Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
4
2021
04
01
Effect of eggplant (Solanum melongena) on the metabolic syndrome: A review
420
427
EN
Fatemeh
Yarmohammadi
0000-0002-0552-8766
Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran
yarmohammadif971@mums.ac.ir
Mahboobeh
Ghasemzadeh Rahbardar
https://orcid.org/00
Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
ghasemzadeh_mahboobeh@yahoo.com
Hossein
Hosseinzadeh
0000-0002-3483-851X
Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
hosseinzadehh@mums.ac.ir
10.22038/ijbms.2021.50276.11452
Metabolic syndrome (MetS), also known as syndrome X, is a significant risk factor for cardiovascular disease incidence and mortality. Increasing age, obesity, physical inactivity, smoking, and positive family history are the risk factors associated with MetS, which increases the risk of diabetes, cardiovascular disease, hypertension, hyperlipidemia, and obesity. Chemical compounds in the treatment of metabolic complications are associated with a lack of efficacy and severe side effects. Numerous studies have described the importance of herbs and natural products to treat human diseases. Therefore, nowadays, herbs-based diets and herbal medicines are recommended for the management of various diseases. The protective effects of several herbs have been reported against MetS such as rosemary, avocado, and silymarin. Eggplant (Solanum melongena) is a rich source of phenolic and alkaloid compounds. It possesses various pharmacological effects, including, anti-oxidant, antidiabetic, antihypertensive, and antihyperlipidemic, which has been supported by numerous investigations. In this review, we evaluated the effects of eggplant on MetS and its complications comprising diabetes, high blood pressure, hyperlipidemia, and obesity. <br />According to these studies, eggplant can control diabetes through the anti-oxidative properties and inhibition of α-amylase and α-glucosidase activity. Also, eggplant has exerted an antihypertensive effect via ACE inhibitory activity. Eggplant may have shown protective effects on hyperlipidemia and obesity via the induction of lipoprotein lipase activity and the reduction of pancreatic lipase activity.Eggplant can be useful in the treatment of MetS and its complications.
Antihypertensive,Antihyperlipidemic,Aubergine,Diabetes,Eggplant,metabolic syndrome,Solanum melongena
https://ijbms.mums.ac.ir/article_17743.html
https://ijbms.mums.ac.ir/article_17743_73b548e00779bc656e273d38c7211c9d.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
4
2021
04
01
An updated systematic review and meta-analysis on Mycobacterium tuberculosis antibiotic resistance in Iran (2013-2020)
428
436
EN
Farzad
Khademi
0000-0001-6181-4903
Department of Microbiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
k_farzad@yahoo.com
Amirhossein
Sahebkar
0000-0002-8656-1444
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
amir_saheb2000@yahoo.com
10.22038/ijbms.2021.48628.11161
This updated systematic review and meta-analysis follows two aims: 1) to assess Mycobacterium tuberculosis (M. tuberculosis) antibiotic resistance in Iran from 2013 to 2020 and, 2) to assess the trend of resistance from 1999 to 2020. Several national and international databases were systematically searched through MeSH extracted keywords to identify 41 published studies addressing drug-resistant M. tuberculosis in Iran. Meta-analysis was done based on the PRISMA protocols using Comprehensive Meta-Analysis software. The average prevalence of resistance to first- and second-line anti-TB drugs, multidrug-resistant TB (MDR-TB) and extensively drug-resistant TB (XDR-TB) in new and previously treated tuberculosis (TB) cases in Iran during 2013–2020 were as follows: isoniazid 6.9%, rifampin 7.9%, ethambutol 5.7%, pyrazinamide 20.4%, para-aminosalicylic acid 4.6%, capreomycin 1.7%, cycloserine 1.8%, ethionamide 11.3%, ofloxacin 1.5%, kanamycin 3.8%, amikacin 2.2%, MDR-TB 6.3% and XDR-TB 0.9%. Based on the presented data, M. tuberculosis resistance to first- and second-line anti-TB drugs, as well as MDR-TB, was low during 2013–2020 in Iran. Furthermore, there was a declining trend in TB drug resistance from 1999 to 2020. Hence, to maintain the current decreasing trend and to control and eliminate TB infection in Iran, continuous monitoring of resistance patterns is recommended.
Antibiotic Iran Meta,analysis Mycobacterium tuberculosis Resistance
https://ijbms.mums.ac.ir/article_17753.html
https://ijbms.mums.ac.ir/article_17753_49d865e670edd3a4a30a726343e1a55f.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
4
2021
04
01
Subtyping β -lactamase-producing Escherichia coli strains isolated from patients with UTI by MLVA and PFGE methods
437
443
EN
Alireza
Dolatyar Dehkharghani
0000-0003-2370-3668
Department of Microbiology, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
ardltyr@ymail.com
Setareh
Haghighat
0000-0002-6605-5245
Department of Microbiology, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
setareh_haghighat@yahoo.com
Marjan
Rahnamaye-Farzami
0000-0001-9745-1657
Department of Microbiology, Research Center of Reference Health Laboratory, Ministry of Health and Medical Education, Tehran, Iran
marjan.farzami@gmail.com
Masoumeh
Douraghi
Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
douraghim@yahoo.com
Mohammad
Rahbar
0000-0001-8419-3791
Department of Microbiology, Research Center of Reference Health Laboratory, Ministry of Health and Medical Education, Tehran, Iran
rahbar.reflab@gmail.com
10.22038/ijbms.2021.49790.11372
<em><strong>Objective(s):</strong></em> Strain subtyping is an important epidemiological tool to trace contamination, determine clonal relationships between different strains, and the cause of outbreaks. Current subtyping methods, however, yield less than optimal subtype discrimination. Pulsed-field gel electrophoresis is the gold standard method for Escherichia coli and Multiple-Locus Variable-number tandem repeat Analysis is a rapid PCR-based method. The purpose of this study was to evaluate MLVA and PFGE methods for subtyping β -lactamase-producing E. coli strains isolated from urinary tract infections.<br /><em><strong>Materials and Methods:</strong></em> Overall, 230 E. coli isolates from patients with urinary tract infections were examined for antimicrobial susceptibility testing. 10-loci and 7-loci MLVA and PFGE methods were used for molecular typing of β -lactamase-producing E. coli isolates. <br /><em><strong>Results:</strong></em> Out of 230 isolates, 130 (56.5%) β -lactamase-producing E. coli isolates were found in this study. The diversity indices of the VNTR loci showed an average diversity of 0.48 and 0.54 for 7-loci and 10-loci MLVA, respectively. The discriminatory power of PFGE showed a value of 0.87. The discordance between the methods was high. <br /><em><strong>Conclusion:</strong></em> Our study showed that PFGE is more discriminatory than MVLA. MLVA is a PCR- based method and can generate unmistakable data, in contrast to PFGE. Optimization of polymorphic VNTR is essential to improve the discriminatory power of MLVA based on geographical region.
Beta,lactamase Escherichia coli Molecular typing Pulsed,field gel electrophoresis VNTR
https://ijbms.mums.ac.ir/article_17756.html
https://ijbms.mums.ac.ir/article_17756_aa81eeea70129a50b505db72ad1c2685.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
4
2021
04
01
Non-collagenous extracellular matrix protein dermatopontin may play a role as another component of transforming growth factor-β signaling pathway in colon carcinogenesis
444
450
EN
Ariane
Sadr-Nabavi
0000-0003-3162-8792
Medical Genetic Research Center (MGRC), School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
sadrnabavia@mums.ac.ir
Samaneh
Bouromand-Noughabi
Department of Pathology, Faculty of Medicine, Mashhad, University of Medical Sciences, Mashhad, Iran
samaneh.boroumand@gmail.com
Naser
Tayebi-Meybodi
Department of Pathology, Faculty of Medicine, Mashhad, University of Medical Sciences, Mashhad, Iran
tayebimeybodi@yahoo.com
Kimia
Dadkhah
Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran
kimiadadkhah@yahoo.com
Nafiseh
Amini
Medical Genetic Research Center (MGRC), School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
nafisehamini@yahoo.com
Alfons
Meindl
Frauenklinik der Technischen Universität München, Klinikum rechts der Isar, München, Germany
alfonsmeindl@yahoo.com
Mohammad Reza
Abbaszadegan
0000-0003-4539-048X
Medical Genetic Research Center (MGRC), School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
abbaszadeganmr@mums.ac.ir
10.22038/ijbms.2021.46422.10720
<em><strong>Objective(s):</strong></em> Dermatopontin (DPT) is an extracellular matrix protein that plays roles in increasing the activity of transforming growth factor-β (TGF-β) and induction of cell quiescence. These roles suggest a tumor suppressor function for DPT. This study aimed to investigate changes in DPT gene expression in colorectal cancer providing a better understanding of its carcinogenesis.<br /><em><strong>Materials and Methods:</strong></em> We used Matched Tumor/Normal Expression Array and Cancer Profiling Arrays I containing 34 and 7 cases of colorectal cancer and their matched controls, respectively, to test DPT expression. In addition, 38 newly diagnosed cases of colorectal cancer were enrolled and their fresh colonic tumoral and normal specimens were obtained. DPT mRNA expression was analyzed using real-time PCR. In cases with DPT under expression, exonic regions of the DPT gene were sequenced using the Sanger method.<br /><em><strong>Results:</strong></em> In array samples, DPT expression was decreased in 82.9% (34/41), increased in 12.2% (5/41), and had no changes in 4.9% (2/41). DPT was decreased in 14 fresh samples (36.8%), while 12 cases (31.6%) showed overexpression and the others had no changes. DPT expression showed no significant difference among various tumor grades and stages. The frequencies of DPT overexpression were higher in tumors having lymph node involvement (47.7% vs 28%, P=0.59). In 2 cases mutations were detected that may be responsible for decreased expression of DPT.<br /><em><strong>Conclusion:</strong></em> The similarities between changing patterns of DPT and TGF-β expression in colorectal cancer demonstrate that DPT may act as a pre-receptor component of the TGF-β signaling pathway in colon carcinogenesis.
Colorectal cancer Dermatopontin Real,Time PCR Sanger sequencing TGF,β
https://ijbms.mums.ac.ir/article_17758.html
https://ijbms.mums.ac.ir/article_17758_2e5b422946c2b7c06b9419a479da3522.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
4
2021
04
01
The therapeutic effects of berberine plus sitagliptin in a rat model of fatty liver disease
451
459
EN
Soraya
Mehrdoost
Department of Biology, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
soraya_mehrdoost@yahoo.com
Parichehreh
Yaghmaei
0000000158996777
Department of Biology, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
yaghmaei_p@srbiau.ac.ir
Hanieh
Jafary
0000-0002-0958-9072
Department of Biology, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
h-jafary@srbiau.ac.ir
Azadeh
Ebrahim-Habibi
0000-0002-8993-4859
Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
aehabibi@sina.tums.ac.ir
10.22038/ijbms.2021.52239.11822
<em><strong>Objective(s):</strong></em> Fatty liver disease (FLD) is a disorder related to accumulation of excess fat within the hepatocytes. In this study, the effects of Berberine, a natural compound, and Sitagliptin as a DPP-4 inhibitor, were observed in a rat model of FLD.<br /><em><strong>Materials and Methods:</strong></em> Forty male rats were divided into five groups (n=6) including the control group (normal food and water), high-fat group (high-fat diet (HF) for 6 weeks), Berberine group (HF with oral administration of Berberine at 150 mg/kg for 6 weeks), Sitagliptin group (HF with oral administration of Sitagliptin at 10 mg/kg for 6 weeks), and Berberine/ Sitagliptin group (HF diet within combination with oral administration of Berberine 75 mg/kg and Sitagliptin 5 mg/kg for 6 weeks). Animals were examined for weight gain, serum and hepatic biochemical parameters, tissue histology, expression of glucose transporter type 4 (GLUT4) mRNA, and protein expression of Adiponectin receptor2 (AdipoR2) and extracellular signal-regulated kinase (ERK) and phoERK.<br /><em><strong>Results:</strong></em> The results showed that ALT, AST, lipid profile, insulin, glucose, MDA, and TNF-α were significantly improved in high-fat rats treated with Berberine/ Sitagliptin compared with HF and Sitagliptin, and Berberine alone groups. SOD and adiponectin levels in Berberine/ Sitagliptin group were also significantly increased compared with the other groups. Immunoblot analysis showed that the expression of pho-ERK/ERK was significantly decreased and expression of AdipoR2 significantly increased in the Berberine/ Sitagliptin group compared with other groups.<br /><em><strong>Conclusion:</strong></em> Co-administration of Berberine and Sitagliptin is an effective therapeutic regimen for conditions associated with hyperlipidemia.
Adiponectin receptor2 DDP,4 Glucose ttransporter type 4 Natural compound Non,alcoholic fatty liver disease Pho,ERK/ERK
https://ijbms.mums.ac.ir/article_17754.html
https://ijbms.mums.ac.ir/article_17754_f7e7bc87a739546746ecc8f4603b49f4.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
4
2021
04
01
Voltage-gated potassium channels are involved in oxymatrine-regulated islet function in rat islet β cells and INS-1 cells
460
468
EN
Jingying
Gao
0000-0002-3263-2040
Department of Pediatrics, Shanxi Medical University, Taiyuan, China
13633513158@163.com
Lixia
xia
Department of Pediatrics, Shanxi Medical University, Taiyuan, China
xlxmedicine@163.com
Yuanyuan
Wei
Department of Pediatrics, Shanxi Medical University, Taiyuan, China
weiyuanyuanerke@163.com
10.22038/ijbms.2021.52449.11850
<em><strong>Objective(s):</strong></em> Oxymatrine can regulate glucose metabolism. But the underlying mechanisms remain unclear. We investigated the relationship of oxymatrine and voltage-gated potassium (Kv) channel in rat islet β cells and INS-1 cells.<br /><em><strong>Materials and Methods:</strong></em> Insulin secretion and Kv channel currents were tested by radioimmunoassay and patch-clamp technique, respectively. The INS-1 cell viability was detected using cell counting kit-8 experiments. Flowcytometry analysis and western blot were employed for cell apoptosis and protein levels, respectively. INS-1 cell proliferation was assessed by the 5-Ethynyl-2’- deoxyuridine method. <br /><em><strong>Results:</strong></em> Oxymatrine potentiated insulin secretion at high glucose (p <em><strong>Conclusion:</strong></em> The results indicate that oxymatrine can stimulate insulin secretion and decrease kv channel currents in islet β cells. Besides, oxymatrine also increases cell viability, proliferation, and reduces cell apoptosis in INS-1 cells. The effects of oxymatrine are related to kv channels. This finding provides new insight into the mechanisms of oxymatrine-regulated islet function.
Apoptosis Diabetes mellitus Insulin secretion Oxymatrine Potassium channel Voltage,gated
https://ijbms.mums.ac.ir/article_17755.html
https://ijbms.mums.ac.ir/article_17755_742e709253b90b76eb5cae310ff59e81.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
4
2021
04
01
Effects of morphine and NeuroAid on the expression levels of GluN2A and GluN3A in the hippocampus and striatum of rats
469
475
EN
Katayoun
Heshmatzad
0000-0001-9263-0744
Cognitive and Neuroscience Research Center (CNRC), Amir-Almomenin Hospital, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
heshmatzadk@yahoo.com
Mohammad
Nasehi
3592-1981-0002-0000
Cognitive and Neuroscience Research Center (CNRC), Amir-Almomenin Hospital, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
mo58na@yahoo.com
Salar
Vaseghi
0000-0002-2436-9887
Cognitive and Neuroscience Research Center (CNRC), Amir-Almomenin Hospital, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
salarv67@yahoo.com
10.22038/ijbms.2021.52004.11787
<em><strong>Objective(s):</strong></em> NMDA glutamatergic receptors are heteromeric receptors with various subunits. GluN2A and GluN3A subunits specify the functional heterogeneity of NMDA receptors. These subunits play a key role in the induction of LTP and synaptic plasticity. Note that, the function of NMDA subunits has interaction with the mechanism of morphine. On the other hand, NeuroAid is a Chinese traditional medicine with neuroprotective and anti-apoptotic effects. In this study, we aimed to investigate the effect of morphine and NeuroAid on expression levels of GluN2A and GluN3A in the hippocampus and striatum of rats. <br /><em><strong>Materials and Methods:</strong> </em>Morphine sulfate (increasing doses) and NeuroAid (2.5 mg/kg) were injected intraperitoneally. Real-time PCR was used to assess gene expression.<br /><em><strong>Results:</strong></em> The results showed that morphine increased the expression of GluN2A in the hippocampus and striatum, while NeuroAid increased the expression of both genes in the hippocampus and decreased the expression of GluN3A in the striatum. NeuroAid increased the expression of GluN3A in the hippocampus and GluN2A in the striatum of morphine-addicted rats. <br /><em><strong>Conclusion:</strong></em> NeuroAid may have interaction with the effect of morphine on glutamatergic neurotransmission; however, this study is innovative and novel, thus, further studies are needed to better understand the effect of NeuroAid and morphine on hippocampal and striatal glutamatergic neurotransmission.
Glutamate,Hippocampus,Morphine,NeuroAid,Striatum
https://ijbms.mums.ac.ir/article_17736.html
https://ijbms.mums.ac.ir/article_17736_8ebadef449deed33ae63dcad7e67cf54.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
4
2021
04
01
Immunization against Pseudomonas aeruginosa using Alg-PLGA nano-vaccine
476
482
EN
Saeid
Azimi
0000-0001-6791-1755
Parseh Institute of Iran, Tehran, Iran
saeidazimi68@yahoo.com
Leila
Safari Zanjani
0000-0003-4183-5509
Department of Cellular and Molecular Biology, Zanjan Branch, Payame Noor of Zanjan, Zanjan, Iran
leila_safari_z2007@yahoo.com
10.22038/ijbms.2021.52217.11813
<em><strong>Objective(s):</strong></em> Pseudomonas aeruginosa is the bacterium that causes of pulmonary infection among chronically hospitalized patients. Alginate is a common surface antigen of P. aeruginosa with a constant structure that which makes it an appropriate target for vaccines. In this study, P. aeruginosa alginate was conjugated with to PLGA nanoparticles, and its immunogenicity was characterized as a vaccine.<br /><em><strong>Materials and Methods:</strong></em> Alginate was isolated from a mucoid strain of P. aeruginosa and conjugated with to PLGA with˝ N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride ˝= ˝EDAC˝ and N-Hydroxysuccinimide (NHS). Chemical characterization of prepared nano-vaccine was performed using FTIR Spectroscopy, Zetasizer, and Atomic Force Microscopy (AFM). The immunogenicity of this nano-vaccine was evaluated through intramuscular injection into BALB/c mice. Four groups of mice were subjected to the injection of alginate–PLGA, and two weeks after the last administration step, opsonophagocytosis assay, IgG detection, challenge, and cytokine determination via ELISA were carried out.<br /><em><strong>Results:</strong></em> Alginate-PLGA conjugation was corroborated by FTIR, Zetasizer, and AFM. The ELISA consequence showed that alginate was prospering in the instigation of the humoral immunity.The immunogenicity enhanced against the alginate-PLGA. Remarkably diminished bacterial titer in the spleen of the immunized mice posterior to challenge with PAO1 strain in comparison with the alginate alone and control groups.<br /><em><strong>Conclusion:</strong></em> The bacterial burden in the spleen significantly decreased after the challenge (p <0.05). The opsonic activity was significantly increased in the alginate- PLGA group (p <0.05).
Alginate,Cytokine,Opsonophagocytosis,PLGA,Pseudomonas aeruginosa
https://ijbms.mums.ac.ir/article_17745.html
https://ijbms.mums.ac.ir/article_17745_710125b2fc602e7c597ab16a38961f17.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
4
2021
04
01
NLRP3-inflammasome activation is associated with epithelial-mesenchymal transition and progression of colorectal cancer
483
492
EN
Yasser
Marandi
0000-0001-7241-2408
Department of Clinical Biochemistry, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
yasser.marandi59@gmail.com
Shahriar
Hashemzadeh
Department of General Surgery, Imam Reza Hospital, Tabriz University of Medical Sciences, Tabriz, Iran
shahriar_90@yahoo.com
Heidar
Tayebinia
0000-0002-7243-660X
Department of Clinical Biochemistry, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
tavilani@gmail.com
Jamshid
Karimi
0000-0001-8253-7124
Department of Clinical Biochemistry, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
jamshidkarimi2013@gmail.com
Alireza
Zamani
Department of Immunology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
a_zamani@umsha.ac.ir
Iraj
Khodadadi
0000000190484528
Department of Clinical Biochemistry, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Research Center for Nutrition Health, Hamadan University of Medical Sciences, Hamadan, Iran
khodadadi@umsha.ac.ir
10.22038/ijbms.2021.52355.11835
<em><strong>Objective(s):</strong></em> Since activation of NLRP3 inflammasome results in the production of interleukin-1β (IL 1β) and initiation of inflammation as the key players in development of cancer, this study investigated possible activation of NLRP3 inflammasome during the progression of colorectal cancer (CRC) and evaluated the role of NLRP3 inflammasome in epithelial-mesenchymal transition (EMT) process. <br /><em><strong>Materials and Methods:</strong></em> Tissue samples were collected from cancerous (test) and adjacent normal tissues (control) of forty-three male CRC patients (18 grade I and 25 grade III). The gene expression and protein levels were determined by qRT PCR and Western blotting, respectively, and tissue morphological was examined by histopathology. <br /><em><strong>Results:</strong> </em>The gene and protein expression levels of transforming growth factor-β (TGF β), IL 1β, nuclear factor κB (NF κB), NLRP3, and caspase-1, as well as the enzyme activity of caspase-1, were significantly increased in CRC. mRNA and protein levels of TGF-β, mature IL 1β, NF κB, and NLRP3 were higher in patients with grade III. EMT markers N cadherin, vimentin, and MMP 9 markedly increased in CRC, and were higher in grade III than grade I, whereas expression of E-cadherin declined by the progression of CRC. NLRP3 protein level was inversely correlated with E-cadherin whereas it positively was correlated with IL 1β, active NF κB, N cadherin, vimentin, and MMP 9. <br /><em><strong>Conclusion:</strong> </em>This study for the first time showed that activation of NLRP3 inflammasome contributed to the progression of CRC and is correlated with the EMT process. Although the present study showed that EMT markers are positively correlated with tumor grade, further investigations are required to strongly link the EMT markers to the progression of CRC.
Colorectal neoplasms Epithelial,mesenchymal transition Inflammasome NLRP3 Transforming growth factor,β
https://ijbms.mums.ac.ir/article_17735.html
https://ijbms.mums.ac.ir/article_17735_15386d75d2eabedbb4d193a229f5f228.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
4
2021
04
01
Kaempferide improves oxidative stress and inflammation by inhibiting the TLR4/IκBα/NF-κB pathway in obese mice
493
498
EN
Heng
Tang
Department of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, PR China
1355956791th@sina.com
Qingfu
Zeng
Department of vascular surgery, The Second Affiliated Hospital of Nanchang University
260651407@qq.com
Nina
Ren
Guangdong Online Hospital Clinic, Guangdong Second Provincial People’s Hospital, Guangzhou 510317, PR China
1355956791@qq.com
Yunjie
Wei
Taihe Hospital Shiyan, Hubei, PR China
Quan
He
Department of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, PR China
851557800@qq.com
Ming
Chen
Department of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, PR China
chenmingcq77@163.com
Peng
Pu
pp841103@sina.com
Department of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, PR China
pp841103@sina.com
10.22038/ijbms.2021.52690.11892
<strong>Objective(s):</strong> Kaempferide (Ka), a major natural active component of Tagetes erecta L, has numerous pharmacological effects such as anti-obesity, anticancer, and anti-hypertension. However, there is no clear evidence that Ka is directly related to inflammation and oxidative stress in obese mice. We aimed to explore the effects of Ka on inflammation and oxidative stress and its mechanism.<br /><em><strong>Materials and Methods:</strong> </em>The obese mice were induced by a high-fat diet (HFD). The anti-obesity effect was tested by liver and body weight, liver and adiposity index, and white adipose tissue. Blood sample analysis was used to detect the hypolipidemic and hypoglycemic effects. The anti-oxidation effect was assessed using GSH, SOD, MDA, CAT, T-AOC, and other indicators. The anti-inflammatory effect was assessed using TNF-α, MCP-1, and Adiponectin. Western blot and Real-Time PCR were used to evaluate the related signaling pathways.<br /><em><strong>Results:</strong></em> Obesity, glycolipid metabolism disorder, inflammation, and oxidative stress developed in HFD mice. These changes can be effectively alleviated by Ka treatment for 16 weeks. Further studies have suggested that these beneficial effects of Ka may be associated with inhibition of the TLR4/IκBα/NF-κB signaling pathways. <br /><em><strong>Conclusion:</strong></em> Ka possesses important anti-obesity, hypoglycemic, and hypolipidemic effects. The mechanism may be causally associated with the TLR4/IκBα/NF-κB signaling pathway, which improves inflammation and oxidative stress.
Anti,inflammatory Anti,oxidation Kaempferide Obesity TLR4
https://ijbms.mums.ac.ir/article_17744.html
https://ijbms.mums.ac.ir/article_17744_d8277b2de3faa8d72fbce6f30bf69d66.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
4
2021
04
01
Pathogenic role of the SP/ NK1R system in GBM cells through inhibiting the thioredoxin system
499
505
EN
Fatemeh
Ghahremani
Department of Biology, Faculty of Science, Hakim Sabzevari University, Sabzevar, Iran
fatemeghahremani17222@gmail.com
Reihaneh
Sabbaghzade
Department of Biology, Faculty of Science, Hakim Sabzevari University, Sabzevar, Iran
r.sabbaghzadeh@hsu.ac.ir
Safieh
Ebrahimi
Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
ebrahimis961@mums.ac.ir
Hosein
Javid
Medical Laboratory Sciences Department, Varastegan Institute for Medical Sciences, Mashhad, Iran
javidh@varastegan.ac.ir
Javad
Ghahremani
Department of Medicine, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
bayegani@gmail.com
Seyed Isaac
Hashemy
0000-0002-1323-5250
Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
hashemyi@mums.ac.ir
10.22038/ijbms.2021.52902.11945
<em><strong>Objective(s):</strong></em> Glioblastoma multiforme (GBM), a highly aggressive Grade IV brain tumor, is a significant public health issue due to its poor prognosis and incurability. Neuropeptide substance P (SP) plays a critical role in GBM tumor growth and development via activation of neurokinin‐1receptor (NK1R). Moreover, SP is a pro-oxidant factor contributing to oxidative stress in various cell types. However, the link between SP and oxidative stress in cancer cells is not fully investigated. Here, we aimed to identify the effects of SP and NK1R antagonist, aprepitant, on the redox status of GBM cells.<br /><em><strong>Materials and Methods:</strong></em> Resazurin assay was employed to determine the effect of aprepitant on viability of U87 glioblastoma cells. 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) assay was employed to measure the levels of intracellular reactive oxygen species (ROS). A quantitative real-time polymerase chain reaction was applied to measure the expression of proteins of the thioredoxin system. Commercial kits (ZellBio GmbH) were also used to measure the enzymatic activity of these proteins.<br /><strong><em>Results:</em></strong> We found that SP increased ROS level in U87 GBM cells, and aprepitant significantly reduced this effect. Furthermore, we found that SP could also affect the thioredoxin system, a central antioxidant enzyme defense system. SP reduced both expression and enzymatic activity of the thioredoxin system’s proteins, Trx and thioredoxin reductase (TrxR) and these effects were significantly reduced by aprepitant. <br /><em><strong>Conclusion:</strong></em> Our results indicated that SP activation of NK1R represented a link between oxidative stress and GBM and highlighted the need for further validations in future studies.
Glioblastoma multiforme,Neurokinin 1 receptor,Oxidative stress,Substance P,Thioredoxin
https://ijbms.mums.ac.ir/article_17739.html
https://ijbms.mums.ac.ir/article_17739_68191cfb154f53cef0ea4e1bf37025d2.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
4
2021
04
01
Identification of the effects of acid-resistant Lactobacillus caseimetallopeptidase gene under colon-specific promoter on the colorectal and breast cancer cell lines
506
513
EN
Narges
Dadfarma
0000-0002-1577-8435
Department of Microbiology, Faculty of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran
n.dadfarma@yahoo.com
Jamileh
Nowroozi
0000-0003-4735-9208
Department of Microbiology, Faculty of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran
nowroozij@yahoo.com
Bahram
Kazemi
0000-0002-3072-8831
Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
kazemi@sbmu.ac.ir
Mojgan
Bandehpour
0000-0002-5479-2846
Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
bandehpour@gmail.com
10.22038/ijbms.2021.53015.11950
<em><strong>Objective(s):</strong></em> Anti-tumor effects of Lactobacilli as normal flora have been described. In a previous study, we identified a protein isolated from the bacterium Lactobacillus casei ATCC 39392 in acidic pH conditions named metallopeptidase. Therefore, we decided to evaluate the effect of the recombinant plasmid coding metallopeptidase protein on the inhibition, proliferation, or apoptosis of the colorectal and breast cancer cell lines. <br /><em><strong>Materials and Methods:</strong></em> Identified metallopeptidase gene of L. casei under the specific colon cancer promoter was transferred to the Human SW480 and MDA-MB231 cells. Cell viability was evaluated in these two cancer cell lines via MTT assay, apoptotic changes, and expression level of p53 and MAP2K1 genes in comparison with healthy blood cells as a control group. <br /><em><strong>Results:</strong></em> Viability of SW480 and MDA-MB231 cells was identified at 25% and 7%, respectively. An increase in apoptotic cell death in the SW480 cell line was observed as revealed by Tunnel staining. The expression assay of TP53 and MAP2K1 genes showed that MPL protein altered gene expression in a cell type-specific manner. Tunnel analyses showed that the pronounced cytotoxic effect of pEGFP-C2/MPL plasmid on SW480 cells was mediated through apoptosis. <br /><em><strong>Conclusion:</strong></em> These results suggest that endogenous recombinant MPL under colon specific promoter inhibits the proliferation of SW480 colorectal cancer cells by increase in MAP2K1 and P53 activation. L. casei metallopeptidase under the same circumstances could not affect the growth rate and viability of MDA-MB231 breast cancer cells in vitro.
Apoptosis Cytotoxicity Lactobacillus casei Recombinant plasmid TP53 and MAP2K1 genes,Expression
https://ijbms.mums.ac.ir/article_17738.html
https://ijbms.mums.ac.ir/article_17738_258ec3e422e10e3654c6ee7d53b85887.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
4
2021
04
01
PANC-1 cancer stem-like cell death with silybin encapsulated in polymersomes and deregulation of stemness-related miRNAs and their potential targets
514
523
EN
Fatemeh
Khakinejad
Tehrani
0000-0001-7768-5026
Department of Biology, Faculty of Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran
maryamtehrani86@gmail.com
Najmeh
Ranji
0000-0001-5233-2427
Department of Biology, Faculty of Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran
najmehranji@gmail.com
Fatemeh
Kouhkan
0000-0001-7262-0207
Department of Biology, Faculty of Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran
f.kouhkan@stemcells.tec
Simzar
Hosseinzadeh
0000-0001-8749-2447
Department of Biology, Faculty of Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran
s.hosseinzadeh@sbmu.ac.ir
10.22038/ijbms.2021.54001.12136
<em><strong>Objective(s):</strong></em> Cancer stem cells (CSCs) have powerful self-renewal ability and tumor recurrence. Pancreatic ductal adenocarcinoma is a malignancy with high mortality rate and ˃5% survival. Silybin has anticancer and hepatoprotective properties. We loaded silybin in PEG400-OA (SPNs) and evaluated its cytotoxic effects on PANC-1 cells and PANC-1 CSCs. <br /><em><strong>Materials and Methods</strong></em>: Spheroids from PANC-1 cells were obtained by the hanging drop method. Anti-proliferative and apoptotic functions of SPNs were evaluated in spheroids and non-spheroids with MTT, DNA fragmentation, PI and PI/AnnexinV assays. The expression of CD markers was assessed with flow cytometry. QRT-PCR was used to evaluate the expression of some miRNAs and targets. <br /><em><strong>Results:</strong></em> IC50 of SPNs was identified to be 50 µg/ml, 45 µg/ml, and 42µg/ml, respectively after 24 hr, 48 hr, and 72 hr in PANC-1 treated cells. PI staining and PI/AnnexinV assay showed that ~20%, ~60%, and ~80%, of cells treated with 30, 50, and 60 µg/ml of SPNs were in sub-G1 and apoptosis phase, respectively. DNA degradation was confirmed after SPNS stimulation. CD24, CD44, and CD133 expression decreased after SPNs treatment both in PANC-1 spheroid cells and PANC-1 cancer cell line. Under-expression of onco-miRs (miR-21, miR-155, and miR-221), over-expression of several apoptotic potential targets of oncomiRs (Bax, Casp-9, and P53), over-expression of tumor suppressive-miRs (let-7b, miR-34a, and miR-126), and under-expression of Bcl-2 was found in SPNs-treated cells. <br /><em><strong>Conclusion:</strong></em> We suggest that silybin encapsulated in polymersomes (SPNs) may be useful as a complementary agent for destroying both pancreatic cancer cells and pancreatic CSCs along with chemotherapeutic agents.
Cancer Stem cell,miRNA,Pancreatic Cancer,Polymersome,Silybin
https://ijbms.mums.ac.ir/article_17742.html
https://ijbms.mums.ac.ir/article_17742_9da335ff75b8c39badf5c1d7ebf88fdc.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
4
2021
04
01
Investigating the basis for the antidepressant effects of Gleditsiae spina using an integrated metabolomic strategy
524
530
EN
Tong
Liu
School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, China
liutong00021@163.com
Ning
Zhou
0000-0002-3351-5532
School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, China
zhoun0813@163.com
Yangang
Cao
0000-0002-6744-5974
School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, China
yxycyg@hactcm.edu.cn
Ruihao
Xu
School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, China
582680048@qq.com
Zhen
Liu
School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, China
1115171023@qq.com
Xiaoke
Zheng
0000-0003-3671-231X
School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, China
zhengxk.2006@163.com
Weisheng
Feng
School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, China
fwsh@hactcm.edu.cn
10.22038/ijbms.2021.51975.11781
<em><strong>Objective(s):</strong></em> Gleditsiae spina (GS) is a natural antidepressant but its mechanisms of action remain unclear. In the present study, taxifolin (Tax) was selected to determine the role of flavonoids in the antidepressant effects of GS.<br /><em><strong>Materials and Methods:</strong> </em>Urine samples from C57BL/6 mice were analyzed based on ultra performance liquid chromatography-quadrupole time of flight mass spectrometry (UPLC-Q/TOF-MS). Then, we investigated the therapeutic effects of GS and Tax in depression models in vivo. An integrated metabolomic approach was used to examine the metabolic profiles of GS/Tax groups and corticosterone model groups (Cor). Metabolic networks in response to GS/Tax treatment were established for the comparison of antidepressant activities.<br /><em><strong>Results:</strong></em> Corticosterone exposure significantly increased serum levels of corticosterone but decreased serum levels of 5-hydroxytryptamine and sucrose consumption (p <0.01). Treatment with GS and Tax improved all measured variables compared to those of the corticosterone-exposed group (p < 0.01). The antidepressant effects of GS and Tax involved the regulation of pentose and glucuronate interconversions, arginine and proline metabolism, phenylalanine metabolism, taurine and hypotaurine metabolism, and the citrate cycle.<br /><em><strong>Conclusion:</strong></em> These findings indicate that flavonoids form the pharmacodynamic basis of the antidepressant effects of GS. Moreover, our findings highlight that integrated metabolomics provides a powerful tool to study the mechanisms and material basis of Chinese herbs.
Biomarker,Depression,Flavonoids,Metabolomics,Metabolic profiling
https://ijbms.mums.ac.ir/article_17737.html
https://ijbms.mums.ac.ir/article_17737_f7da195fc293cde5dabec8ad158dfdd4.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
4
2021
04
01
Novel function of Nanos2 in expression of innate immunity genes and its probable roles in maintenance of pluripotency state
531
536
EN
Monireh
Bahrami
0000-0001-5646-1760
Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
monireh.bahrami@yahoo.com
Maryam
Moghaddam Matin
0000-0002-7949-7712
Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
matin@um.ac.ir
Moein
Farshchian
0000-0002-1228-048X
Stem Cell and Regenerative Medicine Research Group, Iranian Academic Center for Education, Culture and Research (ACECR) Razavi Khorasan, Mashhad Branch, Iran
moeinfarshchy@yahoo.com
Molood
Asadi
Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
molood.asadi93@gmail.com
Ahmad Reza
Bahrami
0000-0001-9122-7216
Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
ar-bahrami@um.ac.ir
10.22038/ijbms.2021.53841.12104
<em><strong>Objective(s):</strong></em> Cell-based therapeutic approaches have witnessed significant developments during the last decade especially after approval of MSCs based treatment of graft versus host disease. Several cell-based approaches have shown immunomodulatory behavior during regeneration following the unknown cascade of events but the exact mechanisms are yet to be defined. Clinical applications of cell-based drugs are hampered all over the world because of incomplete understanding of molecular mechanisms requiring the application of mechanistic approaches to solving the mystery. Current work has given us the idea that Nanos2 enhances the cellular pluripotency characteristics while down-regulating the innate immunity genes, simultaneously.<br /><em><strong>Materials and Methods:</strong></em> The immunomodulatory behavior of cells was studied against cells carrying the ectopic expression of Nanos2 in comparison with Stella and Oct4 individually and simultaneously using SON vector (Stella, Nanos2 and Oct4). <br /><em><strong>Results:</strong></em> It was observed that overexpression of Nanos2 leads to down-regulation of Interferon-Stimulated Genes (ISGs)-mRNAs such as Ifitm1, lsg15, Oas2, and Oas12. Nanos2 overexpressing MEF cells have shown restrictive inflammatory effects when cells were treated with inflammatory stimuli such as LPS and Poly (I:C). <br />Conclusion: From our recent findings in line with many others, it can be concluded that Nanos2 acts as a coin with two sides, regulating pluripotency and immunity together which enhances resistance against inflammatory stimuli. Nanos2 could be a potential candidate as a molecular drug for management of inflammation and immunomodulation but it requires a comprehensive comparative expression analysis of innate immunity genes in vitro and in vivo.
Immunomodulatory therapy,Inflammation,Innate immunity,Regenerative medicine,Stem cell transplantation
https://ijbms.mums.ac.ir/article_17740.html
https://ijbms.mums.ac.ir/article_17740_54820b63d0a62980506712c49ab24603.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
4
2021
04
01
Down-regulation of immune checkpoints by doxorubicin and carboplatin-containing neoadjuvant regimens in a murine breast cancer model
537
544
EN
Sanambar
Sadighi
0000-0001-7803-6802
Department of Medical Oncology, Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran
s.sadighi@tums.ac.ir
Ramezanali
Sharifian
Department of Hematology and Oncology, Vali-e-Asr Hospital, Tehran University of Medical Sciences, Tehran, Iran
sharifir@sina.tums.ac.ir
Monireh
Kazemimanesh
Department of Molecular Virology, Pasteur Institute of Iran, Tehran, Iran and Université Toulouse III Paul Sabatier, INSERM U1037, Cancer Research Centre of Toulouse (CRCT), Toulouse, France
kazemim1362@yahoo.com
Ahad
Muhammadnejad
Cancer Biology Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences
mohamad.najad@yahoo.com
Zahra
Shohosseini
Department of Medical Biotechnology, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran
shahosseinizahra@gmail.com
Saeid
Amanpour
Cancer Biology Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences
amanpour-s@tums.ac.ir
Samad
Muhammadnejad
0000-0001-9365-7332
Gene Therapy Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran
s-muhammadnejad@tums.ac.ir
10.22038/ijbms.2021.54383.12221
<em><strong>Objective(s):</strong></em> Immune checkpoint expression on tumor-infiltrating lymphocytes (TILs) has a correlation with the outcome of neoadjuvant chemotherapy (NAC) in breast cancer. However, the reciprocal effect of these regimens on the quality and quantity of immune checkpoints has hitherto not been addressed. We aimed to evaluate the impact of three NAC regimens on TILs and immune checkpoints in a murine triple-negative breast cancer model.<br /><em><strong>Materials and Methods:</strong></em> Syngeneic model of locally-advanced breast cancer was established in immunocompetent mice using a 4T1 cell line. Tumor-bearing animals were treated with human-equivalent dosages of doxorubicin, paclitaxel, paclitaxel and carboplatin combination, and placebo. Infiltration of CD3+, CD8+, and FoxP3+ cells into the tumor was assessed by immunohistochemistry. Expression of immune checkpoints, including PD-1, CTLA-4, and TIM-3, was evaluated by real-time PCR.<br /><em><strong>Results:</strong></em> Doxorubicin led to a significant (p <0.01) increase in the percentage of the stromal infiltrating CD3+ and CD8+ lymphocytes. Doxorubicin also suppressed significantly (p <0.05) the relative expression of PD-1 compared with the placebo. PD-1 expression was significantly (p <0.05) lower in the group treated with paclitaxel and carboplatin combination as compared with the placebo. The relative expression of TIM-3 was significantly (p <0.05) suppressed in doxorubicin-treated mice in comparison with other interventions.<br /><em><strong>Conclusion:</strong></em> Our findings hypothesize that NAC with doxorubicin may potentiate antitumor immunity not merely by recruitment of TILs, but via down-regulation of PD-1 and TIM-3 checkpoints. Carboplatin-containing NAC may suppress PD-1 as well.
Animal model Breast neoplasms Immune checkpoints Neoadjuvant chemotherapy Tumor,infiltrating,Lymphocytes
https://ijbms.mums.ac.ir/article_17741.html
https://ijbms.mums.ac.ir/article_17741_3fcd7daa042476d8fe4f8f5c5ed30319.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
24
4
2021
04
01
Fast antibody responses by immuno-targeting and nanotechnology strategies versus HBsAg vaccine
545
550
EN
Mahsa
Rezaei
Department of Biology, Sciences and Research Branch, Islamic Azad University, Tehran, Iran
mahsa.rezaei1369@yahoo.com
Seyed Nezamedin
Hosseini
Department of Hepatitis B Vaccine Production, Production and Research Complex, Pasteur Institute of Iran, Tehran, Iran
seyyednezam@yahoo.com
Ramazan Ali
Khavari-Nejad
Department of Biology, Sciences and Research Branch, Islamic Azad University, Tehran, Iran
khavarinejad@tum.ac.ir
Farhood
Najafi
0000-0003-3094-1322
Department of Resin and Additives, Institute for Color Science and Technology, Tehran, Iran
farhoodnajafi@yahoo.com
Mehdi
Mahdavi
0000-0003-4478-5957
Recombinant Vaccine Research Center, Tehran University of Medical Sciences, Tehran, Iran
mahdavivac@gmail.com
10.22038/ijbms.2021.52715.11896
<em><strong>Objective(s):</strong></em> Though immunization with HBsAg has been routine since the 1980s, it has numerous limitations such as low or none humoral immune responses. Today, nanotechnology is used in vaccinology to achieve higher potency. The present study deals with the achievement of fast antibody response of humoral immune responses using immune-targeting through mannosylated nanocarriers of the vaccine.<br /><em><strong>Materials and Methods:</strong></em> Mannose sugar and HBsAg were attached to the surface of iron oxide nanoparticles. Mannosylated iron oxide nanoparticles conjugated HBsAg (HBsAg +MLCMNP), iron oxide nanoparticles conjugated HBsAg (HBsAg +LCMNP), hepatitis B vaccine, and mere HBsAg were injected twice to BALB/c mice subcutaneously, while suitable control groups were considered. Specific total IgG antibodies were evaluated on the 7th and 14th days after the final immunization. The avidity maturation of the humoral immune response was assessed with an optimized ELISA. Graph pad prism software was used to analyze statistical data. <br /><em><strong>Results:</strong></em> Results showed that on the seventh day of the final shooting, the mannosylated nano-vaccine caused higher antibody response induction than nano-vaccine without mannose and commercial vaccine groups. After 14 days of the second injection, a significant difference was seen versus the nano-vaccine without mannose but not the commercial vaccine group. In addition, the avidity index in mannosylated nano-vaccine showed a significant increase compared with the nano-vaccine without mannose and mere HBsAg group but not compared with the commercial vaccine.<br /><em><strong>Conclusion:</strong></em> It seems that mannosylated nano-vaccine has more potency to achieve fast antibody responses and also higher quality of humoral immune response.
Avidity index,Hepatitis B,Immune response,Iron oxide nanoparticle,Mannose
https://ijbms.mums.ac.ir/article_17757.html
https://ijbms.mums.ac.ir/article_17757_dd491c852a6b8959dec7f51b16a34f6f.pdf