@article { author = {Khodadadi, Ali and Pipelzadeh, Mohammad Hassan and Aghel, Nasrin and Esmaeilian, Majid and Zali, Iman}, title = {A Comparative Study upon the Therapeutic Indices of Some Natural and Synthetic Anti-inflammatory Agents}, journal = {Iranian Journal of Basic Medical Sciences}, volume = {14}, number = {4}, pages = {340-348}, year = {2011}, publisher = {Mashhad University of Medical Sciences}, issn = {2008-3866}, eissn = {2008-3874}, doi = {10.22038/ijbms.2011.5022}, abstract = {Objective(s) The aim of the present study was to compare the therapeutic indices of several agents used in treatment of inflammatory conditions which included: Vitamin E, hydro-alcoholic extracts of Glycyrrhiza glabra, Matricaria aurea, dexamethasone, piroxicam and diclofenac using Wehi-164 fibrosarcoma cells. Materials and Methods Cytotoxicity evaluation was based on vital dye exclusion assay. Matrix-metalloproteinases inhibition (MMPI) was assessed by gelatinase zymography method. The collected data were used to estimate the IC50 (50% MMPI concentration), LC50 (50% cytotoxicity concentration) and the therapeutic index (LC50/IC50). Results Among the natural anti-inflammatory agents used, M. aurea was the least toxic and the most effective inhibitor of MMP. Vitamin E not only increased MMP activity, but also was the most toxic of all the agents tested. Next in terms of toxicity to vitamin E was G. glabra. Diclofenac was more toxic than both piroxicam and dexamethasone. Conclusion Findings from this study suggest that medicinal plants reputed to have anti-inflammatory properties are not equally effective and safe. In order to assess the implications of these findings, further in vitro and in vivo studies are needed.}, keywords = {Antinflammatory agents,Cytotoxicity,Gelatinase,Matrix-metalloproteinases,Therapuetic Index,Zymography}, url = {https://ijbms.mums.ac.ir/article_5022.html}, eprint = {https://ijbms.mums.ac.ir/article_5022_9025b61efb2144637f8680ca03236cb5.pdf} }