@article { author = {Shakibaie, Mohammad Reza and Shahcheraghi, Fereshteh and Hashemi, Ali and Adeli, N Saeed}, title = {Detection of TEM, SHV and PER Type Extended-Spectrum ß-Lactamase Genes among Clinical Strains of Pseudomonas aeruginosa Isolated from Burnt Patients at Shafa-Hospital, Kerman, Iran}, journal = {Iranian Journal of Basic Medical Sciences}, volume = {11}, number = {2}, pages = {104-111}, year = {2008}, publisher = {Mashhad University of Medical Sciences}, issn = {2008-3866}, eissn = {2008-3874}, doi = {10.22038/ijbms.2008.5220}, abstract = {Objective(s) This study was carried out to evaluate the existence of the TEM, SHV and PER ESBL genes in ESBL producing strains of Pseudomonas aeruginosa isolated from burnt patients at Shafa-hospital, Kerman, Iran. Materials and Methods A total of 120 strains of P. aeruginosa were isolated from 245 patients in burn unit of Shafa-hospital during January 2006 to December 2007. MIC of antibiotics was measured using agar dilution test. ESBL producing strains were detected by double-disc synergy method containing amoxicillin and amoxicillin+clavulanic acid and phenotypic confirmatory test. All the clinical isolates resistant to imipenem (IMP) were screened for  the production of MBL by E-test with IMP/IMP+EDTA strips. PCR and multiplex-PCR performed for the detection of different types of ESBL producing genes in ESBL positive isolates. Results Of 120 the isolates, 3-5% showed MIC greater than 16 µg/ml to IMP and meropenem, 66% showed MIC greater than 32 µg/ml to ceftazidime, 42% to azteronam and 60% of the isolates showed MIC greater than  64 µg/ml to cefotaxime, 41 (34%) confirmed as ESBL producers. Not any isolate could produce MBL (P≤0.05). The PCR assay of all ESBL producing isolates revealed that 6.6%, 4.1% and 2.5% of them were positive for SHV, PER and TEM genes, respectively. Conclusion Many ESBL producing strains of P. aeruginosa isolated from patients in burn unit of Shafa-hospital. However, none could produce MBL enzyme. The genes among ESBL producing strains were SHV, PER as well as TEM type of β-lacatamases. }, keywords = {Antibiotic resistance,ESBL,MBL,Pseudomonas aeruginosa}, url = {https://ijbms.mums.ac.ir/article_5220.html}, eprint = {https://ijbms.mums.ac.ir/article_5220_c2acfcf1fb7ae220117f6b16cbd651e6.pdf} }