Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
22
6
2019
06
01
Signaling pathways involved in chronic myeloid leukemia pathogenesis: the importance of targeting Musashi2-Numb signaling to eradicate leukemia stem cells
581
589
EN
Foruzan
Moradi
0000-0002-4431-8622
Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
foruzanmoradi69@yahoo.com
Sadegh
Babashah
0000-0001-7066-0918
Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
sadegh.babashah@gmail.com
Majid
Sadeghizadeh
0000-0002-2497-3152
Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
sadeghma@modares.ac.ir
Arsalan
Jalili
Hematopoietic Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
jalili.arsalan@yahoo.com
Abbas
Hajifathali
Hematopoietic Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
a.hajifathali@sbmu.ac.ir
Elham
Roshandel
Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
elham.roshandel@gmail.com
10.22038/ijbms.2019.31879.7666
<em><strong>Objective(s):</strong></em> Chronic myeloid leukemia (CML) is a myeloid clonal proliferation disease defining by the presence of the Philadelphia chromosome that shows the movement of BCR-ABL1. In this study, the critical role of the Musashi2-Numb axis in determining cell fate and relationship of the axis to important signaling pathways such as Hedgehog and Notch that are essential for self-renewal pathways in CML stem cells will be reviewed meticulously.<br /><em><strong>Materials and Methods:</strong></em> In this review, a PubMed search using the keywords of Leukemia, signaling pathways, Musashi2-Numb was performed, and then we summarized different research works.<br /><em><strong>Results:</strong></em> Although tyrosine kinase inhibitors such as Imatinib significantly kill and remove the cell with BCR-ABL1 translocation, they are unable to target BCR-ABL1 leukemia stem cells. The main problem is stem cells resistance to Imatinib therapy. Therefore, the identification and control of downstream molecules/ signaling route of the BCR-ABL1 that are involved in the survival and self-renewal of leukemia stem cells can be an effective treatment strategy to eliminate leukemia stem cells, which supposed to be cured by Musashi2-Numb signaling pathway.<br /><em><strong>Conclusion:</strong></em> The control of molecules /pathways downstream of the BCR-ABL1 and targeting Musashi2-Numb can be an effective therapeutic strategy for treatment of chronic leukemia stem cells. While Musashi2 is a poor prognostic marker in leukemia, in treatment and strategy, it has significant diagnostic value.
BCR-ABL1,Chronic myeloid leukemia,Cancer stem cells,Signaling pathways,Self-renewal,Targeted therapy
https://ijbms.mums.ac.ir/article_12723.html
https://ijbms.mums.ac.ir/article_12723_d9b444b5e7550b9883ddcd7802ad293e.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
22
6
2019
06
01
The role of Iranian medicinal plants in experimental surgical skin wound healing: An integrative review
590
600
EN
Amin
Derakhshanfar
0000-0001-6154-9468
Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
cbrc@sums.ac.ir
Javad
Moayedi
0000-0001-6154-9468
Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
javad.moayedi88@gmail.com
Ghazal
Derakhshanfar
Center of Comparative and Experimental Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
ghazal.derakhshanfar@gmail.com
Ali
Poostforoosh Fard
Vice Chancellery for Research Affairs, Shiraz University of Medical Sciences, Shiraz, Iran
alipo58@gmail.com
10.22038/ijbms.2019.32963.7873
<em><strong>Objective(s):</strong></em> Wounds are physical injuries that cause a disturbance in the normal skin anatomy and function. Also, it has a severe impact on the cost of health care. Wound healing in human and mammalian species is similar and contains a complex and dynamic process consisting of four phases for restoring skin cellular structures and tissue layers. Today, therapeutic approaches using herbal medicine have been considered. Although the benefits of herbal medicine are vast, some medicinal plants have been shown to have wound healing effects in different experimental studies. Therefore, the current review highlights information about the potency of herbal medicine in the experimental surgical skin wound healing.<br /><em><strong>Materials and Methods:</strong></em> Electronic database such as PubMed, Google Scholar, Scopus, and Medscape were searched for Iranian medicinal plants with healing activity in experimental surgical skin wounds. In this area, some of the most important papers were included.<br /><em><strong>Results:</strong></em> There are numerous Iranian medicinal plants with skin wound healing activity, but clinical application and manufacturing are very low in comparison to the research volume.<br /><em><strong>Conclusion:</strong></em> In normal instances, the human/animal body usually can repair tissue damage precisely and completely; therefore, the utilization of herbs is limited to special conditions or in order to accelerate the healing process.
Experimental,Iranian medicinal plants,Skin,Surgical,Wound healing
https://ijbms.mums.ac.ir/article_12771.html
https://ijbms.mums.ac.ir/article_12771_b58a584b8d3331a8bc184a2c827d6fcf.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
22
6
2019
06
01
The effects of crocin on spatial memory impairment induced by hyoscine: Role of NMDA, AMPA, ERK, and CaMKII proteins in rat hippocampus
601
609
EN
Maliheh
Adabizadeh
0000-0001-9044-776X
Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
adabizadehm2@mums.ac.ir
Soghra
Mehri
0000-0002-9697-2343
Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
mehris@mums.ac.ir
Mahshid
Rajabpour
Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
mahshidr@mums.ac.ir
Khalil
Abnous
0000-0001-6314-0164
Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
abnouskh@mums.ac.ir
Marzieh
Rashedinia
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Shiraz University of Medical Sciences Shiraz Iran
rashediniam881@mums.ac.ir
Hossein
Hosseinzadeh
0000-0002-3483-851X
Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
hosseinzadehh@mums.ac.ir
10.22038/ijbms.2019.30138.7266
<em><strong>Objective(s):</strong></em> Crocus sativus L. and its active constituent, crocin, have neuroprotective effects. The effects of crocin on memory impairment have been mentioned in studies but the signaling pathways have not been evaluated. Therefore, the aim of this study was to evaluate the effects of crocin on the hyoscine-induced memory impairment in rat. Additionally, the level of NMDA (N-methyl-D-aspartate receptors), AMPA (α-amino-3-hydroxy-5-methyl-4-isoxazole-propionicd acid), ERK (extracellular signal-regulated kinases), CaMKII (calcium (Ca2+)/calmodulin (CaM)-dependent kinaseII) mRNA and proteins were determined in rat hippocampus. <br /><em><strong>Materials and Methods:</strong></em> Crocin (10, 20, and 40 mg/kg), hyoscine (1.5 mg/kg), normal saline and rivastigmine were administered intraperitoneally to male Wistar rats for 5 days. The effects on memory improvement were studied using Morris water maze (MWM) test. Then, the protein levels of NMDA, AMPA, ERK, pERK, CaMKII and p.CaMKII in hippocampus were analized using the Western blot test. Furthermore, the mRNA levels of NMDA, AMPA, ERK and pCaMKII genes were evaluated using real-time quantitative reverse transcription-polymerase chain reaction (qRT- PCR) method. <br /><em><strong>Results:</strong></em> Aadminestration of crocin (20 mg/kg) and rivastigmine significantly improved learning and memory impairment induced by hyoscine. Also, administration of hyoscine reduced protein level of pERK, while treatment with crocin (20 mg/kg) recovered the protein level. No changes were observed in the protein levels and mRNA gene expression of NMDA, AMPA, ERK, CaMKII and pCaMKII following adminestration of hyoscine or crocin. <br /><em><strong>Conclusion:</strong></em> Adminestration of crocin improved memory and learning. The effect of crocin in this model can be mediated by alteration in pERK protein level in rat hippocampus.
Crocin,Saffron,Memory,Erk,CaMKII,NMDA,AMPA
https://ijbms.mums.ac.ir/article_12562.html
https://ijbms.mums.ac.ir/article_12562_230ff1945b345b5941c00abdb0e76ca9.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
22
6
2019
06
01
Human leukocyte antigen (HLA)-Cw0303, HLA-Cw04, and HLA-Cw07 polymorphisms are associated with susceptibility of rheumatoid arthritis in Chinese Han patients from Southern China
610
616
EN
Chuankun
Yang
0000-0001-6986-2483
Department of Immunology Laboratory, Zhongda Hospital, School of medicine, Southeast University, Nanjing, Jiangsu, 210009, China
yangchuankun1985@126.com
Chunling
Wang
Department of Immunology Laboratory, Zhongda Hospital, School of medicine, Southeast University, Nanjing, Jiangsu, 210009, China
498650591@qq.com
Yan
Shi
Department of Immunology Laboratory, Zhongda Hospital, School of medicine, Southeast University, Nanjing, Jiangsu, 210009, China
249519007@qq.com
Li
Li
0000-0001-7435-8580
Department of Immunology Laboratory, Zhongda Hospital, School of medicine, Southeast University, Nanjing, Jiangsu, 210009, China
lilee_immuno@sina.cn
10.22038/ijbms.2019.33557.8007
<em><strong>Objective(s):</strong></em> This study aimed to investigate the association between human leukocyte antigen Cw (HLA-Cw) polymorphisms and rheumatoid arthritis (RA) in Chinese Han patients in the Jiangsu area (Southern China).<br /><em><strong>Materials and Methods:</strong></em> Polymerase chain reaction-sequence specific primers were used to detect HLA-Cw01–08 of 201 RA patients and 211 healthy individuals from Zhongda Hospital (China). The allele frequency distribution of HLA-Cw and genotypic differences between the two groups were analyzed.<br /><em><strong>Results:</strong> </em>The frequency of HLA-Cw0303 in patients with RA was significantly higher than that in controls, while the frequency of HLA-Cw04 was lower than that in controls (P<0.05). The gene frequency of HLA-Cw07 in anti-cyclic citrullinated peptide (anti-CCP)-negative patients was higher than that in controls (P=0.044). The frequency of HLA-Cw04 was decreased in the short duration subgroup and increased in the long duration subgroup (P<0.05). Compared to controls, the frequency of HLA-Cw0303 in patients with RA and morning stiffness was increased (P=0.004), while the frequency of HLA-Cw04 was decreased ( 0.005).<br /><em><strong>Conclusion:</strong></em> These results suggest that HLA-Cw0303 is a susceptibility gene for RA in Chinese Han patients in the Jiangsu area of southern China. The HLA-Cw04 gene may be a protective factor against RA, while HLA-Cw07 might play a protective role in the production of anti-CCP in the long-term course in patients with RA.
Anti-citrullinated protein antibodies,HLA antigens,Gene frequency,Polymorphism,Single nucleotide,Arthritis rheumatoid
https://ijbms.mums.ac.ir/article_12583.html
https://ijbms.mums.ac.ir/article_12583_15c1fb0b25122fbb5f1435b5914dc471.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
22
6
2019
06
01
Obestatin inhibits apoptosis and astrogliosis of hippocampal neurons following global cerebral ischemia reperfusion via antioxidant and anti-inflammatory mechanisms
617
622
EN
Elahe
Mirarab
0000-0002-3380-0042
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
elahem@gmail.cm
Vida
Hojati
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
vidah@yahoo.com
Gholamhassan
Vaezi
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
gh.vaezi@yahoo.com
Abdolhossein
Shiravi
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
shiraviab@yahoo.com
Mehdi
Khaksari
0000-0002-2240-1521
School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
khaksari417@yahoo.com
10.22038/ijbms.2019.34118.8110
<strong><em>Objective(s):</em></strong> Obestatin is a newly discovered peptide with antioxidant activities in different animal models. Recent studies have shown that Obestatin inhibits apoptosis following cardiac ischemia/reperfusion injury. Brain ischemia/reperfusion induces irreversible damage especially in the hippocampus area. This study aimed at examining the protective impact of Obestatin on apoptosis, protein expression and reactive astrogliosis level in hippocampal CA1 region of rat following transient global cerebral ischemia.<br /><em><strong>Materials and Methods:</strong> </em>Forty-eight male Wistar rats were randomly assigned into 4 groups (sham, ischemia/reperfusion, ischemia/reperfusion+ Obestatin 1, and 5 µg/kg, n=12). Ischemia induced occlusion of both common carotid arteries for 20 min. Obestatin 1 and 5 µg/kg were injected intraperitoneally at the beginning of reperfusion period and 24 and 48 hr after reperfusion. Assessment of the antioxidant enzymes and tumor necrosis factor alpha (TNF-α) was performed by ELISA method. Caspase-3 and glial fibrillary acidic protein (GFAP) proteins expression levels were evaluated by immunohistochemical staining 7 days after ischemia.<br /><em><strong>Results:</strong></em> Based on the result of the current study, lower superoxide dismutase (SOD) and glutathione (GSH) (P<0.05) and higher malondialdehyde (MDA) and TNF-α levels were observed in the ischemia group than those of the sham group (P<0.01). Obestatin treatment could increase both SOD and GSH (P<0.05) and reduce MDA and TNF-α (P<0.05) versus the ischemia group. Moreover, obestatin could significantly decrease caspase-3 and GFAP positive cells in the CA1 region of hippocampus (P<0.01).<br /> <em><strong>Conclusion:</strong></em> Obestatin exerts protective effects against ischemia injury by inhibition of astrocytes activation and decreases neuronal cell apoptosis via its antioxidant properties.
Apoptosis,Astrogliosis,Brain ischemia,Hippocampus,Obestatin
https://ijbms.mums.ac.ir/article_12761.html
https://ijbms.mums.ac.ir/article_12761_92a4a9f133e2473f90bf69a966134743.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
22
6
2019
06
01
Expression of the receptor of advanced glycation end-products (RAGE) and membranal location in peripheral blood mononuclear cells (PBMC) in obesity and insulin resistance
623
630
EN
Elizabeth del
Ruelas Cinco
0000-0002-4842-5337
Instituto de Investigación en Enfermedades Crónico-Degenerativas, Departamento de Biología Molecular y Genómica, C.U.C.S, Universidad de Guadalajara, Guadalajara, Jalisco, México
ruelas5_eli@hotmail.com
Bertha
Ruíz Madrigal
Laboratorio de Investigación en Microbiología, Departamento de Microbiología y Patología, C.U.C.S, Universidad de Guadalajara, Guadalajara, Jalisco, México
bertharuiz8@yahoo.com.mx
José Alfredo
Dominguez-Rosales
Instituto de Investigación en Enfermedades Crónico-Degenerativas, Departamento de Biología Molecular y Genómica, C.U.C.S, Universidad de Guadalajara, Guadalajara, Jalisco, México
dominque14@yahoo.com
Montserrat
Maldonado González
Laboratorio de Investigación en Microbiología, Departamento de Microbiología y Patología, C.U.C.S, Universidad de Guadalajara, Guadalajara, Jalisco, México
montserratmaldonado@yahoo.com.mx
Lucía
De la Cruz Color
0000-0002-7948-0005
Instituto de Investigación en Enfermedades Crónico-Degenerativas, Departamento de Biología Molecular y Genómica, C.U.C.S, Universidad de Guadalajara, Guadalajara, Jalisco, México
lucia_color@yahoo.com.mx
Sandra
Margarita Ramírez Meza
Laboratorio de Investigación en Microbiología, Departamento de Microbiología y Patología, C.U.C.S, Universidad de Guadalajara, Guadalajara, Jalisco, México
zandhrita_15@hotmail.com
José
Rodrigo Torres Baranda
Laboratorio de Investigación en Microbiología, Departamento de Microbiología y Patología, C.U.C.S, Universidad de Guadalajara, Guadalajara, Jalisco, México
rodrigo.torresbaranda@gmail.com
Erika
Martínez López
Programa de Doctorado en Ciencias en Biología Molecular en Medicina. Departamento de Biología Molecular y Genómica, C.U.C.S, Universidad de Guadalajara, Guadalajara, Jalisco, México
erikamtz27@yahoo.com.mx
Zamira Helena
Hernández Nazará
0000-0003-2319-8470
Instituto de Investigación en Enfermedades Crónico-Degenerativas, Departamento de Biología Molecular y Genómica, C.U.C.S, Universidad de Guadalajara, Guadalajara, Jalisco, México
zamirahelena@yahoo.com.mx
10.22038/ijbms.2019.34571.8206
<em><strong>Objective(s):</strong></em> The present study aimed to evaluate the receptor of advanced glycation end-products (RAGE), NF-kB, NRF2 gene expression, and RAGE cell distribution in peripheral blood mononuclear cells (PBMC) in subjects with obesity and IR compared with healthy subjects.<br /><em><strong>Materials and Methods:</strong></em> The mRNA expression levels of RAGE, NF-kB, NRF2, and GAPDH were determined in PBMC by qPCR in 20 obese (OB), 17 obese with insulin resistance (OB-IR) subjects, and 20 age and sex-matched healthy subjects (HS). RAGE protein expression and its localization were determined by Western Blot and immunocytochemistry (ICC) analysis, total soluble RAGE (sRAGE) and MCP-1 plasma levels by ELISA. <br /><em><strong>Results:</strong></em> RAGE, NF-kB, and NRF2 genes mRNA expression in PBMCs did not show variation between groups. RAGE protein was lower in OB and OB-IR groups; RAGE was located predominantly on the cell-surface in the OB-IR group compared to the HS group (22% vs 9.5%, P<0.001). OB-IR group showed lower sRAGE plasma levels, and correlated negatively with HOMA-IR, ALT parameters (r= -0.374, r= -0.429, respectively), and positively with NFE2L2 mRNA (r= 0.540) P<em><strong>Conclusion:</strong></em> In this study, OB-IR subjects did not reflect significant differences in gene expression; however, correlations detected between sRAGE, biochemical parameters, and NRF2, besides the predominant RAGE distribution on the cell membrane in PBMC could be evidence of the early phase of the inflammatory cascade and the subsequent damage in specific tissues in subjects with OB-IR.
AGER protein human,insulin resistance,Obesity,Oxidative stress,Receptor for advanced glycation end products
https://ijbms.mums.ac.ir/article_12763.html
https://ijbms.mums.ac.ir/article_12763_55edd33c9cb183a01c9b2cfb8912f745.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
22
6
2019
06
01
Effects of Lactobacillus acidophilus and Bifidobacterium bifidum probiotics on the serum biochemical parameters, and the vitamin D and leptin receptor genes on mice colon cancer
631
636
EN
Peyman
Ranji
0000-0002-8842-1592
Cancer Research Center, Tehran University of Medical Sciences, Tehran, Iran
peyman_ranji@yahoo.com
Shahram
Agah
Colorectal Research Center, Iran University of Medical Sciences, Tehran, Iran
dr.sh.agah@gmail.com
Zahra
Heydari
Cancer Research Center, Tehran University of Medical Sciences, Tehran, Iran
heydarizara@yahoo.com
Mohammad
Rahmati-Yamchi
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
rahmatibio@gmail.com
Ali Mohammad
Alizadeh
0000-0002-9885-7066
Cancer Research Center, Tehran University of Medical Sciences, Tehran, Iran
aalizadeh@sina.tums.ac.ir
10.22038/ijbms.2019.32624.7806
<em><strong>Objective(s):</strong></em> The preclinical reports have shown that specific probiotics like Bifidobacterium bifidum (B. bifidum) and Lactobacillus acidophilus (L. acidophilus) can be applied as the biotherapeutic agents in the inhibition or therapy of colorectal cancer via the modification of gut bacteria. In the previous studies, we have assessed the impact of L. acidophilus and B. bifidum probiotics on gut bacteria concentration and also their chemo-protective impact on mice colon cancer. In the following, we assessed the effects of these probiotics on the gene expression of vitamin D receptor (VDR) and the leptin receptor (LPR) and the serum biochemical parameters on mice colon cancer. <br /><em><strong>Materials and Methods:</strong></em> Thirty-six male BALB/c mice were equally shared into 4 groups; (i) health with routine dietary foods without any treatment, (ii) azoxymethane (AOM)-induced mice colon cancer with common dietary foods, (iii) and (iv) AOM-induced mice colon cancer with oral consumption of L. acidophilus and B. bifidum (1×109 cfu/g) for 5 months, respectively. Then, the serum total cholesterol, triglycerides, low-density lipoprotein cholesterol (LDL), high-density lipoprotein cholesterol (HDL), alanine transaminase, alkaline phosphatase, and albumin and also VDR and LPR genes expression were evaluated. <br /><em><strong>Results:</strong></em> Oral consumption of L. acidophilus and B. bifidum probiotics significantly decreased the triglycerides, alkaline phosphatase, LDL, and also the VDR and LPR gene expression in mice colon cancer (P<0.005).<br /><em><strong>Conclusion:</strong></em> L. acidophilus and B. bifidum probiotics with the modification of the biochemical parameters and the expression of the VDR and LPR genes can play a key role in the protection of mouse colon cancer.
Colon cancer,Leptin receptor,Mice,Probiotic,Vitamin D Receptor
https://ijbms.mums.ac.ir/article_12582.html
https://ijbms.mums.ac.ir/article_12582_e079eecb9e4238dcab3558261d90c7e1.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
22
6
2019
06
01
Effect of troxerutin on apelin-13, apelin receptors (APJ), and ovarian histological changes in the offspring of high-fat diet fed rats
637
642
EN
Keivan
Mehri
0000-0002-9922-4666
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
keivanm20@gmail.com
Seyed Mehdi
Banan Khojasteh
Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Iran
smbanan@tabrizu.ac.ir
Fereshteh
Farajdokht
0000-0001-5349-6028
Neurosciences Research Center (NSRC), Tabriz University of Medical Sciences, Tabriz, Iran
farajdokht@gmail.com
Zohreh
Zavvari Oskuye
0000-0002-1667-1082
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
zohre.zavari@gmail.com
Hadi
Ebrahimi
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
ehadi2@yahoo.com
Rogaye
Diba
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
r1367diba@gmail.com
Parvin
Bayandor
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
bayandorbarzegari@gmail.com
Maryam
Hosseindoost
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
mhh.hosseindoost@gmail.com
Shirin
Babri
0000-0002-0256-3152
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
shirinb46@yahoo.com
10.22038/ijbms.2019.34158.8123
<em><strong>Objective(s):</strong></em> Maternal high-fat diet (HFD) consumption has been linked to metabolic disorders and reproductive dysfunctions in offspring. Troxerutin (TRO) has anti-hyperlipidemic, anti-oxidant, and anti-inflammatory effects. This study examined the effects of TRO on apelin-13, its receptors mRNA and ovarian histological changes in the offspring of HFD fed rats. <br /><em><strong>Materials and Methods:</strong></em> Female Wistar rats were randomly divided into control diet (CD) or HFD groups and received these diets for eight weeks. After mating, dams were assigned into four subgroups: CD, CD + TRO, HFD, and HFD + TRO, and received their respective diets until the end of lactation. Troxerutin (150 mg/kg/day) was gavaged in the CD + TRO and HFD + TRO groups during pregnancy. On the postnatal day (PND) 21 all female offspring were separated and fed CD until PND 90. On PND 90 animals were sacrificed and ovarian tissue samples were collected for further evaluation. <br /><em><strong>Results:</strong></em> Results showed that HFD significantly decreased serum apelin-13 in the female offspring of the HFD dams, which was significantly reversed by TRO. Moreover, real-time polymerase chain reaction (PCR) analysis revealed that TRO treatment significantly decreased the ovarian mRNA expression of the apelin-13 receptor in the troxerutin-received offspring. Furthermore, histological examination revealed that TRO increased the number of atretic follicles in the ovaries of HFD+TRO offspring.<br /><em><strong>Conclusion:</strong></em> Maternal high fat feeding compromises ovarian health including follicular growth and development in the adult offspring and troxerutin treatment improved negative effects of maternal HFD on the apelin-13 level and ovarian development of offspring.
Apelin-13,APJ receptor,Maternal high-fat diet,Ovarian development,Troxerutin
https://ijbms.mums.ac.ir/article_12762.html
https://ijbms.mums.ac.ir/article_12762_5d2c3f6bc77dc2c601ad73ea1f6f375a.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
22
6
2019
06
01
Studying humane endpoints in a rat model of mammary carcinogenesis
643
649
EN
Ana I
Faustino-Rocha
0000-0001-5572-6317
Faculty of Veterinary Medicine, Lusophone University of Humanities and Technologies, Lisbon, Portugal
anafaustino.faustino@sapo.pt
Mário
Ginja
Center for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal
mginja@utd.pt
Rita
Ferreira
Organic Chemistry, Natural Products and Foodstuffs (QOPNA), Mass Spectrometry Center, Department of Chemistry, University of Aveiro, Aveiro, Portugal
ritaferreira@ua.pt
Paula A
Oliveira
Center for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal
pamo@utad.pt
10.22038/ijbms.2019.33331.7957
<em><strong>Objective(s):</strong></em> The present work intended to clearly define the most adequate humane endpoints in an experimental assay of mammary carcinogenesis in rats. <br /><em><strong>Materials and Methods:</strong></em> Animals were observed twice a day; all parameters were registered once a week and the euthanasia endpoints were established in order to monitor the animal welfare/distress during an experimental assay of chemically-induced mammary carcinogenesis in female rats. <br /><em><strong>Results:</strong></em> Fourteen animals developed at least one mammary tumor with a diameter >35 mm. No animals exhibited alterations in the remaining parameters that implied their early sacrifice. Statistically significant changes were not observed in the quantitative parameters like the hematocrit and urine specific gravity among groups, not being valuable for the assessment of the health status of animals included in an assay of mammary carcinogenesis for 18 weeks. The remaining humane endpoints seemed to be helpful to monitor the animals’ health status.<br /><em><strong>Conclusion:</strong></em> The alteration in only one humane endpoint (mammary tumor dimensions) does not imply the animals’ sacrifice; the endpoints should be evaluated in conjunction, in order to define the most adequate time in which the animals should be sacrificed.
Chemically-induced,Humane endpoints,Mammary cancer,N-methyl-N-nitrosourea,Rat,Welfare
https://ijbms.mums.ac.ir/article_12773.html
https://ijbms.mums.ac.ir/article_12773_f3cbc32b2a86e7c13e4bf2745efd4dad.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
22
6
2019
06
01
Pulsed electromagnetic field attenuated PTSD-induced failure of conditioned fear extinction
650
659
EN
Mohammad Ali
Mohammad Alizadeh
School of Biology, Damghan University, Damghan, Semnan, Iran
maz.4747@yahoo.com
Kataneh
Abrari
0000-0002-8056-9218
School of Biology, Damghan University, Damghan, Semnan, Iran
abrari@du.ac.ir
Taghi
Lashkar Blouki
0000-0002-5145-3937
School of Biology, Damghan University, Damghan, Semnan, Iran
lashkar@du.ac.ir
mohammad taghi
ghorbanian
0000-0001-8173-5508
School of Biology, Damghan University, Damghan, Semnan, Iran
ghorbanian@du.ac.ir
Majid
Jadidi
0000-0001-6892-7695
Department of Medical Physics, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran
jadidim@semums.ac.ir
10.22038/ijbms.2019.32576.7797
<em><strong>Objective(s):</strong></em> This study aimed to determine whether exposure to pulsed electromagnetic field (PEMF) can impair behavioral failure as induced by PTSD, and also its possible effects on hippocampal neurogenesis. PEMF was used as a non-invasive therapeutic tool in psychiatry.<br /><em><strong>Materials and Methods:</strong></em> Male rats were divided into Control-Sham exposed, Control-PEMF, PTSD-Sham exposed, and PTSD-PEMF groups. PTSD rats were conducted by the single prolonged stress procedures and then conditioned by the contextual fear conditioning apparatus. Control rats were only conditioned. Experimental rats were submitted to daily PEMF (7 mT, 30 Hz for 16 min/day, 14 days). Sham-exposed groups were submitted to the turned off PEMF apparatus. Fear extinction, sensitized fear and anxiety, cell density in the hippocampus, and proliferation and survival rate of BrdU-labeled cells were evaluated. <br /><em><strong>Results:</strong></em> Freezing of PTSD-PEMF rats was significantly lower than PTSD-Sham exposed. In the PTSD-PEMF, center and total crossing in open field, also the percentage of open arms entry and time in the elevated plus maze, significantly increased as compared with PTSD-Sham exposed (P<0.001). Numbers of CA1, CA3, and DG cells in PTSD-PEMF and Control-Sham exposed groups were significantly more than PTSD-Sham exposed (P<0.001). There were more BrdU-positive cells in the DG of the PTSD-PEMF as compared with the PTSD-Sham exposed. Qualitative observations showed an increased number of surviving BrdU-positive cells in the PTSD-PEMF as compared with PTSD-Sham exposed.<br /><em><strong>Conclusion:</strong></em> Using 14-day PEM attenuates the PTSD-induced failure of conditioned fear extinction and exaggerated sensitized fear, and this might be related to the neuroprotective effects of magnetic fields on the hippocampus.
Classical conditioning,Electromagnetic Fields,Hippocampus,Neurogenesis,Post-Traumatic Stress,disorder
https://ijbms.mums.ac.ir/article_12720.html
https://ijbms.mums.ac.ir/article_12720_3aaf1f52e11e5679796f7ad483ccfadb.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
22
6
2019
06
01
Iron chelation effect of curcumin and baicalein on aplastic anemia mouse model with iron overload
660
668
EN
Dijiong
Wu
0000-0003-2338-9365
Department of Hematology, First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China
wdj850@163.com
Xiaowen
Wen
Department of Internal Medicine, Central Hospital of Jinhua Affiliated to Zhejiang University, Jinhua, Zhejiang, China
xiaowenax@163.com
Linglong
Xu
Department of Hematology, Taizhou Central Hospital, Taizhou, Zhejiang, China
daniongniong@163.com
Wenbin
Liu
Department of Hematology, First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China
liouwenbin6666@163.com
Huijin
Hu
Department of Hematology, First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China
huhuijin12@sina.com
Baodong
Ye
0000-0001-5912-2633
Department of Hematology, First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China
13588453501@163.com
Yuhong
Zhou
Department of Hematology, First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China
zyhblood@163.com
10.22038/ijbms.2019.30840.7440
<em><strong>Objective(s):</strong></em> The current work aimed to assess whether curcumin and baicalein can chelate iron in aplastic anemia (AA) complicated with iron overload, exploring the potential mechanisms.<br /><em><strong>Materials and Methods:</strong></em> A mouse model of AA with iron overload complication was firstly established. Low and high-dose curcumin or baicalein treatment groups were set up, as well as the deferoxamine positive control, normal and model groups (n=8). Hemogram and bone marrow mononuclear cell detection were performed, and TUNEL and immunohistochemical staining were used to evaluate hematopoiesis and apoptosis in the marrow. ELISA, Western blot, and qRT-PCR were employed to assess serum iron (SI), serum ferritin (SF), bone morphogenetic protein 6 (BMP-6), SMAD family member4 (SMAD4) and transferrin receptor 2 (TfR2) amounts. <br /><em><strong>Results:</strong></em> Both curcumin and baicalein improved white blood cell (increase of 0.28-0.64×109/l in high-dose groups) and hemoglobin (increase of around 10 g/l) amounts significantly, which may related to decreased apoptosis (nearly 30%-50% of that in the model group) in the bone marrow, while their effects on platelet recovery were limited and inferior to that of deferoxamine (DFO). Both test compounds up-regulated hepcidin and its regulators (BMP-6, SMAD, and TfR2) at the protein and mRNA levels; high dosage treatment may be beneficial, being better than DFO administration in lessening iron deposition in the bone marrow.<br /><em><strong>Conclusion:</strong></em> Curcumin and baicalein protect hematopoiesis from immune and iron overload-induced apoptosis, exerting iron chelation effects in vivo.
Anemia,Animal,Aplastic,Baicalein,Curcumin,Deferoxamine,Iron overload,Mice,Models
https://ijbms.mums.ac.ir/article_12770.html
https://ijbms.mums.ac.ir/article_12770_1e0d52f05545e5352aca6eac7872d4e9.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
22
6
2019
06
01
Study of the immunogenicity of outer membrane protein A (ompA) gene from Acinetobacter baumannii as DNA vaccine candidate in vivo
669
675
EN
Hossein
Ansari
0000-0002-9928-7261
Department of Genetics, Marvdasht branch, Islamic Azad University, Marvdasht, Iran
hosseinansari62@gmail.com
Maryam
Tahmasebi Birgani
0000-0002-9624-1903
Department of Medical Genetics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
maryam_tahmaseby@yahoo.com
Mahdi
Bijanzadeh
0000-0002-5650-9450
Department of Medical Genetics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
mbijanz@yahoo.com
Abbas
Doosti
0000-0001-8052-5963
Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
abbasdoosti@yahoo.com
Mohammad
Kargar
0000-0002-9531-3645
Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran
microkargar@gmail.com
10.22038/ijbms.2019.30799.7427
<em><strong>Objective(s):</strong></em> Acinetobacter baumannii is one the most dangerous opportunistic pathogens in hospitalized infections. This bacterium is resistant to 90% of commercial antibiotics. Therefore, developing new strategies to cure A. baumannii-infections is urgent. The DNA vaccines new approach in which the immunogen can be directly expressed inside the target cells through cloning of immunogen into an expression vector. The outer membrane protein A(OmpA) is one the critical factors in pathogenicity of A. baumannii which has been repeatedly described as a powerful immunogen to trigger the immune responses. As the pure form of the OmpA is insoluble, vaccine delivery is very hard. <br /><em><strong>Materials and Methods:</strong></em> We previously cloned the ompA gene from A. baumannii into the eukaryotic expression vector pBudCE4.1 and observed that the OmpA protein has been considerably expressed in eukaryotic cell model. In current study, the immunogenic potential of pBudCE4.1-ompA has been evaluated in mice model of experimental. The serum levels of IgM, IgG, IL-2, IL-4, IL-12 and INF-γ were measured by enzyme-linked immunosorbent assay (ELISA) after immunization with ompA-vaccine. The protective efficiency of the designed-DNA vaccine was evaluated following intranasal administration of mice with toxic dose of A. baumannii.<br /><em><strong>Results:</strong></em> Obtained data showed the elevated levels of IgM, IgG, IL-2, IL-4, IL-12 and INF-γ in serum following the vaccine administration and mice who immunized with recombinant vector were survived more than control group.<br /><em><strong>Conclusion:</strong></em> These findings indicate ompA-DNA vaccine is potent to trigger humoral and cellular immunity responses although further experiments are needed.
Acinetobacter baumannii,OmpA Outer membrane protein,ompA gene,DNA vaccine,Immunomodulation,In vivo
https://ijbms.mums.ac.ir/article_12760.html
https://ijbms.mums.ac.ir/article_12760_d92356a136a673d0905f1125e2e2bc62.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
22
6
2019
06
01
Mirtazapine may show anti-hyperglycemic effect by decreasing GLUT2 through leptin and galanin expressions in the liver of type 1 diabetic rats
676
682
EN
Ezgi
Bektur
0000-0003-1453-7311
Eskisehir Osmangazi University, Faculty of Medicine, Department of Histology and Embryology, Eskisehir, Turkey
ezgi.bektur@gmail.com
Erhan
ŞAHİN
0000-0003-2152-0542
Eskisehir Osmangazi University, Faculty of Medicine, Department of Histology and Embryology, Eskisehir, Turkey
erhansahinn@gmail.com
Cengiz
Baycu
Okan University, Faculty of Medicine, Department of Histology and Embryology, Istanbul, Turkey
cbaycu@gmail.com
10.22038/ijbms.2019.34529.8190
<em><strong>Objective(s):</strong></em> The aim of this study was to explore the molecular mechanism of mirtazapine with respect to energy metabolism in Streptozotocin-induced diabetic liver of rats by immunohistochemistry and Western blot. <br /><em><strong>Materials and Methods:</strong></em> Twenty-one male Sprague-Dawley rats were assigned into 3 groups including control, type 1 diabetes mellitus (T1DM) group (55 mg/kg Streptozocin, IP) and T1DM+mirtazapine (20 mg/kg,PO) group. At the end of the experiment, blood glucose levels were measured and liver tissues were stained by Periodic acid–Schiff. Moreover, leptin and glucose transporter 2 (GLUT2) proteins were analyzed by western blot and immunohistochemistry; however, galanin were analyzed only by immunohistochemistry.<br /><em><strong>Results:</strong></em> At the end of the study, in diabetes group, blood glucose level, GLUT2 and galanin expressions increased, while leptin expression decreased when compared to control group. Mirtazapine treatment restored the decreased leptin expression, and the increased blood glucose level and galanine expression to the level of the control group. It also decreased the GLUT2 expression even below the control group. <br /><em><strong>Conclusion:</strong></em> We concluded that mirtazapine may show its anti-hyperglycemic effect by decreasing GLUT2 through altering the leptin and galanin expression in the liver of type 1 diabetic rats. Mirtazapine can be used as an antidepressant for T1DM patients and as a drug to reduce blood glucose level in T1DM.
Galanin,GLUT2,Leptin,Liver,Mirtazapine,Type 1 Diabetes Mellitus
https://ijbms.mums.ac.ir/article_12774.html
https://ijbms.mums.ac.ir/article_12774_5841c336a7e3c87495944bbdca75cd1e.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
22
6
2019
06
01
The protective effect of bone marrow-derived mesenchymal stem cells in liver ischemia/reperfusion injury via down-regulation of miR-370
683
689
EN
Mohamad Ali
Zare
0000-0003-0370-2801
Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
zarema@sums.ac.ir
Abdolhossein
Zare
Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
zareab@sums.ac.ir
Negar
Azarpira
0000-0002-5549-0057
Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
negarazarpira@yahoo.com
Sara
Pakbaz
Department of Pathology, University of Toronto, Toronto, Canada
pakbazs@sums.ac.ir
10.22038/ijbms.2019.32670.7812
<em><strong>Objective(s):</strong></em> Liver transplantation is the most important therapy for end-stage liver disease and ischemia reperfusion (I/R) injury is indeed a risk factor for hepatic failure after grafting. The role of miRNAs in I/R is not completely understood. The aim of this study was to investigate the potential protective role of the mesenchymal stem cells (MSCs) and ischemic preconditioning on miR-370 expression and tissue injury in hepatic I/R injury. <br /><em><strong>Materials and Methods:</strong></em> In this study, 24 BALB/c mice were divided into 4 groups, including sham, I/R, I/R mouse that received MSCs (I/R+MSC) and ischemia preconditioning (IPC) The expression levels of hepatic miR-370, Bcl2 and BAX in male BALB/c mice in different groups including hepatic I/R, hepatic I/R received MSCs, and hepatic I/R with IPC were assessed by quantitative real-time PCR. The effect of miR-370 on hepatic I/R was investigated by serum liver enzyme analysis and histological examination. <br /><em><strong>Results:</strong></em> The expression of miR-370 was significantly up-regulated in the mice subjected to hepatic I/R injury as compared with the sham operated mice. Injection of MSCs led to the down-regulation of the serum liver enzymes, expression of miR-370 and BAX, up-regulation of Bcl2 as well as the improvement of hepatic histological damage. IPC led to similar results, but the difference was not significant. <br /><em><strong>Conclusion:</strong></em> Our data suggest that miR-370 affected the Blc2/BAX pathway in hepatic I/R injury, and down- regulation of miR-370 by BM-MSCs efficiently attenuated the liver damage.
Apoptosis,Bcl2,Bax,Ischemia reperfusion injury,Mesenchymal stem cells,microRNA 370
https://ijbms.mums.ac.ir/article_12795.html
https://ijbms.mums.ac.ir/article_12795_9e4dd2373d1010ca3445e66e19ecd35a.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
22
6
2019
06
01
Vitamin D suppresses cellular pathways of diabetes complication in liver
690
694
EN
Hoda
Derakhshanian
0000-0003-3104-6397
Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran
derakhshanian@gmail.com
Mahmoud
Djalali
0000-0002-7357-3964
Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran
mjalali87@yahoo.com
Mohammad Hassan
Javanbakht
Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran
mhjavan2001@yahoo.com
Ehsan
Alvandi
Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran
ehsanalvandi@gmail.com
Mohhamd Reza
Eshraghian
Department of Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
eshraghianmr@yahoo.com
Abbas
Mirshafiey
Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
mirshafiey@tums.ac.ir
Hoda
Nadimi
0000-0002-5945-5005
Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran
hodanadimi@gmail.com
Samane
Jahanabadi
Department of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
sjahanabadi@yahoo.com
Mahnaz
Zarei
Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran
mahnazzareei@yahoo.com
Abolghassem
Djazayery
0000-0001-8666-0090
Department of Community Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran
jazaiers@tums.ac.ir
10.22038/ijbms.2019.36054.8584
<em><strong>Objective(s):</strong> </em>The aim of this study was to investigate the effect of vitamin D on glucose metabolism, as well as the expression of five key genes involved in the development of diabetes complications in liver tissue of diabetic rats. <br /><em><strong>Materials and Methods:</strong></em> Twenty-four male Sprague–Dawley rats were randomly divided into three groups (8 rats in each group). The first group served as control and the other two groups received an intraperitoneal injection of 45 mg/kg streptozotocin to develop diabetes. Groups were treated for four weeks either with placebo or vitamin D (two injections of 20000 IU/kg). Thereafter, serum levels of glucose, insulin and HbA1c were assessed. Liver tissue was examined for the level of advanced glycation end products (AGEs) and the gene expression of AGE cellular receptor (AGER), glyoxalase-1 (GLO-1), aldose reductase (AR), O-linked N-acetylglucosamine transferase (OGT) and glutamine/ fructose-6-phosphate aminotransferase (GFAT). <br /><em><strong>Results:</strong></em> Vitamin D injection resulted in a significant increase in plasma level of 25-hydroxycholecalciferol, which could improve hyperglycemia about 11% compared to placebo-receiving diabetic rats (P=0.005). Insulin level increased as a result of vitamin D treatment compared to control (3.31±0.65 vs. 2.15±0.79; P= 0.01). Serum HbA1c and liver AGE concentrations had a slight but insignificant reduction following vitamin D intake. Moreover, a significant decline was observed in gene expression of AGER and OGT in liver tissue (P=0.04 and P<em><strong>Conclusion:</strong></em> Vitamin D might contribute in ameliorating diabetes complications not only by improving blood glucose and insulin levels, but also by suppressing AGER and OGT gene expression in the liver.
Advanced Glycation End Products,Cholecalciferol,Diabetes Complications,Hexosamine pathway,Vitamin D
https://ijbms.mums.ac.ir/article_12799.html
https://ijbms.mums.ac.ir/article_12799_ee28c6ec62aa21814df38f756072f6a7.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
22
6
2019
06
01
Catechol-o-methyltransferase inhibitor tolcapone improves learning and memory in naïve but not in haloperidol challenged rats
695
702
EN
Anita
Mihaylova
0000-0002-8472-2849
Department of Pharmacology and Drug Toxicology, Faculty of Pharmacy, Medical University Plovdiv, 15A Vassil Aprilov Blvd., Plovdiv 4002, Bulgaria
animihailova@gmail.com
Hristina
Zlatanova
0000-0003-2555-4600
Department of Pharmacology and Clinical Pharmacology, Faculty of Medicine, Medical University Plovdiv, 15A Vassil Aprilov Blvd., Plovdiv 4002, Bulgaria
h.zlatanova@gmail.com
Nina
Doncheva
0000-0001-5978-6237
Department of Pharmacology and Drug Toxicology, Faculty of Pharmacy, Medical University Plovdiv, 15A Vassil Aprilov Blvd., Plovdiv 4002, Bulgaria
ninanina1972@abv.bg
Delian
Delev
Department of Pharmacology and Clinical Pharmacology, Faculty of Medicine, Medical University Plovdiv, 15A Vassil Aprilov Blvd., Plovdiv 4002, Bulgaria
delevg@gmail.com
Ilia
Kostadinov
Department of Pharmacology and Clinical Pharmacology, Faculty of Medicine, Medical University Plovdiv, 15A Vassil Aprilov Blvd., Plovdiv 4002, Bulgaria
ilia_197575@abv.bg
10.22038/ijbms.2019.33025.7890
<em><strong>Objective(s):</strong></em> Dopamine plays an important role in cognitive functions. Inhibition of the dopamine-degrading enzyme catechol-O-methyltransferase (COMT) may have beneficial effects. Our aim was to assess the effect of COMT inhibitor tolcapone (TCP) on learning and memory in naïve and haloperidol-challenged rats.<br /><em><strong>Materials and Methods:</strong></em> Male Wistar rats were divided into 9 groups (n=8): naïve-saline, tolcapone 5; 15 and 30 mg/kg BW; haloperidol (HP) challenged-saline, haloperidol, haloperidol+tolcapone 5; 15 and 30 mg/kg BW. Two-way active avoidance test (TWAA), elevated T-maze, and activity cage were performed. Observed parameters were: number of conditioned responses (CR) and unconditioned responses (UCR), working memory index, and vertical and horizontal movements. <br /><em><strong>Results:</strong></em> Naïve rats with 30 mg/kg BW TCP had a significantly increased number of CR and UCR during the long-term memory test. The animals with 5 mg/kg BW TCP significantly increased the number of UCR during the two retention tests. In haloperidol-challenged rats, the three experimental groups decreased the number of CR and UCR during the learning session and the two memory tests, compared to the saline group. There was no significant difference between the HP-challenged rats treated with TCP and the haloperidol control group. All experimental naïve groups had significantly increased working memory index whereas none of the HP-challenged groups showed significant increase in this parameter. <br /><em><strong>Conclusion:</strong></em> Our results demonstrate that in naïve rats tolcapone improves memory in the hippocampal-dependent TWAA task and spatial working memory in T-maze.
COMT,Dopamine,Hippocampus,Prefrontal cortex,Spatial Memory,Tolcapone,Working memory
https://ijbms.mums.ac.ir/article_12772.html
https://ijbms.mums.ac.ir/article_12772_6f563c6beb866d7ca985ae3cf4d7ddbc.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
22
6
2019
06
01
Long-term oral intake of Panax ginseng improves hypomagnesemia, hyperlactatemia, base deficit, and metabolic acidosis in an alloxan-induced rabbit model
703
709
EN
Gareeballah
Osman Adam
0000-0002-8650-8117
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Chonbuk National University, Iksan Campus, 79 Gobong-ro, Iksan-si, Jeollabuk-do 54596 Republic of Korea
gorba000@gmail.com
Gi-Beum
Kim
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Chonbuk National University, Iksan Campus, 79 Gobong-ro, Iksan-si, Jeollabuk-do 54596 Republic of Korea
kgb70@jbnu.ac.kr
Sei-Jin
Lee
Korea Basic Science Institute Jeonju Centre, Deokjin gu, Jeonju-si, Jeollabuk do 54896, Republic of Korea
lsj@kbsi.re.kr
Hee-Ryung
Lee
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Chonbuk National University, Iksan Campus, 79 Gobong-ro, Iksan-si, Jeollabuk-do 54596 Republic of Korea
20155485@jbnu.ac.kr
Shang-Jin
Kim
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Chonbuk National University, Iksan Campus, 79 Gobong-ro, Iksan-si, Jeollabuk-do 54596 Republic of Korea
abbasj@jbnu.ac.kr
Hyng-Sub
Kang
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Chonbuk National University, Iksan Campus, 79 Gobong-ro, Iksan-si, Jeollabuk-do 54596 Republic of Korea
kang-hs@jbnu.ac.kr
Jin-Shang
Kim
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Chonbuk National University, Iksan Campus, 79 Gobong-ro, Iksan-si, Jeollabuk-do 54596 Republic of Korea
kimjs@jbnu.ac.kr
10.22038/ijbms.2019.33223.7936
<em><strong>Objective(s):</strong></em> Panax ginseng (PG) widely used for its various pharmacological activities, including effects on diabetes and its complications. This study aims to investigate the effect of PG on mortality-related hypomagnesemia, hyperlactatemia, metabolic acidosis, and other diabetes-induced abnormalities. <br /><em><strong>Materials and Methods:</strong></em> Type 1 diabetes was induced by IV injection of alloxan monohydrate 110 mg/kg into New Zealand white rabbits weighing 2-2.5 kg. PG was supplied in drinking water for 20 weeks. The effects of the PG treatment on diabetes were evaluated through hematological and biochemical analysis including ELISA assays for insulin and glycated haemoglobin A1c (HBA1c) before and after PG extract was supplied. <br /><em><strong>Results:</strong></em> The serum glucose, insulin, and HBA1c levels were significantly improved after the PG treatment compared to those found before PG treatment. In addition, Mg2+, lactate, and base deficit, and acidosis was significantly enhanced in treated rabbits. Moreover, PG showed hepato- and renoprotective effect. Likewise, electrolytes, lipid and protein profile were improved.<br /><em><strong>Conclusion:</strong></em> The biochemical and hematological analysis data demonstrate that the PG is effective to alleviate the diabetes serious signs.
Acidosis,Glycated hemoglobin A1c,Hyperlactatemia,Hypomagnesemia,Panax ginseng,Type 1 diabetes
https://ijbms.mums.ac.ir/article_12798.html
https://ijbms.mums.ac.ir/article_12798_90c8e2fe8a31691beeb652841da5db4f.pdf