%0 Journal Article %T Early oleate deficiency leads to severe defects in fetal rat liver development %J Iranian Journal of Basic Medical Sciences %I Mashhad University of Medical Sciences %Z 2008-3866 %A Mohammadzadeh, Fatemeh %A Alihemmati, Alireza %A Pirpour Tazehkand, Abbas %A Darabi, Masoud %A Mehdizadeh, Amir %D 2019 %\ 09/01/2019 %V 22 %N 9 %P 1010-1015 %! Early oleate deficiency leads to severe defects in fetal rat liver development %K Development %K Embryo %K Hepatocytes %K Monounsaturated fatty acids %K pregnancy %R 10.22038/ijbms.2019.35084.8345 %X Objective(s): Oleate can be produced through de novo synthesis, which contributes to biological processes and signaling pathways. However, the role of this non-essential fatty acid in hepatic development remains unclear. The current study aimed to evaluate the influence of early oleate deficiency induced by the inhibitor of de novo oleate synthesis MF-438 on fetal rat liver development.Materials and Methods: Female Wistar rats with an average weight of 200±20 g were subjected to this study. After mating, pregnant rats were divided into three groups and gavaged with the vehicle, MF 438 or MF-438 plus oleate from day 3 of pregnancy for five days. Obtained fetuses were sacrificed and the liver tissues were retrieved. Hepatic morphological index, biochemical markers, and gene expression of hepatic development markers were analyzed using Hematoxylin-Eosine, spectrometry, and real-time PCR techniques, respectively.Results: Relatively, deficient morphological indices and hepatic maturation markers were observed in fetus livers of the inhibitor-treated group. In comparison to the other two groups, total hepatic protein and glycogen content were increased with treatment of MF-438 plus oleate. Hepatocyte nuclear factor 1α, alpha fetoprotein, albumin, and cytochrome P450 gene expression were also significantly increased in the group treated with both MF-438 and oleate.Conclusion: Our data indicate that oleate availability during early embryo development is linked with fetal rat liver development. %U https://ijbms.mums.ac.ir/article_13533_1e9167cb23a9707608805d6d771e157b.pdf