@article { author = {Hajimoradi, Monire and Daneshmandi, Saeed and Hassan, Zuhair Mohammad and Roudbary, Maryam}, title = {Effect of Shark Liver Oil on Peritoneal Murine Macrophages in Responses to Killed-Candida albicans}, journal = {Iranian Journal of Basic Medical Sciences}, volume = {12}, number = {3}, pages = {179-183}, year = {2009}, publisher = {Mashhad University of Medical Sciences}, issn = {2008-3866}, eissn = {2008-3874}, doi = {10.22038/ijbms.2009.5180}, abstract = {Objective(s) Shark Liver Oil (SLO) is an immunomodulator. Macrophages play a key role in host defense against pathogens like fungi. Candida albicans have mechanisms to escape immune system. We determined the effect of killed-Candida on the in vitro viability of macrophages and the effect of SLO on augmentation of this potency. Materials and Methods Peritoneal macrophages were separated and cultured (3*105/well). At first, the effect of killed-Candida (200 cells/well) on macrophage viability was evaluated, using MTT test. Then, MTT was performed on macrophages stimulated with killed-Candida in the presence of SLO. Results Killed-Candida suppressed the ability of MTT reduction and hence macrophages viability (P=0.026), but addition of SLO (100 mg/ml) significantly enhanced cell viability (P=0.00). So, SLO could neutralize the inhibitory effect of Candida. Conclusion Simultaneous with cytotoxic effect of killed-Candida cells on macrophages viability, SLO augment macrophages viability. So, it can be applied in candidiasis as a complement.}, keywords = {Candida albicans,Macrophage,Shark,Liver Oil}, url = {https://ijbms.mums.ac.ir/article_5180.html}, eprint = {https://ijbms.mums.ac.ir/article_5180_3998da248577fec648a1209edfc7d29b.pdf} }