@article { author = {Fang, Fang and Qin, Yingxin and Qi, Ling and Fang, Qing and Zhao, Liangzhong and Chen, Shuang and Li, Qiang and Zhang, Duo and Wang, Liguo}, title = {Juglone exerts antitumor effect in ovarian cancer cells}, journal = {Iranian Journal of Basic Medical Sciences}, volume = {18}, number = {6}, pages = {544-548}, year = {2015}, publisher = {Mashhad University of Medical Sciences}, issn = {2008-3866}, eissn = {2008-3874}, doi = {10.22038/ijbms.2015.4525}, abstract = {Objective(s): Juglone is isolated from many species of the Juglandaceae family and used as an anti-viral, anti-bacterial, and anti-tumor therapeutic. Here, we evaluated juglone-induced antitumor effect in ovarian cancer SKOV3 cells. Materials and Methods: MTT assay was performed to examine juglone anti-proliferative effect. Cell cycle and apoptosis were studied using flow cytometry in juglone-treated SKOV3 cells. To investigate molecular mechanism of cell cycle and apoptosis, protein expression levels were measured by Western blot analysis of cyclin D1, Bcl-2, Bax, cytochrome c, caspase-9 and caspase-3. To investigate the motility of juglone-treated SKOV3 cell, Matrigel invasion assay was employed to characterize cell invasion. Also, matrix metalloproteinase-2 (MMP-2) expression levels were detected by western blot. Results:Juglone significantly inhibited SKOV3 cell proliferation as shown by G0/G1 phase arrest, and this effect was mediated by inactivation of cyclin D1 protein (P<0.05). Juglone induced apoptosis in SKOV3 cell which was accompanied by caspase-9 and caspase-3 activation (P<0.05). Juglone decreased Bcl-2 levels and increased Bax and cytochrome c (Cyt c) levels(P<0.05). Juglone sufficiently inhibited invasion while evidently decreased MMP-2 expression (P<0.05). Conclusion:The results suggest that juglone could probably induce apoptosis through mitochondrial pathway and restrained cell invasiveness by decreasing MMP expression.}, keywords = {Apoptosis,Cell Cycle Arrest,Invasion,Juglone,Ovarian cancer}, url = {https://ijbms.mums.ac.ir/article_4525.html}, eprint = {https://ijbms.mums.ac.ir/article_4525_04a34c08957ce0223d3476f06a5c1240.pdf} }