Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
3
2021
03
01
Planning and management to control and eliminate HTLV-1 infection in Iran
264
266
EN
Reza
Boostani
0000-0001-7998-9592
Department of Neurology, Mashhad University of Medical Sciences, Mashhad, Iran
boostanir@mums.ac.ir
Nasim
Lotfinejad
0000-0001-8491-9900
Department of Research, Faculty of Medicine, Mashhad University of medical sciences, Mashhad, Iran
nasim.lotfinezhad@gmail.com
Fariba
Zemorshidi
Department of Neurology, Mashhad University of Medical Sciences, Mashhad, Iran
zemorshidif@mums.ac.ir
Zohreh
Vahidi
Immunology Research Centre, Inflammation and Inflammatory Diseases Division, Mashhad University of Medical Sciences, Mashhad, Iran
ze.vahidi@gmail.com
Seyed Abdolrahim
Rezaee
0000-0001-6814-5992
Immunology Research Centre, Inflammation and Inflammatory Diseases Division, Mashhad University of Medical Sciences, Mashhad, Iran
rezaeer@mums.ac.ir
Reza
Farid
Allergy Research Centre, Mashhad University of Medical Sciences, Mashhad, Iran
rfaridh@gmail.com
Houshang
Rafatpanah
0000-0002-1061-5864
Immunology Research Centre, Inflammation and Inflammatory Diseases Division, Mashhad University of Medical Sciences, Mashhad, Iran
rafatpanahh@mums.ac.ir
10.22038/ijbms.2021.50803.11562
Prevention and treatment of the Human T-cell leukemia virus, type 1 (HTLV-1) which was discovered nearly 40 years ago, still remain challenging. The reported high prevalence of HTLV-1 in some countries around the world triggered an open letter to the World Health Organization (WHO), urging action against HTLV-1 infection in 2018. This highlights the importance of virus elimination strategies to eradicate HTLV-1 infection. In Iran, we have documented our experiences with the virus in order to achieve and promote the possible ways to manage, control, and eliminate HTLV-1. Although there has been considerable progress apropos of HTLV-1, a series of additional challenges need to be tackled to control HTLV-1 infection in Iran.
ATL HAM/TSP HTLV,1 Prevention Treatments
https://ijbms.mums.ac.ir/article_17601.html
https://ijbms.mums.ac.ir/article_17601_299e308ee1eb3c7760033c0139df391f.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
3
2021
03
01
MicroRNAs may provide new strategies in the treatment and diagnosis of diabetic retinopathy: Importance of VEGF
267
279
EN
Vahid
Akbari Korhkheyli
0000-0002-6233-9726
Department of Clinical Biochemistry and Medical Genetics, Student Research Committee, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
akbari.vahid89@gmail.com
Mohammad Amir
Mishan
0000-0001-8210-9322
Ocular Tissue Engineering Research Center, Research Institute for Ophthalmology and Vision Science, Shahid Beheshti University of Medical Sciences, Tehran, Iran
mo.am.mishan@gmail.com
Abbas
Khonakdar Tarsi
0000-0002-8630-1722
Department of Clinical Biochemistry and Medical Genetics, Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
khonakdarab@gmail.com
Abdolkarim
Mahrooz
Department of Clinical Biochemistry and Medical Genetics, Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
amahrooz@mazums.ac.ir
Mozhgan
Rezaei Kanavi
Ocular Tissue Engineering Research Center, Research Institute for Ophthalmology and Vision Science, Shahid Beheshti University of Medical Sciences, Tehran, Iran
rezaeikanavi@gmail.com
Ali
Hafezi-Moghadam
0000-0002-5336-0697
Molecular Biomarkers Nano-Imaging Laboratory, Brigham and Women’s Hospital, Boston, Massachusetts, USA
ahm@bwh.harvard.edu
Abouzar
Bagheri
0000-0001-8040-5054
Department of Clinical Biochemistry and Medical Genetics, Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
a.bagherimg@gmail.com
10.22038/ijbms.2021.52164.11807
Diabetic retinopathy (DR) is ocular microvascular complications of diabetes mellitus. Along with the increasing prevalence of diabetes worldwide, DR has come into the major cause of human blindness. Several studies have demonstrated the important roles of the expression alteration in the proteins contributed to vascular dysfunction during DR, especially vascular endothelial growth factor (VEGF). However, there is a need for further mechanistic research in this context to design new therapeutic and diagnostic programs. MicroRNAs (miRNAs, miRs) have been introduced as key controllers of gene expression in a variety of biological processes including differentiation, proliferation, and metabolism. Altered expression of miRNAs during DR development indicates a close relationship between these regulatory molecules and DR through regulating gene expressions. This review discusses and updates the functions of miRNA-dependent pathways and key roles of VEGF in the DR, which may increase our understanding and ability to target these small but important molecules to efficiently improve therapeutic and diagnostic approaches.
Diabetic retinopathy,Gene regulation,Molecular targeting,miRNAs,VEGF
https://ijbms.mums.ac.ir/article_17398.html
https://ijbms.mums.ac.ir/article_17398_7272f622378d4f5afb611dd9e3af66e7.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
3
2021
03
01
The protective effect of Azadirachta indica (neem) against metabolic syndrome: A review
280
292
EN
Fatemeh
Yarmohammadi
0000-0002-0552-8766
Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran
yarmohammadif971@mums.ac.ir
Soghra
Mehri
0000-0002-9697-2343
Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
mehris@mums.ac.ir
Nahid
Najafi
Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran
najafin971@mums.ac.ir
Sanaz
Salar amoli
0000-0001-7140-772X
Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran
salaramolis971@mums.ac.ir
Hossein
Hosseinzadeh
0000-0002-3483-851X
Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
hosseinzadehh@mums.ac.ir
10.22038/ijbms.2021.48965.11218
Metabolic syndrome is a condition associated with obesity, diabetes, dyslipidemia, and high blood pressure. Recently, the use of phytochemicals is suggested in the control and treatment of metabolic syndrome. The Azadirachta indica (neem) is an evergreen tree belonging to the family of Meliaceae. Multiple studies have been confirmed the anti-diabetic and anti-hypertension, anti-hyperlipidemia, and anti-obesity effects of neem. In this review, we reported the protective effects of neem against the complications of metabolic syndrome with a special focus on mechanisms that are involved. It has been shown that neem can control hyperglycemia and hypertension through over-expression of transcription factor nuclear factor erythroid 2–related factor 2 (Nrf2) and anti-oxidant effects. Neem also reduced the glucose uptake through up-regulation of glucose transporter 4 (GLUT4) and inhibition of key intestinal enzymes such as glucosidases. Moreover, neem showed anti-hypertensive effects possibility via the block of calcium channels, up-regulation of endothelial nitric oxide synthase (eNOS), and extracellular signal-regulated kinases 1/2 (ERK1/2) signaling pathway. Anti-oxidant effects play an important role in protective mechanisms of neem against metabolic syndrome and its complications.
Azadirachta indica,Diabetes,Hyperlipidemia,Hypertension,metabolic syndrome,Neem,Obesity
https://ijbms.mums.ac.ir/article_17397.html
https://ijbms.mums.ac.ir/article_17397_fafb960c3288721f00b352dd55b90755.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
3
2021
03
01
Thiamine reduced metabolic syndrome symptoms in rats via down-regulation of hepatic nuclear factor-kβ and induction activity of glyoxalase-I
293
299
EN
Sina
Mahdavifard
0000-0001-9878-2984
Department of Clinical Biochemistry, Ardabil University of Medical Sciences, Ardabil, Iran
mahdavifards@yahoo.com
Razieh
Dehghani
Department of Clinical Biochemistry, Ardabil University of Medical Sciences, Ardabil, Iran
razieh_deg@gmail.com
Farhad
Jeddi
Department of Genetics and Pathology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
jeddi-f@yahoo.com
Nowruz
Najafzadeh
Research Laboratory for Embryology and Stem Cells, Department of Anatomical Sciences, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
nj1355@gmail.com
10.22038/ijbms.2021.53707.12086
<em><strong>Objective(s):</strong></em> Metabolic syndrome (MS) is a cause of death worldwide. The hepatic nuclear factor- NF-kβ (NF-kβ) is the cardinal player of hepatic homeostasis, insulin sensitivity, and lipid metabolism. Thus, we investigated the effect of thiamine on hepatic gene expression of NF-kβ and its levels of activators in MS rats. <br /><em><strong>Materials and Methods:</strong></em> Male Wistar rats were randomly divided into 4 equal groups (ten rats in each group): normal, MS, and two alike groups under thiamine treatment. MS was induced in rats with a high sucrose solution (40 % in drinking water) for 4 months. Treated groups of rats received 0.18 % of thiamine daily in drinking water. Hematoxylin-Eosin stains were employed to determine the histopathological changes of the liver. Metabolic profile, glycation products, oxidative stress, inflammatory markers, the activity of glyoxalase-I, as well as NF-kβ hepatic expression of all rat groups, were determined.<br /><em><strong>Results:</strong> </em>Acute hepatitis was not observed in the livers of the thiamine treated MS rats. Besides, the treatment showed an advantageous effect on glucose, lipid metabolism, and body weight via down-regulation of hepatic NF-kβ and induction of glyoxalase system activity. Furthermore, the treatment decreased diverse glycation, oxidative stress, and inflammatory markers (P>0.001). <br /><em><strong>Conclusion:</strong></em> Thiamine decreased body weight and improved metabolism and activity of glyoxalase-I in MS rats with anti-glycation, antioxidant, and anti-inflammatory activities. Further, the treatment had a hepato-protective effect via reduction of NF-kβ signaling.
Glycation Glyoxalase,I Metabolic syndrome Nuclear factor,kβ Thiamine
https://ijbms.mums.ac.ir/article_17457.html
https://ijbms.mums.ac.ir/article_17457_0558edb281899b41363ff79a2a60d7fe.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
3
2021
03
01
Favorable effects of dill tablets and Ocimum basilicum L. extract on learning, memory, and hippocampal fatty acid composition in hypercholesterolemic rats
300
311
EN
Neda
Heshami
0000000182841722
Department of Clinical Biochemistry, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
n.heshami915@gmail.com
Soheila
Mohammadali
Department of Clinical Biochemistry, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
mohammadali.soheila@gmail.com
Alireza
Komaki
Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
komaki@umsha.ac.ir
Heidar
Tayebinia
0000-0002-7243-660X
Department of Clinical Biochemistry, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
tavilani@gmail.com
Jamshid
Karimi
0000-0001-8253-7124
Department of Clinical Biochemistry, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
jamshidkarimi2013@gmail.com
Ebrahim
Abbasi Oshaghi
Department of Clinical Biochemistry, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
7abbasi@gmail.com
Mohammad
Hashemnia
0000-0002-2899-4794
Department of Pathobiology, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran
m.hashemnia@razi.ac.ir
Iraj
Khodadadi
0000000190484528
Department of Clinical Biochemistry, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
khodadadi@umsha.ac.ir
10.22038/ijbms.2021.49013.11230
<em><strong>Objective(s):</strong></em> Hypercholesterolemia is correlated with brain amyloid-β (Aβ) deposition and impaired cognitive functions and contributes to Alzheimer’s disease. Effects of cholesterol-lowering dill tablets and aqueous extract of Ocimum basilicum L. (basil) on learning and memory and hippocampus fatty acid composition were examined. mRNA levels of the genes involved in cholesterol homeostasis were also determined in high-cholesterol diet (HCD) fed rats. <br /><em><strong>Materials and Methods:</strong></em> Forty male Wistar rats were allocated to 4 groups: rats fed chow diet (C); rats fed high-cholesterol (2%) diet (HCD); rats treated with HCD+300 mg/kg dill tablets (HCD+Dill); and finally, rats fed HCD and treated with 400 mg/kg basil aqueous extract (HCD+basil). Treatment was carried out for 16 weeks. Hippocampus Aβ(1-42) level was determined. Spatial and passive avoidance tests were used to examine cognitive functions. Hippocampal FA composition was assessed by gas chromatography. Basil aqueous extract was analyzed by GC-double mass spectroscopy (GC-MS/MS) and expression of LXR-α, LXR-β, and ABCA1 genes was assessed by qRT-PCR. <br /><em><strong>Results:</strong></em> Dill tablets and basil extract remarkably ameliorated serum cholesterol (p <0.001), retarded hippocampal accumulation of Aβ, and attenuated HCD-induced memory impairment. Hippocampus FA composition did not change but serum cholesterol was found positively correlated with hippocampus Aβ(1-42) (p <0.001), total n 6 PUFA (P=0.013), and Aβ(1-42) showed correlation with the ratio of n6 to n3 PUFA. At least 70 components were identified in basil aqueous extract. <br /><em><strong>Conclusion:</strong></em> Dill tablets and aqueous extract of basil attenuated the hypercholesterolemia-induced memory impairment by lowering serum cholesterol and hippocampus amyloid deposits, and probably beneficial in AD adjuvant therapy.
Alzheimer’s disease,Dill,Hypercholesterolemia,Learning,Memory,Ocimum
https://ijbms.mums.ac.ir/article_17501.html
https://ijbms.mums.ac.ir/article_17501_4b11c08e3958a71cfc7f1cc909f9f356.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
3
2021
03
01
Acrylamide exposure aggravates the development of ulcerative colitis in mice through activation of NF-κB, inflammatory cytokines, iNOS, and oxidative stress
312
321
EN
Keyvan
Amirshahrokhi
0000-0001-6761-1736
Department of Pharmacology, School of Pharmacy, Ardabil University of Medical Sciences, Ardabil, Iran
amirshahrokhi@gmail.com
10.22038/ijbms.2021.52233.11816
<em><strong>Objective(s):</strong></em> Acrylamide is a toxic compound that forms during food processing at high temperatures. Acrylamide has been shown to induce toxicity in various organs in the body. This study aimed to investigate the effect of acrylamide exposure on the susceptibility of the colon to ulcerative colitis in a mouse model. <br /><em><strong>Materials and Methods:</strong></em> Mice were pretreated with acrylamide (oral, 20 and 30 mg/kg/day) for 21 consecutive days, and colitis was induced by intrarectal administration of acetic acid. <br /><em><strong>Results:</strong></em> The results revealed that acrylamide-pretreatment significantly increased disease activity index (DAI), macroscopic damage, histological changes of the colonic mucosa and oxidative stress markers carbonyl protein, malondialdehyde (MDA), and nitric oxide (NO), whereas it decreased the levels of anti-oxidants glutathione (GSH), superoxide dismutase (SOD) and catalase. Moreover, induction of colitis in acrylamide-pretreated mice caused a higher increase in colonic levels of myeloperoxidase (MPO), matrix metalloproteinase (MMP)-9, monocyte chemoattractant protein (MCP)-1, cytochrome-c, caspase-3, proinflammatory cytokine tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-1β, and interferon (IFN)-γ, whereas it reduced the level of IL-10. The mRNA expression of nuclear factor kappa B (NF-κB) and inducible nitric oxide synthase (iNOS) were further increased in colon tissue of mice exposed to acrylamide.<br /><em><strong>Conclusion:</strong></em> These findings suggest that acrylamide can accelerate the development of acetic acid-induced colitis. In conclusion, chronic acrylamide exposure may aggravate the severity of ulcerative colitis and increase colonic mucosal damage through oxidative stress and inflammatory responses.
Acrylamide,Apoptosis,Cytokines,Inflammation,Oxidative stress,Ulcerative colitis
https://ijbms.mums.ac.ir/article_17502.html
https://ijbms.mums.ac.ir/article_17502_e7502978fc757c9384ad91aa956416e4.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
3
2021
03
01
Protective effects of selenium on electromagnetic field-induced apoptosis, aromatase P450 activity, and leptin receptor expression in rat testis
322
330
EN
Sareh
Khoshbakht
0000-0001-6192-9188
Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
khoshbakhts961@mums.ac.ir
Fatemeh
Motejaded
0000-0001-9257-4789
Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
motejadedf1@mums.ac.ir
Sareh
Karimi
0000-0001-8938-4749
Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
karimisareh@yahoo.com
Narjes
Jalilvand
0000-0002-0203-3039
Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
jalilvandn1981@gmail.com
Alireza
Ebrahimzadeh-bideskan
0000-0003-4333-250X
Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
ebrahimzadehba@mums.ac.ir
10.22038/ijbms.2021.45358.10554
<em><strong>Objective(s):</strong></em> Electromagnetic field (EMF) emitted by mobiles may affect the male reproductive system. Selenium, as an antioxidant, may protect against electromagnetic field-induced tissue damage. Theis study aimed to investigate the effects of selenium on rat testis exposed to electromagnetic fields.<br /><em><strong>Materials and Methods:</strong></em> Twenty-four male Wistar rats were divided into four groups, namely EM group (2100 MHZ), EM/SE group (2100 MHZ + selenium (0.2 mg/kg), SE group (selenium 0.2 mg/kg), CONT (control group). Serum LH, FSH, testosterone, leptin and aromatase levels, testis weight and volume index, sperm parameters (count and abnormal percent), seminiferous tubule diameters, germinal epithelia thickness, immunoreactivity of leptin receptor and caspase-3 (for apoptotic cells in germinal epithelium) were investigated.<br /><em><strong>Results:</strong></em> Our results showed that serum LH, FSH, GnRH, testosterone level, sperm count, germinal epithelium thickness, and seminiferous tubule diameter were significantly declined in the EM group compared with the CONT group (p <0.05). However, in the EM group, the serum leptin level, sperm abnormality, aromatase enzyme level, apoptotic cells, and leptin receptor were increased compared with the CONT group (p <0.05). Furthermore, an increase in sperm count, germinal epithelium thickness, seminiferous diameters, serum LH, FSH, and GnRH, and testosterone levels, and a significant decrease in sperm abnormality, leptin receptor and apoptotic cells in the EM/SE group compared with the EM group were also observed (p <0.05). <br /><em><strong>Conclusion:</strong></em> This study showed that electromagnetic radiation may have detrimental impacts on the male reproductive system, which can be prevented by use of selenium.
Apoptosis,Electromagnetic Radiation,Leptin receptor,Selenium,Testis
https://ijbms.mums.ac.ir/article_17455.html
https://ijbms.mums.ac.ir/article_17455_283b0ac5b2b057550b597f9da1a91bff.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
3
2021
03
01
Sustained release of linezolid in ocular insert based on lipophilic modified structure of sodium alginate
331
340
EN
Ashkan
Mohammad Sadeghi
0000-0001-6799-4335
Department of Quality Control, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
ashkan_msadghi@yahoo.com
Fatemeh
Farjadian
0000-0002-2093-0454
Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
fatemehfarjadian@gmail.com
Shohreh
Alipour
0000-0002-4071-9031
Department of Quality Control, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
alipour_sh@sums.ac.ir
10.22038/ijbms.2021.49866.11385
<em><strong>Objective(s):</strong></em> Ocular inserts are usually polymeric thin films with increased ocular residence time and sustained drug release capacity. Sodium alginate is a biocompatible and biodegradable carrier; however, initial burst release of encapsulated drug within it, is recognized as a challenge. Grafting –addition of functional moieties to a polymer– is a technique to modify polymers’ physicochemical properties, including higher ability to control drug release. Linezolid (LNZ) solution is used in consecutive doses in treatment of antibiotic-resistant Gram-positive bacterial infections especially induced by methicillin resistant Staphylococcus aureus (MRSA). <br /><em><strong>Materials and Methods:</strong></em> Grafted alginate copolymers were synthesized using butyl methacrylate (BMC) and lauryl methacrylate (LMC) at two different reaction times (12 hr and 24 hr). Copolymerization was evaluated by 1H-NMR, Ft-IR, and TGA. Copolymer safety was examined by cytotoxicity test against HEK-293 cell. Linezolid inserts were prepared using optimized copolymers and characterized.<br /><em><strong>Results:</strong></em> 1H-NMR, Ft-IR, and TGA confirmed the successful grafting of alginate copolymers. ALG-B24 and ALG-L12 showed the highest safety against HEK-293 cell line comparing with intact alginate. Linezolid insert characterization results indicated a slower linezolid release profile related to creation of a lipophilic structure. A better strength property for linezolid loaded ALG-B24 and ALG-L12 inserts was obtained while ALG-L12 showed a stronger adhesive force compared with intact alginate. Antibacterial efficacy on clinical isolated MRSA after 24 hr was similar to linezolid solution.<br /><em><strong>Conclusion:</strong></em> Lipophilic alginate copolymer (ALG-L12) showed a sustained release capability while retaining its main feature in strong film forming ability so it seems to be a promising safe carrier.
Copolymer,Grafting,Linezolid,Ocular insert,Sodium alginate
https://ijbms.mums.ac.ir/article_17456.html
https://ijbms.mums.ac.ir/article_17456_ae10e234d43c756b1fa2e5e043fb35d4.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
3
2021
03
01
Preventive electroacupuncture ameliorates D-galactose-induced Alzheimer’s disease-like inflammation and memory deficits, probably via modulating the microbiota–gut–brain axis
341
348
EN
Chuan
He
760237205@qq.com
College of Acupuncture and Orthopedics, Hubei University of Chinese Medicine, Wuhan, Hubei, China
760237205@qq.com
Zhong-Sheng
Huang
College of Acupuncture and Orthopedics, Hubei University of Chinese Medicine, Wuhan, Hubei, China
2479380476@qq.com
Chao-Chao
Yu
kiterunner2@163.com
Department of Tuina, Shenzhen Traditional Chinese Medicine Hospital
kiterunner2@163.com
Xue-Song
Wang
College of Acupuncture and Orthopedics, Hubei University of Chinese Medicine, Wuhan, Hubei, China
Tao
Jiang
College of Acupuncture and Orthopedics, Hubei University of Chinese Medicine, Wuhan, Hubei, China
jtjt661@sina.com
Miao
Wu
4806599@qq.com
Hubei Provincial Hospital of TCM, Wuhan, Hubei, China
4806599@qq.com
Li-Hong
Kong
0000-0002-4287-0536
College of Acupuncture and Orthopedics, Hubei University of Chinese Medicine, Wuhan, Hubei, China
xiyu1618@sina.com
10.22038/ijbms.2021.49147.11256
<em><strong>Objective(s):</strong></em> We aimed to observe the effects of preventive electroacupuncture (EA) on the microbiota–gut–brain axis and spatial learning and memory deficits and to investigate the possible mechanism using D-galactose (D-gal)-induced aging rats. <br /><em><strong>Materials and Methods:</strong></em> D-gal was intraperitoneally injected to establish the aging model. We used Morris water maze to detect spatial learning and memory function of rats. RT-PCR was applied to test targeted gut microbes. The expression of zonula occludens-1 (ZO-1) and Toll-like receptor 4 (TLR4)/nuclear factor (NF)-κB pathway proteins were detected by Western blotting. ELISA was employed to evaluate the level of lipopolysaccharides (LPS), diamine oxidase (DAO) and S-100β. Additionally, we observed ionized calcium-binding adapter molecule-1 (Iba-1) expression in the hippocampal CA1 area by immunofluorescence.<br /><em><strong>Results:</strong></em> Morris water maze test showed decreased mean escape latency and increased target quadrant time after EA treatment. The gut microbiota composition has been modified in EA treated rats. Molecular examination indicated that expression of ZO-1 was improved and the the concentration of LPS in blood and hippocampus were reduced in EA treated rats. Further, we observed an inhibition of activated microglia and TLR4/NF-κB pathway in EA groups. <br /><em><strong>Conclusion:</strong></em> Preventive EA may alleviate the impairments of the microbiota–gut–brain axis and spatial learning and memory in aging, and the mechanism may be related to the inhibition of TLR4/NF-kB signaling pathway. The combination of acupoints GV20 and ST36 can enhance the therapeutic effect in aging rats.
Aging Alzheimer’s disease Electroacupuncture Microbiota,Gut,brain axis NF,κB TLR4
https://ijbms.mums.ac.ir/article_17607.html
https://ijbms.mums.ac.ir/article_17607_edc4936d24ab85e0bc1dfd2242db9256.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
3
2021
03
01
Improved spatial memory, neurobehavioral outcomes, and neuroprotective effect after progesterone administration in ovariectomized rats with traumatic brain injury: Role of RU486 progesterone receptor antagonist
349
359
EN
Ladan
Amirkhosravi
0000-0001-9174-1426
Neuroscience Research and Physiology Research Centers, Kerman University of Medical Sciences, Kerman, Iran
ladan.amirkhosravi@gmail.com
Mohammad
Khaksari
0000-0003-0770-4281
Endocrinology and Metabolism Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran
mkhaksari@kmu.ac.ir
Vahid
Sheibani
Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran
vsheibani@yahoo.com
Nader
Shahrokhi
Physiology Research Centers, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran
nshahrokhisa@yahoo.com
Mohammad navid
Ebrahimi
Neuroscience Research and Physiology Research Centers, Kerman University of Medical Sciences, Kerman, Iran
bsuk.navid@gmail.com
Sedigheh
Amiresmaili
0000-0002-7158-4096
Department of Physiology, Bam University of Medical Sciences, Bam, Iran
esmailisedigheh@yahoo.com
Neda
Salmani
Department of Psychology, Genetic Institute, Islamic Azad University- Zarand Branch, Kerman, Iran
ne_salmani@yahoo.com
10.22038/ijbms.2021.50973.11591
<em><strong>Objective(s):</strong></em> The contribution of classic progesterone receptors (PR) in interceding the neuroprotective efficacy of progesterone (P4) on the prevention of brain edema and long-time behavioral disturbances was assessed in traumatic brain injury (TBI). <br /><em><strong>Materials and Methods:</strong></em> Female Wistar rats were ovariectomized and apportioned into 6 groups: sham, TBI, oil, P4, vehicle, and RU486. P4 or oil was injected following TBI. The antagonist of PR (RU486) or DMSO was administered before TBI. The brain edema and destruction of the blood-brain barrier (BBB) were determined. Intracranial pressure (ICP), cerebral perfusion pressure (CPP), and beam walk (BW) task were evaluated previously and at various times post-trauma. Long-time locomotor and cognitive consequences were measured one day before and on days 3, 7, 14, and 21 after the trauma. <br /><em><strong>Results:</strong></em> RU486 eliminated the inhibitory effects of P4 on brain edema and BBB leakage (p <0.05, p <0.001, respectively). RU486 inhibited the decremental effect of P4 on ICP as well as the increasing effect of P4 on CPP (p <0.001) after TBI. Also, RU486 inhibited the effect of P4 on the increase in traversal time and reduction in vestibulomotor score in the BW task (p <0.001). TBI induced motor, cognitive, and anxiety-like disorders, which lasted for 3 weeks after TBI; but, P4 prevented these cognitive and behavioral abnormalities (p <0.05), and RU486 opposed this P4 effect (p <0.001).<br /> <em><strong>Conclusion:</strong></em> The classic progesterone receptors have neuroprotective effects and prevent long-time behavioral and memory deficiency after brain trauma.
Behavioral disorders,Mifepristone,Neuroprotection,Progesterone,Spatial Memory,TBI
https://ijbms.mums.ac.ir/article_17461.html
https://ijbms.mums.ac.ir/article_17461_aa211a998b5641288b9f79a275600712.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
3
2021
03
01
Designing and generating a single-chain fragment variable (scFv) antibody against IL2Rα (CD25): An in silico and in vitro study
360
368
EN
Parnian
Navabi
0000-0002-4444-3650
Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
parniannvb@gmail.com
Mohamad Reza
Ganjalikhany
Department of Cell and Molecular Biology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran
ganjalikhany@gmail.com
Sepideh
Jafari
Department of Cell and Molecular Biology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran
sepidejafari71@gmail.com
Moein
Dehbashi
Division of Genetics, Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Sciences and Technologies, University of Isfahan, Isfahan, Iran
modehbashi110@yahoo.com
Mazdak
Ganjalikhani-Hakemi
0000-0002-4764-7616
Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
mghakemi@med.mui.ac.ir
10.22038/ijbms.2021.51709.11728
<em><strong>Objective(s):</strong></em> IL-2Rα plays a critical role in maintaining immune function. However, expression and secretion of CD25 in various malignant disorders and autoimmune diseases are now well established. Thus, CD25 is considered an important target candidate for antibody-based therapy. This study aimed to find the most suitable linker peptide to construct a functional anti-CD25 single-chain fragment variable (scFv) by bioinformatics studies and its production in a bacterial expression system. <br /><em><strong>Materials and Methods:</strong></em> Here, the 3D structures of the scFvs with different linkers were predicted and molecular dynamics simulation was performed to compare their structures and dynamics. Then, interactions between five models of scFv and human CD25 were calculated via molecular docking. According to MD and docking results, the anti-CD25 scFvs with (Gly4Ser)3 linker were constructed and cloned into pET-22b(+). Then, recombinant plasmids were transformed into Escherichia coli Bl21 (DE3) for expression using IPTG and lactose as inducers. Anti-CD25 scFv was purified from the periplasm and detected by SDS-PAGE and Western blot. Afterward, functionality was evaluated using ELISA.<br /><em><strong>Results:</strong></em> In silico analysis showed that the model containing (Gly4Ser)3 as a linker has more stability compared with other linkers. The results of SDS-PAGE, Western blot, and ELISA confirmed the accuracy of anti-CD25 scFv production and its ability to bind to the human CD25. <br /><em><strong>Conclusion:</strong></em> Conclusively, our work provides a theoretical and experimental basis for production of an anti-CD25 scFv, which may be applied for various malignant disorders and autoimmune diseases.
CD25 Daclizumab IL,2Rα Protein engineering Single,chain variable fragment (scFv)
https://ijbms.mums.ac.ir/article_17460.html
https://ijbms.mums.ac.ir/article_17460_db8c2bc6b13c55f042ef0d00e82b3302.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
3
2021
03
01
c-kit+ cells offer hopes in ameliorating asthmatic pathologies via regulation of miRNA-133 and -126
369
376
EN
Reza
Rahbarghazi
0000-0003-3864-9166
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
rezarahbardvm@gmail.com
Rana
Kihanmanesh
0000-0002-6941-2690
Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
rkeyhanmanesh@gmail.com
Jafar
Rezaie
Solid Tumor Research Center, Research Institute for Cellular and Molecular Medicine, Urmia University of Medical Sciences, Urmia, Iran
j.rezaie88@gmail.com
Fatemeh
Mirershadi
0000-0002-3221-1780
Department of Physiology, Ardabil Branch, Islamic Azad University, Ardabil, Iran
fmirershadi@yahoo.com
Hossain
Heiran
Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
hossin.heiran@gmail.com
Hesam
Saghaei Bagheri
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
hs.sbageri@gmail.com
Shirin
Saberianpour
https://orcid.org/0000-0001-5546-5823
Vascular and Endovascular Surgery Research Center, Mashhad University of Medical Science, Mashhad, Iran
saberi_shirin@yahoo.com
Aysa
Rezabakhsh
Physical Medicine and Rehabilitation Research Center, Aging Research Institute, Tabriz University of Medical Sciences, Tabriz, Iran
aysapharma.rezabakhsh@gmail.com
Aref
Delkhosh
0000-0003-3489-9934
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
arefdelkhosh@yahoo.com
Yasin
Bagheri
Young Researchers and Elite Club, Tabriz Branch Islamic Azad university, Tabriz, Iran
dr.b_iaut@yahoo.com
Hadi
Rajabi
0000-0001-6086-591X
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
hadirajabi.bio@gmail.com
Mahdi
Ahmadi
0000-0002-0774-9378
Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
mahdi59866@gmail.com
10.22038/ijbms.2021.49008.11231
<em><strong>Objective(s):</strong></em> There are still challenges regarding c-kit+ cells’ therapeutic outcome in the clinical setting. Here, we examined the c-kit+ cell effect on the alleviation of asthma by modulating miRNAs expression.<br /><em><strong>Materials and Methods:</strong></em> To induce asthma, male rats were exposed to ovalbumin. Bone marrow-derived c-kit+ cells were enriched by MACS. Animals were classified into four groups (6 rats each). Control rats received PBS intratracheally; Ovalbumin-sensitized rats received PBS intratracheally; Ovalbumin-sensitized rats received PBS intratracheally containing 3×105 c-kit+ and c-kit- cells. Cells were stained with Dil fluorescent dye to track in vivo condition. Pathological changes were monitored in asthmatic rats after transplantation of c-kit+ and c-kit- cells. Serum levels of IL-4 and INF-γ were measured by ELISA. Transcription of miRNAs (-126 and 133) was assessed by real-time PCR analysis.<br /><em><strong>Results:</strong></em> Pathological examination and Th1 and Th2 associated cytokine fluctuation confirmed the occurrence of asthma in rats indicated by chronic changes and prominent inflammation compared with the control group (p <0.05). Both c-kit+ and c-kit- cells were verified in pulmonary niche. Administration of c-kit positive cells had the potential to change INF-γ/IL-4 ratio close to the normal values compared with matched-control asthmatic rats (p <0.05). We also found that c-kit+ cells regulated the expression of miRNA-126 and -133, indicated by an increase of miRNA-133 and decrease of miRNA-126 compared with cell-free sensitized groups (p <0.05). <br /><em><strong>Conclusion:</strong></em> c-kit- cells were unable to promote any therapeutic outcomes in the asthmatic milieu. c-kit+ cells had the potential to diminish asthma-related pathologies presumably by controlling the transcription of miRNA-126 and -133.
Cell therapy,Histological changes,Lung,Ovalbumin,Rat
https://ijbms.mums.ac.ir/article_17462.html
https://ijbms.mums.ac.ir/article_17462_81e697fef497e998e46655954d97af6d.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
3
2021
03
01
Immunogenicity evaluation of the HIV-1 Tat containing polyepitope DNA vaccine adjuvanted with CpG-ODNs in mice
377
382
EN
Ehsan
Ollah
Jazaeri
0000-0003-1369-4139
Department of Biological Sciences, Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan, Iran
e.jazaeri@iasbs.ac.ir
Atiyeh
Mahdavi
0000-0002-6715-4165
Department of Biological Sciences, Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan, Iran
a.mahdavi@iasbs.ac.ir
Asghar
Abdoli
Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran
a_abdoli@pasteur.ac.ir
10.22038/ijbms.2021.52890.11923
<em><strong>Objective(s):</strong></em> HIV-1 is still considered a serious threat to human health, and accessibility of a suitable and efficient vaccine is urgently needed to address the disease burden. DNA vaccines employ the cells of the vaccinated hosts for in situ production of the vaccines. This strategy is an alternative and effective approach for traditional vaccination against high-risk pathogens, e.g., HIV-1. On the other hand, polyepitope vaccines, containing several immunogenic and conserved epitopes from virus vital regulatory and structural proteins, could more efficiently induce cellular and humoral immune responses against different clades of the virus. <br /><em><strong>Materials and Methods:</strong></em> Herein, we comparatively investigated the immunogenic potency of the HIV-1 polytope DNA vaccine containing CpG oligodeoxynucleotides (CpG-ODNs) in BALB/c mice. To this end, after verifying the expression of the recombinant sequence in the eukaryotic HEK 293 cell line, it was amplified and extracted in the prokaryotic host cells (E. coli DH5α)) and then formulated and administered intramuscularly (IM) to the experimental mice (on days 0, 14, and 28) with and without CpG-ODNs adjuvant. <br /><em><strong>Results:</strong></em> Taken together, the results demonstrated that CpG-ODNs adjuvanted DNA vaccine could significantly elicit cellular and humoral immune responses in the immunized animals in comparison with the control ones (p <0.05). <br /><em><strong>Conclusion:</strong></em> Regarding the obtained results and also considering the advantages of polytopic and DNA vaccines, this approach might be considered a new regimen in HIV-1/AIDS vaccination.
Cellular immunity CpG oligodeoxynucleotides DNA vaccine HIV,1 Humoral immunity
https://ijbms.mums.ac.ir/article_17608.html
https://ijbms.mums.ac.ir/article_17608_8dd3e975fe32b69057c700e98b50c4f1.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
3
2021
03
01
Effects of self-assembled cell-penetrating peptides and their nano-complexes on ABCB1 expression and activity
383
390
EN
Mehri
Niazi
0000-0001-8663-7990
Student Research Committee, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
niazimas1989@gmail.com
Parvin
Zakeri-Milani
0000-0003-3243-587X
Liver and Gastrointestinal Diseases Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
pzakeri@tbzmed.ac.ir
Mehdi
Soleymani-Goloujeh
0000-0002-6478-3951
Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
mehdiemperor@gmail.com
Ali
Mohammadi
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
ali_mohammadi6868@yahoo.com
Muhammad
Sarfraz
College of Pharmacy, Al Ain University of Science and Technology, Al Ain 64141, UAE
muhammad.sarfraz@aau.ac.ae
Raimar
Löbenberg
Faculty of Pharmacy and Pharmaceutical Sciences, Katz Group Centre for Pharmacy and Health Research, University of Alberta, Edmonton, Alberta, T6G 2H5, Canada
raimar@ualberta.ca
saeedeh
Najafi-Hajivar
Student Research Committee, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
saeede.hajivar@yahoo.com
Javid
Shahbazi Mojarrad
Biotechnology Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
shahbazi_j@tbzmed.ac.ir
Masoud
Farshbaf
Student Research Committee, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
masoud.farshbaf91@gmail.com
Hadi
Valizadeh
0000-0003-4157-6279
Drug Applied Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
valizadeh@tbzmed.ac.ir
10.22038/ijbms.2021.51675.11727
<em><strong>Objective(s):</strong></em> Doxorubicin (Dox) is one of the most well-known chemotherapeutics that are commonly applied for a wide range of cancer treatments. However, in most cases, efflux pumps like P-glycoprotein (P-gp), expel the taken drugs out of the cell and decrease the Dox bioavailability. Expression of P-gp is associated with elevated mRNA expression of the ATP-binding cassette B1 (ABCB1) gene. <br /><em><strong>Materials and Methods:</strong></em> In the current study, different sequences of cell-penetrating peptides (CPPs) containing tryptophan, lysine, and arginine and their nano-complexes were synthesized and their impact on the expression and activity of the ABCB1 gene was evaluated in the A549 lung carcinoma cell line. Furthermore, the cellular uptake of designed CPPs in the A549 cell line was assessed. <br /><em><strong>Results:</strong></em> The designed peptides, including [W4K4], [WR]3-QGR, R10, and K10 increased Dox cytotoxicity after 48 hr. Furthermore, arginine-rich peptides showed higher cellular uptake. Rhodamin123 accumulation studies illustrated that all the obtained peptides could successfully inhibit the P-gp pump. The designed peptides inhibited the ABCB1 gene expression, of which, [W4K4] resulted in the lowest expression ratio. <br /><em><strong>Conclusion:</strong></em> [W4K4], [WR]3-QGR, R10, and K10 could successfully increase the Dox cytotoxicity by decreasing the efflux pump gene expression.
Cancer therapy CPPs Doxorubicin Multi,drug resistance P,gp
https://ijbms.mums.ac.ir/article_17578.html
https://ijbms.mums.ac.ir/article_17578_a94f09204cb4fc9dc80f6983b3df3812.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
3
2021
03
01
Therapy with new generation of biodegradable and bioconjugate 3D printed artificial gastrointestinal lumen
391
399
EN
Matin
Karbasian
0000-0003-1589-7298
Department of Mechanical Engineering, Khomeinishahr Branch, Islamic Azad University, Khomeinishahr/Isfahan, Isfahan, Iran
matin_karbaasian21@yahoo.com
S. Ali
Eftekhari
Department of Mechanical Engineering, Khomeinishahr Branch, Islamic Azad University, Khomeinishahr/Isfahan, Isfahan, Iran
seyedalieftekhariiau@iaukhsh.ac.ir
Mohammad
Karimzadeh Kolamroudi
Mechanical Engineering Department, Eastern Mediterranean University, Gazimagusa, TRNC, Via Mersin 10 Turkey
mohammad.karimzadeh87@yahoo.com
Bahareh
Kamyab Moghadas
Department of Applied Researches, Chemical, Petroleum & Polymer Engineering Research Center, Shiraz Branch, Islamic Azad University, Shiraz, Iran
kamyab_bahareh@yahoo.com
Peiman
Nasri
Metabolic Liver Disease Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
peiman_nasrii@med.mui.ac.ir
Amir
Jasemi
Department of Chemical Engineering, Shiraz Branch, Islamic Azad University, Shiraz, Iran
jasemi_amir@siau.ac.ir
Mahshid
Telloo
Firoozgar Hospital, Iran University of Medical Sciences, Tehran, Iran
mahshid_tellow@mui.ac.ir
Saeed
Saber-Samandari
New Technologies Research Center, Amirkabir University of Technology, Tehran, Iran
saeed.ss@aut.ac.ir
Amirsalar
Khandan
0000-0001-8878-5233
New Technologies Research Center, Amirkabir University of Technology, Tehran, Iran
amir_salar_khandan@yahoo.com
10.22038/ijbms.2021.47925.11013
<em><strong>Objective(s):</strong></em> Many patients die due to vascular, gastrointestinal lumen problems, and coronary heart diseases. Synthetic vessels that are made of biodegradable-nanofiber polymers have significant properties such as proper biodegradability and efficient physical properties such as high strength and flexibility. Some of the best options for supporting cells in soft tissue engineering and design are applications of thermoplastic polyurethane polymer in the venous tissue. In this study, the first nanoparticle-reinforced polymeric artificial prosthesis was designed and tested to be used in the human body.<br /><em><strong>Materials and Methods:</strong></em> In this study, artificial gastrointestinal lumen were fabricated and prepared using a 3D printer. To improve cell adhesion, wettability properties and mechanical stability of elastin biopolymer with magnetic nanoparticles (MNPs) as well as single-walled carbon nanotubes (SWCNT) were prepared as separate filaments. MNPs were made in 5–7 mm sizes and then examined for mechanical, biological, and hyperthermia properties. Then, the obtained results of the gastrointestinal lumen were simulated using the Abaqus software package with a three-branch. The results were evaluated by X-ray diffraction (XRD) and scanning electron microscopy (SEM) for morphology and phase analysis. <br /><em><strong>Results:</strong></em> The obtained results of the designed vessels showed remarkable improvement in mechanical properties of the SWCNT vessels and hyperthermia properties of the vessels containing the MNPs. The results of computational fluid dynamics (CFD) analysis showed that the artificial vessels had lower shear stress at the output. <br /><em><strong>Conclusion:</strong></em> Five-mm MNP containing vessels showed noticeable chemical and biological properties along with ideal magnetic results in the treatment of thrombosis and vascular obstruction.
Cardiovascular,Gastrointestinal lumen,Magnetite nanoparticle,Polyurethane,tissue
https://ijbms.mums.ac.ir/article_17609.html
https://ijbms.mums.ac.ir/article_17609_be1a80ef7b6e2af78ec11456ce3aff71.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
3
2021
03
01
Correlation between antibiotic resistance and phylogenetic types among multidrug-resistant Escherichia coli isolated from urinary tract infections
400
407
EN
Humera
Nazir
0000-0003-1486-3803
Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan
humeranazir27@gmail.com
Mubashar
Aziz
0000-0002-7461-1272
Institute of Pure and Applied Biology, (Microbiology division) Bahauddin Zakariya University, Multan 60900, Pakistan
mubashirazizsheikh@gmail.com
Zulfiqar
Ali
Mirani
0000-0002-3416-6311
Department of Microbiology, PCSIR Laboratories Complex, Karachi 74000, Pakistan
mirani_mrsa@yahoo.com
Ahsan
Sattar
Sheikh
Institute of Molecular Biology & Biotechnology, University of Lahore, Lahore, Pakistan
ahsanssheikh@gmail.com
Muhammad
Qamar
Saeed
Institute of Pure and Applied Biology, (Microbiology division) Bahauddin Zakariya University, Multan 60900, Pakistan
mqamarsaeed@bzu.edu.pk
Masroor
Hussain
Department of Biotechnology, University of Science & Technology Bannu, Pakistan.
masroorktk@gmail.com
Aleem
Ahmed
Khan
Institute of Pure and Applied Biology, (Microbiology division) Bahauddin Zakariya University, Multan 60900, Pakistan
aleemahmad@bzu.edu.pk
Tahira
Ruby
Department of Zoology, The Islamia University of Bbahawalpur,61300, Bahawalpur, Pakistan
tahira.ruby@iub.edu.pk
Naseem
Rauf
PCSIR Laboratories Islamabad, Pakistan
naseemrauf@yahoo.com
10.22038/ijbms.2021.47095.10865
<em><strong>Objective(s):</strong></em> Emergence of multidrug resistance has reduced the choice of antimicrobial regimens for UTIs. To understand the association of phenotype and genotype among uropathogens.<br /><em><strong>Materials and Methods:</strong></em> Six hundred and twenty-eight (628) urine samples were collected and analyzed. Antibiotic sensitivity pattern was determined by the Kirby-Bauer Disc Diffusion Method and minimum inhibitory concentration (MIC) was tested by the E test. Fluoroquinolone resistant mutations in QRDR of gyrA and ParC, phylogenetic groups, and PAIusp subtype were detected by PCR.<br /><em><strong>Results:</strong></em> Most prevalent uropathogens were Escherichia coli (53.2%) followed by Klebsiella pneumoniae (21%). Multidrug- resistance was observed in > 50% cases for third-generation cephalosporins and ciprofloxacin and lowest in meropenem. E. coli (66.2%) and K. pneumonia (64.4%) were extended-spectrum β-lactamases (ESBLs) producers. MIC to trimethoprim-sulfamethoxazole was highest in E. coli (>1024 µg/ml). In 80 (24%) of the 334 E. coli isolates analyzed in detail, 54 fluoroquinolones (FQ) resistant isolates carried mutations (S83L, D87N, S80I, E84V) in QRDR of gyrA and ParC. Out of 54 FQ-resistant isolates, 43 (79.6%) isolates belonged to the phylogenetic group B2, and 11(20.4%) belonged to group D. Isolates belonged to group B2, 38 (88.4%) of the 43 isolates carried PAIusp subtype IIa and high frequency of mutation E84V in ParC was detected in 37 (97.4%). Other mutations, such as S80I, S83L in gyrA and D87N in ParC were found in all resistant isolates.<br /><em><strong>Conclusion:</strong></em> Correlations between phenotype and genotype provided a basis to understand the resistance development in uropathogens, and PAIusp subtyping indicated that E. coli belonged to the B2 group.
Escherichia coli,ESBL,MDR,PCR,UTI
https://ijbms.mums.ac.ir/article_17610.html
https://ijbms.mums.ac.ir/article_17610_a8c02d7ed47723482ea5e0a4ca8953fc.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
3
2021
03
01
Association of NOS3-c.894G>T transversion with susceptibility to metabolic syndrome in Azar-cohort population: A case-control study and in silico analysis of the SNP molecular effects
408
419
EN
Ensiyeh
Seyedrezazadeh
0000-0001-5783-5218
Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
e.s.rezazadeh@tbzmed.ac.ir
Elnaz
Faramarzi
Liver and Gastrointestinal Diseases Research Center, Clinical Research Institute, Tabriz University of Medical Sciences, Tabriz, Iran
elnazfaramarzi849@gmail.com
Nasim
Bakhtiyari
Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
bakhtiyari.nasim1@gmail.com
Atefeh
Ansarin
Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
atefeh.ansarin@gmail.com
Neda
Gilani
Department of Statistics and Epidemiology, Faculty of Health, Tabriz University of Medical Sciences, Tabriz, Iran
neda.gilani@gmail.com
Amir
Amiri-Sadeghan
Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
amiri.sadeghan.a@gmail.com
Maryam
Seyyedi
Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
seyyedim@tbzmed.ac.ir
Khalil
Ansarin
Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
dr.ansarin@gmail.com
Younes
Aftabi
0000-0002-8692-8867
Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
aftabiy@tbzmed.ac.ir
10.22038/ijbms.2021.50528.11511
<em><strong>Objective(s):</strong></em> We investigated whether NOS3-c.894G>T transversion (rs1799983), which causes the substitution of glutamate with aspartate (E298D) in the oxygenase domain of endothelial nitric oxide synthase (eNOS), is associated with susceptibility to metabolic syndrome (MetS) risk in Iranian-Azerbaijanis. <br /><em><strong>Materials and Methods:</strong></em> The frequencies of the alleles and genotypes were compared in the 300 cases and 300 controls using PCR-RFLP assay. Also, higher-order MetS interaction with the genotypes, gender, age, and body mass index (BMI) was evaluated by classification and regression tree (CART) analysis. In silico analysis was done to introduce a hypothesis describing the molecular effects of NOS3-c.894G>T. <br /><em><strong>Results:</strong></em> The T allele (OR:1.46; CI:1.054-2.04; P=0.02), GT genotype (OR:1.44; CI:1.02-2.03; P=0.03), and dominant model (TT+GT vs GG, OR:1.48; CI:1.06-2.06; P=0.01) were found to be associated with increased risk of MetS. In the male subpopulation TT genotype (OR:7.19; CI:1.53-33.70; P=0.01) was discovered to be associated with increased odds of MetS. CART analysis showed that NOS3-c.894G>T genotypes and BMI significantly contribute to modulating MetS risk. Furthermore, in silico investigation revealed that c.894G>T may alter eNOS function through affecting interactions of its oxygenase domain with proteins such as B2R, b-actin, CALM1, CAV1, GIT1, HSP90AA1, NOSIP, and NOSTRIN. <br /><em><strong>Conclusion:</strong></em> We showed that NOS3-c.894G>T was associated with an increased risk of MetS in Iranian-Azerbaijanis, and BMI modulates the effects of NOS3-c.894G>T genotypes on MetS risk. Also, in silico analysis found that NOS3-c.894G>T may affect the interaction of the eNOS oxygenase domain with its several functional partners.
Azar,cohort Bioinformatics Metabolic syndrome Nitric oxide pathway rs1799983
https://ijbms.mums.ac.ir/article_17603.html
https://ijbms.mums.ac.ir/article_17603_5c38c77c124485314f876bbf5fa21e79.pdf