@article { author = {Zhang, Hongxia and Zhang, Xiaoning and Yuan, Xiaoying and Wang, Linna and Xiao, Ying}, title = {MicroRNA-205 inhibits renal cells apoptosis via targeting CMTM4}, journal = {Iranian Journal of Basic Medical Sciences}, volume = {18}, number = {10}, pages = {1020-1026}, year = {2015}, publisher = {Mashhad University of Medical Sciences}, issn = {2008-3866}, eissn = {2008-3874}, doi = {10.22038/ijbms.2015.5467}, abstract = {Objective(s):MicroRNAs (miRNAs) are small non-coding RNA molecules that regulate gene expression. They have important roles in kidney development, homeostasis and disease, and participate in the onset and progression of tubulointerstitial sclerosis and end-stage glomerular lesions that occur in various forms of chronic kidney disease (CKD). In the present study, we elucidated the role of microRNA 205 (miR-205) in cisplatin-induced renal cell apoptosis and explored the molecular mechanisms. Materials and Methods:The chronic interstitial nephropathy rat model was induced, and the miRNA expression profile in the kidney cells from rats with CKD was screened. Cisplatin-induced apoptosis in normal renal HK-2 cells was evaluated using flow cytometry, and regulation of miR-205 on target gene was validated using luciferase assay, western blot and real time PCR assays. Results: We found that miR-205 expression was significantly decreased in the cells from kidney of CKD rat (P<0.01). Our data showed that when miR-205 was overexpressed or silenced using the mimic or inhibitor, the percentages of apoptotic cells were suppressed or increased significantly (P<0.05), respectively. Moreover, we have identified CMTM4 gene, which is involved in cell proliferation and apoptosis, as a novel target for miR-205. In addition, miR-205 could inhibit apoptosis by binding to the 3’UTR of CMTM4 mRNA and inhibiting its transcriptional activity. Conclusion: This study elucidated that miR-205 plays an important role in the regulation of apoptosis in renal cells, suggesting a potential therapeutic target to hinder CKD development.}, keywords = {Apoptosis,chronic kidney disease,CMTM4,microRNA 205}, url = {https://ijbms.mums.ac.ir/article_5467.html}, eprint = {https://ijbms.mums.ac.ir/article_5467_a3c8874c52da77c245df6d0de3060fc6.pdf} }