%0 Journal Article %T DNA vaccine containing Flagellin A gene induces significant immune responses against Helicobacter pylori infection: An in vivo study %J Iranian Journal of Basic Medical Sciences %I Mashhad University of Medical Sciences %Z 2008-3866 %A Ansari, Hossein %A Tahmasebi Birgani, Maryam %A Bijanzadeh, Mahdi %D 2021 %\ 06/01/2021 %V 24 %N 6 %P 796-804 %! DNA vaccine containing Flagellin A gene induces significant immune responses against Helicobacter pylori infection: An in vivo study %K DNA vaccine %K flaA protein %K Flagellin %K Helicobacter pylori %K Immunomodulation %K In vivo %R 10.22038/ijbms.2021.54415.12227 %X Objective(s): Helicobacter pylori is one of the most prevalent human infectious agents that is directly involved in various upper digestive tract diseases. Although antibiotics-based therapy and proton pump inhibitors eradicate the bacteria mostly, their effectiveness has been declined recently due to emergence of antibiotic-resistant strains. Development of a DNA vaccine is a promising approach against bacterial pathogens. Genes encoding motility factors are promising immunogens to develop a DNA vaccine against H. pylori infection due to critical role of these genes in bacterial attachment and colonization within the gastric lumen. The present study aimed to synthesize a DNA vaccine construct based on the Flagellin A gene (flaA), the predominant flagellin subunit in H. pylori flagella. Materials and Methods: The coding sequence of flaA was amplified through PCR and sub-cloned in the pBudCE4.1 vector. The recombinant vector was introduced into the human dermal fibroblast cells, and its potency to express the flaA protein was analyzed using SDS-PAGE. The recombinant construct was intramuscularly (IM) injected into the mice, and the profiles of cytokines and immunoglobulins were measured using ELISA. Results: It has been found that flaA was successfully expressed in cells. Recombinant-vector also increased the serum levels of evaluated cytokines and immunoglobulins in mice. Conclusion: These findings showed that the pBudCE4.1-flaA construct was able to activate the immune responses. This study is the first step towards synthesis of recombinant-construct based on the flaA gene. Immunization with such construct may inhibit the H. pylori-associated infection; however, further experiments are urgent. %U https://ijbms.mums.ac.ir/article_18126_262bc8f5222fdc0cd253dd4f3f2f130e.pdf