@article { author = {Min Park, Seung and Lee, Jae-Chul and Hui Chen, Bai and Shin, Bich-Na and Hwi Cho, Jeong and Hye Kim, In and Ha Park, Joon and Won, Moo-Ho and Hyeon Ahn, Ji and Tae, Hyun-Jin and Cheol Shin, Myoung and Woo Park, Chan and Hwi Cho, Jun and Young Lee, Hui}, title = {Difference in transient ischemia-induced neuronal damage and glucose transporter-1 immunoreactivity in the hippocampus between adult and young gerbils}, journal = {Iranian Journal of Basic Medical Sciences}, volume = {19}, number = {5}, pages = {521-528}, year = {2016}, publisher = {Mashhad University of Medical Sciences}, issn = {2008-3866}, eissn = {2008-3874}, doi = {10.22038/ijbms.2016.6937}, abstract = {Objective(s): The alteration of glucose transporters is closely related with the pathogenesis of brain edema. We compared neuronal damage/death in the hippocampus between adult and young gerbils following transient cerebral ischemia/reperfusion and changes of glucose transporter-1(GLUT-1)-immunoreactive microvessels in their ischemic hippocampal CA1 region. Materials and Methods: Transient cerebral ischemia was developed by 5-min occlusion of both common carotid arteries. Neuronal damage was examined by cresyl violet staining, NeuN immunohistochemistry and Fluoro-Jade B histofluorescence staining and changes in GLUT-1 expression was carried out by immunohistochemistry. Results: About 90% of pyramidal neurons only in the adult CA1 region were damaged after ischemia/reperfusion; in the young, about 53 % of pyramidal neurons were damaged from 7 days after ischemia/reperfusion. The density of GLUT-1-immunoreactive microvessels was significantly higher in the young sham-group than that in the adult sham-group. In the ischemia-operated-groups, the density of GLUT-1-immunoreactive microvessels was significantly decreased in the adult and young at 1 and 4 days post-ischemia, respectively, thereafter, the density of GLUT-1-immunoreactive microvessels was gradually increased in both groups after ischemia/reperfusion. Conclusion: CA1 pyramidal neurons of the young gerbil were damaged much later than that in the adult and that GLUT-1-immunoreactive microvessels were significantly decreased later in the young. These data indicate that GLUT-1 might differently contribute to neuronal damage according to age after ischemic insults.}, keywords = {CA1 region,Delayed neuronal death,Ischemia/reperfusion injury Pyramidal neurons,Young gerbil}, url = {https://ijbms.mums.ac.ir/article_6937.html}, eprint = {https://ijbms.mums.ac.ir/article_6937_5a27eb6ae681a63a82faf2ec4d265eaf.pdf} }