@article { author = {Fattahi Abdizadeh, Mojtaba and Makvandi, Manoochehr and Samarbafzadeh, Alireza and Azadmanesh, Kayhan}, title = {A novel medium-throughput biological assay system for HTLV-1 infectivity and drug discovery}, journal = {Iranian Journal of Basic Medical Sciences}, volume = {20}, number = {10}, pages = {1109-1118}, year = {2017}, publisher = {Mashhad University of Medical Sciences}, issn = {2008-3866}, eissn = {2008-3874}, doi = {10.22038/ijbms.2017.9417}, abstract = {Objective(s): Here, a reporter cell line containing two reporter vectors were developed, in order to monitor the Human T-Lymphotropic Virus type1(HTLV-1) infectivity and the cell viability simultaneously. Materials and Methods: The reporter cell line was constructed by stably transfected baby hamster's kidney cell line (BHK-21), with the genomes expressing two different reporters in separate plasmids.The first reporter gene is transactivated by the HTLV-1 tax protein, while the second reporter is continuously expressed when introduced into a mammalian cell. In order to show its functionality, the effect of the drug mix on HTLV-1 was assayed by this system and was compared to the results obtained by other methods. Results: HTLV-1 reporter cell line was found to produce high level of luciferase when co-cultured with MT-2 and Hut-102 cells but not with Jurkat cell. Moreover, the combination therapy against HTLV-1 can reduce luciferase expression of the cell when co-cultured with MT-2 and Hut-102 comparable to the ELISA (R=0.932, P-value =0.002). In addition, the results revealed the superiority of the present system over the molecular methods. Conclusion: The results demonstrated that the biological assay system is a beneficial tool for the medium-throughput anti-HTLV-1 drug screening and inhibitory effect.}, keywords = {Biological Assay,Drug screening,HTLV-1,Hut102,Luciferase,Reporter gene}, url = {https://ijbms.mums.ac.ir/article_9417.html}, eprint = {https://ijbms.mums.ac.ir/article_9417_04ec4c75273a85322aff84c4fc11522d.pdf} }