@article { author = {Tabaei, Samira and Mashkani, Baratali and Esmaili, Arezoo and Karimi, Reza and Jamehdar, Saeid Amel}, title = {Design of cocktail peptide vaccine against Cytomegalovirus infection}, journal = {Iranian Journal of Basic Medical Sciences}, volume = {19}, number = {4}, pages = {449-454}, year = {2016}, publisher = {Mashhad University of Medical Sciences}, issn = {2008-3866}, eissn = {2008-3874}, doi = {10.22038/ijbms.2016.6819}, abstract = {Objective(s):Human Cytomegalovirus (HCMV) remains a major morbidity and mortality cause in immuno suppressed patients. Therefore, significant effort has been made towards the development of a vaccine. In this study, the expression of the pp65 and gB fusion peptides and Fc domain of mouse IgG2a as a novel delivery system for selective uptake of antigens by antigen-presenting cells (APCs) in Pichia pastoris yeast system were studied. Materials and Method: In this study, four immune dominant sequences in pp65 protein and 3 immuno dominant sequences in gB protein were selected according to literature review. Peptide linker -GGGGS- was used for construction of fusion peptide. This fusion peptide was cloned in the pPICZαA expression vector and transfected into P. pastoris host cells. Results: Dot blot and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) techniques showed that a high level of pp65-gB-Fc fusion peptide was expressed. Conclusion: This CMV pp65-gB-Fc fusion peptide could be a promising candidate for the development of a novel peptide vaccine.}, keywords = {Human cytomegalovirus,pp65,Pichia pastoris,Peptide vaccine}, url = {https://ijbms.mums.ac.ir/article_6819.html}, eprint = {https://ijbms.mums.ac.ir/article_6819_0b5ddae262f587de6498b818b339ef9d.pdf} }