%0 Journal Article %T Effect of vitamin D supplementation on CREB-TrkB-BDNF pathway in the hippocampus of diabetic rats %J Iranian Journal of Basic Medical Sciences %I Mashhad University of Medical Sciences %Z 2008-3866 %A Nadimi, Hoda %A Djazayery, Abolghassem %A Javanbakht, Mohammad Hassan %A Dehpour, Ahmadreza %A Ghaedi, Ehsan %A Derakhshanian, Hoda %A Mohammadi, Hamed %A Mousavi, Seyedeh Neda %A Djalali, Mahmoud %D 2020 %\ 01/01/2020 %V 23 %N 1 %P 117-123 %! Effect of vitamin D supplementation on CREB-TrkB-BDNF pathway in the hippocampus of diabetic rats %K Brain-derived neurotrophic factor %K CREB %K Diabetes %K TrkB %K Vitamin D %R 10.22038/ijbms.2019.38170.9068 %X Objective(s): Cyclic AMP (adenosine monophosphate) response element-binding protein (CREB) and Brain-derived neurotrophic factor (BDNF) are reported to broadly involve in learning capacity and memory. BDNF exerts its functions via tropomyosin receptor kinase B (TrkB). BDNF transcription is regulated by stimulating CREB phosphorylation. The CREB-TrkB-BDNF pathway is reported to be affected by diabetes, which may contribute to its cognitive deficits. This study was conducted to investigate the effect of vitamin D supplementation on the hippocampal fraction of this pathway in an animal model of type-1 diabetes mellitus (T1DM). Materials and Methods: Thirty-six adult male Sprague-Dawley rats were randomly divided into 4 groups as follows: Group 1: normal healthy rats (n=8); group 2: normal healthy rats receiving sesame oil supplementation as placebo (n=8); Group 3: diabetic rats receiving sesame oil (n=10); and Group 4: diabetic rats treated with 4300 IU/kg/week vitamin D dissolved in sesame oil (n=10). Diabetes was induced by intraperitoneal (IP) injection of streptozotocin. Blood and hippocampal samples were acquired at the end of the experiment. RNA was extracted from the hippocampus, and real-time PCR (polymerase chain reaction) was performed for BDNF and TrkB gene expression. Results: Administration of vitamin D (4300 IU/kg/week) in a T1DM animal model increased CREB phosphorylation in the hippocampus, but the serum and hippocampal BDNF levels and TrkB and BDNF gene expression did not change significantly.Conclusion: Vitamin D increased hippocampal CREB phosphorylation in a T1DM animal model. Our findings showed that vitamin D might be protective against central nervous system complications in diabetes. However, future studies are warranted. %U https://ijbms.mums.ac.ir/article_14180_b20dac115b1e2899cd1b1ec79c94fe42.pdf