%0 Journal Article %T Effect of leptin receptor Q223R polymorphism on breast cancer risk %J Iranian Journal of Basic Medical Sciences %I Mashhad University of Medical Sciences %Z 2008-3866 %A Mohammadzadeh, Ghorban %A Ghaffari, Mohammad-Ali %A Bafandeh, Ahmmad %A Hosseini, Sayed-Mohammad %D 2014 %\ 08/01/2014 %V 17 %N 8 %P 588-594 %! Effect of leptin receptor Q223R polymorphism on breast cancer risk %K Breast Cancer %K Leptin receptor rene polymorphism %K Polymerase chain reaction %K Restriction fragment length polymorphism %R 10.22038/ijbms.2014.3192 %X Objective(s):Leptin receptor (LEPR) is a member of the class I cytokine receptor super-family that is known implicated in the initiation and progression of breast cancer. We have investigated the effect of Q223R polymorphism on the breast cancer susceptibly in a sample of Iranian subjects. Materials and Methods:We utilized a polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP) method to investigate the association ofLEPRQ223R polymorphism with breast cancer risk in a case control study consisting of 100 breast cancer cases and 100 controls without breast cancer. Serum levels of leptin and soluble leptin receptor (sOB-R) were measured by ELISA method. Results:The genotype (QQ, QR, and RR) distributions were 25, 56, and 19 % in breast cancer cases and 54, 40, and 6% in controls, respectively. The frequency of 223 RR genotype was significantly elevated in breast cancer cases as compared to controls (χ2= 20.072, P<0.001). Similar significance differences were also found in allele frequencies for Q and R between two groups (χ2= 19.027, P< 0.001). Additionally, there weresignificant association between Q223R genotypes and breast cancer risk; homozygotes for RR genotype (OR= 6.840; 95% confidence interval [CI] = 2.434-19.218), heterozygotes for QR (OR=3.024; 95% CI = 1.620-5.644, P = 0.001), and QR+RR genotype (OR= 3.522; 95% CI = 1.934-6.414, P < 0.001), respectively. Conclusion:Our results showed thattheLEPRQ223R polymorphism is associated with increased breast cancer risk as well as tumor grade in a sample of Iranian subjects. %U https://ijbms.mums.ac.ir/article_3192_8b55f8e83e9631f6c8ad1264be321fc1.pdf