materials and Methods: We used lipopolysaccharide to induce BV-2 microglial cells in order to form an inflammation model in vitro. The anti-inflammatory effects of Monascin were measured by enzyme-linked immunosorbent assay (ELISA), real time-polymerase chain reaction (RT-PCR), Western Blot and Immunofluorescent staining. Results: Our data indicated that inflammatory cytokines including interleukin-1β (IL-1β), IL-6, tumor necrosis factor-alpha (TNF-α) and nitric oxide were suppressed by Monascin treatment. Furthermore, the related pro-inflammatory genes were inhibited consistent with the results of ELISA assay. Western blotting results showed that the phosphorylation of nuclear factor kappa B (NF-κB/p65) was reduced by Monascin treatment may be through suppressing the activation of IκB. Furthermore, immunofluorescence staining showed that the translocation of NF-κB/p65 to the cellular nuclear was blockaded after Monascin treatment.Conclusion: Taken together, Monascin exerts anti-inflammatory effect and suppressed microglia activation, which suggested its potential therapeutic effect for inflammation-related diseases.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
4
no.
2020
461
468
https://ijbms.mums.ac.ir/article_14685_6217fd6a31669c9f417ab07a95f3222b.pdf
dx.doi.org/10.22038/ijbms.2020.41045.9702
Apoptosis induction and proliferation inhibition by silibinin encapsulated in nanoparticles in MIA PaCa-2 cancer cells and deregulation of some miRNAs
Fatemeh
Khakinezhad Tehrani
Department of Biology, Faculty of Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran
author
Najmeh
Ranji
Department of Biology, Faculty of Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran
author
Fatemeh
Kouhkan
Stem cell Technology Research Center, Tehran, Iran
author
Simzar
Hosseinzadeh
Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
author
text
article
2020
eng
Objective(s): Silibinin, as an herbal compound, has anti-cancer activity. Because of low solubility of silibinin in water and body fluids, it was encapsulated in polymersome nanoparticles and its effects were evaluated on pancreatic cancer cells and cancer stem cells.Materials and Methods: MIA PaCa-2 pancreatic cancer cells were treated with different doses of silibinin encapsulated in polymersome nanoparticles (SPNs). Stemness of MIA PaCa-2 cells was evaluated by hanging drop technique and CD133, CD24, and CD44 staining. The effects of SPNs on cell cycle, apoptosis and the expression of several genes and miRNAs were investigated. Results: IC50 of SPNs was determined to be 40 µg/ml after 24 hr. Our analysis showed that >98% of MIA PaCa-2 cells expressed three stem cell markers. FACS analysis showed a decrease in these markers in SPNs-treated cells. PI/AnnexinV staining revealed that 40 µg/ml and 50 µg/ml of SPNs increased apoptosis up to ~40% and >80% of treated cells, respectively. Upregulation of miR-34a, miR-126, and miR-let7b and downregulation of miR-155, miR-222 and miR-21 was observed in SPNs-treated cells. In addition, downregulation of some genes involved in proliferation or migration such as AKT3, MASPINE, and SERPINEA12, and upregulation of apoptotic genes were observed in treated cells.Conclusion: Our results suggested that SPNs induced apoptosis and inhibited migration and proliferation in pancreatic cells and cancer stem cells through suppression of some onco-miRs and induction of some tumor suppressive miRs, as well as their targets.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
4
no.
2020
469
482
https://ijbms.mums.ac.ir/article_14686_343176cf0f369a7764d9cc667ebf2df4.pdf
dx.doi.org/10.22038/ijbms.2020.39427.9349
Effect of treadmill exercise on catalepsy and the expression of the BDNF gene in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine male NMRI mice
Neda
Nikokalam Nazif
Department of Biology, Faculty of Biological Sciences, North-Tehran Branch, Islamic Azad University, Tehran, Iran
author
Maryam
Khosravi
Department of Biology, Faculty of Biological Sciences, North-Tehran Branch, Islamic Azad University, Tehran, Iran
author
Ramesh
Ahmadi
Department of Physiology, Qom Branch, Islamic Azad University, Qom, Iran
author
Maryam
Bananej
Department of Biology, Faculty of Biological Sciences, North-Tehran Branch, Islamic Azad University, Tehran, Iran
author
Ahmad
Majd
Department of Biology, Faculty of Biological Sciences, North-Tehran Branch, Islamic Azad University, Tehran, Iran
author
text
article
2020
eng
Objective(s): It is known that treadmill exercise has beneficial effects on the nervous system. The brain-derived neurotrophic factor (BDNF) plays a role in such effects. This study aimed at investigating effects of intermittent treadmill exercise-induced behavioral, histology, and immunohistochemistry (H&E, TH) measurement of brain interleukin-10 (IL-10) in a mice male model of Parkinson’s disease (PD), which is induced by intraperitoneal injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), as well as the role of BDNF gene in exercise effects. Materials and Methods: The animals were divided into Control (C), Saline (S), Parkinson (P), Exercise (E), and Parkinson + Exercise (PE) groups. Bar test was performed for the 21-day protocol with 5 days a week treadmill exercises. In this regard, brains were removed from the skull for H&E, TH, IL-10, and the expression of the BDNF gene using the MPTP male mice PD model.Results: MPTP reduced the number of DA neurons in the substantia nigra (SNpc), whereas daily exercise administration on 1st, 7th, 14th, and 21st days significantly reduced the catalepsy duration induced by MPTP. The results of H&E and TH studies showed that MPTP significantly reduced the number of TH+ neurons in the SNpc compared with those of the control mice. The MPTP caused a marked decrease in basal protein levels of IL-10 in SNpc and corpus striatum in the Parkinson (P) group as compared with controls. Treatment with Exercise (E) group had the most BDNF expression (3.71), and the Parkinson (P) group also had the least BDNF expression (0.18) relative to controls. Conclusion: The treadmill exercise having neuroprotective effects in SNpc and corpus striatum has improved MPTP associated with motor deficits. It is considered as a non-pharmacological tool for the management of PD.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
4
no.
2020
483
493
https://ijbms.mums.ac.ir/article_14694_e592ce732dd23e200b224d5063b7da33.pdf
dx.doi.org/10.22038/ijbms.2020.37707.8960
Hydrogen-rich saline ameliorates hippocampal neuron apoptosis through up-regulating the expression of cystathionine β-synthase (CBS) after cerebral ischemia- reperfusion in rats
Haiming
Cong
Department of Rehabilitation Medicine, Weihai Central Hospital, Wendeng 264400, China
author
Qiu-ping
Gao
Department of Emergency, Ji Nan Zhang Qiu District Hospital of Traditional Chinese Medicine, Zhang Qiu 250200, China
author
Guo-qiang
Song
Department of Neurosurgery, Ji Nan Zhang Qiu District Hospital of Traditional Chinese Medicine, Zhang Qiu 250200, China
author
Yin-gxin
Ye
Department of Neurology, Ji Nan Zhang Qiu District Hospital of TraditionalChinese Medicine, Zhang Qiu 250200, China
author
Xiao-li
Li
Department of Critical Care Medicine, Yu Huang Ding Hospital, Qingdao University, Yantai, P. R. China
author
Lian-shuang
Zhang
Department of Histology and Embryology, Binzhou Medical University, Yantai, P. R. China
author
Xi-feng
Wang
Department of Critical Care Medicine, Yu Huang Ding Hospital, Qingdao University, Yantai, P. R. China
author
text
article
2020
eng
Objective(s): This study aimed to evaluate the potential role of hydrogen in rats after cerebral ischemic/reperfusion (I/R) injury. Materials and Methods: The experimental samples were composed of sham group, model group of rats that received middle cerebral artery occlusion (MCAO) for 2 hr followed by reperfusion for 24 hr, and the hydrogen saline group treated by hydrogen-rich saline (1 ml/kg) after MCAO. Hydrogen sulfide (H2S), S100-βprotein (S100-β), and neuron-specific enolase (NSE) levels were measured; the levels of malondialdehyde (MDA), reactive oxygen species (ROS), and superoxide dismutase (SOD) were detected; the histologic structure and apoptotic cells of hippocampus were observed; the expressions of cystathionine β-synthase (CBS), nuclear factor erythroid 2-related factor 2 (Nrf2), and hemeoxygenase-1 (HO-1) were measured. Statistical analyses were performed using one-way analysis of variance (ANOVA) followed by Fisher’s least significant difference (LSD) test.Results: Our results showed that hydrogen up-regulated H2S levels via promoting the expression of CBS in the hippocampus, and its treatment alleviated oxidative stress via activating the expression of Nrf2 and HO-1, and then cell apoptosis reduced, furthermore, brain function improved by down-regulating the levels of S100-βand NSE. Conclusion: This study showed that hydrogen-rich saline ameliorates cell injury through up-regulating the expression of CBS in the hippocampus after cerebral ischemia reperfusion (I/R) in rats, this provides new experimental evidence for the treatment of stroke with hydrogen saline.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
4
no.
2020
494
499
https://ijbms.mums.ac.ir/article_14868_7545272bf81406bc7bff9bc5e1958258.pdf
dx.doi.org/10.22038/ijbms.2020.41751.9857
High proviral load of human T cell lymphotropic virus type-1 facilitates coronary artery diseases
Farnaz
Mozayeni
Allergy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Seyed Abdolrahim
Rezaee
Immunology Research Center, Inflammation and Inflammatory Disease Division, Mashhad University of Medical Sciences, Mashhad, Iran
author
Farahnaz
Jabbari Azad
Allergy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Mahmoud
Shabestari
Department of Cardiology, Emam Reza Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Reza
Faridhosseini
Allergy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Houshang
Rafatpanah
Immunology Research Center, Inflammation and Inflammatory Disease Division, Mashhad University of Medical Sciences, Mashhad, Iran
author
Hadis
Yousefzadeh
Immunology Research Center, Bu Ali Research Institute, Student Research Committee, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Yousef Ali
Garivani
Department of Cardiology, Emam Reza Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Lida
Jarahi
Addiction Research Center, Mashhad University of Medical Sciences, Faculty of Medicine, Mashhad, Iran
author
Narges
Valizadeh
Immunology Research Center, Inflammation and Inflammatory Disease Division, Mashhad University of Medical Sciences, Mashhad, Iran
author
Faezah
Sabet
Immunology Research Center, Inflammation and Inflammatory Disease Division, Mashhad University of Medical Sciences, Mashhad, Iran
author
Sharare
Moshirahmadi
Department of Cardiology, Emam Reza Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Fatemeh Sadat
Mohammadi
Allergy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
Mohammad
Shabestari
Department of Cardiology, Emam Reza Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
text
article
2020
eng
Objective(s): Coronary artery disease (CAD) is known as a life threatening disease, worldwide. In this study the role of HTLV-1 infection was evaluated on cardiac involvement in an endemic region of northeastern Iran.Materials and Methods: Serologic and molecular tests for HTLV-1 infection were carried out in subjects who had coronary angiography. A real-time PCR, TaqMan method, to quantify HTLV-1 proviral load (PVL), and routine hematological and biochemical tests were performed for study subjects. Results: Twenty nine patients were HTLV-1+CAD+ and 13 cases were HTLV-1+CAD-. Although, there were no significant differences for risk factors like FBS, HDL, triglyceride, systolic and diastolic blood pressure (Cbp, Dbp), waist circumference (WC), hip circumference (WL), cholesterol (P=0.003), and LDL (P=0.007) levels, and monocyte count (P=0.05) had meaningful differences. The mean HTLV-1 PVL in HTLV-1+CAD+ subjects was 992.62±120 which was higher compared with HTLV-1+CAD- group (406.54±302 copies/104 PBMCs). Moreover, HTLV-1 PVL in males (833±108) was lower compared with females (1218±141 copies/104 PBMCs) (P=0.05). Patients with HTLV-1-PVL of more than 500 copies/104 had more diffused atherosclerosis plaque than patients with less than 500 (OR=6.87, 95% CI=1.34-35.05; P=0.016). Furthermore, patients with diffused coronary atherosclerosis had significantly higher levels of HTLV-1 PVL than patients with middle, proximal, and normal location of coronary sclerotic lesions (P<0.05). Conclusion: Taken together, in endemic area, HTLV-1 infection, more likely is a facilitating factor for heart complications and the high HTLV-1 PVL might affect CAD manifestations.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
4
no.
2020
500
506
https://ijbms.mums.ac.ir/article_14627_9dd7bb9cfba7258a993b792c4cc777dd.pdf
dx.doi.org/10.22038/ijbms.2020.36317.8649
Induction of humoral immune responses and inhibition of metastasis in mice by a VEGF peptide-based vaccine
Faezeh
Soltanpour Gharibdousti
Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
author
Banafsheh
Fazeli Delshad
Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
author
Reza
Falak
Immunology Research Center, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
author
Nasrin
Shayanfar
Department of Pathology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
author
Mazdak
Ganjalikhani Hakemi
Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
author
Alireza
Andalib
Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
author
Gholamali
Kardar
Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran
author
text
article
2020
eng
Objective(s): Blocking of vascular endothelial growth factor (VEGF) plays a pivotal role in inhibition of metastasis and is a target for development of anti-angiogenic agents. In this study, a peptide-based vaccine was designed and its potential for induction of humoral immune responses, generation of neutralizing antibodies, inhibition of tumor growth and metastasis was determined.Materials and Methods: With online bioinformatics tools, a fragment of the VEGF-A was selected for a peptide-based vaccine. To enhance its antigenicity, the peptide was conjugated with Keyhole limpet hemocyanin and used to immunize mice. Then, the polyclonal anti-VEGF antibody titer was measured and its effect on proliferation of HUVEC cell line was investigated by MTT assay. Finally, we checked the effect of the peptide on tumor growth, metastasis, and survival rates in a mouse model of cancer.Results: The bioinformatics analysis of the selected region confirmed dis-similarity of the peptide with any other human protein and its acceptable antigenicity to stimulate a tumor-specific humoral response. Anti-VEGF antibody titers were significantly greater in vaccinated mice than in controls. IgG antibody from mice immunized with recombinant VEGF-A inhibited HUVEC proliferation (P<0.0001). Tumors in vaccinated mice were significantly smaller than those in controls. Moreover, metastasis was reduced and survival rates increased in the vaccinated group.Conclusion: Production of high-titer antibody against the peptide vaccine indicated that the peptide has the potency to be used as a peptide-based vaccine for humoral inhibition of tumor growth and metastasis. The efficacy of the peptide should be further tested in primate models.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
4
no.
2020
507
514
https://ijbms.mums.ac.ir/article_14629_76b0ec041e5e36d408ab32072b341fa9.pdf
dx.doi.org/10.22038/ijbms.2020.38508.9141
Involvement of Mfn2, Bcl2/Bax signaling and mitochondrial viability in the potential protective effect of Royal jelly against mitochondria-mediated ovarian apoptosis by cisplatin in rats
khalid
Hashem
Department of Biochemistry, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt
author
Asmaa
Elkelawy
Department of Pharmacology, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt
author
Saber
Abd-Allah
Department of Theriogenology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt
author
Nermeen
Helmy
Department of Physiology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt
author
text
article
2020
eng
Objective(s): The current study aimed to assess cisplatin-mediated ovarian apoptosis in a rat model by Royal jelly (RJ). Materials and Methods: Thirty female adult albino rats (180-200 g) were divided into three groups (n=10): saline (0.9% NaCl, IP) was given to the control group, the cisplatin group: received (5 mg/kg/once a week IP) for 5 successive weeks, the RJ+Cis. group: received RJ (100 mg/kg/ day PO daily), and Cisplatin (5 mg/kg/once per week IP) for 5 successive weeks. At the end of the experiment, rats were sacrificed and their ovaries were isolated and used for biochemical analysis, molecular investigations and morphometric assessment as well as histological study. Moreover, blood samples were collected for determination of follicle-stimulating hormone (FSH), luteinizing hormone (LH), Estradiol, progesterone and anti-mullerian hormone (AMH).Results: The current study clarified that RJ given to rats prior to cisplatin significantly increased the ovarian and uterine weights, in addition to follicular count at P˂0.05 compared to rats injected only with cisplatin. Moreover, it restored normal ovarian histological structure with a concurrent reduction in FSH, and LH levels, and increased AMH and ovarian hormone concentrations at P˂0.05 compared to cisplatin group. Also, RJ decreased the ovarian antioxidant/oxidative imbalance harmonized with significant suppression of inducible nitric oxide synthase and increase of quinone oxidoreductase 1 mRNA expression at P˂0.05 compared to cisplatin group.Conclusion: We concluded that RJ could alleviate mitochondrial-induced ovarian apoptosis caused by cisplatin via increasing anti-apoptotic Bcl2, and diminishing pro-apoptotic Bax with a concomitant increase of Mfn2 mRNA and protein expressions.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
4
no.
2020
515
526
https://ijbms.mums.ac.ir/article_14783_9eb0f4ada29e767ac4dd1234224d206e.pdf
dx.doi.org/10.22038/ijbms.2020.40401.9563
The effect of lycopene in egg shell membrane guidance channel on sciatic nerve regeneration in rats
Gholam Hossein
Farjah
Neurophysiology Research Center, Department of Anatomy, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran
author
Samad
Mohammadzadeh
Department of Anatomy, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran
author
Masoumeh
Zirak Javanmard
Department of Anatomy, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran
author
text
article
2020
eng
Objective(s): Peripheral nerves are commonly damaged. Although the nerve autograft still subsists the gold standard in surgery of nerve injury gaps, it has severe detriment. The egg shell membrane (ESM) is a natural material, which has a great potential in practice. The aim of this survey was to appraise the effect of lycopene and the ESM nerve guidance channel on sciatic nerve regeneration.Materials and Methods: In this study, 32 male rats were randomized into four groups: sham surgery, autograft, ESM+ dimethyl sulfoxide (DMSO), and ESM+lycopene. One centimeter of sciatic nerve was removed, and the gap was grafted by ESM channel or autograft. The sciatic function index (SFI) was evaluated at days 7, 21, 30, 49, 60 and 90 after surgery. Nerve regeneration and gastroknemius muscle fibers were evaluated at days 30 and 90 after surgery by withdrawal reflex latency (WRL), histology and immunohistology assessments.Results: At 49, 60 and 90 days after surgery, the mean SFI in ESM+lycopene group was significantly greater than ESM+DMSO group (P<0.05). On day 90, the mean muscle fiber diameters and the mean number of myelinated axons in ESM+lycopene and autograft groups were significantly greater than ESM+DMSO group (P<0.05). In addition, the mean WRL was significantly lower in ESM+lycopene group than in the ESM+DMSO group 90 days after surgery (P<0.05).Conclusion: The results of this study show that the affirmative effects of ESM+lycopene may be beneficial for treating peripheral nerve damages.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
4
no.
2020
527
533
https://ijbms.mums.ac.ir/article_14869_689ccbfea6fd84ca015ae4339184681f.pdf
dx.doi.org/10.22038/ijbms.2020.40228.9525
Analysis of the active fraction of Iranian Naja naja oxiana snake venom on the metabolite profiles of the malaria parasite by 1HNMR in vitro
Fatemeh
Hajialaiani
Medical Parasitology Department, School of Medicine -International Campus, Iran University of Medical Sciences, Tehran, Iran
author
Taher
Elmi
Parasitology and Mycology Department, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
author
Maryam
Mohammadi
Biochemistry Department, Pasteur Institute of Iran, Pasteur Avenue, Tehran. Iran
author
Delavr
Shahbazzadeh
Laboratory of Venom and Bio Therapeutics Molecules, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
author
Fatemeh
Ghafarifar
Department of Parasitology, Faculty of Medical Sciences, Tarbiat modares University Tehran, Iran
author
Abolhossein
Dalimi Asl
Department of Parasitology, Faculty of Medical Sciences, Tarbiat modares University Tehran, Iran
author
Mohammad
Arjmand
Biochemistry Department, Pasteur Institute of Iran, Pasteur Avenue, Tehran. Iran
author
Fatemeh
Tabatabaie
Parasitology and Mycology Department, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
author
Zahra
Zamani
Biochemistry Department, Pasteur Institute of Iran, Pasteur Avenue, Tehran. Iran
author
text
article
2020
eng
Objective(s): Malaria is an important parasitic disease with high morbidity and mortality in tropical areas. Resistance to most antimalarial drugs has encouraged the development of new drugs including natural products. Venom is a complex mixture of active pharmaceutical ingredients. The purpose of this study was to investigate the antimalarial activity of purified fractions of Naja naja oxiana. Materials and Methods: Lyophilized venom was purified with a Sephacryl S-200 HR column and the fractions lyophilized and inhibitory concentration 50% against Plasmodium falciparum 3D7 in vitro obtained. The 4th fraction was run on a Mono Q column, and activity against P. falciparum was detected by lactate dehydrogenase assay and purity by SDS PAGE. Large scale culture of the parasite was carried out with and without the active fraction on the ring stage for 48 hr. The parasites were collected and lyophilized and analyzed by 1HNMR. Chemometrics studies were performed using MATLAB, differentiating metabolites were identified by Human Metabolic Database, and metabolic pathways by the Metaboanalyst online package. Results: The active fraction from the ion exchange column had a 50% inhibitory concentration of 0.026 µg/ml on P. falciparum in vitro (P<0.001) with molecular weight of 63 kDa by SDS-PAGE and no hemolytic activity. Metabolomics studies on the two groups with and without the fraction identified 5 differentiating metabolites and a number of related pathways.Conclusion: The metabolites were succinic acid, l-glutamic acid, pyruvic acid, cholesterol, and NAD. The changes in the Krebs cycle and metabolism pathways of nicotinamide and pyruvate were noticeable.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
4
no.
2020
534
543
https://ijbms.mums.ac.ir/article_14975_e876d7961d833735d8ea2820a785a2f3.pdf
dx.doi.org/10.22038/ijbms.2020.39386.9344
Differential gene expression by lithium chloride induction of adipose-derived stem cells into neural phenotype cells
Samaneh
Farrokhfar
Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
author
Taki
Tiraihi
Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, P.O.BOX.14115-331 Tehran, Iran
author
Mansoureh
Movahedin
Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, P.O.BOX.14115-331 Tehran, Iran
author
Hossein
Azizi
Department of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, P.O.BOX.14115-331 Tehran, Iran
author
text
article
2020
eng
Objective(s): Adipose-derived stem cells (ADSCs), with suitable and easy access, are multipotential cells that have the ability for differentiation into other mesodermal and transdifferentiate into neural phenotype cells. In this study, Lithium chloride (LiCl) was used for in vitro transdifferentiation of rat ADSCs into neuron-like cells (NLCs).Materials and Methods: ADSCs were isolated from the rats’ perinephric region using Dulbecco΄s Modified Eagle΄s Medium (DMEM) with Fetal Bovine Serum (FBS), cultured for 3 passages, characterized by flowcytometry and differentiation into adipogenic and osteogenic phenotypes. The ADSCs were exposed to 0.1, 0.5, 1, 1.5, 2, 5, and 10 millimolar (mM) LiCl without serum for 24 hr. The optimum dose of LiCl was selected according the maximum viability of cells. The expression of neurofilament light chain (NfL), neurofilament high chain (NfH), and nestin was evaluated by immunocytochemistry. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to evaluate the amount of synaptophysin, neurogenin-1, neuroD1, NfL, NfH, and nestin genes’ expression in ADSCs and NLCs.Results: The optimum dose of LiCl was 1 mM in 24 hr. The transdifferentiated ADSCs showed cytoplasmic extension with synapse-like formation. Synaptophysin, neurogenin-1, neuroD1, NfL, NfH, and nestin genes were significantly expressed more in NLCs than in ADSCs.Conclusion: LiCl can induce ADSCs into neural phenotype cells with higher expression of neural and neuronal genes.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
4
no.
2020
544
550
https://ijbms.mums.ac.ir/article_14974_3561b8def012f06d100cedc52a199c72.pdf
dx.doi.org/10.22038/ijbms.2020.41582.9820
Preparation and in vivo evaluation of nanoliposomes containing vancomycin after intravitreal injection in albino rabbits
Majid
Abrishami
Eye Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
author
Mehrdad
Motamed Shariati
Retina Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
author
Bizhan
Malaekeh-Nikouei
Nanotechnology Research Center, Institute of Pharmaceutical Technology, Mashhad University of Medical Sciences, Mashhad, Iran
author
Amineh
Sadat Tajani
Department of Pharmaceutical Control, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
author
Asma
Mahmoudi
Nanotechnology Research Center, Institute of Pharmaceutical Technology, Mashhad University of Medical Sciences, Mashhad, Iran
author
Mojtaba
Abrishami
Eye Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
author
Bahman
Khameneh
Department of Pharmaceutical Control, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
author
text
article
2020
eng
Objective(s): The in vivo efficacy of nanoliposomal formulation of vancomycin against methicillin-resistant Staphylococcus aureus (MRSA) assessed.Materials and Methods: Nanoliposomal formulations were prepared and characterized. The in vivo study was carried out on rabbits which received liquid culture medium containing MRSA under anesthesia. After 48 hr, the eyes treated with the liposomal and free form of vancomycin. The rabbits were euthanized at predesignate intervals at 12, 24, 48, 96, 144 hr intervals injection. The antibacterial activity of different vancomycin formulations was assayed by the time killing method. Results: The zeta potential, mean sizes and encapsulation efficacy of liposomal vancomycin were 29.7 mV, 381.93±30.13 nm and 47%, respectively. The results of time–killing studies indicated that the liposomal formula was more effective than the free form of vancomycin.Conclusion: The results of this study revealed that liposomal vancomycin formulation is a powerful nano-antibacterial agent to combat infectious endophthalmitis.
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
2008-3866
23
v.
4
no.
2020
551
555
https://ijbms.mums.ac.ir/article_14967_afaa6e979f6700f48a6f30ae7fc78c7c.pdf
dx.doi.org/10.22038/ijbms.2020.43447.10205