Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
17
6
2014
06
01
Production and characterization of polyclonal antibody against a synthetic peptide from β-actin protein
396
400
EN
Nazila
Amini
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Mohadeseh
Naghi Vishteh
Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Omid
Zarei
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
omidzarei58@gmail.com
Reza
Hadavi
Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
reza_jahrom@yahoo.com
Negah
Ahmadvand
Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Hodjattallah
Rabbani
Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
hodrab@gmail.com
Mahmood
Jeddi-Tehrani
Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
n.amini60@yahoo.com
10.22038/ijbms.2014.2922
<em>Objective(s)</em>:Antibodies against actin, as one of the most widely studied structural and multifunctional housekeeping proteins in eukaryotic cells, are used as internal loading controls in western blot analyses. The aim of this study was to produce polyclonal antibody against a synthetic peptide derived from N-terminal region of β-actin protein to be used as a protein loading control in western blot and other assay systems. <br/><em>Materials and Methods</em>: A synthetic peptide derived from β-actin protein was designed and conjugated to Keyhole limpet hemocyanin (KLH (and used to immunize a white New Zealand rabbit. The antibody was purified from serum by affinity chromatography column. The purity of the antibody was determined by SDS-PAGE and its ability to recognize the immunizing peptide was measured by ELISA. The reactivity of the antibody with β-actin protein in a panel of different cell lysates was then evaluated by western blot. In addition, the reactivity of the antibody with the corresponding protein was also evaluated by Immunocytochemistry and Immunohistochemistry in different samples. <br/><em>Results</em>: The antibody could recognize the immunizing peptide in ELISA. It could also recognize β-actin protein in western blot as well as in immunocytochemistry and immunohistochemistry. <br/><em>Conclusion</em>: Our data suggest that this antibody may be used as an internal control in western blot analyses as well as in other immunological applications such as ELISA,immunocytochemistry and immunohistochemistry.
antibody,β-actin Immunocytochemistry,Immunohistochemistry Peptide,Western blot
https://ijbms.mums.ac.ir/article_2922.html
https://ijbms.mums.ac.ir/article_2922_9b8238e0d5e29ca4a3e62f744be2bbde.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
17
6
2014
06
01
Comparison of the genetic convergence between myco-bacterium strains by three RFLP-based methods in central province of Iran
401
405
EN
Aliasghar
Farazi
Department of Infectious Diseases, Arak University of Medical Sciences, Arak, Iran
farazialiasghar@yahoo.com
Mansoureh
Jabbariasl
Department of Disease Control and Prevention, Arak University of Medical Sciences, Arak, Iran
jabbariasl@yahoo.com
Keyvan
Tadayon
Department of tuberculin Production, Razi Vaccine and Serum Research Institute, Karaj, Iran
k.tadayon@rvsri.ir
Nader
Mossavar
Department of tuberculin Production, Razi Vaccine and Serum Research Institute, Karaj, Iran
nmosavari@yahoo.com
Roohollah
Keshavarz
Department of tuberculin Production, Razi Vaccine and Serum Research Institute, Karaj, Iran
piroozkeshavarz@gmail.com
Seyed davood
Hoseini
Department of tuberculin Production, Razi Vaccine and Serum Research Institute, Karaj, Iran
hosseinida@yahoo.com
Nilüfer
Orhan
Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Hipodrom, Ankara, Turkey
Didem
Deliorman Orhan
Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Hipodrom, Ankara, Turkey
didemdeliorman@gmail.com
Mustafa
Aslan
Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Hipodrom, Ankara, Turkey
maslan1969@gmail.com
Fatma
Ergun
Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Hipodrom, Ankara, Turkey
fergun@gazi.edu.tr
10.22038/ijbms.2014.2923
<br/><em>Objective(s)</em>:The utilization of molecular techniques in the epidemiology of tuberculosis have provided an opportunity for using effective markers to trace the transmission of the disease .The purpose of this study was to compare the genetic patterns of <em>Mycobacterium tuberculosis</em> by three methods of RFLP technique. <br/><em>Materials and Methods</em>: In a cross-sectional and prospective study, 95 strains of <em>M. tuberculosis</em> isolates were selected for DNA fingerprinting. Extraction of DNA from <em>Mycobacterium</em> strains and DNA fingerprinting with IS-6110, PGRS and DR probe were performed by standard protocols. <br/><em>Results</em>:Overall, the diversity of RFLP among 95 tuberculosis patients were 48, 50 and 45 on the basis of IS6110, PGRS and DR patterns, respectively. Twenty of these patterns (21.1%) with IS6110-RFLP, twenty-two (23.2%) with PGRS-RFLP and seventeen (17.9%) with DR-RFLP occurred with unique RFLP patterns, whereas the remaining 28 patterns were communal. The risk factors of clustering among tuberculosis patients were age < 45 years, new cases, degree of sputum smear ≥ 2+, and close contact. <br/><em>Conclusion</em>: Our study demonstrated that IS6110-RFLP, PGRS-RFLP and DR-RFLP genotyping could roughly identify similar proportions of clustered (secondary) cases as well as the same risk factors for clustering.
DR-RFLP,Genetic convergence,IS6110-RFLP,Mycobacterium tuberculosis PGRS-RFLP
https://ijbms.mums.ac.ir/article_2923.html
https://ijbms.mums.ac.ir/article_2923_fabeb0ac5fe17e1f1a91d07489d88491.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
17
6
2014
06
01
The effects of cyclooxygenase inhibitors on the gastric emptying and small intestine transit in the male rats following traumatic brain injury
406
410
EN
Zakieh
Keshavarzi
Department of Physiology, North Khorasan University of Medical Sciences, Bojnurd, Iran
Mohammad
Khaksari
0000-0000-0000-0000
Department of Physiology, Kerman University of Medical Sciences, Kerman, Iran
khaksar38@yahoo.co.uk
Nader
Shahrokhi
Department of Physiology, Kerman University of Medical Sciences, Kerman, Iran
10.22038/ijbms.2014.2924
<em>Objective(s):</em>This study was carried out to investigate the effects of COX-2 selective inhibitor (Celecoxib) or non-selective COX inhibitor (Ibuprofen) on gastrointestinal motility. <br/><em>Materials and Methods:</em> The rats were randomly divided into five groups including: intact, sham, traumatic brain injury (TBI) group (intact rats under TBI), Celecoxib group (10 mg/kg), Ibuprofen group (10 mg/kg). Rats of the treatment groups received gavages at 1 hr before the TBI induction. The TBI was moderate and diffused using the Marmarou method. The gastric emptying and small intestine transit were measured by phenol red method. <br/><em>Results:</em> The gastric emptying didn’t change following TBI induction compared to intact group. The consumption of ibuprofen or celecoxib didn’t have any effect on gastric emptying compared to sham group. TBI induction didn’t have any effect on the intestinal transit. Also, there was no significant difference between ibuprofen or celecoxib consumption vs. sham group (<em>P</em>>0.05). <br/><em>Conclusion:</em> The COX-2 selective inhibitor (celecoxib) or non-selective COX inhibitor (ibuprofen) have no effects on gastric or small bowel transit. Further work is necessary to investigate the effects of non-selective COX inhibitors and their impact on gastrointestinal motility disorders.
Brain injury,Celecoxib,Gastric Emptying,Ibuprofen
https://ijbms.mums.ac.ir/article_2924.html
https://ijbms.mums.ac.ir/article_2924_16dbd637afec3c3239860a395de2a9c2.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
17
6
2014
06
01
The effects of nano-silver and garlic administration during pregnancy on neuron apoptosis in rat offspring hippocampus
411
418
EN
Maryam
Lale Ataei
Department of Anatomy and Cell biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Ali Reza
Ebrahimzadeh-bideskan
Department of Anatomy and Cell biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
10.22038/ijbms.2014.2925
<em>Objective(s)</em>:The aim of this study was to investigate the effects of nano-silver and garlic administration during pregnancy on neuron apoptosis in rat offspring hippocampus. <br/><em>Materials and Methods:</em> Fifty pregnant wistar rats were randomly divided into five groups: 1- nano- silver (N.S) group; 30 mg/kg of N.S treated via gavage. 2- Control (C) group, administrated with distilled water via gavage. 3- N.S and garlic (N.S+G) group; N.S (30 mg/kg) and garlic juice (1 ml/100 g) treated via gavage simultaneously. 4- Garlic group (G); garlic juice (1 ml/100 g) administrated via gavage, 5- normal (N) without any intervention. All the interventions were done during pregnancy (21 days). Finally, the brains of rat offspring were removed to use for nano-silver level measurement and TUNEL staining. The mean of TUNEL positive cell numbers per unit area (N<sub>A</sub>) in different regions of hippocampus were compared in all animal groups. <br/><em>Results:</em> The results revealed a significant increase of hippocampus nano-silver level in N.S and N.S+G groups comparing to N group (<em>P</em><0.05) and a significant decrease in nano-silver level in N.S+G group comparing to N.S group (<em>P</em><0.01). The number of TUNEL positive cells in the CA1, CA3, and DG fields of rat offspring hippocampus increased in N.S and N.S+G groups comparing to other ones, and also reduced significantly in N.S+G group comparing to N.S group (<sup>¥ </sup> <em>P</em>< 0.01). <br/><em>Conclusion:</em> Our results showed that co-administration of nano-silver and garlic during pregnancy may lead to reduce nano-silver induced apoptotic cells in their offspring hippocampus.
Apoptosis,Garlic,Hippocampus,Nano-silver
https://ijbms.mums.ac.ir/article_2925.html
https://ijbms.mums.ac.ir/article_2925_14e0bf3eb79fedb7fb4c97a1b556df18.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
17
6
2014
06
01
The effects of tramadol on norepinephrine and MHPG releasing in locus coeruleus in formalin test in rats: a brain stereotaxic study
419
425
EN
Mohammad Ali
Mobasher
1 Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
mobasherm@sums.ac.ir
Javad
Sajedianfard
Department of Physiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
jsajedianfard@gmail.com
Akram
Jamshidzadeh
Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
reza.heidari@hotmail.com
Naser
Naghdi
Department of Physiology and Pharmacology, Institute Pasture of Iran, Tehran, Iran
nnaghdiir@yahoo.com
Mohammad Mehdi
Namvaran
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
mmehdi_namvaran@yahoo.com
10.22038/ijbms.2014.2926
<em>Objective(s):</em>The relationship between tramadol, as an antinociceptive drug, and locus coeruleus (LC), the main noradrenergic nucleus of the brain that affects regulation and modulation of pain through descending noradrenergic pathways was investigated. <br/><em>Materials and Methods:</em> Male Sprague-Dawley rats were divided into four groups of 10 rats. The rats were fixed in stereotaxic instrument and then a probe was inserted into LC. Pain was induced by subcutaneous injection of 50 μl of 2.5% formalin 40 minutes after initiation of microdialysisin right hind paw, and nociceptivepain scores were calculated every 5 minutes. Subsequently noradrenaline (NA) and its metabolite, 3-methoxy-4-hydroxyphenylglycol (MHPG), were collected and measured by microdialysis of locus coeruleus in freely moving rats every 15 minutes during formalin injection. <br/><em>Results:</em> Nociceptive pain scores observed in formalin test had the highest nociceptive sensation 5 minutes after injection. Significant rises in concentrations of NA and MHPG, in samples taken between 30 and 45 min after initiation of the locus coeruleusmicrodialysis, coincided with the peak of the pain after injection of formalin. <br/><em>Conclusion:</em> According toconcurrency of the highest nociceptive sensation and peak of NE and MHPG concentrations, tramadol can indirectly affect the LC by blocking the pain signals from different parts of the brain such as periaqueductal gray mater, central nucleus of amygdale or the spinal cord.
Antinociceptive,Locus coeruleus,Microdialysis,Stereotaxic instrument,Tramadol
https://ijbms.mums.ac.ir/article_2926.html
https://ijbms.mums.ac.ir/article_2926_9105b316871d19fcb527245b2cbc7093.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
17
6
2014
06
01
Enzyme inhibitory and radical scavenging effects of some antidiabetic plants of Turkey
426
432
EN
Nilüfer
Orhan
Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Hipodrom, Ankara, Turkey
Sanem
Hoşbaş
Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Hipodrom, Ankara, Turkey
snmhsbs@yahoo.com
Didem
Deliorman Orhan
Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Hipodrom, Ankara, Turkey
didemdeliorman@gmail.com
Mustafa
Aslan
Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Hipodrom, Ankara, Turkey
maslan1969@gmail.com
Fatma
Ergun
Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Hipodrom, Ankara, Turkey
fergun@gazi.edu.tr
10.22038/ijbms.2014.2927
<em>Objective(s)</em>:Ethnopharmacological field surveys demonstrated that many plants, such as <em>Gentiana olivieri</em>, <em>Helichrysum graveolens</em>, <em>Helichrysum plicatum</em> ssp. <em>plicatum</em>, <em>Juniperus oxycedrus</em> ssp. <em>oxycedrus</em>, <em>Juniperus communis </em>var.<em> saxatilis</em>, <em>Viscum album </em>(ssp. <em>album, </em>ssp.<em> austriacum</em>), are used as traditional medicine for diabetes in different regions of Anatolia. The present study was designed to evaluate the <em>in vitro</em> antidiabetic effects of some selected plants, tested in animal models recently. <br/><em>Materials and Methods:</em> <em>α</em>-glucosidase and <em>α</em>-amylase enzyme inhibitory effects of the plant extracts were investigated and Acarbose was used as a reference drug. Additionally, radical scavenging capacities were determined using 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) ABTS radical cation scavenging assay and total phenolic content of the extracts were evaluated using Folin Ciocalteu method. <br/><em>Results:</em><em> H. graveolens </em>ethanol extract exhibited the highest inhibitory activity (55.7 % ± 2.2) on <em>α</em>-amylase enzyme. Additionally, <em>J. oxycedrus</em> hydro-alcoholic leaf extract had potent <em>α-</em>amylase inhibitory effect, while the hydro-alcoholic extract of <em>J. communis</em> fruit showed the highest <em>α</em>-glucosidase inhibitory activity (IC<sub>50</sub>: 4.4 μg/ml). <br/><em>Conclusion:</em>Results indicated that, antidiabetic effect of hydro-alcoholic extracts of <em>H. graveolens capitulums, J. communis</em> fruit and <em>J. oxycedrus</em> leaf might arise from inhibition of digestive enzymes.
α-Amylase,α-Glucosidase,Gentiana olivier,Helichrysum,Juniperus,Viscum album
https://ijbms.mums.ac.ir/article_2927.html
https://ijbms.mums.ac.ir/article_2927_7371e14044edda934718ce3fe3e94c44.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
17
6
2014
06
01
Gender difference in motor impairments induced by chronic administration of vinblastine
433
440
EN
Shahrnaz
Parsania
Physiology Research Center, Neuropharmacology Institute, Kerman University of Medical Sciences, Kerman, Iran
parsania_shahrnaz@yahoo.com
Mohammad
Shabani
Kerman Neuroscience Research Center, Neuropharmacology Institute, Kerman University of Medical Sciences, Kerman, Iran
shabanimoh@gmail.com
Kasra
Moazzami
Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02129, USA
kasra.moazzami@gmail.com
Moazamehosadat
Razavinasab
Physiology Research Center, Neuropharmacology Institute, Kerman University of Medical Sciences, Kerman, Iran
razavimoaz@yahoo.com
Mohammad Hassan
Larizadeh
Department of Radiation Oncology, Kerman University of Medical Sciences, Kerman, Iran
larizad_mh@yahoo.com
Masoud
Nazeri
Student research committee, Kerman University of Medical Sciences, Kerman, Iran
m.nazeri1989@gmail.com
Majid
Asadi-Shekaari
Kerman Neuroscience Research Center, Neuropharmacology Institute, Kerman University of Medical Sciences, Kerman, Iran
majidasadi@kmu.ac.ir
Moein
Kermani
Student research committee, Kerman University of Medical Sciences, Kerman, Iran
m.kermani1991@gmail.com
10.22038/ijbms.2014.2928
<em>Objective(s):</em>Neurotoxicity of anticancer drugs complicates treatment of cancer patients. Vinblastine (VBL) is reported to induce motor and cognitive impairments in patients receiving chronic low-dose regimen. <br/><em>Materials and Methods:</em> The effects of VBL treatment on motor, learning and memory functions of male and female Wistar rats were studied by behavioral related tests. Animals were given chronic intraperitoneal injections of VBL (0.2 mg/kg/week for 5 weeks) from postnatal day 23 to 52. Motor function was evaluated using grasping test and balancing was evaluated by the rotarod. Spatial learning and memory and anxiety-like behavior were determined using Morris water maze (MWM) task and open field test, respectively. <br/><em>Results:</em> Administration of VBL caused severe damage to motor and balance function of male rats in comparison to female rats treated with VBL and rats treated with saline. Memory and locomotion were affected in both male and female rats compared with saline treated rats, while a sex difference was also observed in these parameters; male rats showed more impairment compared with female ones. Both male and female rats showed cognitive impairments in MWM task and no sex differences were observed in these functions. <br/><em>Conclusion:</em> Results revealed that VBL is a potent neurotoxic agent and despite the profound effect of VBL on motor and cognitive functions, it seems that male rats are more susceptible to motor deficits induced by VBL.
Anticancer,Learning and memory,motor function,Vinblastine
https://ijbms.mums.ac.ir/article_2928.html
https://ijbms.mums.ac.ir/article_2928_16ee86a3671ca0857a86cb6c53c4e652.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
17
6
2014
06
01
Frequencies of two functionally significant SNPs and their haplotypes of organic anion transporting polypeptide 1B1 SLCO1B1 gene in six ethnic groups of Pakistani population
441
447
EN
Tausif Ahmed
Rajput
Department of Biochemistry and Molecular Biology, Centre for Research in Experimental and Applied Medicine (CREAM), Army Medical College, National University of Sciences & Technology (NUST), Islamabad, Pakistan
Abdul Khaliq
Naveed
Department of Biochemistry and Molecular Biology, Centre for Research in Experimental and Applied Medicine (CREAM), Army Medical College, National University of Sciences & Technology (NUST), Islamabad, Pakistan
Shakir
Khan
Department of Biochemistry and Molecular Biology, Centre for Research in Experimental and Applied Medicine (CREAM), Army Medical College, National University of Sciences & Technology (NUST), Islamabad, Pakistan
drshakirkhan@hotmail.com
Zia-Ur
Rehman Farooqi
Department of Biochemistry and Molecular Biology, Centre for Research in Experimental and Applied Medicine (CREAM), Army Medical College, National University of Sciences & Technology (NUST), Islamabad, Pakistan
10.22038/ijbms.2014.2929
<em>Objective(s):</em>Organic anion transporter polypeptide 1B1 (OATP1B1) encoded by solute carrier organic transporter 1B1 (<em>SLCO1B1</em>) gene; a transporter involved in the uptake of drugs and endogenous compounds is present in hepatocyte sinusoidal membrane. Aim of this study was to investigate the frequencies of functionally significant SNPs (388A>G and 521T>C) and their haplotypes in 6 ethnic groups of Pakistani population through the development of rapid and efficient Tetra amplification refractory mutation system (T. ARMS) genotyping assay. <br/><em>Materials and Methods:</em> Frequencies of alleles, genotype, and haplotypes of two functionally significant Single nucleotide polymorphism in 180 healthy Pakistani subjects and distributions in six ethnic groups by using a single step T. ARMS genotyping assay. <br/><em>Results:</em> The allelic frequency for 388A>G SNP was 50% in total Pakistani population with distributions of 9.7%, 15.1%, 19.4%, 16.1%, 18.3%, and 21.5% in Punjabi, Sindhi, Balouchi, Pathan, Kashmiri and Hazara/Baltistan groups respectively; and for 521T>C SNP it was 23.9% in total Pakistani population with distributions of 11.1%, 8.9%, 15.6%, 11.1%, 31.1% and 22.2% in Punjabi, Sindhi, Balouchi, Pathan, Kashmiri, and Hazara/Baltistan groups. Both functionally significant SNPs occurred in four major haplotypes with a frequency of 35.5% for 388A/521T (<em>*1A</em>), 40.5% for 388G/521T (<em>*1B</em>), 14.4% for 388A/521C (<em>*5</em>), and 9.4% for 388G/521C (<em>*15</em>) with varying distributions among six ethnic groups. <br/><em>Conclusion:</em> The 388A>G and 521T>C genotypes and corresponding haplotypes are present at varying frequencies in various ethnic groups of Pakistani population. Pharmacokinetic and pharmacodynamic
Haplotype,OATP1B1,single nucleotide polymorphism,SLCO1B1
https://ijbms.mums.ac.ir/article_2929.html
https://ijbms.mums.ac.ir/article_2929_abeca501d9b5d89c21ffc66a9cc6fcfc.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
17
6
2014
06
01
Pantoprazole, a proton pump inhibitor, increases orthodontic tooth movement in rats
448
453
EN
Mohsen
Shirazi
Dental Research Center, Tehran University of Medical Sciences, Tehran, Iran
Department of Orthodontics, Faculty of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
mohsenshirazi@irimc.org
Houman
Alimoradi
Department of Pharmacology and Toxicology, University of Otago, P.O. Box 913, Dunedin, New Zealand
houman.alimoradi@gmail.com
Yasaman
Kheirandish
Department of Oral and Maxillofacial Radiology, Faculty of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
kheirandish@tums.ac.ir
Shahroo
Etemad‐Moghadam
Dental Research Center, Tehran University of Medical Sciences, Tehran, Iran
Mojgan
Alaeddini
Dental Research Center, Tehran University of Medical Sciences, Tehran, Iran
malaeddini@yahoo.com
Alipasha
Meysamie
Department of Community Medicine, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
meysamie@tums.ac.ir
Seyed Amir Reza
Fatahi Meybodi
Department of Orthodontics, Faculty of Dentistry, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
fattahihamidi@yahoo.com
Ahmad Reza
Dehpour
0000-0002-8001-5565
Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran
dehpour@yahoo.com
10.22038/ijbms.2014.2930
<em>Objective(s):</em>Pantoprazole, is a proton pump inhibitor (PPI) prescribed for the treatment of upper gastrointestinal disorders, which in high doses has been suggested to decrease calcium absorption leading to hypocalcaemia and therefore osteoporosis. The aim of this study was to assess whether pantoprazol, could alter the rate of orthodontic tooth movement (OTM) in rats. <br/><em>Materials and Methods:</em> A time course study was established using 72 rats which were divided into six groups of 12 samples each (four: vehicle; eight: pantoprazole + vehicle). Pantoprazole at a dose of 200 mg/kg suspended in carboxymethyl cellulose (0.25 percent) was administered by a gastric tube. The upper incisors and first molars were ligated by a 5 mm nickel-titanium closed-coil spring to deliver an initial force of 60 g. Animals were euthanized two weeks after orthodontic treatment followed by assessment of tooth movement and histomorphometric evaluation of the detached maxillae. Lateral skull radiographs were obtained once a week, starting from the first day to the 6<sup>th</sup> week of the study. OTM and bone density data were analyzed using independent sample t-test and repeated measures ANOVA. <br/><em>Results:</em> No significant changes in OTM measurements and optical density were observed in vehicle-receiving animals during the study (<em>P</em>=0.994). OTM was significantly increased after six weeks pantoprazole therapy which continued until the 7<sup>th</sup> week of the experiment (<em>P</em>=0.007). Optical density significantly increased in the pantoprazole-treated rats after six weeks. <br/><em>Conclusion:</em> Long term PPI therapy at high doses could lead to osteoporosis and enhanced OTM.
Pantoprazole,Proton pump inhibitors,Radiography,Rats,Tooth movement
https://ijbms.mums.ac.ir/article_2930.html
https://ijbms.mums.ac.ir/article_2930_9e94db700a477e5242e1cd3e22507324.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
17
6
2014
06
01
Mild hypothermia reduces expression of Fas/FasL and MMP-3 after cerebral ischemia-reperfusion in rats
454
459
EN
Jingkun
Zhao
Department of Neurology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China
301zhao@gmail.com
Shurong
Duan
Department of Neurology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China
zhao.hmufh.medix@gmail.com
Jinxia
Zhou
PuRen Hospital of WuHan, Wu han, China
301zhou@gamil.com
Ruihong
Sun
Department of Neurology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China
aumeidx@gmail.com
Liming
Zhang
Department of Neurology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China
301zhang@gmail.com
Desheng
Wang
Department of Neurology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China
301wang@gmail.com
10.22038/ijbms.2014.2931
<em>Objective(s)</em>:To investigate the effects of local mild hypothermia on the expression of Fas, FasL and MMP-3 after cerebral ischemia-reperfusion in rats. <br/><em>Materials and Methods:</em>Male Wistar rats were divided into sham-operated group (Sham), normothermia group (NT), and hypothermia group (HT). MCAO/R model was established by Longa’s method, and reperfusion was allowed after 2 hr occlusion. Mild hypothermia (33±0.5℃) for 6 hr was initiated at the start of reperfusion. Immunohistochemistry was performed to determine expression Fas, FasL, and MMP-3. <br/><em>Results: </em>Infarct volume was reduced in the hypothermia group (18.43±4.23%) compared with the normothermia group (24.76±5.76%) (<em>P</em><0.05). In mild hypothermia group, numbers of Fas-positive and MMP-3 positive cells were significantly less than those of normothermia group (<em>P</em><0.05). Neurological functional scores of mild hypothermia were significantly improved (<em>P</em><0.05). <br/><em>Conclusion: </em>Mild hypothermia decreases infarct volume after cerebral ischemia-reperfusion, reduces Fas and MMP-3 expression, but increases FasL in cerebral ischemia-reperfusion rats.
Cerebral ischemia/reperfusion,Fas,FasL,Hypothermia,MACO rat,MMP-3
https://ijbms.mums.ac.ir/article_2931.html
https://ijbms.mums.ac.ir/article_2931_b923cc1f5f757de29207887f9de1a18c.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
17
6
2014
06
01
Antioxidant effects of proanthocyanidin from grape seed on hepatic tissue injury in diabetic rats
460
464
EN
Esrafil
Mansouri
Department of Anatomical Sciences, Cellular and Molecular Research Center, Faculty of Medical Sciences, Jundishapur University of Medical Sciences, Ahvaz, Iran
Layasadat
Khorsandi
Department of Anatomical Sciences, Cellular and Molecular Research Center, Faculty of Medical Sciences, Jundishapur University of Medical Sciences, Ahvaz, Iran
lyasadat@yahoo.com
Hassan Ali
Abedi
Department of Physiology, Faculty of Medical Sciences, Jahrom University of Medical Sciences, Jahrom, Iran
10.22038/ijbms.2014.2932
<em>Objective(s):</em>Diabetes plays an important role in the induction of the liver injury. Grape seed proanthocyanidin (GSP) have a wide range of medicinal properties against oxidative stress. In this study we evaluated antioxidant effects of GSP on liver in streptozotocin-induced diabetic rats. <br/><em>Materials and Methods: </em>Thirty male Sprague–Dawley rats were divided into three groups: control, untreated diabetic and diabetic rats treated with GSP. Diabetes was induced in rats by intraperitoneal injection of streptozotocin (50 mg/kg). GSP were administered via oral gavage (200 mg/kg) for 4 weeks. <br/><em>Results:</em> GSP produced significant hepatoprotective effects by decreasing activities of serum aminotransferases and alkaline phosphatase, and decreasing liver malondialdehyde and bilirubin (<em>P</em><0.05) levels. It increased liver superoxide dismutase, catalase and glutathione peroxidase activities and albumin level (<em>P</em><0.05). Administration of GSP significantly ameliorated structural changes induced in liver of diabetic rats. <br/><em>Conclusion:</em> GSP have protective effects against hepatic tissue injury due to antioxidant properties.
Antioxidant enzyme,Diabetes,GSP,Hepatic tissue
https://ijbms.mums.ac.ir/article_2932.html
https://ijbms.mums.ac.ir/article_2932_4b370f73091baabac68fd27b43411d73.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
17
6
2014
06
01
Evaluation of alpha- amylase inhibition by Urtica dioica and Juglans regia extracts
465
469
EN
Mahsa
Rahimzadeh
Molecular Medicine Research Center, Department of Biochemistry, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
Samaneh
Jahanshahi
Molecular Medicine Research Center, Department of Biochemistry, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
Soheila
Moein
Molecular Medicine Research Center, Department of Biochemistry, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
Mahmood Reza
Moein
Medicinal Plant Processing Research Center and Department of Pharmacognosy, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
moeinmr@sums.ac.ir
10.22038/ijbms.2014.2933
<em>Objective(s)</em>:One strategy for the treatment of diabetes is inhibition of pancreatic α- amylase. Plants contains different chemical constituents with potential for inhibition of α-amylase and hence maybe used as therapeutic. <br/><em>Materials and Methods:</em> <em>Urtica dioica </em>and <em>Juglans regia </em>Linn were tested for <em>α</em>-amylase inhibition. Different concentrations of leaf aqueous extracts were incubated with enzyme substrate solution and the activity of enzyme was measured. For determination of the type of inhibition, Dixon plot was depicted. Acarbose was used as the standard inhibitor. <br/><em>Results:</em> Both plant extracts showed time and concentration dependent inhibition of <em>α</em>-amylase. 60% inhibition was seen with 2 mg/ml of <em>U. dioica </em>and0.4 mg/ml of<em> J. regia </em>aqueous extract. Dixon plots revealed the type of <em>α</em>-amylase inhibition by these two extracts as competitive inhibition. <br/><em>Conclusion:</em> Determination of the type of <em>α</em>-amylase inhibition by these plant extracts could provide by successful use of plant chemicals as drug targets.
Amylase inhibition,Diabetes,Plant extract
https://ijbms.mums.ac.ir/article_2933.html
https://ijbms.mums.ac.ir/article_2933_a2a6170dfb40c196c4d864c01c7ebd59.pdf