%0 Journal Article %T The efficacy and molecular mechanism of the effect of schisandrin b on the treatment of erectile dysfunction %J Iranian Journal of Basic Medical Sciences %I Mashhad University of Medical Sciences %Z 2008-3866 %A LIU, WEI %A ZHAO, CHEN %A Huang, Yanping %A Liu, Yidong %A Lu, Mujun %D 2019 %\ 08/01/2019 %V 22 %N 8 %P 866-871 %! The efficacy and molecular mechanism of the effect of schisandrin b on the treatment of erectile dysfunction %K cAMP-PKA pathway %K Cyclic nucleotides %K Erectile dysfunction %K Intracavernosum pressure %K NO-cGMP pathway %K Schisandrin b %R 10.22038/ijbms.2019.27455.6701 %X Objective(s): The purpose of this study is to determine the efficacy and molecular mechanism of the effect of schisandrin b (SCHB) on treating erectile dysfunction (ED) in a rat model with bilateral cavernous crushing nerve injury. Materials and Methods: The ED rat model was established with bilateral cavernous nerve crushing, and then confirmed by apomorphine. Fifty healthy eight-week-old ED rats were randomly assigned into five group, including control group (sham surgery), bilateral cavernous nerve crushing injury group (BCNC), BCNC with low SCHB (100 mg/d), BCNC with medium SCHB (200 mg/d) and BCNC with high SCHB (400 mg/d). For the last three groups, SCHB was given for 2 months. Then, we examined intracavernosal pressure (ICP), cyclic nucleotides (cAMP, cGMP), endothelial nitric oxide synthase (eNOS) and neuronal NOS (nNOS) in all groups. Results: In the study of ICP, SCHB was able to improve ED in a dose-dependent manner. In addition, as compared to the BCNC group, the relative expression of eNOS and nNOS in medium and high concentration of SCHB-treated groups are higher than BCNC group. Moreover, all groups treated with SCHB showed a significant higher expression level of cAMP and cGMP.Conclusion: These results suggested that SCHB were able to significantly improve the ED on rat model through the NO-cGMP and cAMP- protein kinase A (PKA) pathway. %U https://ijbms.mums.ac.ir/article_13320_81d0227600cb7fed94652d91e63a3d8c.pdf