%0 Journal Article %T An in vitro investigation of the apoptosis-inducing activity of corosolic acid in breast cancer cells %J Iranian Journal of Basic Medical Sciences %I Mashhad University of Medical Sciences %Z 2008-3866 %A Abdalkareem Jasim, Saade %A Zedan Khalaf, Omer %A Hamoud Alshahrani, Shadia %A Hachem, Kadda %A Ziyadullaev, Shukhrat %A Turki Jalil, Abduladheem %A Wang, ChangMing %A S. Zabibah, Rahman %A A. Bin Jardan, Yousef %A A. Qasim, Qutaiba %A Suliman Maashi, Marwah %A Fakri Mustafa, Yasser %D 2023 %\ 04/01/2023 %V 26 %N 4 %P 453-460 %! An in vitro investigation of the apoptosis-inducing activity of corosolic acid in breast cancer cells %K Apoptosis %K Breast Cancer %K Corosolic acid %K JAK2 %K STAT3 %R 10.22038/ijbms.2023.67783.14834 %X Objective(s): Breast cancer is the most prevalent cancer among females with different molecular subtypes. Corosolic acid is a pentacyclic triterpenoid with anti-cancer properties. Materials and Methods: The MTT assay was used to assess the cytotoxic activity of corosolic acid on MDA-MB-231 and MCF7 cell lines. To determine the apoptotic cells, the flow cytometry technique was utilized. The expression levels of apoptosis-related genes and proteins were quantified using quantitative real time-PCR (qRT-PCR) and Western blotting methods. The activity of caspase enzymes was measured by spectrophotometry.  Results: Corosolic acid significantly inhibited the proliferation of both cell lines compared with controls. This agent markedly induced apoptosis in MDA-MB-231 cells but did not affect MCF7 cells compared with controls. Treating the MADA-MB-231 and MCF7 cell lines with corosolic acid showed an inducing effect on apoptosis-associated caspases, including Caspase-8, 9, and -3, in MADA-MB-231 cells with no effect on apoptotic markers in MCF7 cells. Further experiments uncovered corosolic acid-induced apoptosis in MADA-MB-231 cells by decreasing the expression of the phosphorylated form of JAK2 and STAT3 proteins.Conclusion: The present data suggested that corosolic acid is an apoptosis-inducing phytochemical in triple-negative breast cancer MADA-MB-231 cells. Also, corosolic acid triggered apoptosis in these cells by stimulating both pathways of apoptosis and inhibiting the JAK/STAT signaling. Furthermore, corosolic acid was found to inhibit MCF7 cell proliferation by a non-apoptotic mechanism. %U https://ijbms.mums.ac.ir/article_21754_1bf8c021eea9c737eb0ac958107d5168.pdf