ORIGINAL_ARTICLE
Recent advances in development of nano-carriers for immunogene therapy in various complex disorders
Immunotherapy is a novel preference for the treatment of various complex diseases. Considering the application of varying agents for suppression or activation of the immune system, immunogene therapy was confirmed to stand as a proper alternative for other immunotherapeutic strategies due to its capability in targeting cells with more specificity that leads to controlling the expression of therapeutic genes. This method facilitates the local and single-dose application of most gene therapies that result in the usage of high therapeutic doses with a low risk of systemic side effects while being cost-efficient in long-term administrations. However, the existing barriers between the administration site and cell nucleus limited the clinical uses of genetic materials. These challenges can be overcome through the promising method of exerting non-carriers with high stability, low toxicity/immunogenicity, and simple modifications. In this study, we attempted to review the potential of nanoparticle application throughout the immunogene therapy of different diseases including cancer, microbial diseases, allergies, inflammatory bowel disease, rheumatoid arthritis, and respiratory infections. We included the outline of some challenges and opportunities in regards to the delivery of genetic materials that are based on nano-systems through immunotherapy of these disorders. Next to the promising future of these vectors, more detailed analyses are required to overcome the current limitations in clinical approaches.
https://ijbms.mums.ac.ir/article_19631_99da24f84704970cf71d4b13582bc0f0.pdf
2022-02-01
134
147
10.22038/ijbms.2022.59718.13245
Gene delivery
Immunogene therapy
Immunotherapy
Nano-carriers
Sanaz
Shahgordi
sanaz.sh.70@gmail.com
1
Immunology Department, Faculty of Medicine, Golestan University of Medical Science, Gorgan, Iran
AUTHOR
Fatemeh
Oroojalian
oroojalian.f@gmail.com
2
Department of Advanced Sciences and Technologies, North Khorasan University of Medical Sciences, Bojnurd, Iran
AUTHOR
Ezzat
Hashemi
hashemi_nad@yahoo.com
3
Department of Neurology and Neurological Sciences, Stanford University, Stanford, CA, USA
AUTHOR
Maryam
Hashemi
hashemim@mums.ac.ir
4
Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
LEAD_AUTHOR
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141
ORIGINAL_ARTICLE
Calcium dobesilate ameliorates hepatorenal injury induced by carbon tetrachloride in mice
Objective(s): Calcium dobesilate (CaD) has anti-oxidant, anti-inflammatory, and anti-apoptotic effects. In this study, the protective effects of CaD against hepatorenal damage induced by carbon tetrachloride (CCl4) in mice were evaluated. Materials and Methods: Thirty male mice were randomly divided into five groups: Control, CaD 100 mg/kg, CCl4, CCl4+CaD 50 mg/kg, and CCl4+CaD 100 mg/kg. CaD (50 and 100 mg/kg) was administered orally once a day for 4 weeks. The liver and kidney indices (serum creatinine, blood urine nitrogen, alanine aminotransferase, and aspartate aminotransferase levels) were determined. Also, liver and kidney tissue oxidant/anti-oxidant markers (glutathione peroxidase, malondialdehyde, total anti-oxidant capacity, and superoxide dismutase) were measured. Cleaved caspase-3, Bax, cytochrome-c, and Bcl-2 protein levels were measured by immunoblotting method in the liver and kidney tissues. The liver and kidney histopathological changes were evaluated by the Hematoxylin and Eosin (H&E) staining method.Results: CCl4 induced significant oxidative stress and apoptosis in kidney and liver tissues that was concomitant with histopathological abnormalities in these organs in the CCl4 group versus the control (P<0.05). However, CaD (100 mg/kg) could significantly improve the histopathological change in the liver and kidney tissues of CCl4+CaD 100 mg/kg mice versus the CCl4 group (P<0.05). In addition, CaD (100 mg/kg) attenuated the pro and anti-apoptotic markers in the liver and kidney tissues of CCl4+CaD 100 mg/kg mice versus the CCl4 group (P<0.05).Conclusion: CaD (100 mg/kg) has a protective effect against hepatorenal injury induced by CCl4 at least via its anti-apoptotic and anti-oxidant properties.
https://ijbms.mums.ac.ir/article_19629_17a1129921d4f10ce751cc47ac657545.pdf
2022-02-01
148
154
10.22038/ijbms.2022.61499.13606
Apoptosis
Calcium dobesilate
Carbon tetrachloride
Mice
Oxidative stress
Elham
Hakimizadeh
hakimizadeh_elham@yahoo.com
1
Physiology-Pharmacology Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan,Iran
AUTHOR
Ayat
Kaeidi
a.kayedi@gmail.com
2
Department of Physiology and Pharmacology, School of medicine. Rafsanjan University of Medical Sciences, Rafsanjan, Iran
AUTHOR
Mohammad reza
Rahmani
drrahmani.1347@gmail.com
3
Physiology-Pharmacology Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
AUTHOR
Mohammad
Allahtavakoli
allahtavakoli@gmail.com
4
Department of Physiology and Pharmacology, School of medicine. Rafsanjan University of Medical Sciences, Rafsanjan, Iran
AUTHOR
Jalal
Hassanshahi
hasanshahij@gmail.com
5
Department of Physiology and Pharmacology, School of medicine. Rafsanjan University of Medical Sciences, Rafsanjan, Iran
LEAD_AUTHOR
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41
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47
ORIGINAL_ARTICLE
Identification of molecular pathways and protein-protein interactions in adipose tissue-derived mesenchymal stromal cells (ASCs) under physiological oxygen concentration in a diabetic rat model
Objective(s): Adipose tissue-derived mesenchymal stromal cells (ASCs) are useful in cell-based therapy. However, it is well known that diabetes mellitus (DM) alters ASCs’ functionality. The majority of in vitro studies related to ASCs are developed under non-physiological oxygen conditions. Therefore, they may not reflect the full effects of DM on ASCs, in vivo. The main aim of the current study is to identify molecular pathways and underlying biological mechanisms affected by diabetes on ASCs in physiological oxygen conditions.Materials and Methods: ASCs derived from healthy (ASCs-C) and diabetic (ASCs-D) rats were expanded under standard culture conditions (21% O2) or cultured in physiological oxygen conditions (3% O2) and characterized. Differential gene expressions (DEGs) of ASCs-D with respect to ASCs-C were identified and analyzed with bioinformatic tools. Protein-protein interaction (PPI) networks, from up- and down-regulated DEGs, were also constructed.Results: The bioinformatic analysis revealed 1354 up-regulated and 859 down-regulated DEGs in ASCs-D, with 21 and 78 terms over and under-represented, respectively. Terms linked with glycosylation and ribosomes were over-represented and terms related to the activity of RNA-polymerase II and transcription regulation were under-represented. PPI network disclosed RPL11-RPS5 and KDR-VEGFA as the main interactions from up- and down-regulated DEGs, respectively.Conclusion: These results provide valuable information about gene pathways and underlying molecular mechanisms by which diabetes disturbs ASCs biology in physiological oxygen conditions. Furthermore, they reveal, molecular targets to improve the use of ASCs in autologous transplantation.
https://ijbms.mums.ac.ir/article_19628_adfc4a7e3d7ddbe20e089f5cf6c9a498.pdf
2022-02-01
155
163
10.22038/ijbms.2022.59004.13107
Cell-based therapy
Diabetes
Enrichment analysis
Microarray
Physioxia
Luis Miguel
Paco Meza
siul_miguel1@hotmail.com
1
Maimonides Institute of Biomedical Research in Cordoba (IMIBIC), Spain. Avenida Menéndez Pidal s/n, CP 14004 Córdoba, Spain
AUTHOR
MDolores
Carmona
mariadolores.carmona@imibic.org
2
Maimonides Institute of Biomedical Research in Cordoba (IMIBIC), Spain. Avenida Menéndez Pidal s/n, CP 14004 Córdoba, Spain
AUTHOR
Sagrario
Canadillas
sagrita99@gmail.com
3
Maimonides Institute of Biomedical Research in Cordoba (IMIBIC), Spain. Avenida Menéndez Pidal s/n, CP 14004 Córdoba, Spain
AUTHOR
Ana
Lopez-Diaz
analopezd2212@gmail.com
4
Maimonides Institute of Biomedical Research in Cordoba (IMIBIC), Spain. Avenida Menéndez Pidal s/n, CP 14004 Córdoba, Spain
AUTHOR
Francisco
Munoz-Lopez
fcomnozz@gmail.com
5
Bio-Knowledge Lab, Glorieta de los Países Bálticos, s/n. Edificio Baobab 1, Oficina 15, Polígono Tecnocórdoba, 14014 Córdoba, Spain
AUTHOR
Alvaro
Jimenez-Arranz
genomica@imibic.org
6
Maimonides Institute of Biomedical Research in Cordoba (IMIBIC), Spain. Avenida Menéndez Pidal s/n, CP 14004 Córdoba, Spain
AUTHOR
Ipek
Guler
ipek.guler@kuleuven.be
7
Maimonides Institute of Biomedical Research in Cordoba (IMIBIC), Spain. Avenida Menéndez Pidal s/n, CP 14004 Córdoba, Spain
AUTHOR
Concha
Herrera
terapiacelular.14@gmail.com
8
Maimonides Institute of Biomedical Research in Cordoba (IMIBIC), Spain. Avenida Menéndez Pidal s/n, CP 14004 Córdoba, Spain
LEAD_AUTHOR
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ORIGINAL_ARTICLE
Effect of multi-epitope derived from HIV-1 on REM sleep deprivation-induced spatial memory impairment with respect to the level of immune factors in mice
Objective(s): Sleep deprivation (SD) has a negative impact on cognitive functions including learning and memory. Many studies have shown that rapid-eye-movement (REM) SD also disrupts memory performance. In this study, we aimed to investigate the effect of multi-epitope Gag-Pol-Env-Tat derived from Human immunodeficiency virus 1 (HIV-1) on REM SD-induced spatial memory impairment with respect to the levels of interleukin-4 (IL-4), interleukin-17 (IL-17), interferon-gamma (IFN-γ), immunoglobulin G1 (IgG1), immunoglobulin G2a (IgG2a), and lymphocyte proliferation in NMRI mice. We used multi-epitope Gag-Pol-Env-Tat derived from HIV-1 because Gag-Pol-Env-Tat immunogen sequence is one of the most sensitive immunogen sequences of HIV-1 that can significantly augment cellular and humoral immune systems, leading to the improvement of cognitive functions. Materials and Methods: Morris water maze apparatus was used to assess spatial memory, and multi-platform apparatus was used to induce RSD for 24 hr. Multi-epitope derived from HIV-1 was subcutaneously injected at the dose of 20 µgr/ml, once and fourteen days before RSD. Results: RSD impaired spatial memory and injection of multi-epitope derived from HIV-1 reversed this effect. RSD decreased IL-4, IgG1, and IgG2a levels, while multi-epitope derived from HIV-1 reversed these effects. Multi-epitope derived from HIV-1 also increased lymphocyte proliferation and decreased IL-17 levels in both control and RSD mice. Conclusion: Multi-epitope derived from HIV-1 may improve memory performance via induction of anti-inflammatory immune response.
https://ijbms.mums.ac.ir/article_19627_83bf6ffbaa06b201786ee73e21b2273d.pdf
2022-02-01
164
172
10.22038/ijbms.2022.61175.13536
Epitopes
HIV-1
Immunologic factors
Sleep deprivation
Spatial Memory
Roya
Lahimgarzadeh
rlahmi232@yahoo.com
1
Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
AUTHOR
Salar
Vaseghi
salarv67@yahoo.com
2
Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
AUTHOR
Mohammad
Nasehi
nasehi@iricss.org
3
Department of Cognitive Neuroscience, Institute for Cognitive Science Studies (ICSS), Tehran, Iran
AUTHOR
Fatemeh
Rouhollah
frohollah@iautmu.ac.ir
4
Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
LEAD_AUTHOR
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ORIGINAL_ARTICLE
Potential protective effect of astaxanthin on ovary ischemia-reperfusion injury
Objective(s): We thought that astaxanthin (ASX) might be a protective agent in oxidative stress damage that develops against ischemia and reperfusion injury in the rat ovary.Materials and Methods: The experimental groups consisted of healthy, I (Ischemia), I+ASX50, I+ASX100, I/R (Ischemia/Reperfusion), I/R+ ASX50, and I/R+ ASX100. Vascular clamps were applied to the ovaries for 3 hr to induce ischemia. For the reperfusion groups, the clamps were opened and blood flow was restored to the ovaries for 3 hr. At the end of the experiment, biochemical, histopathological, and immunohistochemical analyses were made from the tissue samples taken.Results: While MDA levels increased significantly in I and I/R groups, SOD levels decreased. It was found that ASX significantly decreased MDA levels and increased SOD activity in treatment groups depending on the dose. Caspase 3, IL-1 β, and IL-6 expressions were severely increased in ischemia and I/R groups, while the severity of I+ASX50 and I/R+ASX100 immunoreactivity was decreased. While severe hemorrhage areas were observed in I and IR groups, minimal hemorrhage areas were observed in the treatment groups, especially in I/R+ASX100 groups. In addition, inflammatory cells and necrotic cells in the I/R group were not observed in I/R+ASX50 and I/R+ASX100 groups.Conclusion: As a result, it was determined that ASX has a strong protective role against oxidative damage in the treatment of ovarian ischemia-reperfusion injury.
https://ijbms.mums.ac.ir/article_19630_52482c386086699b27dbed0c1a51c203.pdf
2022-02-01
173
178
10.22038/ijbms.2022.61289.13559
Astaxanthin
Ischemia
Ovary
Oxidative stress
Rat
Reperfusion
Erdem
Toktay
erdemtoktay@gmail.com
1
Kafkas University, Faculty of Medicine, Department of Histology and Embryology, Kars, Turkey
LEAD_AUTHOR
Tugba
Tastan
tugbabal07@gmail.com
2
Erzincan Binali Yildirim University, Faculty of Medicine, Department of Histology and Embryology, Erzincan, Turkey
AUTHOR
Muhammed
Gurbuz
mali.gurbuz1@gmail.com
3
Ataturk University, Faculty of Medicine, Department of Histology and Embryology, Erzurum, Turkey
AUTHOR
Elif
Erbas
eliferbas4154@gmail.com
4
Ataturk University, Faculty of Medicine, Department of Histology and Embryology, Erzurum, Turkey
AUTHOR
Ozlem
Demir
oz_ozlemo@hotmail.com
5
Erzincan Binali Yildirim University, Faculty of Medicine, Department of Histology and Embryology, Erzincan, Turkey
AUTHOR
Rustem
Ugan
anilugan@hotmail.com
6
Ataturk University, Faculty of Pharmacy, Department of Pharmacology, Erzurum, Turkey
AUTHOR
Jale
Selli
dr.jaleselli@hotmail.com
7
Alanya Alaaddin Keykubat University, Faculty of Medicine, Department of Histology and Embryology, Antalya, Turkey
AUTHOR
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29. Wu H, Niu H, Shao A, Wu C, Dixon BJ, Zhang J, et al. Astaxanthin as a potential neuroprotective agent for neurological diseases. Mar Drugs 2015; 13:5750-5766.
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30. Sun SQ, Zhao YX, Li SY, Qiang JW, Ji YZ. Anti-tumor effects of astaxanthin by inhibition of the expression of STAT3 in prostate cancer. Mar Drugs 2020; 18:415-428.
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31. Liu H, Liu M, Fu X, Zhang Z, Zhu L, Zheng X, et al. Astaxanthin prevents alcoholic fatty liver disease by modulating mouse gut microbiota. Nutrients 2018; 10:1298-1315.
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35. Bozkurt S, Arikan DC, Kurutas EB, Sayar H, Okumus M, Coskun A, et al. Selenium has a protective effect on ischemia/reperfusion injury in a rat ovary model: biochemical and histopathologic evaluation. J Pediatr Surg 2012; 47:1735-1741.
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36. Islam MA, Al Mamun MA, Faruk M, Ul Islam MT, Rahman MM, Alam MN, et al. Astaxanthin ameliorates hepatic damage and oxidative stress in carbon tetrachloride-administered rats. Pharmacognosy Res 2017; 9:84-91.
36
37. Topcu A, Balik G, Atak M, Mercantepe T, Uydu HA, Tumkaya L. An investigation of the effects of metformin on ovarian ischemia-reperfusion injury in rats. Eur J Pharmacol 2019; 865:172790-172800.
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39. Karacor T, Dogan Z, Elibol E, Bulbul M, Nacar MC. Effects of iloprost on experimental ischemia and reperfusion injury in rat ovary. Biotech Histochem 2020; 95:373-380.
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40. Geyikoglu F, Koc K, Erol HS, Colak S, Ayer H, Jama S, et al. The propolis and boric acid can be highly suitable, alone/or as a combinatory approach on ovary ischemia-reperfusion injury. Arch Gynecol Obstet 2019; 300:1405-1412.
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41. Carlson NG, Wieggel WA, Chen J, Bacchi A, Rogers SW, Gahring LC. Inflammatory cytokines IL-1 alpha, IL-1 beta, IL-6, and TNF-alpha impart neuroprotection to an excitotoxin through distinct pathways. J Immunol 1999; 163:3963-3968.
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43. Kim YH, Koh HK, Kim DS. Down-regulation of IL-6 production by astaxanthin via ERK-, MSK-, and NF-kappaB-mediated signals in activated microglia. Int Immunopharmacol 2010; 10:1560-1572.
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45. Cakir Gungor AN, Gencer M, Karaca T, Hacivelioglu S, Uysal A, Korkmaz F, et al. The effect of hesperetin on ischemia-reperfusion injury in rat ovary. Arch Gynecol Obstet 2014; 290:763-769.
45
ORIGINAL_ARTICLE
Crocin ameliorates MicroRNAs-associated ER stress in type 2 diabetes induced by methylglyoxal
Objective(s): Methylglyoxal (MG) provokes endoplasmic reticulum (ER) stress in β-cells and triggers pancreatic β-cell dysfunction. Crocin has anti-diabetic properties. The present study investigated whether crocin prevented pancreas damages induced by MG.Materials and Methods: Diabetes was induced by MG administration (600 mg/kg/day, PO). On the fourteenth day, after proving hyperglycemia, crocin (15, 30, and 60 mg/kg) and metformin (MT) (150 mg/kg) were used for detoxification of MG until the end of the experiment. The animals were divided into 6 groups: 1) control, 2) diabetic by MG, 3) MG + crocin 15 mg/kg, 4) MG + crocin 30 mg/kg, 5) MG + crocin 60 mg/kg, and 6) MG + MT. The data were analyzed by one-way analysis of variance and significant differences were compared by Tukey and Bonferroni tests (P<0.05). Biochemical assays, antioxidant evaluation, and microRNAs expression associated with ER stress were assessed.Results: MG induced hyperglycemia, insulin resistance, and dyslipidemia (P<0.001). Crocin and MT significantly ameliorated β-cell function through reduction of fasting blood glucose, malondialdehyde levels (P<0.001), and significant elevation of anti-oxidant enzyme activity accompanied by regulation of glutathione and glyoxalase1-Nrf2 in MG induced diabetic mice. Crocin and MT significantly down-regulated microRNAs 204, 216b, 192, and 29a expression (P<0.001). Crocin (60 mg/kg) (P<0.01) and MT (P<0.001) could improve diameter of pancreatic islets in MG treated mice. Conclusion: Crocin prevents the progression of diabetes through modulating ER stress-associated microRNAs and GLO1 activity with the helpful effects of glutathione and Nrf2.
https://ijbms.mums.ac.ir/article_19607_8ff32b78f0940a44b2d206ce49436d3f.pdf
2022-02-01
179
186
10.22038/ijbms.2022.60493.13407
Crocin
Diabetes
ER stress
Glyoxalase 1
Methylglyoxal
miR-204
Vahid
Radmehr
vahid.radmehr2@gmail.com
1
Student Research Committee, Department of Physiology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
AUTHOR
Akram
Ahangarpour
akramahangarpour@gmail.com
2
Medical Basic Sciences Research Institute, Physiology Research Center, Department of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
LEAD_AUTHOR
Seyyed Ali
Mard
alimard77@gmail.com
3
Medical Basic Sciences Research Institute, Physiology Research Center, Department of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
AUTHOR
Layasadat
Khorsandi
layasadat@yahoo.com
4
Department of Anatomical Sciences, School of Medicine, Medical Basic Sciences Research Institute, Cellular and molecular research center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
AUTHOR
1. Birgani GA, Ahangarpour A, Khorsandi L, Moghaddam HF. Anti-diabetic effect of betulinic acid on streptozotocin-nicotinamide induced diabetic male mouse model. Brazilian J Pharm Sci 2018;54:1-7.
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2. Röder P V, Wu B, Liu Y, Han W. Pancreatic regulation of glucose homeostasis. Exp Mol Med 2016;48:e219.
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3. Liu F, Zhu S, Ni L, Wang K, Zhou Y. Dexmedetomidine alleviates insulin resistance in hepatocytes by reducing endoplasmic reticulum stress. Endocrine 2020;67:87–94.
3
4. Truong C-S, Seo E, Jun H-S. Psoralea corylifolia L. seed extract attenuates methylglyoxal-induced insulin resistance by inhibition of advanced glycation end product formation. Oxid Med Cell Longev 2019;2019.
4
5. Liu C, Huang Y, Zhang Y, Chen X, Kong X, Dong Y. Intracellular methylglyoxal induces oxidative damage to pancreatic beta cell line INS-1 cell through Ire1α-JNK and mitochondrial apoptotic pathway. Free Radic Res 2017;51:337–350.
5
6. Suantawee T, Thilavech T, Cheng H, Adisakwattana S. Cyanidin Attenuates Methylglyoxal-Induced Oxidative Stress and Apoptosis in INS-1 Pancreatic β-Cells by Increasing Glyoxalase-1 Activity. Nutrients. 2020;12:1-12.
6
7. Cnop M, Toivonen S, Igoillo-Esteve M, Salpea P. Endoplasmic reticulum stress and eIF2α phosphorylation: The Achilles heel of pancreatic β cells. Mol Metab 2017;6:1024–1039.
7
8. Chen Y-J, Kong L, Tang Z-Z, Zhang Y-M, Liu Y, Wang T-Y, et al. Hesperetin ameliorates diabetic nephropathy in rats by activating Nrf2/ARE/glyoxalase 1 pathway. Biomed Pharmacother 2019;111:1166–1175.
8
9. Xue M, Rabbani N, Momiji H, Imbasi P, Anwar MM, Kitteringham N, et al. Transcriptional control of glyoxalase 1 by Nrf2 provides a stress-responsive defence against dicarbonyl glycation. Biochem J 2012;443:213–222.
9
10. Liu Y-W, Liu X-L, Kong L, Zhang M-Y, Chen Y-J, Zhu X, et al. Neuroprotection of quercetin on central neurons against chronic high glucose through enhancement of Nrf2/ARE/glyoxalase-1 pathway mediated by phosphorylation regulation. Biomed Pharmacother 2019;109:2145–2154.
10
11. Rabbani N, Thornalley PJ. The critical role of methylglyoxal and glyoxalase 1 in diabetic nephropathy. Diabetes. 2014;63:50–52.
11
12. Li Y, Qian J, Yang L. Inhibition of YBX1 by miR-216a Suppresses Proliferation and Invasion of Diffuse Large B-Cell Lymphoma. Balkan Med J 2021;38:171-176.
12
13. Grieco FA, Schiavo AA, Brozzi F, Juan-Mateu J, Bugliani M, Marchetti P, et al. The miRNAs miR-211-5p and miR-204-5p modulate ER stress in human beta cells. J Mol Endocrinol 2019;63:139–149.
13
14. Endo K, Weng H, Kito N, Fukushima Y, Iwai N. MiR-216a and miR-216b as markers for acute phased pancreatic injury. Biomed Res 2013;34:179–188.
14
15. Pan W, Zhang Y, Zeng C, Xu F, Yan J, Weng J. miR‑192 is upregulated in T1DM, regulates pancreatic β‑cell development and inhibits insulin secretion through suppressing GLP‑1 expression. Exp Ther Med 2018;16:2717–2724.
15
16. Dujic T, Zhou K, Donnelly LA, Tavendale R, Palmer CNA, Pearson ER. Association of organic cation transporter 1 with intolerance to metformin in type 2 diabetes: a GoDARTS study. Diabetes 2015;64:1786–1793.
16
17. Wang C, Cai X, Hu W, Li Z, Kong F, Chen X, et al. Investigation of the neuroprotective effects of crocin via anti-oxidant activities in HT22 cells and in mice with Alzheimer’s disease. Int J Mol Med 2019;43:956–966.
17
18. Erdemli M, Erdemli Z, Gul M, Altinoz E, Gul S, Kocaman G, et al. Ameliorative effects of crocin on the inflammation and oxidative stress-induced kidney damages by experimental periodontitis in rat. Iran J Basic Med Sci 2021;24:825–832.
18
19. Algandaby MM. Antifibrotic effects of crocin on thioacetamide-induced liver fibrosis in mice. Saudi J Biol Sci 2018;25:747–754.
19
20. Korani S, Korani M, Sathyapalan T, Sahebkar A. Therapeutic effects of Crocin in autoimmune diseases: A review. BioFactors 2019;45:835–843.
20
21. Ahmed S, Hasan MM, Heydari M, Rauf A, Bawazeer S, Abu-Izneid T, et al. Therapeutic potentials of crocin in medication of neurological disorders. Food Chem Toxicol 2020;145:111739.
21
22. Salama RM, Abdel-Latif GA, Abbas SS, Hekmat M, Schaalan MF. Neuroprotective effect of crocin against rotenone-induced Parkinson’s disease in rats: Interplay between PI3K/Akt/mTOR signaling pathway and enhanced expression of miRNA-7 and miRNA-221. Neuropharmacology 2020;164:107900.
22
23. Arai M, Nihonmatsu-Kikuchi N, Itokawa M, Rabbani N, Thornalley PJ (2014) Measurement of glyoxalase activities. Biochem Soc Trans 2014; 42:491–494.
23
24. Lee B-H, Hsu W-H, Chang Y-Y, Kuo H-F, Hsu Y-W, Pan T-M. Ankaflavin: a natural novel PPARγ agonist upregulates Nrf2 to attenuate methylglyoxal-induced diabetes in vivo. Free Radic Biol Med 2012;53:2008–2016.
24
25. Mojadami S, Ahangarpour A, Mard SA, Khorsandi L. Diabetic nephropathy induced by methylglyoxal: gallic acid regulates kidney microRNAs and glyoxalase1–Nrf2 in male mice. Arch Physiol Biochem 2021;1–8.
25
26. Qi SS, Zheng HX, Jiang H, Yuan LP, Dong LC. Protective effects of chromium picolinate against diabetic-induced renal dysfunction and renal fibrosis in streptozotocin-induced diabetic rats. Biomolecules 2020;10:1-13.
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27
28. Chang W-C, Wu S-C, Xu K-D, Liao B-C, Wu J-F, Cheng A-S. Scopoletin protects against methylglyoxal-induced hyperglycemia and insulin resistance mediated by suppression of advanced glycation endproducts (AGEs) generation and anti-glycation. Molecules 2015;20:2786–2801.
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29. Vanessa Fiorentino T, Prioletta A, Zuo P, Folli F. Hyperglycemia-induced oxidative stress and its role in diabetes mellitus related cardiovascular diseases. Curr Pharm Des 2013;19:5695–5703.
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30. Burgos-Morón E, Abad-Jiménez Z, Martinez de Maranon A, Iannantuoni F, Escribano-López I, López-Domènech S, et al. Relationship between oxidative stress, ER stress, and inflammation in type 2 diabetes: the battle continues. J Clin Med 2019;8:1-22.
30
31. Suh KS, Chon S, Jung W-W, Choi EM. Magnolol protects pancreatic β-cells against methylglyoxal-induced cellular dysfunction. Chem Biol Interact 2017;277:101–109.
31
32. Schumacher D, Morgenstern J, Oguchi Y, Volk N, Kopf S, Groener JB, et al. Compensatory mechanisms for methylglyoxal detoxification in experimental & clinical diabetes. Mol Metab 2018;18:143–152.
32
33. Delle Monache S, Pulcini F, Frosini R, Mattei V, Talesa VN, Antognelli C. Methylglyoxal-dependent glycative stress is prevented by the natural anti-oxidant oleuropein in human Dental pulp stem cells through Nrf2/Glo1 pathway. Anti-oxidants. 2021;10:1-19.
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34. Rodrigues T, Matafome P, Sereno J, Almeida J, Castelhano J, Gamas L, et al. Methylglyoxal-induced glycation changes adipose tissue vascular architecture, flow and expansion, leading to insulin resistance. Sci Rep 2017;7:1–13.
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35. Filippatos T, Tsimihodimos V, Pappa E, Elisaf M. Pathophysiology of diabetic dyslipidaemia. Curr Vasc Pharmacol 2017;15:566–575.
35
36. Shirali S, Zahra Bathaie S, Nakhjavani M. Effect of crocin on the insulin resistance and lipid profile of streptozotocin‐induced diabetic rats. Phyther Res 2013;27:1042–1047.
36
37. Bagge A, Clausen TR, Larsen S, Ladefoged M, Rosenstierne MW, Larsen L, et al. MicroRNA-29a is up-regulated in beta-cells by glucose and decreases glucose-stimulated insulin secretion. Biochem Biophys Res Commun 2012;426:266–272.
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38. Moi P, Chan K, Asunis I, Cao A, Kan YW. Isolation of NF-E2-related factor 2 (Nrf2), a NF-E2-like basic leucine zipper transcriptional activator that binds to the tandem NF-E2/AP1 repeat of the beta-globin locus control region. Proc Natl Acad Sci 1994;91:9926–9930.
38
39. Kassan M, Vikram A, Kim Y-R, Li Q, Kassan A, Patel HH, et al. Sirtuin1 protects endothelial Caveolin-1 expression and preserves endothelial function via suppressing miR-204 and endoplasmic reticulum stress. Sci Rep 2017;7:1–10.
39
40. Xu Z, Bu Y, Chitnis N, Koumenis C, Fuchs SY, Diehl JA. miR-216b regulation of c-Jun mediates GADD153/CHOP-dependent apoptosis. Nat Commun 2016;7:1–12.
40
ORIGINAL_ARTICLE
Anti-tumor activity of a recombinant soluble Fzd7 decoy receptor in human gastric and colon cancer cells
Objective(s): Frizzled-7, the most common receptor of the Wnt signaling pathway, was significantly over-expressed in gastric (GC) and colorectal (CRC) cancers and stimulated tumorigenesis. The extracellular domain of Fzd7 (sFzd7) as a decoy receptor, could competitively bound with ligands and antagonize the interaction between Fzd7 receptors and Wnt ligands. Materials and Methods: We expressed and purified the extracellular region of Fzd7 including cysteine-rich domain (33 aa–185 aa) from Escherichia coli by chromatography. The effect of sFzd7 was evaluated on AGS gastric and SW480 colon cancer cell lines expressing high levels of Fzd7 receptor. Accordingly, cell viability and apoptosis were measured using MTT and flow cytometry assays, respectively. Real-Time PCR determined the relative expression of the β-catenin and cyclin-D1 genes. Results: After three days of treatment with sFzd7, the viability of AGS and SW480 cell lines was decreased in a dose-dependent manner. In addition, sFzd7 at concentrations of 10 and 20 ug/ml increased the rate of apoptosis. Especially at the concentration of 20 ug/ml, the apoptosis rate was remarkably high in AGS (P-value= 0.003) and SW480 cells (P-value= 0.0007). Finally, the expressions of β-catenin (P-value= 0.01) and cyclin-D1 (P-value= 0.02) were obviously decreased in SW480 cells. The same results were obtained in AGS cells, although not statistically significant. Conclusion: sFzd7 decoy receptor inhibits tumor cell progression by attenuating the Wnt pathway through inhibiting Fzd7 receptors and Wnt ligand interaction. Hence, sFzd7 can be proposed as a candidate therapy for GC and CRC cells with high levels of Fzd7 expression.
https://ijbms.mums.ac.ir/article_19608_c1d94b0754f52f6e2119a18b16e9eb98.pdf
2022-02-01
187
192
10.22038/ijbms.2022.61908.13700
Anti-tumor activity
Colon cancer
Decoy receptor
Gastric cancer
Recombinant protein
Soluble Fzd7
Nasim
Hafezi
nsm.hafezi@gmail.com
1
Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
AUTHOR
Reza
Valadan
valadan.reza@gmail.com
2
Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
AUTHOR
Hossein
Asgarian-Omran
asgarianhossein@yahoo.com
3
Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
AUTHOR
Abolghasem
Ajami
ajami36@gmail.com
4
Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
LEAD_AUTHOR
1. Arnold M, Abnet CC, Neale RE, Vignat J, Giovannucci EL, McGlynn KA, et al. Global burden of 5 major types of gastrointestinal cancer. Gastroenterology 2020; 159:335-349. e315.
1
2. Thrift AP, El-Serag HB. Burden of gastric cancer. Clin Gastroenterol Hepatol 2020; 18:534-542.
2
3. Wang L-Y, Zhao S, Lv G-J, Ma X-J, Zhang J-B. Mechanisms of resveratrol in the prevention and treatment of gastrointestinal cancer. World J. Clin. Cases 2020; 8:2425-2437.
3
4. Kim MJ, Huang Y, Park J-I. Targeting wnt signaling for gastrointestinal cancer therapy: Present and evolving views. Cancers 2020; 12:3638-3665.
4
5. Zhan T, Rindtorff N, Boutros M. Wnt signaling in cancer. Oncogene 2017; 36:1461-1473.
5
6. Sherwood V. WNT signaling: an emerging mediator of cancer cell metabolism? Mol Cell Biol 2015; 35:2-10.
6
7. Dijksterhuis JP, Baljinnyam B, Stanger K, Sercan HO, Ji Y, Andres O, et al. Systematic mapping of WNT-FZD protein interactions reveals functional selectivity by distinct WNT-FZD pairs. J Biol Chem 2015; 290:6789-6798.
7
8. Phesse T, Flanagan D, Vincan E. Frizzled7: a promising Achilles’ heel for targeting the Wnt receptor complex to treat cancer. Cancers 2016; 8:50-82.
8
9. Li G, Su Q, Liu H, Wang D, Zhang W, Lu Z, et al. Frizzled7 promotes epithelial-to-mesenchymal transition and stemness via activating canonical Wnt/β-catenin pathway in gastric cancer. Int J Biol Sci 2018; 14:280-293.
9
10. Jothimani M, Loganathan L, Palanisamy P, Muthusamy K. Regulatory pathways of colorectal cancer and their synergistic cross-talk mechanism. Ann colorectal res 2020; 8:105-119.
10
11. Ye C, Xu M, Lin M, Zhang Y, Zheng X, Sun Y, et al. Overexpression of FZD7 is associated with poor survival in patients with colon cancer. Pathol Res Pract 2019; 215:152478.
11
12. Cheng Y, Li L, Pan S, Jiang H, Jin H. Targeting frizzled-7 decreases stemness and chemotherapeutic resistance in gastric cancer cells by suppressing Myc expression. Med Sci Monit 2019; 25:8637-8644.
12
13. Ueno K, Hazama S, Mitomori S, Nishioka M, Suehiro Y, Hirata H, et al. Down-regulation of frizzled-7 expression decreases survival, invasion and metastatic capabilities of colon cancer cells. Br J Cancer 2009; 101:1374-1381.
13
14. Xie W, Zhang Y, He Y, Zhang K, Wan G, Huang Y, et al. A novel recombinant human Frizzled-7 protein exhibits anti-tumor activity against triple negative breast cancer via abating Wnt/β-catenin pathway. Int J Biochem Cell Biol 2018; 103:45-55.
14
15. Schmuck R, Warneke V, Behrens H-M, Simon E, Weichert W, Röcken C. Genotypic and phenotypic characterization of side population of gastric cancer cell lines. Am J Clin Pathol 2011; 178:1792-1804.
15
16. Ueno K, Hiura M, Suehiro Y, Hazama S, Hirata H, Oka M, et al. Frizzled-7 as a potential therapeutic target in colorectal cancer. Neoplasia 2008; 10:697-705.
16
17. Konner J, Dupont J. Use of soluble recombinant decoy receptor vascular endothelial growth factor trap (VEGF Trap) to inhibit vascular endothelial growth factor activity. Clin Colorectal Cancer 2004; 4:S81-S85.
17
18. de Moura PR, Watanabe L, Bleicher L, Colau D, Dumoutier L, Lemaire MM, et al. Crystal structure of a soluble decoy receptor IL-22BP bound to interleukin-22. FEBS Lett 2009; 583:1072-1077.
18
19. Itatani Y, Kawada K, Yamamoto T, Sakai Y. Resistance to anti-angiogenic therapy in cancer—alterations to anti-VEGF pathway. Int J Mol Sci 2018; 19:1232-1249.
19
20. Ahmadzadeh M, Farshdari F, Nematollahi L, Behdani M, Mohit E. Anti-HER2 scFv expression in Escherichia coli SHuffle® T7 express cells: effects on solubility and biological activity. Mol Biotechnol 2020; 62:18-30.
20
21. Li Y-J, Wei Z-M, Meng Y-X, Ji X-R. β-catenin up-regulates the expression of cyclinD1, c-myc and MMP-7 in human pancreatic cancer: relationships with carcinogenesis and metastasis. World J Gastroenterol 2005; 11:2117-2123.
21
22. Marcolino TF, Pimenta CAM, Neto RA, Castelo P, Silva MS, Forones NM, et al. p53, Cyclin-D1, β-catenin, APC and c-myc in tumor tissue from colorectal and gastric cancer patients with suspected Lynch syndrome by the Bethesda criteria. APJCP 2020; 21:343.
22
23. Ripple MJ, Parker Struckhoff A, Trillo-Tinoco J, Li L, Margolin DA, McGoey R, et al. Activation of c-Myc and Cyclin D1 by JCV T-Antigen and β-catenin in colon cancer. PLoS One 2014; 9:e106257.
23
24. DeAlmeida VI, Miao L, Ernst JA, Koeppen H, Polakis P, Rubinfeld B. The soluble wnt receptor Frizzled8CRD-hFc inhibits the growth of teratocarcinomas in vivo. Cancer Res 2007; 67:5371-5379.
24
25. Wei W, Chua M-S, Grepper S, So SK. Soluble Frizzled-7 receptor inhibits Wnt signaling and sensitizes hepatocellular carcinoma cells towards doxorubicin. Mol Cancer 2011; 10:1-12.
25
ORIGINAL_ARTICLE
Crocin protects the renal tubular epithelial cells against high glucose-induced injury and oxidative stress via regulation of the SIRT1/Nrf2 pathway
Objective(s): Renal tubular damage is critical pathological feathers of diabetic nephropathy (DN). This study aimed to explore the protective activity and related mechanisms of crocin in renal epithelial cell injury induced by high glucose.Materials and Methods: Renal tubular epithelial HK-2 cells were cultured with D-glucose to establish an in vitro DN model. Cell viability was evaluated by CCK-8 assay. Apoptosis was detected by Annexin V-FITC kit. Oxidative stress was evaluated by colorimetry. RT-qPCR was carried out to determine the mRNA expressions of NF-E2-related factor 2 (Nrf2) and its pathway genes. Western blot was applied to determine the protein expressions of Nrf2 and related proteins.Results: High glucose (5.5, 30, and 50 mM D-glucose) decreased cell viability at 72 hr, which was attenuated by crocin (25 and 50 μM). Crocin also attenuated the high glucose (30 mM D-glucose) induced apoptosis of HK-2 cells, decreased MDA content, and increased SOD activity in culture media. Crocin increased mRNA levels of Nrf2, HO-1, and NQO1. Moreover, crocin increased protein expressions of Nrf2, Sirtuin 1 (SIRT1), and p-Akt (Ser473). Inhibition of Nrf2 using siRNA, and inhibitors of SIRT1 (nicotinamide, NAM, 20 μM) and PI3K/Akt (LY294002, 50 μM) all attenuated the protective effect of crocin. Nrf2 siRNA and NAM also partially attenuated the inhibitory effect on oxidative stress and increase in the Nrf2 protein by crocin treatment.Conclusion: Crocin protects renal epithelial cells against injury induced by high glucose, and the mechanism is associated with partial activation of the SIRT1-Nrf2 pathway.
https://ijbms.mums.ac.ir/article_19606_62fd7caaff7c6c71041eaf13574c0e82.pdf
2022-02-01
193
197
10.22038/ijbms.2022.51597.11708
Diabetic nephropathy
High glucose
NF-E2-related factor 2
Oxidative stress
Renal tubular epithelial cell
Sirtuin 1
Jichen
Zhang
zjc000_0@126.com
1
Department of Endocrinology, Shanghai Pudong New District Gongli Hospital, Second Military Medical University, Shanghai 200135, P. R China
AUTHOR
Xuemei
Zhao
13955584826@163.com
2
Department of Endocrinology, Shanghai Pudong New District Gongli Hospital, Second Military Medical University, Shanghai 200135, P. R China
AUTHOR
Hongling
Zhu
13671550991@163.com
3
Department of Endocrinology, Shanghai Pudong New District Gongli Hospital, Second Military Medical University, Shanghai 200135, P. R China
AUTHOR
Jingnan
Wang
jingnan_wang@163.com
4
Department of Endocrinology, Shanghai Pudong New District Gongli Hospital, Second Military Medical University, Shanghai 200135, P. R China
AUTHOR
Junhua
Ma
majunhua821120@126.com
5
Department of Endocrinology, Shanghai Pudong New District Gongli Hospital, Second Military Medical University, Shanghai 200135, P. R China
AUTHOR
Mingjun
Gu
gumjsh@163.com
6
Department of Endocrinology, Shanghai Pudong New District Gongli Hospital, Second Military Medical University, Shanghai 200135, P. R China
LEAD_AUTHOR
1. Waanders F, Visser FW, Gans RO. Current concepts in the management of diabetic nephropathy. Neth J Med 2013; 71: 448-458.
1
2.Kanwar YS, Wada J, Sun L, Xie P, Wallner EI, Chen S, et al. Diabetic nephropathy: mechanisms of renal disease progression. Exp Biol Med (Maywood) 2008; 233: 4-11.
2
3. Wu CZ, Chang LC, Lin YF, Hung YJ, Pei D, Chen JS. Chaperonin-containing t-complex protein-1 subunit β as a possible biomarker for the phase of glomerular hyperfiltration of diabetic nephropathy. Dis Markers 2015;2015:548101.
3
4. Yuan Y, Sun H, Sun Z. Advanced glycation end products (AGEs) increase renal lipid accumulation: a pathogenic factor of diabetic nephropathy (DN). Lipids Health Dis 2017;16:126.
4
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33
ORIGINAL_ARTICLE
Effect of alpha-mangostin on olanzapine-induced metabolic disorders in rats
Objective(s): As olanzapine has side effects such as weight gain and metabolic disorders, and alpha-mangostin has been shown to control metabolic disorders, the effects of alpha-mangostin on metabolic disorders induced by olanzapine were investigated in this study.Materials and Methods: Obesity was induced in female Wistar rats by daily administration of olanzapine (5 mg/kg/day, IP, 14 days). Rats were divided into 6 groups:1) vehicle (control); 2) olanzapine (5 mg/kg/day); 3,4,5) olanzapine+ alpha-mangostin (10, 20, 40 mg/kg/day, IP); 6) alpha-mangostin (40 mg/kg/day). Weight changes were measured every 3 days and food intake was assessed every day. Systolic blood pressure, plasma levels of blood sugar, triglycerides, total cholesterol, HDL, LDL, leptin, oxidative stress markers (MDA, GSH), AMPK, and P-AMPK protein levels in liver tissue were assessed on the last day of the study. Results: Administration of olanzapine significantly increased weight gain, food intake, blood pressure, triglycerides, LDL, blood sugar, leptin, and MDA in rat liver tissue and also decreased GSH, AMPK, and P-AMPK in liver tissue compared with the control group. Different doses of alpha-mangostin significantly reduced weight gain, food intake, systolic blood pressure, triglycerides, LDL, blood sugar, leptin, and MDA. Also, they significantly increased GSH, AMPK, and P-AMPK in liver tissue compared with the olanzapine group.Conclusion: Olanzapine increases leptin levels, food intake, and weight, induces oxidative stress, decreases the levels of AMPK and P-AMPK proteins in liver tissue, and causes metabolic disorders. But, alpha-mangostin reduces the negative effects of olanzapine by activation of AMPK.
https://ijbms.mums.ac.ir/article_19581_143c4003b1023d5e0ede96120dcf99ef.pdf
2022-02-01
198
207
10.22038/ijbms.2022.58734.13047
Anti-Oxidants
Leptin
Liver
Mangostin
metabolic syndrome
Obesity
Olanzapine
Weight gain
Alireza
Ardakanian
alirezaard984@gmail.com
1
Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Mahboobeh
Ghasemzadeh Rahbardar
ghasemzadeh_mahboobeh@yahoo.com
2
Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Farzaneh
Omidkhoda
omidkhodaf9@mums.ac.ir
3
Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Bibi Marjan
Razavi
razavimr@mums.ac.ir
4
Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Hossein
Hosseinzadeh
hosseinzadehh@mums.ac.ir
5
Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
LEAD_AUTHOR
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69
ORIGINAL_ARTICLE
Molecular typing of clinical and environmental isolates of Klebsiella pneumoniae producing ESBLs by PFGE
Objective(s): Klebsiella pneumoniae is the common cause of pneumonia in hospitalized patients, particularly in intensive care units (ICU). The infection can transfer by medical equipment such as mechanical ventilators. This study aimed to investigate the molecular typing of the extended-spectrum beta-lactamase-producing K. pneumoniae isolates recovered from Beheshti Hospital, Kashan, Iran. Materials and Methods: K. pneumoniae isolates producing ESBLs have been collected from the samples obtained from Shahid Beheshti hospital, Kashan, Iran. Antimicrobial susceptibility was determined using the Kirby Bauer disk diffusion method. The presence of ESBLs was evaluated using CLSI for ESBL screening by the double-disk diffusion method. Molecular typing was conducted by pulsed-field gel electrophoresis (PFGE). In total, 89 K. pneumoniae isolates were recovered, of which 47.1% were ESBL producers.Results: Results showed that all of the clinical and environmental isolates were resistant to ceftriaxone, meropenem, cefazolin, cefotaxime, cephalothin, and piperacillin-tazobactam. All isolates were grouped under four clusters (A-D). The major cluster was related to the C cluster with 22 isolates (19 clinical and 3 environmental). Seventy-two percent of isolates were from the ICU ward. There was no correlation between antibiotic resistance patterns and PFGE clusters (P=0.2).Conclusion: We observed a common molecular signature among both clinical and environmental K. pneumoniae isolates, indicating a similar genotype and likely a common origin for ESBL producer isolates found in different hospital wards. Therefore, hospitals need to implement an effective infection control system to decrease the spreading of ESBL strains within the hospitals and subsequently the transmission of the infection to patients.
https://ijbms.mums.ac.ir/article_19582_4439b79e901c505ddec400468f0fac71.pdf
2022-02-01
208
213
10.22038/ijbms.2022.58445.12981
Antibiotic resistance
ICU
Infection
Iran
Medical equipment
Mohammad
Esmaeilnia
mo4esm2@gmail.com
1
Department of Microbiology and Immunology, Faculty of Medicine, Kashan University of Medical Science, Kashan, Iran
AUTHOR
Mahmood
Saffari
safari89.90mah@yahoo.com
2
Department of Microbiology and Immunology, Faculty of Medicine, Kashan University of Medical Science, Kashan, Iran
AUTHOR
Somaye
Rashki
rashk89som90@yahoo.com
3
Department of Microbiology and Immunology, Faculty of Medicine, Kashan University of Medical Science, Kashan, Iran
AUTHOR
Zeynab
Marzhoseyni
zaynab.90marz90@yahoo.com
4
Department of Microbiology and Immunology, Faculty of Medicine, Kashan University of Medical Science, Kashan, Iran
AUTHOR
Azad
Khaledi
azadkh99@gmail.com
5
Department of Microbiology and Immunology, Faculty of Medicine, Kashan University of Medical Science, Kashan, Iran
LEAD_AUTHOR
Gholam Abbas
Moosavi
ghol90abas45@yahoo.com
6
Department of Vital Statistics and Epidemiology, School of Health, Kashan University of Medical Sciences, Kashan, Iran
AUTHOR
Fatemeh
Atoof
fatem89.atoo89@yahoo.com
7
Department of Biostatistics, Health Faculty, Kashan University of Medical Sciences, Kashan, Iran
AUTHOR
Behrang
Alani
behrang90alani2@yahoo.com
8
Department of Applied Cell Sciences, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran
AUTHOR
1. Gharavi MJ, Zarei J, Roshani-Asl P, Yazdanyar Z, Sharif M, Rashidi N. Comprehensive study of antimicrobial susceptibility pattern and extended spectrum beta-lactamase (ESBL) prevalence in bacteria isolated from urine samples. Sci Rep 2021;11:1-11.
1
2. Guo Y, Zhou H, Qin L, Pang Z, Qin T, Ren H, et al. Frequency, antimicrobial resistance and genetic diversity of Klebsiella pneumoniae in food samples. PLoS One 2016;11:e0153561.
2
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37
ORIGINAL_ARTICLE
Protective and therapeutic role of melatonin against tunicamycin-induced ER stress in testicular tissue of rats
Objective(s): This study aimed to investigate the possible consequences of administering exogenous melatonin as prevention or treatment against tunicamycin-induced endoplasmic reticulum (ER) stress in the testicular tissue of rats. Materials and Methods: In this study, 42 adult Sprague Dawley rats, randomly divided into seven equal groups, were administered intraperitoneal tunicamycin to induce ER stress. Both prophylactic (PMel) and therapeutic melatonin (TMel) groups were administered melatonin for seven days. ER stress in the cell was detected through immunohistochemical and molecular analyses using GPR78 expression. Results: Increased oxidant levels and apoptosis rates were shown in testicular tissue because of ER stress. The sections in the melatonin-administered and control groups were similar, with melatonin-administered groups showing an increase in the antioxidant ratio. Histometric examinations revealed both TMel and melatonin applications reduced the diameter of the tubules. However, immunohistochemical and molecular analyses showed that PMel administration decreased the concentration of GRP78 more effectively than TMel. Conclusion: Applying melatonin prior to cell damage occurrence can be recommended for its effectiveness in protecting from tunicamycin-induced ER stress.
https://ijbms.mums.ac.ir/article_19590_64d696127b9f2ea3b00562b23eec9a40.pdf
2022-02-01
214
222
10.22038/ijbms.2022.58719.13043
Apoptosis
Endoplasmic reticulum stress
Endoplasmic reticulum chaperone BiP
Melatonin
Testis
Tunicamycin
Musa
Tatar
musatatar88@hotmail.com
1
Department of Histology and Embryology, Faculty of Veterinary Medicine, Kastamonu University, Kastamonu, Turkey
LEAD_AUTHOR
Ülker
Eren
ueren@adu.edu.tr
2
Department of Histology and Embryology, Faculty of Veterinary Medicine, Aydın Adnan Menderes University, Aydin, Turkey
AUTHOR
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59
ORIGINAL_ARTICLE
A novel nanomicelle composed from PEGylated TB di-peptide could be successfully used as a BCG booster
Objective(s): Tuberculosis affects one-third of the world’s population and leads to a high rate of morbidity and mortality. Bacillus Chalmette–Guerin (BCG) as the only approved vaccine for the Mycobacterium tuberculosis (Mtb) does not show enough protection in the vaccinated population. Materials and Methods: The main aim of this study was to prepare a self-assembled nanomicelle composed from a di-block polymer in which, a di-fusion peptide was the hydrophobic block and polyethylene glycol (PEG) was the hydrophilic block. The micelles were characterized in vitro and in vivo as an antigen delivery system/adjuvant both with and without a prime BCG. Results: The micellar nanovaccine was able to elicit good dendritic cell maturation. Nanomicelles could efficiently induce systemic cytokines as well as nasal secretory predominant antibody titers (sIgA). The expression pattern of cytokines indicated the superiority of cellular immunity. Nasal administration of two doses of nanomicelles after a prime subcutaneous administration of BCG induced the highest mucosal and systemic immune responses. Conclusion: Based on our results PEG-HspX/EsxS self-assembled nanomicelle is highly immunogenic and can be considered a potential vaccine candidate against Mtb to boost BCG efficiency.
https://ijbms.mums.ac.ir/article_19657_9829f91e429d0067e00b7b08914cd87b.pdf
2022-02-01
223
231
10.22038/ijbms.2022.61373.13583
Nanovaccine
Nasal and parenteral immunization
Recombinant fusion peptide
Self-assembled nanomicelles
Tuberculosis
Zohreh
Firouzi
zohre.firouzi@gmail.com
1
Department of Pharmaceutical Nanotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Mahmoud
Jaafari
jaafarim@mums.ac.ir
2
Department of Pharmaceutical Nanotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Mojtaba
Sankian
sankianm@mums.ac.ir
3
Immunobiochemistry Laboratory, Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Sirwan
Zare
zares@mums.ac.ir
4
Immunobiochemistry Laboratory, Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
Mohsen
Tafaghodi
tafaghodim@mums.ac.ir
5
Department of Pharmaceutical Nanotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
LEAD_AUTHOR
1. Gröschel MN, Prabowo SA, Cardona PJ, Stanford JL, and van der Werf TS., Therapeutic vaccines for tuberculosis--a systematic review. Vaccine 2014; 32: 3162-3168.
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3
4. Brandt L, Cunha JF, Olsen AW, Chilima B, Hirsch P, Appelberg R, Andersen P. Failure of the Mycobacterium bovis BCG vaccine: Some species of environmental mycobacteria block multiplication of BCG and induction of protective immunity to tuberculosis. Infec Immun 2002; 70: 672-678.
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14. Khademi F, Yousefi-Avarvand A, Derakhshan M, Abbaspour MR, Sadri K, Tafaghodi M. Formulation and optimization of a new cationic lipid-modified PLGA nanoparticle as delivery system for Mycobacterium tuberculosis HspX/EsxS fusion protein: An experimental design. IJPR 2019; 18: 446–458.
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21. Yazdani M, Gholizadeh Z, Nikpoor AR, Hatamipour M, Alani B, Nikzad H, et al. Vaccination with dendritic cells pulsed ex vivo with gp100 peptide-decorated liposomes enhances the efficacy of anti PD-1 therapy in a mouse model of melanoma. Vaccine 2020. 38:5665-5677.
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22. Khademi F, Derakhshan M, Avarvand AY, Najafi N, Tafaghodi M. Multi-stage subunit vaccines against Mycobacterium tuberculosis: An alternative to the BCG vaccine or a BCG-prime boost? Expert Rev Vaccines 2018; 17:31-44.
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26. Mansury D, Ghazvini K, Jamehdar SA, Badiee A, Tafaghodi M, Nikpoor AR, et al. Enhancement of the effect of BCG vaccine against tuberculosis using DDA/TDB liposomes containing a fusion protein of HspX, PPE44, and EsxV. Artif Cells Nanomed Biotechnol 2019; 47: 370–377.
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46
ORIGINAL_ARTICLE
Ib-AMP4 antimicrobial peptide as a treatment for skin and systematic infection of methicillin-resistant Staphylococcus aureus (MRSA)
Objective(s): Antimicrobial peptide compounds (AMPs) play important roles in the immune system. They also exhibit significant anti-tumor and antibacterial properties. Most AMPs are cationic and are able to bind bacterial cell membranes through electrostatic affinity. Ib-AMP4 is a plant-derived AMP that exerts rapid bactericidal functions. In the present study, the antibacterial efficiency of the produced recombinant Ib-AMP4 in elimination of Methicillin-resistant Staphylococcus aureus (MRSA) bacterial infection, was investigated under in vitro and in vivo situations. Materials and Methods: The synthesized Escherichia coli codon-optimized gene sequences of the Ib-AMP4 were expressed in E. coli BL21 (DE3) pLysS. The recombinant Ib-AMP4 was purified and refolding conditions were optimized. The antibacterial efficiency of the refolded peptide against MRSA was tested under in vivo and in vitro situations for treatment of skin and systematic infection of MRSA in a mouse model.Results: Antibacterial assays confirmed the antibacterial function of Ib-AMP4 against MRSA. SEM results proved the destructive effects of applying Ib-AMP4 on MRSA biomembrane. Time-kill curve and growth kinetic assay illustrated rapid antibacterial activity of the produced Ib-AMP4. Moreover, Ib-AMP4 showed significant infection treatment ability in a mouse model and all infected mice receiving Ib-AMP4 protein survived and there was no trace of bacteria in their blood samples.Conclusion: The results confirmed the rapid antibacterial potential of the produced recombinant Ib-AMP4 to be used for efficient treatment of MRSA infection.
https://ijbms.mums.ac.ir/article_19658_8c2fdfc71a5fe4dc101f19dc83804ecd.pdf
2022-02-01
232
238
10.22038/ijbms.2022.61043.13508
Antimicrobial activity
Recombinant Ib-AMP4
Skin and systematic infection
Staphylococcus aureus
Samira
Sadelaji
samirasadelaji@yahoo.com
1
Molecular and Medical Research Center, Arak University of Medical Sciences, Arak, Iran
AUTHOR
Ehsanollah
Ghaznavi-Rad
ghaznaviehs@yahoo.com
2
Molecular and Medical Research Center, Arak University of Medical Sciences, Arak, Iran
AUTHOR
Shabnam
Sadoogh Abbasian
shabmicro@yahoo.com
3
Molecular and Medical Research Center, Arak University of Medical Sciences, Arak, Iran
AUTHOR
Shohreh
Fahimirad
sh_fahimirad@yahoo.com
4
Molecular and Medical Research Center, Arak University of Medical Sciences, Arak, Iran
AUTHOR
Hamid
Abtahi
habtahi2001@yahoo.com
5
Molecular and Medical Research Center, Arak University of Medical Sciences, Arak, Iran
LEAD_AUTHOR
1. Avison MB. New approaches to combating antimicrobial drug resistance. Genome Biol 2005;6:243-246.
1
2. Sarkar T, Chetia M & Chatterjee, S. Antimicrobial peptides and proteins: From nature’s reservoir to the laboratory and beyond. Front Chem 2021;9:691532.
2
3. Mukhopadhyay S, Bharath Prasad AS, Mehta CH & Nayak UY. Antimicrobial peptide polymers: No escape to ESKAPE pathogens-a review. World J Microbiol Biotechnol 2020;36:131-144.
3
4. Flórez-Castillo JM, Ropero-Vega JL, Perullini M, & Jobbágy M. Biopolymeric pellets of polyvinyl alcohol and alginate for the encapsulation of Ib-M6 peptide and its antimicrobial activity against Escherichia coli. Heliyon 2019; 5: e01872.
4
5. Sadoogh Abbasian S, Abtahi H, Zolfaghari MR, Soufian S and Ghaznavi-Rad E. Cloning, expression,purification and antigenic evaluation of hyaluronidase antigenic fragments recombinant protein of streptococcus pyogenes . Afr J Biotechnol 2012;11:2376-2380.
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6. Mirjamali N, Soufian, S, Molaee N, Sadoogh Abbasian S, Abtahi H. Cloning and expression of the enzymatic region of Streptococcal hyaluronidase. Iran J Basic Med Sci 2014; 17:667–672.
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7. Abtahi H, Salmanian AH, Rafati S, Nejad GB, Hassan ZM. High level expression of recombinant ribosomal protein (L7/L12) from Brucella abortus and its reaction with infected human sera. Iran Biomed J 2004; 8:13–18.
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8. Sadoogh Abbasian S, Ghaznavi-Rad E, Akbari N, Zolfaghari M R, pakzad I and Abtahi H. Overexpression and enzymatic assessment of antigenic fragments of hyaluronidase recombinant protein from streptococcus pyogenes. Jundishapur J Microbiol 2015; 8: e13653.
8
9. Fahimirad S, Razavi SH, Abtahi H, Alizadeh H, Ghorbanpour M. Recombinant production and antimicrobial assessment of beta casein-IbAMP 4 as a novel antimicrobial polymeric protein and its synergistic effects with thymol. Int J Pept Res Ther 2018;24:213-222.
9
10. Sadoogh Abbasian S, Soufian S, Ghaznavi-Rad E, Abtahi H. High level activity of recombinant lysostaphin after computer simulation and additive-based refolding. Int J Pept Res Ther 2019; 25:1241–1249.
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11. Fahimirad S, Abtahi H, Razavi SH, Alizadeh H, Ghorbanpour M. Production of recombinant antimicrobial polymeric protein beta casein-E 50-52 and its antimicrobial synergistic effects assessment with yhymol. Molecules 2017;22:822-836.
11
12. Elshikh M, Ahmed S, Funston S, Dunlop P, McGaw M, Marchant R, et al. Resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants. Biotechnol Lett 2016;38:1015-1019.
12
13. Fahimirad S, Ghaznavi-Rad E, Abtahi H, Sarlak N. Antimicrobial activity, stability and wound healing performances of chitosan nanoparticles loaded recombinant LL37 antimicrobial peptide. Int J Pept Res Ther 2021;27:2505-2515.
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14. Rudilla H, Fusté E, Cajal Y, Rabanal F, Vinuesa T, Viñas M. Synergistic antipseudomonal effects of synthetic peptide AMP38 and carbapenems. Molecules 2016;21:1223-1234.
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15. Zengin H, Baysal AH. Antibacterial and antioxidant activity of essential oil terpenes against pathogenicand spoilage-forming bacteria and cell structure-activity relationships evaluated by SEM
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microscopy . Molecules 2014;19:17773-17798.
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17. Cirioni O, Giacometti A, Ghiselli R, Bergnach C, Orlando F, Silvestri C, et al. LL-37 protects rats against lethal sepsis caused by Gram-negative bacteria. Antimicrob Agents Chemother 2006;50:1672-1679.
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21. Fan X, Schäfer H, Reichling J, Wink M. Bactericidal properties of the antimicrobial peptide Ib-AMP4 from Impatiens balsamina produced as a recombinant fusion-protein in Escherichia coli.Biotechnol J 2013;8:1213-1220.
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22. Satei P, Ghaznavi-Rad E, Fahimirad S, Abtahi H. Recombinant production of Trx-Ib-AMP4 and Trx-E50-52 antimicrobial peptides and antimicrobial synergistic assessment on the treatment of methicillin-resistant Staphylococcus aureus under in vitro and in vivo situations. Protein Expr Purif 2021;188:105949.
23
23. Fan X, Reichling J, Wink M. Antibacterial activity of the recombinant antimicrobial peptide Ib-AMP4 from Impatiens balsamina and its synergy with other antimicrobial agents against drug resistant bacteria. Pharmazie 2013; 68:628-630.
24
ORIGINAL_ARTICLE
Oral colonization of Acinetobacter baumannii in intensive care units: Risk factors, incidence, molecular epidemiology, association with the occur of pneumonia and sepsis, and infection control measures
Objective(s): Oral colonization of Acinetobacter baumannii can lead to infections such as pneumonia and sepsis. We aimed to evaluate oral colonization of hospitalized patients in ICUs and to examine risk factors for oral colonization, molecular epidemiology, and incidence of pneumonia and sepsis. Materials and Methods: The study began in February 2021. Oral cultures were taken. The microorganisms were identified by a Maldi-tof MS mass spectrometry device. Colistin resistance genes were investigated by polymerase chain reaction. Clonal relationships were determined by pulsed-field gel electrophoresis. Results: A. baumannii was found in 21 of 96 patients’ oral cultures. Pneumonia and sepsis due to A. baumannii were detected in 14 and 5 patients, respectively. The mean growth time of A. baumannii from oral cultures was 11.8 days, and the meantime for the occurrence of pneumonia after oral growth was 5.2 days. We determined a plasmid mediated mcr-2 colistin resistance gene in a colistin susceptible A. baumannii strain. It is the first report of the plasmid mediated mcr-2 colistin resistance gene in our country. In total, fourteen different A. baumannii genotypes were determined in PFGE. It was determined that the effects of antibiotic use, oral motor dysfunction, mechanical ventilation, intubation, orogastric tube use, and total parenteral nutrition intake on oral colonization were statistically significant.Conclusion: Oral colonization of A. baumannii is a significant concern in ICUs. We believe that it is important to take oral cultures and follow the risk factors and take infection control measures to prevent oral colonization of resistant isolates in ICUs.
https://ijbms.mums.ac.ir/article_19580_837de1691ac52b5882f0d375cd37f80b.pdf
2022-02-01
239
244
10.22038/ijbms.2022.59713.13243
Acinetobacter baumannii
Infection control measures
Intensive Care Units
mcr-2
Oral colonization
Yucel
Duman
yucel.duman@inonu.edu.tr
1
Medical Microbiology Department. Inonu University Medical Faculty, Malatya, Turkey
LEAD_AUTHOR
Yasemin
Ersoy
yasemin.ersoy@inonu.edu.tr
2
Infection Disease Department. Inonu University Medical Faculty, Malatya, Turkey
AUTHOR
Elif
Tanriverdi
seren.tanriverdi@inonu.edu.tr
3
Medical Microbiology Department. Inonu University Medical Faculty, Malatya, Turkey
AUTHOR
Baris
Otlu
botlu@yahoo.com
4
Medical Microbiology Department. Inonu University Medical Faculty, Malatya, Turkey
AUTHOR
Sibel
Altunisik Toplu
sibel.toplu@inonu.edu.tr
5
Infection Disease Department. Inonu University Medical Faculty, Malatya, Turkey
AUTHOR
Harika
Gozukara Bag
harika.gozukara@inonu.edu.tr
6
Biostatistics and Medical Informatics Department. Inonu University Medical Faculty, Malatya, Turkey
AUTHOR
Sait
Tekerekoglu
sait.tekerekoglu@inonu.edu.tr
7
Medical Microbiology Department. Inonu University Medical Faculty, Malatya, Turkey
AUTHOR
Nazire
Bulam
nbulam@hotmail.com
8
Infection Control Committee, Turgut Ozal Medical Center, Malatya, Turkey
AUTHOR
Elif
Kaplan Canturk
elif.canturk@inonu.edu.tr
9
Infection Control Committee, Turgut Ozal Medical Center, Malatya, Turkey
AUTHOR
Nalan
Parmaksiz
nalanparmaksiz@hotmail.com
10
Infection Control Committee, Turgut Ozal Medical Center, Malatya, Turkey
AUTHOR
1. Alp E, Coruh A, Gunay GK, Yontar Y, Doganay M. Risk factors for nosocomial infection and mortality in burn patients: 10 years of experience at a university hospital. J Burn Care Res 2012; 33:379e85.
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2. Gao L, Xu T, Huang G, Jiang S, GuChen YF. Oral microbiomes: more and more importance in oral cavity and whole body. Protein Cell 2018; 9:488–500.
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3. Zaatout N. Presence of non-oral bacteria in the oral cavity. Arch Microbiol 2021; 31:1–14.
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4. Tacconelli E, Magrini N. Global priority list of antibiotic resistant bacteria to guide research, discovery and development of new antibiotics. World Health Organization 2017.
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5. Duman Y, Tekerekoglu MS. Colistin MICs and resistance genes of Acinetobacter baumannii isolated in intensive care units. Turk J Intensive Care 2021; 19:71-75.
5
6. Duman Y, Ersoy Y, Gursoy NC, Altunisik Toplu S, Otlu B. A silent outbreak due to Klebsiella pneumoniae that co-produced NDM-1 and OXA-48 carbapenemases, and infection control measures. Iran J Basic Med Sci 2020; 23:46-50.
6
7. Liu YY, Wang Y, Walsh TR, Yi LX, Zhang R, Spencer J, et al. Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study. Lancet Infect Dis 2016; 16: 161-168.
7
8. Xavier BB, Lammens C, Ruhal R, Kumar-Singh S, Butaye P, Goossens H, et al. Identification of a novel plasmid-mediated colistin-resistance gene, mcr-2, in Escherichia coli, Belgium, June 2016. Euro Surveill 2016; 21: 30280.
8
9. Nasr P. Genetics, epidemiology, and clinical manifestations of multidrug-resistant Acinetobacter baumannii. J Hosp Infect 2020; 104:4-11.
9
10. Karageorgopoulos DE, Falagas ME. Current control and treatment of multidrug-resistant Acinetobacter baumannii infections. Lancet 2008; 8: 751-762
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11. Duman Y, Kuzucu C, Ersoy Y, Otlu B. The Effect of sodium dichloroisocyanurate dihydrate to prevent the environmental transmission of multidrug-resistant Acinetobacter Baumannii in hospital settings. Fresenius Environmental Bull 2020;29: 7191-7197.
11
12. European Committee on Antimicrobial Susceptibility Testing. Available from: https://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/Breakpoint_tables/v_11.0_Breakpoint_Tables.pdf Last, Accessed Date: 22.05.2021.
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13. Rebelo AR, Bortolaia V, Kjeldgaard JS, Pedersen SK, Leekitcharoenphon P, Hansen IM, et al. Multiplex PCR for detection of plasmid-mediated colistin resistance determinants, mcr-1, mcr-2, mcr-3, mcr-4 and mcr-5 for surveillance purposes. Euro Surveill 2018; 23:17-00672.
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14. Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Persing DH, et al. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 1995; 33:2233–2239.
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15. Thatrimontrichai A, Pannaraj PS, Janjindamai W, Dissaneevate S, Maneenil G, Apisarnthanarak A. Intervention to reduce carbapenem-resistant Acinetobacter baumannii in a neonatal intensive care unit. Infect Control Hosp Epidemiol 2020; 41:710-5.
15
16. Johnstone L, Spence S, Koziol-McClain J. Oral hygiene care in the pediatric intensive care unit: practise recommendations. Pediatr Nurs 2010;36: 85–97.
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17. Nutman A, Temkin E, Lellouche J, Ben David D, Schwartz D, Carmeli Y. Detecting carbapenem-resistant Acinetobacter baumannii (CRAB) carriage: Which body site should be cultured? Infect Control Hosp Epidemiol 2020; 41:965-967.
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18. Da Collina GA, Tempestini-Horliana ACR, da Silva DFT, Longo PL, Makabe MLF, Pavani C. Oral hygiene in intensive care unit patients with photodynamic therapy: Study protocol for randomised controlled trial. Trials 2017;18:385-393.
18
19. Silva E, Junior LD, Da Silveira FH, Moreno R, Vincent JL. Prevalence and outcomes of infections in Brazilian ICUs: a subanalysis of EPIC II study. Rev Bras Ter Intensiva 2012; 24:143–150.
19
20. Garcia R, Jendresky L, Colbert L, Bailey A, Zaman M, Majumder M. Reducing ventilator-associated pneumonia through advanced oral-dental care: a 48- month study. Am J Crit Care 2009; 18:523–534.
20
21. Azim A, Dwivedi M, Rao PB, Baronia AK, Singh RK, Prasad KN, et al. Epidemiology of bacterial colonization at intensive care unit admission with emphasis on extended-spectrum beta-lactamase- and metallo-beta-lactamase-producing Gram-negative bacteria--an Indian experience. J Med Microbiol 2010; 59:955-960.
21
22. Chaari A, Mnif B, Bahloul M, Mahjoubi F, Chtara K, Turki O, et al. Acinetobacter baumannii ventilator-associated pneumonia: epidemiology, clinical characteristics, and prognosis factors. Int J Infect Dis 2013;17: e1225-1228.
22
23. Sunenshine RH, Wright MO, Maragakis LL, Harris AD, Song X, Hebden J, et al. Multidrug-resistant Acinetobacter infection mortality rate and length of hospitalization. Emerg Infect Dis 2007; 13:97–103.
23
24. Kwon KT, Oh WS, Song JH, Chang HH, Jung SI, Kim SW, et al. Impact of imipenem resistance on mortality in patients with Acinetobacter bacteraemia. J Antimicrob Chemother 2007; 59:525–530.
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25. Central Asian and Eastern European Surveillance of Antimicrobial Resistance, Annual report 2018. World Health Organization, Denmark: 2019.
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26. Central Asian and Eastern European Surveillance of Antimicrobial Resistance, Annual report 2017. World Health Organization, Denmark: 2018.
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27. Umezawa K, Asai S, Ohshima T, Iwashita H, Ohashi M, Sasaki M, et al. Outbreak of drug-resistant Acinetobacter baumannii ST219 caused by oral care using tap water from contaminated hand hygiene sinks as a reservoir. Am J Infect Control 2015; 43:1249-1251.
27
ORIGINAL_ARTICLE
Aerobic training improves blood-brain barrier and neuronal apoptosis in experimental autoimmune encephalomyelitis
Objective(s): Blood-brain barrier (BBB) permeability is central in multiple sclerosis (MS) pathophysiology, and exercise may improve BBB integrity. The current study investigated the prophylactic and/ or therapeutic role of aerobic exercise (EX) training on BBB integrity in experimental autoimmune encephalomyelitis (EAE). Materials and Methods: Forty female Lewis rats were randomly divided into four groups. The experimental groups included: no-EAE induction+ no-exercise (no-EAE+ no-EX), no-EAE induction+ exercise (no-EAE+EX), EAE induction+ no-exercise (EAE+ no-EX), and EAE induction+ exercise (EAE+EX). The no-EAE+EX and EAE+EX groups performed six weeks of progressive aerobic exercise training. GFAP, angiopoietin 1 (Ang-1) expression, tight-junction (TJ) proteins of claudin-5 and occludin were measured as components of BBB integrity and the rate of neuronal apoptosis was evaluated in hippocampi. Results: A significant increase in GFAP and Ang-1 expression (P<0.001) and conversely a down-regulation in TJ proteins (P<0.05) was found in the brains of the no-EAE+EX group compared with the no-EAE+ no-EX group. The expression of GFAP and Ang-1 proteins significantly increased in the hippocampi of the EAE+ no-EX group (P<0.001), whereas aerobic training (in the EAE+EX group) meaningfully reversed such increases (P<0.001). Besides, down-regulated TJ proteins and increased neuronal apoptosis induced by EAE induction (EAE+ no-EX group) were restored and reduced, respectively, by aerobic training in the CNS of the EAE+EX group (P<0.001). Conclusion: The provision of a six-week treadmill aerobic training buffered the detrimental effects of EAE on BBB integrity and consequently neuronal apoptosis.
https://ijbms.mums.ac.ir/article_19659_3256d319ee88474e14318781a63eaff3.pdf
2022-02-01
245
253
10.22038/ijbms.2022.61671.13645
Aerobic training
Astrogliosis
Brain barrier dysfunction
Claudin-5
EAE model
Occludin
Omid
Razi
omid.razi.physio@gmail.com
1
Department of Exercise Physiology, Faculty of Physical Education and Sports Science, Razi University, Kermanshah, Iran
AUTHOR
Abdolhossein
Parnow
parnowabdolhossein@gmail.com
2
Department of Exercise Physiology, Faculty of Physical Education and Sports Science, Razi University, Kermanshah, Iran
LEAD_AUTHOR
Iraj
Rashidi
iraj570@yahoo.com
3
Department of Anatomical Sciences, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
AUTHOR
Nafiseh
Pakravan
n.pakravan@abzums.ac.ir
4
Department of Immunology, School of Medicine, Alborz University of Medical Science, Karaj, Iran
AUTHOR
Seyed Ershad
Nedaei
ershad_nedaei@yahoo.com
5
Department of Physiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
AUTHOR
Robert
Motl
robmotl@uab.edu
6
Department of Kinesiology and Nutrition, College of Applied Health Sciences, University of Illinois Chicago, United States of America
AUTHOR
1. Guo LY, Lozinski B, Yong VW. Exercise in multiple sclerosis and its models: focus on the central nervous system outcomes. J Neurosci Res 2020; 98:509-523.
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2. Ghaffarinia A, Jalili C, Riazi-Rad F, Mostafaie A, Parvaneh S, Pakravan N. Anti-inflammatory effect of chymotrypsin to autoimmune response against CNS is dose-dependent. Cell Immunol 2014; 292:102-108.
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3. Troletti CD, de Goede P, Kamermans A, de Vries HE. Molecular alterations of the blood–brain barrier under inflammatory conditions: the role of endothelial to mesenchymal transition. Biochim Biophys Acta 2016; 1862:452-460.
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4. Brosnan C. Characteristics of a reactive astrogliosis in multiple sclerosis. Revista Espanola De Esclerosis Multiple 2013; 28:10-18.
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5. Małkiewicz MA, Szarmach A, Sabisz A, Cubała WJ, Szurowska E, Winklewski PJ. Blood-brain barrier permeability and physical exercise. J Neuroinflammation 2019; 16:1-16.
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6. Abbott NJ, Rönnbäck L, Hansson E. Astrocyte–endothelial interactions at the blood–brain barrier. Nat Rev Neurosci 2006; 7:41-53.
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7. Liddelow SA, Barres BA. Reactive astrocytes: production, function, and therapeutic potential. Immunity 2017; 46:957-967.
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ORIGINAL_ARTICLE
The effects of combined resveratrol and high intensity interval training on the hippocampus in aged male rats: An investigation into some signaling pathways related to mitochondria
Objective(s): High-intensity interval training (HIIT) is a shape of interval training that provides ameliorated athletic capacity and has a good effect on health. Resveratrol is a natural polyphenol abundant in grapes and red wine and has been demonstrated to apply various useful health impacts on the body. This research aimed to evaluate the interactive effects of swimming HIIT and resveratrol consumption on SIRTs 3 & 4, NAD+/NADH, AMPK and SOD2 expression in aged rats.Materials and Methods: In total, forty-five old male albino rats (Wistar) with the age of twenty months were allocated into 5 groups randomly. Control group (Ctrl), Swimming HIIT group (Ex: Exercise), Swimming HIIT with Resveratrol consumption group (R+Ex), Resveratrol consumption group (R) and solvent of resveratrol consumption group (vehicle). R+Ex group accomplished the exercise and consumed resveratrol (10 mg/kg/day, gavage) for 6 weeks.Results: HIIT & resveratrol significantly increased NAD+/NADH, SOD 2 and AMPK in the aged rats. HIIT increased SIRT3, but resveratrol reduced it. As for SIRT4, HIIT decreased it, while resveratrol positively affected it.Conclusion: Resveratrol and HIIT, especially their combination, have anti-oxidant and anti-aging effects on the hippocampus of old rats.
https://ijbms.mums.ac.ir/article_19660_0188c463b050d1c8a2da6562b2872603.pdf
2022-02-01
254
262
10.22038/ijbms.2022.57780.12853
Aging
Hippocampus
HIIT exercise
Oxidative stress
Rat
Resveratrol
Sirtuins
Maryam
Amirazodi
mamirazodii@yahoo.com
1
Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
AUTHOR
Amin
Mehrabi
mehrabi64@ut.ac.ir
2
Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
LEAD_AUTHOR
Mohammad Amin
Rajizadeh
aminrajizadeh@yahoo.com
3
Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
AUTHOR
Mohammad Abbas
Bejeshk
m.bejeshk@yahoo.com
4
Department of Physiology and Pharmacology, Kerman University of Medical Sciences, Kerman, Iran
AUTHOR
Khadijeh
Esmaeilpour
khadijeh.esmaeilpour@yahoo.com
5
Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
AUTHOR
Farhad
Daryanoosh
daryanoosh@shirazu.ac.ir
6
Department of Exercise Physiology, Shiraz University, Shiraz, Iran
AUTHOR
Abbasali
Gaeini
aagaeini@ut.ac.ir
7
Department of Exercise Physiology, University of Tehran, Tehran, Iran
AUTHOR
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ORIGINAL_ARTICLE
Lavender protects H9c2 cardiomyocytes against oxygen-glucose deprivation (OGD)-induced injury via targeting the JAK2/STAT3 pathway
Objective(s): This study was conducted to examine the therapeutic effects of lavender oil (LO) against oxygen-glucose deprivation (OGD)-induced injury in vitro model.Materials and Methods: In this study, the OGD model was induced in the H9C2 cell line, and then the cells were treated with LO (10, 100, 1000, and 10000 μg/ml). The anti-inflammatory activity of LO (Jak2/Stat3) was evaluated by immunocytochemical assay. Likewise, the p-ERK/ERK level was measured by western blotting.Results: Compared with only the OGD-induced injury model, cell survival increased after treatment with LO. Our results showed that 100 μg/ml of LO significantly decreased the expression of Jak2/Stat3 and the apoptotic activity 72 hr after reperfusion compared with the control group. Likewise, significant increases were observed in p-ERK/ERK in LO-treated groups. Conclusion: Collectively, these findings confirm that LO can be a good candidate to reduce OGD-induced injury in the H9C2 cell line through targeting Jak2/Stat3 and ERK pathways.
https://ijbms.mums.ac.ir/article_19589_c2fea7e8ca66e5c324d67ab83a149042.pdf
2022-02-01
263
267
10.22038/ijbms.2022.54751.12280
H9C2 cells
Inflammation
Ischemia/Reperfusion injury
Jak2 / Stat3
Lavender oil
p-ERK / ERK
Shaghayegh
Askarian-Amiri
askarianamiri.s@gmail.com
1
Institut de Biologie Structurale (IBS), Univ. Grenoble Alpes, CEA, CNRS, 71 Avenue des Martyrs, 38000 Grenoble, France
AUTHOR
Hoda
Fotovat eskandari
hoda_fotovat@yahoo.com
2
Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran
AUTHOR
Fatemeh
Ramezani
ramezani.f@iums.ac.ir
3
Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran
AUTHOR
Gelare
Vahabzadeh
nasimsaha2022@gmail.com
4
Department of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
AUTHOR
Nahid
Aboutaleb
dr_nabo40@yahoo.com
5
Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran
LEAD_AUTHOR
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30
ORIGINAL_ARTICLE
Erratum: Breviscapine prevents downregulation of renal water and sodium transport proteins in response to unilateral ureteral obstruction
https://ijbms.mums.ac.ir/article_19661_782a53beb2c34c5b6169e160f4e0819e.pdf
2022-02-01
10.22038/ijbms.2022.19661