%0 Journal Article %T MiR-574-5p promotes cell proliferation by negatively regulating small C-terminal domain phosphatase 1 in esophageal squamous cell carcinoma %J Iranian Journal of Basic Medical Sciences %I Mashhad University of Medical Sciences %Z 2008-3866 %A Zhao, Chunming %A Liu, Jialin %A Xu, Yong %A Guo, Jiamei %A Wang, Liping %A Chen, Linfen %A Xu, Lina %A Dong, Guokai %A Zheng, Wei %A Li, Zhouru %A Cai, Hongxing %A Li, Shanshan %D 2022 %\ 10/01/2022 %V 25 %N 10 %P 1243-1250 %! MiR-574-5p promotes cell proliferation by negatively regulating small C-terminal domain phosphatase 1 in esophageal squamous cell carcinoma %K Cell Proliferation %K CTDSP1 protein %K Esophageal squamous cell carcinoma %K MiR-574-5p %K Oncogene Protein v-akt %R 10.22038/ijbms.2022.65886.14492 %X Objective(s): Esophageal cancer is one of the most common cancers with high incidence and mortality rates, especially in China. MicroRNA (miRNA) can be used as a prognostic marker for various human cancers. This study aims to detect suitable miRNA markers for esophageal squamous cell carcinoma (ESCC). Materials and Methods: Our previous gene expression data of ESCC cells and the data from GSE43732 and GSE112840 were analyzed. The expression of miR-574-5p in ESCC patients and controls was analyzed by real-time quantitative PCR. The effect of miR-574-5p on proliferation was detected by real-time cell analysis (RTCA) and EdU proliferation assay after cell transfections. The target gene small C-terminal domain phosphatase 1 (CTDSP1) of miR-574-5p was validated by luciferase reporter assay and western blotting.Results: In the current study, the bioinformatics analysis found miR-574-5p up-regulated in ESCC. The qPCR assay of 26 ESCC and 13 adjacent/ normal tissues confirmed these results. We further demonstrated that miR-574-5p overexpression promoted cell proliferation. Then the dual-luciferase reporter assay and the rescue experiment suggested that CTDSP1 was a direct target of miR-574-5p.Conclusion: MiR-574-5p played an oncological role in ESCC by interacting and negatively regulating CTDSP1. These results provided a deeper understanding of the effect of miR-574-5p on ESCC. %U https://ijbms.mums.ac.ir/article_21028_36af7c18762942df8511dc3ad569dced.pdf