@article { author = {Nadimi, Hoda and Djazayery, Abolghassem and Javanbakht, Mohammad Hassan and Dehpour, Ahmadreza and Ghaedi, Ehsan and Derakhshanian, Hoda and Mohammadi, Hamed and Mousavi, Seyedeh Neda and Djalali, Mahmoud}, title = {Effect of vitamin D supplementation on CREB-TrkB-BDNF pathway in the hippocampus of diabetic rats}, journal = {Iranian Journal of Basic Medical Sciences}, volume = {23}, number = {1}, pages = {117-123}, year = {2020}, publisher = {Mashhad University of Medical Sciences}, issn = {2008-3866}, eissn = {2008-3874}, doi = {10.22038/ijbms.2019.38170.9068}, abstract = {Objective(s): Cyclic AMP (adenosine monophosphate) response element-binding protein (CREB) and Brain-derived neurotrophic factor (BDNF) are reported to broadly involve in learning capacity and memory. BDNF exerts its functions via tropomyosin receptor kinase B (TrkB). BDNF transcription is regulated by stimulating CREB phosphorylation. The CREB-TrkB-BDNF pathway is reported to be affected by diabetes, which may contribute to its cognitive deficits. This study was conducted to investigate the effect of vitamin D supplementation on the hippocampal fraction of this pathway in an animal model of type-1 diabetes mellitus (T1DM). Materials and Methods: Thirty-six adult male Sprague-Dawley rats were randomly divided into 4 groups as follows: Group 1: normal healthy rats (n=8); group 2: normal healthy rats receiving sesame oil supplementation as placebo (n=8); Group 3: diabetic rats receiving sesame oil (n=10); and Group 4: diabetic rats treated with 4300 IU/kg/week vitamin D dissolved in sesame oil (n=10). Diabetes was induced by intraperitoneal (IP) injection of streptozotocin. Blood and hippocampal samples were acquired at the end of the experiment. RNA was extracted from the hippocampus, and real-time PCR (polymerase chain reaction) was performed for BDNF and TrkB gene expression. Results: Administration of vitamin D (4300 IU/kg/week) in a T1DM animal model increased CREB phosphorylation in the hippocampus, but the serum and hippocampal BDNF levels and TrkB and BDNF gene expression did not change significantly.Conclusion: Vitamin D increased hippocampal CREB phosphorylation in a T1DM animal model. Our findings showed that vitamin D might be protective against central nervous system complications in diabetes. However, future studies are warranted.}, keywords = {Brain-derived neurotrophic factor,CREB,Diabetes,TrkB,Vitamin D}, url = {https://ijbms.mums.ac.ir/article_14180.html}, eprint = {https://ijbms.mums.ac.ir/article_14180_b20dac115b1e2899cd1b1ec79c94fe42.pdf} }