Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
18
6
2015
06
01
Molecular and genetic aspects of odontogenic tumors: a review
529
536
EN
Kavita
Garg
Department of Oral Pathology, Chandra Dental College, Barabanki, India
drkavitanitish@rediffmail.com
Shaleen
Chandra
Department of Oral Pathology, King George Medical College, Lucknow, India
shal-nam@hotmail.com
Vineet
Raj
Department of Oral Pathology, Saraswati Dental College and Hospital, Lucknow, India
drvineetraj@gmail.com
Wamiq
Fareed
Department of Oral Surgery, Taibah University College of Dentistry, Al Madinah Al Munawwarah Saudi Arabia
wmfareed@gmail.com
Muhammad
Zafar
Department of Restorative Dentistry, Taibah University College of Dentistry, Al Madinah Al Munawwarah, Saudi Arabia
drsohail_78@hotmail.com
10.22038/ijbms.2015.4522
Odontogenic tumors contain a heterogeneous collection of lesions that are categorized from hamartomas to benign and malignant neoplasms of inconstant aggressiveness. Odontogenic tumors are usually extraordinary with assessed frequency of short of 0.5 cases/100,000 population for every year. The lesions such as odontogenic tumors are inferred from the components of the tooth-structuring contraption. They are discovered solely inside the maxillary and mandibular bones. This audit speaks to experiences and cooperation of the molecular and genetic variations connected to the development and movement of odontogenic tumors which incorporate oncogenes, tumor-silencer genes, APC gene, retinoblastoma genes, DNA repair genes, onco-viruses, development components, telomerase, cell cycle controllers, apoptosis-related elements, and regulators/controllers of tooth development. The reasonable and better understanding of the molecular components may prompt new ideas for their detection and administrating a better prognosis of odontogenic tumors.
Genes,Odontogenesis,Odontogenic tumors
https://ijbms.mums.ac.ir/article_4522.html
https://ijbms.mums.ac.ir/article_4522_63816810fdc69594e89151b025bea4cb.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
18
6
2015
06
01
Resveratrol attenuates visfatin and vaspin genes expression in adipose tissue of rats with type 2 diabetes
537
543
EN
Soheila
Asadi
Department of Clinical Biochemistry, Medical School, Hamadan University of Medical Sciences, Hamadan, Iran
sohila.asadi75@yahoo.com
Mohammad Taghi
Goodarzi
0000-0002-0213-9087
Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
mtgoodarzi@yahoo.com
Massoud
Saidijam
Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
sjam110@yahoo.com
Jamshid
Karimi
0000-0001-8253-7124
Department of Clinical Biochemistry, Medical School, Hamadan University of Medical Sciences, Hamadan, Iran
jamshidkarimi2013@gmail.com
Reza
Yadgar Azari
Department of Molecular Medicine and Genetic, Medical School, Hamadan University of Medical Sciences, Hamadan, Iran
reza.yadegarazari@yahoo.com
Azam
Rezaei Farimani
0000-0002-7853-2993
Department of Clinical Biochemistry, Medical School, Hamadan University of Medical Sciences, Hamadan, Iran
azam_rezaei1@yahoo.com
Iraj
Salehi
Chronic Diseases (Home Care) Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
irsalehi@yahoo.com
10.22038/ijbms.2015.4523
<em>Objective(s)</em>: Visfatin and vaspin are secreted by adipose tissue and play key roles in glucose homeostasis and subsequently are potential targets for diabetes treatment. Resveratrol (RVS) corrects insulin secretion and improves insulin sensitivity. We investigated the RVS effects on serum antioxidants, insulin and glucose levels, also visfatin and vaspin genes expression in adipose tissue of streptozotocin-nicotinamide (STZ-NA) induced type 2 diabetic rats. <br/><em>Materials and Methods:</em> Diabetes was induced in Wistar rats (n=32) using STZ (60 mg/kg body weight) and NA (120 mg/kg body weight); rats were divided into 4 groups (n=8). Eight untreated normal rats were used as control group; four diabetic rat groups (2–5) were treated with 0, 1, 5 and 10 mg /kg body weight of RVS, respectively for 30 days. After treatment blood and adipose tissue were prepared from all animals. Serum glucose, insulin, HOMA index, total antioxidant capacity (TAC), and malondialdehyde (MDA) were measured. Visfatin and vaspin genes expression in adipose tissue were evaluated using real-time PCR. <br/><em>Results:</em> RVS reduced blood glucose significantly and increased insulin level, resulting in insulin sensitivity improvement. Furthermore RVS increased weight and TAC, while reducing serum MDA in the diabetic groups. Visfatin gene expression increased in the diabetic group, and RVS treatment reduced it. Vaspin gene expression was reduced in RVS receiving diabetic groups. <br/><em>Conclusion: </em>The results indicated that RVS has potential hypoglycemic effect, probably by increasing insulin level and changing gene expression of visfatin and vaspin. Moreover RVS showed antioxidant effects through reduction in peroxidiation products and augmented antioxidant capacity.
Grape,Insulin sensitivity,Resveratrol,Vaspin,Visfatin
https://ijbms.mums.ac.ir/article_4523.html
https://ijbms.mums.ac.ir/article_4523_670c2d9ae3e9533a208d09f00c910978.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
18
6
2015
06
01
Juglone exerts antitumor effect in ovarian cancer cells
544
548
EN
Fang
Fang
Department of Immunology, Jilin Medical College, Jilin, Jilin, China
Yingxin
Qin
Department of Narcotization, Affiliated Hospital of Jilin Medical College, Jilin, Jilin, China
Ling
Qi
Department of Pathology, Jilin Medical College, Jilin, Jilin, China
Qing
Fang
Department of Immunology, Jilin Medical College, Jilin, Jilin, China
qiling1718@qq.com
Liangzhong
Zhao
Department of Immunology, Jilin Medical College, Jilin, Jilin, China
309781962@qq.com
Shuang
Chen
Department of Immunology, Jilin Medical College, Jilin, Jilin, China
64109122@qq.com
Qiang
Li
Department of Immunology, Jilin Medical College, Jilin, Jilin, China
329383104@qq.com
Duo
Zhang
Department of Immunology, Jilin Medical College, Jilin, Jilin, China
183599146@qq.com
Liguo
Wang
Department of Urology Surgery, Affiliated Hospital of Jilin Medical College, Jilin, Jilin, China
urolancet@sina.com
10.22038/ijbms.2015.4525
<em>Objective(s)</em>: Juglone is isolated from many species of the Juglandaceae family and used as an anti-viral, anti-bacterial, and anti-tumor therapeutic. Here, we evaluated juglone-induced antitumor effect in ovarian cancer SKOV3 cells.
<em>Materials and Methods: </em>MTT assay was performed to examine juglone anti-proliferative effect. Cell cycle and apoptosis were studied using flow cytometry in juglone-treated SKOV3 cells. To investigate molecular mechanism of cell cycle and apoptosis, protein expression levels were measured by Western blot analysis of cyclin D1, Bcl-2, Bax, cytochrome c, caspase-9 and caspase-3. To investigate the motility of juglone-treated SKOV3 cell, Matrigel invasion assay was employed to characterize cell invasion. Also, matrix metalloproteinase-2 (MMP-2) expression levels were detected by western blot.
<em>Results:</em>Juglone significantly inhibited SKOV3 cell proliferation as shown by G0/G1 phase arrest, and this effect was mediated by inactivation of cyclin D1 protein (<em>P</em><0.05). Juglone induced apoptosis in SKOV3 cell which was accompanied by caspase-9 and caspase-3 activation (<em>P</em><0.05). Juglone decreased Bcl-2 levels and increased Bax and cytochrome c (Cyt c) levels(<em>P</em><0.05). Juglone sufficiently inhibited invasion while evidently decreased MMP-2 expression (<em>P</em><0.05).
<em>Conclusion:</em>The results suggest that juglone could probably induce apoptosis through mitochondrial pathway and restrained cell invasiveness by decreasing MMP expression.
Apoptosis,Cell Cycle Arrest,Invasion,Juglone,Ovarian cancer
https://ijbms.mums.ac.ir/article_4525.html
https://ijbms.mums.ac.ir/article_4525_04a34c08957ce0223d3476f06a5c1240.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
18
6
2015
06
01
Effect of selegiline on neural stem cells differentiation: a possible role for neurotrophic factors
549
554
EN
Kambiz
Hassanzadeh
Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran
kambizhassanzadeh@gmail.com
Mehrnoush
Nikzaban
Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran
mehrnoush_nikzaban@yahoo.com
Mohammad
Raman Moloudi
Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran
Esmael
Izadpanah
000000018090906x
Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran
eizadpanah2000@yahoo.com
10.22038/ijbms.2015.4527
<em>Objective(s)</em>: The stimulation of neural stem cells (NSCs) differentiation into neurons has attracted great attention in management of neurodegenerative disease and traumatic brain injury. It has been reported that selegiline could enhance the morphologic differentiation of embryonic stem cells. Therefore this study aimed to investigate the effects of selegiline on NSCs differentiation with focus on the role of neurotrophic factor gene expression.
<em>Materials and Methods:</em> The NSCs were isolated from lateral ventricle of C57 mice brain. The cells were exposed to selegiline in nano to micromolar concentrations for 24 hr or 72 hr. In order to assay the effect of selegiline on NSCs differentiation into neurons, astrocytes and oligodendrocytes, immunocytochemical techniques were utilized. Samples were exposed to specific antibodies against neurons (β tubulin), astrocytes (GFAP) and oligodendrocytes (OSP). The expression of BDNF, NGF and NT3 genes was investigated using Real-Time PCR.
<em>Results:</em> Our findings revealed that selegiline increased NSCs differentiation into neurons at 10<sup>-7</sup> and 10<sup>-8</sup> M and decreased the differentiation into astrocytes at 10<sup>-9</sup><sub>,</sub>while oligodendrocyte did not significantly change in any of the used concentrations. In addition data analyses showed that selegiline increased BDNF, NGF and NT3 gene expression at 24 hr, but did not change them in the other time of exposure (72 hr) except 10<sup>-7</sup> M concentration of selegiline, which increased NT3 expression.
<em>Conclusion:</em>Our results indicate selegiline induced the differentiation of NSCs into neurons and in this context the role of neurotrophic factors is important and should be considered.
Neurotrophic factors,NSCs,Selegiline
https://ijbms.mums.ac.ir/article_4527.html
https://ijbms.mums.ac.ir/article_4527_1024b61329edcbb2858a81b596799793.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
18
6
2015
06
01
Gardenia jasminoides extracts and gallic acid inhibit lipopolysaccharide-induced inflammation by suppression of JNK2/1 signaling pathways in BV-2 cells
555
562
EN
Wen-Hung
Lin
Department of Technology Management, Chung Hua University, Hsinchu, Taiwan
d10103006@chu.edu.tw
Heng-Hung
Kuo
Department of Finance, Chung Hua University, Hsinchu, Taiwan
hhkuo@chu.edu.tw
Li-Hsing
Ho
Department of Technology Management, Chung Hua University, Hsinchu, Taiwan
ho@chu.edu.tw
Ming-Lang
Tseng
Department of Biotechnology, Yuanpei University, Hsinchu, Taiwan
An-Ci
Siao
Office of Physical Education, Chung Hua University Hsinchu, Taiwan
air200447@gmail.com
Chang-Tsen
Hung
Department of Health and Leisure Management, Yuanpei University, Hsinchu, Taiwan
hongct@mail.ypu.edu.tw
Kee-Ching
Jeng
Department of Medical Research, Tungs’ Taichung Metro Harbor Hospital, Taichung, Taiwan
kcjeng@gmail.com
Chien-Wei
Hou
0000-0001-9300-506X
Office of Physical Education, Chung Hua University Hsinchu, Taiwan
rolis.hou@mail.ypu.edu.tw
10.22038/ijbms.2015.4529
<em>Objective(s)</em>: <em>Gardenia jasminoides</em> Ellis (GJ, Cape Jasmine Fruit, Zhi Zi) has been traditionally used for the treatment of infectious hepatitis, aphthous ulcer, and trauma; however, the direct evidence is lacking.
<em>Materials and Methods:</em>We investigated the effect of the GJ extract(GJ) and gallic acid (GA) on lipopolysaccharide (LPS) induced inflammation of BV-2 microglial cells and acute liver injury in Sprague-Dawley (SD) rats.
<em>Results:</em>Our results showed that the GJ extract and GA reduced LPS-induced nitric oxide (NO), interleukin (IL)-1, IL-6, reactive oxygen species (ROS), and prostaglandin (PGE2) production in BV-2 cells. The GJ extract and GA significantly decreased serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels in LPS-treated rats. Furthermore, the water extract, but not the ethanol extract, of the GJ dose-dependently inhibited LPS-induced JNK2/1 and slightly p38 mitogen-activated protein kinases (MAPK), and cyclooxygenase-2 (COX-2) expression in BV-2 cells.
<em>Conclusion:</em>Taken together, these results indicate that the protective mechanism of the GJ extract involves an antioxidant effect and inhibition of JNK2/1 MAP kinase and COX-2 expressions in LPS-induced inflammation of BV-2 cells.
BV-2 cell,Gardenia jasminoides,MAPKs,NO,ROS
https://ijbms.mums.ac.ir/article_4529.html
https://ijbms.mums.ac.ir/article_4529_bc4307afb55d704d2c73bb7a5eb5df70.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
18
6
2015
06
01
Therapeutic angiogenesis promotes efficacy of human umbilical cord matrix stem cell transplantation in cardiac repair
563
570
EN
Kaveh
Moradi
Department of Anatomy, Medical School, Tehran University of Medical Sciences, Tehran, Iran
Mehdi
Abbasi
Department of Anatomy, Medical School, Tehran University of Medical Sciences, Tehran, Iran
abbasima@tums.ac.ir
Farid
Aboulhasani
Department of Anatomy, Medical School, Tehran University of Medical Sciences, Tehran, Iran
Niloufar
Abbasi
Faculty of Medicine, Tehran Medical Branch, Islamic Azad University, Iran
Kehinde
Adebayo Babatunde
Department of Anatomy, Medical School, Tehran University of Medical Sciences, Tehran, Iran
Fereydoon
Sargolzaeiaval
Department of Anatomy, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran
fsargolzaei@yahoo.com
Ahmad-Reza
Dehpour
Department of Pharmacology, Medical School, Tehran University of Medical Sciences, Tehran, Iran
10.22038/ijbms.2015.4530
<em>Objective(s):</em>Although previous studies have confirmed the beneficial effects of human umbilical cord matrix stem cell (hUCM) transplantation post myocardial infarction (MI), but this stem cell resource has no potential to induce angiogenesis. In order to achieve the process of angiogenesis and cardiomyocyte regeneration, two required factors for cardiac repair agents were examined namely; hUCM and VEGF on an infarcted heart. The main objective of this research is to investigate the combinatory effect of dhUCM and VEGF transplantation on an infarcted heart. <br/><em>Materials and Methods:</em>45 min of ligating the left anterior descending coronary artery, the MI-induced <br/>animals received 50 μl PBS, 5 μg VEGF, 5×10<sup>6</sup> hUCM cells alone, combined with 5 μg VEGF and 5×10<sup>6</sup> differentiated hUCM cells alone or combined with 5 μg VEGF through intramyocardial injection. MI group, without hUCM and VEGF served as the control group. Left ventricular function and angiogenesis <br/>were also evaluated. <br/><em>Results: </em>After eight weeks post MI, there were significant rise in left ventricular ejection farction in dhUCM+VEGF group compared to the other treated and non-treated groups (<em>P</em><0.05). Fibrosis tissue was markedly lower in the dhUCM+VEGF and hUCM+VEGF groups compared to the other treated and non-treated groups (<em>P</em><0.05). Despite these benefits, vascular density in dhUCM+VEGF group was not markedly different compared to VEGF and hUCM+VEGF groups. The transplanted hUCM and dhUCM cells survived and migrated to the infarcted area. <br/><em>Conclusion:</em> Our findings demonstrated that the dhUCM cells transplantation combined with VEGF were more efficient on an infarcted heart.
Angiogenesis,Cardiac repair,Human umbilical,Myocardial,Vascular endothelial-growth factor
https://ijbms.mums.ac.ir/article_4530.html
https://ijbms.mums.ac.ir/article_4530_80815718dc001c2903e95d9e66a2205f.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
18
6
2015
06
01
Heterozygosis deficit of polymorphic markers linked to the β-globin gene cluster region in the Iranian population
571
575
EN
Tahereh
Moradi
Division of Genetics, Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran
Reihaneh
Vallian
Molecular Genetics Department, Isfahan Medical Genetics Center, Isfahan, Iran
svallian@isfahangenetics.com
Zahra
Fazeli
0000-0002-9180-1991
Department of Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
zahrafazeli6@gmail.com
Asieh
Haghighatnia
Molecular Genetics Department, Isfahan Medical Genetics Center, Isfahan, Iran
asiehhaghighatnia@yahoo.com
Sadeq
Vallian
0000-0002-5151-5923
Division of Genetics, Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran
sadeqvallian@gmail.com
10.22038/ijbms.2015.4531
<em>Objective(s)</em>: Iran is considered as one of the high-prevalence areas for β-thalassemia with a rate of about 10% carrier frequency. Molecular diagnosis of the disease is performed both by direct sequencing and indirectly by the use of polymorphic markers present in the beta globin gene cluster. However, to date there is no reliable information on the application of the markers in the Iranian population. Here we report the results of an extended molecular analysis of five RFLP markers,<em> Xmn</em>I, <em>Hind</em>IIIA, <em>Hind</em>IIIG, <em>Rsa</em>I and <em>Hinf</em>I, located within the β-globin gene cluster region in four subpopulations of Iran. <br/><em>Materials and Methods:</em>A total of 552 blood samples taken from the Iranian subpopulations including Isfahan, Chaharmahal-O-Bakhtiari, Khuzestan and Hormozgan were genotyped using PCR-RFLP and sequencing. The allele frequency, the expected and observed heterozygosity, and Shannon's information index (I) of these markers were calculated. <br/><em>Results:</em>Distribution of the allele frequencies for <em>Xmn</em>I, <em>Hind</em>IIIA, <em>Hind</em>IIIG, <em>Rsa</em>I and <em>Hinf</em>I polymorphic markers did not differ significantly among the subpopulations examined. Overall observed heterozygosity ranged from 0.1706 for <em>Hind</em>IIIA to 0.4484 for <em>Rsa</em>I. The Shannon index was <em>Conclusion:</em>The results suggested that genotyping of these markers is not informative enough once used as single markers for prenatal diagnosis and carrier detection of β-thalassemia in the Iranian population. However, haplotyping of these markers may provide more useful data in linkage analysis and prenatal diagnosis as well as carrier detections for β-thalassemia in Iranians.
β-globin gene cluster region,β-thalassemia,Iranian Population,Polymorphic markers
https://ijbms.mums.ac.ir/article_4531.html
https://ijbms.mums.ac.ir/article_4531_4dc129142d65537b4bf8ac3f7fd4764a.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
18
6
2015
06
01
Development of RNA aptamers as molecular probes for HER2+ breast cancer study using cell-SELEX
576
586
EN
Seyedeh Alia
Moosavian
Biotechnology Research Center, Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
moosaviana871@mums.ac.ir
Mahmoud Reza
Jaafari
0000-0003-3908-6828
Biotechnology Research Center, Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
jafarimr@mums.ac.ir
Seyed Mohammad
Taghdisi
0000-0001-9836-2189
Targeted Drug Delivery Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
taghdisihm@mums.ac.ir
Fatemeh
Mosaffa
Biotechnology Research Center, Pharmacy School, Mashhad University of Medical Sciences, Mashhad, Iran
Ali
Badiee
Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Khalil
Abnous
0000-0001-6314-0164
Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
abnouskh@mums.ac.ir
10.22038/ijbms.2015.4532
<em>Objective(s)</em>: Development of molecules that specifically recognize cancer cells is one of the major areas in cancer research. Human epidermal growth factor receptor 2 (HER2) is specifically expressed on the surface of breast cancer cells. HER2 is associated with an aggressive phenotype and poor prognosis. In this study we aimed to isolate RNA aptamers that specifically bind to HER2 overexpressing TUBO cell line. <br/><em>Materials and Methods:</em> Panel of aptamers was selected using cell-based systematic evolution of ligands by exponential enrichment (cell-SELEX). <br/><em>Results:</em> Binding studies showed that selected aptamers can identify TUBO cell line with high affinity and selectivity. Our preliminary investigation of the target of aptamers suggested that aptamers bind with HER2 proteins on the surface of TUBO cells. <br/><em>Conclusion:</em> We believe the selected aptamers could be useful ligands for targeted breast cancer therapy.
Breast Cancer,Cell-SELEX,RNA aptamer,TUBO cell line
https://ijbms.mums.ac.ir/article_4532.html
https://ijbms.mums.ac.ir/article_4532_d81da845f0f1a0770171012312498abf.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
18
6
2015
06
01
Effects of treadmill exercise training on cerebellar estrogen and estrogen receptors, serum estrogen, and motor coordination performance of ovariectomized rats
587
592
EN
Saidah
Rauf
Department of Physiology, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
ayi_rauf@yahoo.co.id
Sri Kadarsih
Soejono
Department of Physiology, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
Ginus
Partadiredja
0000-0003-0395-4240
Department of Physiology, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
gpartadiredja@yahoo.com
10.22038/ijbms.2015.4533
<em>Objective(s)</em>: The present study aims at examining the motor coordination performance, serum and cerebellar estrogen, as well as ERβ levels, of ovariectomized rats (as menopausal model) following regular exercise. <br/><em>Materials and Methods:</em>Ten <em>female </em>Sprague Dawley ratsaged 12 weeks old were randomly divided into two groups; all of which underwent ovariectomy. The first group was treated with regular exercise of moderate intensity, in which the rats were trained to run on a treadmill for 60 min per day for 12 weeks. The second group served as control. Rotarod test was carried out before and after exercise treatment. All rats were euthanized thereafter, and blood and cerebellums of the rats were collected. The serum and cerebellar estrogen as well as cerebellar ERβ levels were measured using ELISA assays. <br/><em>Results: </em>The number of falls in the rotarod task of the exercise group was significantly lower than that of control group. The cerebellar estrogen level of the exercise group was significantly higher than that of control group. Accordingly, there was a significantly negative correlation between the number of falls and cerebellar estrogen level in the exercise group. <br/><em>Conclusion:</em>The present study shows that a lengthy period of regular exercise improves the cerebellar estrogen level and motor coordination performance in ovariectomized rats.
Cerebellum,Estrogen,Menopause Ovariectomy,Rotarod,Training
https://ijbms.mums.ac.ir/article_4533.html
https://ijbms.mums.ac.ir/article_4533_d647870a24fdda2ba000ad54f05d7e2f.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
18
6
2015
06
01
Association study of four polymorphisms in the interleukin-7 receptor alpha gene with multiple sclerosis in Eastern Iran
593
598
EN
Mehrdad
Sadeghi Haj
Health Research Institute, Infectious and Tropical Disease Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
mehrdad.sadeghihaj@gmail.com
Abbas
Nikravesh
Department of Medical Biotechnology & Molecular Sciences, Faculty of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran
nikraveshabbas2@gmail.com
Majid
Pahlevan Kakhki
Department of Basic Sciences, Gonabad University of Medical Sciences, Gonabad, Iran
Nahid
Rakhshi
Department of Medical Biotechnology & Molecular Sciences, Faculty of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran
nahid.rakhsh52@gmail.com
10.22038/ijbms.2015.4536
<em>Objective(s):</em> Multiple sclerosis (MS) is an autoimmune demyelinating disease of the central nervous system (CNS) with unknown etiology. Various genetics and environmental factors contribute to the pathogenesis of the disease. The interleukin-7 receptor alpha chain (IL-7Ra) was identified as the first non-major histocompatibility complex (non-MHC) MS susceptibility locus. In this study we are trying to find the association of IL-7Ragene polymorphisms with MS susceptibility in Eastern Iran. <br/><em>Materials and Methods:</em>A case-control study was performed in two provinces Sistan & Baluchistan and Khorasan with 219 patients and 258 unrelated matched healthy controls, using PCR-RFLP method for four single nucleotide polymorphisms (SNPs) rs7718919, rs11567685, rs11567686 and rs6897932 of IL-7Ra gene. <br/><em>Results:</em>We found a tendency toward association with genotyping analyses in SNP rs7718919 (<em>P</em>=0.048, OR=4.344, and 95% CI=0.892-21.146); also genotype and allele frequency in gender and MS subtype stratification were shown to have significant association with MS. Analysis of two provinces separately showed a significant difference in results of the allele and genotype frequencies. Moreover, haplotyping analysis showed that (GTGC) has an association only in the male secondary-progressive multiple sclerosis (SPMS) patients in comparison to the healthy controls (<em>P</em>=0.043, OR=0.413, and 95% CI=0.179–0.955). <br/><em>Conclusion:</em>IL7-Ra could be a susceptible gene to MS within the Eastern Iran population especially after MS and gender stratification.
Association study,Haplotype,Interleukin-7 receptor alpha Multiple sclerosis,SNPs
https://ijbms.mums.ac.ir/article_4536.html
https://ijbms.mums.ac.ir/article_4536_033e42d137326e2f4f94c69a4c578d0a.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
18
6
2015
06
01
Inhibition of janus kinase 2 by compound AG490 suppresses the proliferation of MDA-MB-231 cells via up-regulating SARI (suppressor of AP-1, regulated by IFN)
599
603
EN
Yan-xia
Zhang
Department of Radiation Oncology, Shandong Cancer Hospital, Shandong University, Jinan, Shandong, China
Li
Yan
Department of Radiation Oncology, Linyi People’s Hospital, Linyi, Shandong, China
2542934639@qq.com
Guang-yu
Liu
Department of Traumatology, Linyi People’s Hospital, Linyi, Shandong, China
Wen-jun
Chen
Department of Radiation Oncology, Linyi People’s Hospital, Linyi, Shandong, China
Wei-hong
Gong
Department of Radiation Oncology, Linyi People’s Hospital, Linyi, Shandong, China
Jin-ming
Yu
Department of Radiation Oncology, Shandong Cancer Hospital, Shandong University, Jinan, Shandong, China
jinmy86@163.com
10.22038/ijbms.2015.4538
<em>Objective(s): </em>The Janus kinase-signal transducers and activators of transcription signaling pathway (JAK/STAT pathway) play an important role in proliferation of breast cancer cells. Previous data showed that inhibition of STAT3 suppresses the growth of breast cancer cells, but the associated mechanisms are not well understood. This study aims to investigate the effect and associated mechanisms of JAK/STAT pathway inhibitor AG490 on proliferation and suppression of breast cancer cells. <br/><em>Materials and Methods:</em> CCK-8 assay and trypan blue exclusion assay were used to investigate the cytotoxicity of AG490 to MDA-MB-231 cells. Real-time PCR was used to detect the mRNA level of SARI (suppressor of AP-1, regulated by IFN). Western blot was used to analyze the protein levels of SARI, phospho-STAT3 and total STAT3. Luciferase reporter assay was adopted to explore the mechanism of SARI mRNA upregulation. <br/><em>Results:</em> AG490 suppressed the proliferation of MDA-MB-231 cells in a dose-dependent manner. AG490 significantly up-regulated the mRNA and protein levels of SARI in MDA-MB-231 cells. Knockdown of SARI obviously attenuated AG490-induced growth suppression effect in MDA-MB-231 cells. Furthermore, AG490 dramatically enhanced the transcription activity of SARI promoter. But the transcription activity of truncated SARI promoter, which does not contain STAT3 binding site, cannot be activated by AG490 treatment. <br/><em>Conclusion: </em>We demonstrate in this study that AG490 suppresses the proliferation of MDA-MB-231 cells through transcriptional activation of SARI.
AG490,MDA-MB-231,Proliferation,SARI,STAT3
https://ijbms.mums.ac.ir/article_4538.html
https://ijbms.mums.ac.ir/article_4538_c5da55d104173101fe17c446f941584f.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
18
6
2015
06
01
The preventive effects of dexmedetomidine against intestinal ischemia-reperfusion injury in Wistar rats
604
609
EN
Xue-kang
Zhang
Department of Anesthesiology, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, China
zxuekang@gmail.com
Xiao-ping
Zhou
Grade 2012 of Medical Department of Graduate School, Nanchang University, Nanchang, Jiangxi 330006, China
yusong142@gmail.com
Qin
Zhang
Department of Anesthesiology, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, China
jhong0222@gmail.com
Feng
Zhu
Intensive Care Unit (ICU), First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, China
yunfu22@gmail.com
10.22038/ijbms.2015.4539
<em>Objective(s):</em> Intestinal ischemia-reperfusion is a major problem, which may lead to multiorgan failure and death. The aim of this study was to evaluate the protective effects of dexmedetomidine on cell proliferation, antioxidant system, cell death, and structural integrity in intestinal injury induced by ischemia-reperfusion in rats. <br/><em>Materials and Methods:</em> Animals were randomized into three groups: group A, sham-operated or control; group B, intestinal ischemia/reperfusion (IR); and group C, intestinal IR pretreated with 50 μg of dexmedetomidine. Intestine tissue was collected from all rats 30 min after desufflation, and fresh frozen for histological and biochemical evaluation. <br/><em>Results:</em> The intestinal tissue of group B rats showed a significant decrease in the antioxidant enzyme activities. However, these enzyme activities were improved by the administration of dexmedetomidine. Inhibiting the protein expression of MCP<sub>7</sub>, PAR<sub>2</sub>, P-JAK, P-STAT<sub>1</sub>, and P-STAT<sub>3</sub> proved the protective effect of dexmedetomidine. The immunohistochemical staining revealed its protective effect by maintaining the normal structural integrity, less caspase-3 immuno reactivity, and increased cell proliferation count in the intestinal tissues. <br/><em>Conclusions: </em>Intraperitoneal injection of dexmedetomidine significantly protected intestine IR injury in rats by inhibiting the inflammatory response, intestinal epithelial apoptosis, and maintaining structural integrity of intestinal cells.
Dexmedetomidine Inflammatory–response,Intestinal injury,Ischemia-reperfusion
https://ijbms.mums.ac.ir/article_4539.html
https://ijbms.mums.ac.ir/article_4539_a420273f729430dba3cfa80aee4a05c0.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
18
6
2015
06
01
Receptor for advanced glycation end products involved in circulating endothelial cells release from human coronary endothelial cells induced by C-reactive protein
610
615
EN
Shulai
Zhou
Children’s Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310003 China
z20976855@126.com
Lichao
Gao
Children’s Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310003 China
gaolichao306@yahoo.com.cn
Fangqi
Gong
Children’s Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310003 China
gongfangqi@zju.edu.cn
Xiaoyang
Chen
Children’s Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310003 China
yangmeme1026@163.com
10.22038/ijbms.2015.4541
<em>Objective(s)</em>: This study was designed to investigate the effect of receptor for advanced glycation end products (RAGE), S100A12 and C-reactive protein (CRP) on the release of circulating endothelial cells (CECs) from human coronary artery endothelial cells (HCAECs).
<em>Materials and Methods</em>: HCAECs were cultured in increasing concentration of CRP (0, 12.5, 25, 50μg/ml) or S100A12 protein (0, 4, 10, 25μg/ml) for 24 hr. CECs were measured by flow cytometry. Small interfering RNA (siRNA) was designed to decrease RAGE level. Fluorescence microscopy and real-time quantitative polymerase chain reaction were used to assess the efficiency of siRNA silencing RAGE. The release of CECs from HCAECs was further evaluated by flow cytometry.
<em>Results:</em> CRP caused a significant increase in the release of CECs from HCAECs. The number of CECs increased by about 2-fold in 25 μg/ml CRP-treated group compared to the control group (12.22% compared to 6.82%, <em>P</em>=0.032). But S100A12 failed to increase the release of CECs from HCAECs. Blockade of RAGE by siRNA significantly decreased the release of CECs induced by CRP (13.22% of CRP group compared to 8.77% of CRP+siRNA group, <em>P</em>=0.017).
<em>Conclusion:</em>RAGE is involved in the release of CECs induced by CRP, and the effect can be attenuated by silencing RAGE. RAGE may play an important role in endothelial dysfunction in cardiovascular disease. Inhibition of RAGE may be a therapeutic target for coronary artery lesions in Kawasaki disease.
CECs,CRP,HCAECs,RAGE,S100A12
https://ijbms.mums.ac.ir/article_4541.html
https://ijbms.mums.ac.ir/article_4541_762e9ac1877f7d71d4e627b441719268.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
18
6
2015
06
01
Evaluation of cutaneous wound healing activity of Malva sylvestris aqueous extract in BALB/c mice
616
622
EN
Mohammad
Afshar
Department of Anatomy, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran
salamafshar-md@yahoo.com
Behdad
Ravarian
Department of Medical Science, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran
ravibood@gmail.com
Mahmoud
Zardast
Department of Pathology, Birjand University of Medical Sciences, Birjand, Iran
dr.zardast@yahoo.com
Seyed Adel
Moallem
Medical Toxicology Research Centre, Mashhad University of Medical Sciences, Mashhad, Iran
samoallem@yahoo.com
Mohammad
Hasanpour Fard
Department of Pharmacology, Birjand University of Medical Sciences, Birjand, Iran
Masoomeh
Valavi
Department of Animal Sciences, Birjand University, Birjand, Iran
m_valavi27@yahoo.com
10.22038/ijbms.2015.4542
<em>Objective(s):</em> The aim of this study was to evaluate the effects of <em>Malva sylvestris</em> aqueous extract on cutaneous wound healing in BALB/c mice. <br/><em>Materials and Methods:</em> Twenty seven male BALB/c mice (2.5 months of age) were used. A cut wound (superficial fascia depth) was made locally. The mice were then divided into three groups: the first, second and third groups received topical administration of <em>M. sylvestris</em> 1% aqueous extract, silver sulfadiazine topical cream or cold cream (positive and negative control groups), respectively. On days 4, 7 and 10 excisional biopsies were performed and wound healing was evaluated histopathologically. The data were analyzed by the ANOVA and Tukey statistical tests. <br/><em>Results:</em> On days 4 and 7, the numbers of inflammatory cells in the silver sulfadiazine and <em>M. sylvestris</em>-treated groups were significantly lower than the control group and keratinization at the edges of the wound in both groups was significantly higher than the control group. On the tenth day of the study, the <em>Malva</em>-treated mice showed better healing features and less fibrosis and scar formation, and also fewer hair follicles were damaged in this group. On the tenth day of the study, the numbers of inflammatory cells in <em>M. sylvestris</em> and silver sulfadiazine-treated groups were significantly lower than the control group. <br/><em>Conclusion:</em> The present study supports the beneficial effects of <em>M. sylvestris</em> on the wound healing process and suggests a potential clinical application.
Malva sylvestris,Mice,Skin,Wound healing
https://ijbms.mums.ac.ir/article_4542.html
https://ijbms.mums.ac.ir/article_4542_08ba3a43575e03b6a4b728c5b4d929ca.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
18
6
2015
06
01
Association between mutations in gyrA and parC genes of Acinetobacter baumannii clinical isolates and ciprofloxacin resistance
623
626
EN
Abdollah
Ardebili
Department of Microbiology, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
ardebili2001@yahoo.com
Abdolaziz
Rastegar Lari
Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
azizlari@gmail.com
Maryam
Beheshti
Department of Microbiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
maryam.beheshti2012@gmail.com
Elnaz
Rastegar Lari
Department of Science and Environment, Faculty of Science, Technology and Communication, Luxembourg University and Faculty of Science and Environment University of Liege, Liege, Belgium
rastegarlari@gmail.com
10.22038/ijbms.2015.4543
<em>Objective(s)</em>: We investigated the contribution of <em>gyrA</em> and <em>parC</em> mutational mechanism in decreased ciprofloxacin susceptibility of <em>Acinetobacter baumannii</em> isolated from burn wound infections.
<em>Materials and Methods:</em> Ciprofloxacin susceptibility of 50 <em>A. baumannii</em> isolates was evaluated by disk diffusion and agar dilution methods. PCR and sequencing were performed for detection of mutation in <em>gyrA </em>and <em>parC</em> genes.
<em>Results: </em>The 44 and 4 isolates of <em>A. baumannii </em>exhibited full and intermediate-resistant to ciprofloxacin, respectively. Overall, the 42 isolates with double mutations of <em>gyrA</em> and <em>parC</em> genes showed a higher level of ciprofloxacin resistance than the 3 isolates with single mutations of <em>gyrA</em> or <em>parC</em>.
<em>Conclusion:</em> Simultaneous mutations in <em>gyrA</em> and <em>parC</em> genes are expected to play a major role in high-level fluoroquinolone resistance in<em> A. baumannii</em>; albeit a single mutation in DNA topoisomerase IV could occasionally be associated with intermediate-resistance to these antimicrobials.
Acinetobacter baumannii,Burn,Ciprofloxacin resistance,gyrA,parC
https://ijbms.mums.ac.ir/article_4543.html
https://ijbms.mums.ac.ir/article_4543_c18731cedf898e076c7b5be1471c6fa9.pdf