%0 Journal Article %T Rapamycin protects testes against germ cell apoptosis and oxidative stress induced by testicular ischemia-reperfusion %J Iranian Journal of Basic Medical Sciences %I Mashhad University of Medical Sciences %Z 2008-3866 %A Ghasemnejad-berenji, Morteza %A Ghazi-Khansari, Mahmoud %A Yazdani, Iraj %A Saeedi Saravi, Seyed Soheil %A Nobakht, Maliheh %A Abdollahi, Alireza %A Mohajer Ansari, Javad %A Ghasemnejad-berenji, Hojjat %A Pashapour, Sarvin %A Dehpour, Ahmad Reza %D 2017 %\ 08/01/2017 %V 20 %N 8 %P 905-911 %! Rapamycin protects testes against germ cell apoptosis and oxidative stress induced by testicular ischemia-reperfusion %K Apoptosis %K Ischemia-reperfusion %K Rapamycin %K Testicular torsion %K Testis %R 10.22038/ijbms.2017.9112 %X Objective(s):Rapamycin is an immunosuppressant compound with a broad spectrum of pharmaco-logical activities. In recent years, it has been used successfully to decrease ischemia-reperfusion injury in several organ systems. The purpose of the present study was to examine the effect of rapamycin on testicular ischemia-reperfusion injury. Materials and Methods: Seventy-two adult male Wistar rats were divided into six groups: control (group1), sham-operated (Group2), T/D + DMSO as vehicle group (group3), and groups 4–6; respectively received 0.5, 1, and 1.5 mgkg-1 of rapamycin , IP 30 min before detorsion. Ischemia was achieved by twisting the right testis 720o clockwise for 1 hr. The right testis of 6 animals from each group were excised 4 hr after detorsion for the measurement of lipid peroxidation, caspase-3, and antioxidant enzyme activities. Histopathological changes and germ cell apoptosis were determined by measuring mean of seminiferous tubules diameters (MSTD) and TUNEL test in right testis of 6 animals per group, 24 hr after detorsion. Results: Testicular T/D caused increases in the apoptosis, malondialdehyde (MDA), and caspase-3 levels and decreases in the superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities in ipsilateral testis (P<0.001). The rats treated with rapamycin had significant decreases in the MDA and caspase-3 levels and significant increases in the SOD, CAT and GPx activities in ipsilateral testis compared with the T/D group (P<0.001); germ cell apoptosis was decreased, and MSTD was improved. Conclusion: Rapamycin administration during testicular torsion decreased ischemia/reperfusion (I/R) cellular damage. %U https://ijbms.mums.ac.ir/article_9112_2480429728b15b9e5f7f0e1d6444d259.pdf