%0 Journal Article %T Evaluation of antibacterial activity of enterocin A-colicin E1 fusion peptide %J Iranian Journal of Basic Medical Sciences %I Mashhad University of Medical Sciences %Z 2008-3866 %A Fathizadeh, Hadis %A Saffari, Mahmood %A Esmaeili, Davoud %A Moniri, Rezvan %A Salimian, Morteza %D 2020 %\ 11/01/2020 %V 23 %N 11 %P 1471-1479 %! Evaluation of antibacterial activity of enterocin A-colicin E1 fusion peptide %K Antibacterial activity %K Bacteriocins %K Colicin E1 %K Enterocin A %K Fusion peptide %R 10.22038/ijbms.2020.47826.11004 %X Objective(s): Bacterial resistance to most common antibiotics is a harbinger of the requirement to find novel anti-infective, antimicrobials agents, and increase innovative strategies to struggle them. Numerous bacteria produce small peptides with antimicrobial activities called bacteriocin. This study aimed to investigate the antibacterial properties of the fusion protein of Enterocin A and Colicin E1 modified against pathogens.Materials and Methods: Analysis of recombinant bacteriocin Enterocin A and Colicin E1 (ent A-col E1) was performed to assay the stability and antibacterial activity of this fusion protein. The pET-22b vector was employed to express the coding sequence of the ent A-col E1 peptide in Escherichia coli BL21 (DE3). Minimum inhibitory concentration (MIC), disk diffusion, and time-kill tests were performed to evaluate the antibacterial activity of the ent A-col E1 against Pseudomonas aeruginosa (ATCC 9027), Escherichia coli (ATCC 10536), Enterococcus faecalis (ATCC 29212), and Staphylococcus aureus (ATCC 33591).Results: The suggested recombinant peptide had good antibacterial activity against both Gram-negative and Gram-positive pathogens. It has also good stability at various temperatures, pH levels, and salt concentrations.Conclusion: Because bacteriocins are harmless compounds, they can be recommended as therapeutic or preventive supplements to control pathogens. According to the obtained results, the ent A-col E1 peptide can serve as an efficient antibacterial compound to treat or prevent bacterial infections. %U https://ijbms.mums.ac.ir/article_16640_958d331f9ce8afa2922454971acecc8c.pdf