@article { author = {Ghasemi, Sepideh and Aligholi, Hadi and Koulivand, Pir Hossain and Jafaraian, Maryam and Hosseini Ravandi, Hassan and Khaleghi Ghadiri, Maryam and Gorji, Ali}, title = {Generation of motor neurons from human amygdala-derived neural stem-like cells}, journal = {Iranian Journal of Basic Medical Sciences}, volume = {21}, number = {11}, pages = {1155-1160}, year = {2018}, publisher = {Mashhad University of Medical Sciences}, issn = {2008-3866}, eissn = {2008-3874}, doi = {10.22038/ijbms.2018.29587.7146}, abstract = {Objective(s): Among several cell sources, adult human neural stem/progenitor cells (hNS/PCs) have been considered outstanding cells for performing mechanistic studies in in vitro and in vivo models of neurological disorders as well as for potential utility in cell-based therapeutic approaches. Previous studies addressed the isolation and culture of hNS/PCs from human neocortical and hippocampal tissues. However, little data are available on hNS/PCs obtained from the adult human amygdala.Materials and Methods: The present study explored the capacity of the amygdala harvested from resected brain tissues of patients with medically refractory epilepsy to generate neurosphere-like bodies and motor neuron-like cells.Results: Although the proliferation process was slow, a considerable amount of cells was obtained after the 3rd passage. In addition, the cells could generate motor neuron-like cells under appropriate culture conditions. Conclusion: Isolation and culture of these cells enable us to improve our knowledge of the role of the amygdala in some neurological and psychological disorders and provide a novel source for therapeutic cell transplantation.}, keywords = {Brain,Hippocampus,Intractable epilepsy,Motor Neuron,Neural stem cells}, url = {https://ijbms.mums.ac.ir/article_11548.html}, eprint = {https://ijbms.mums.ac.ir/article_11548_a7bde3b2eda8806114262f5bd4e373cf.pdf} }