Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
12
2
2009
04
01
Synthesis and Purification of 7-Prenyloxycoumarins and Herniarin as Bioactive Natural Coumarins
63
69
EN
Mahdi
Askari
Department of Pharmacognosy, Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Amirhossein
Sahebkar
Department of Pharmacognosy, Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Mehrdad
Iranshahi
0000-0002-3018-5750
Department of Pharmacognosy, Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
iranshahim@mums.ac.ir
10.22038/ijbms.2009.5145
Objective(s)
7-prenyloxycoumarins including 7-isopentenyloxycoumarin, auraptene and umbelliprenin, and herniarin have been widely recognized as bioactive coumarins. This paper presents the ways to synthesis these compounds.
Materials and Methods
7-prenyloxycoumarins were synthesized by reaction between 7-hydroxycoumarin (' M) and relevant prenyl bromides (1.5 M) in acetone at room temperature. The reaction was carried out in the presence of DBU (1, 8-diazabicyclo [5.4.0] undec-7-ene) (2 M). After 24 hr, the mixture was concentrated under reduced pressure. The compounds were purified by column chromatography.
Results
Three bioactive 7-prenyloxycoumarins, namely, umbelliprenin, auraptene and 7-isopentenyloxycoumarin, together with herniarin were synthesized from 7-hydroxycoumarin under alkaline conditions (DBU) and then purified by column chromatography. The structures of the products were characterized by NMR spectroscopic method including <sup>1</sup>H- and <sup>13</sup>C-NMR experiments.
Conclusion
The method of synthesis for 7-prenyloxycoumarins and herniarin which is presented here has not been reported yet. Moreover, for the first time, umbelliprenin was chemically prepared in this work.
Auraptene,Herniarin,7- Isopentenyloxycoumarin,7-Prenyloxycoumarins,Synthesis,Umbelliprenin
https://ijbms.mums.ac.ir/article_5145.html
https://ijbms.mums.ac.ir/article_5145_2ad17899ddd6c0e50591f7bf6a3ac8d9.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
12
2
2009
04
01
Ex vivo Expansion and Differentiation of Mesenchymal Stem Cells from Goat Bone Marrow
70
79
EN
Mohamadreza
Baghaban Eslaminejad
0000-0002-1036-0072
Stem Cell Department, Cell Science Research Centre, Royan Institute, ACECR, Tehran, Iran
bagesla@yahoo.com
Hamid
Nazarian
Stem Cell Department, Cell Science Research Centre, Royan Institute, ACECR, Tehran, Iran
Fahimeh
Falahi
Stem Cell Department, Cell Science Research Centre, Royan Institute, ACECR, Tehran, Iran
Leila
Taghiyar
Stem Cell Department, Cell Science Research Centre, Royan Institute, ACECR, Tehran, Iran
Mohamad Taghi
Daneshzadeh
Royan Animal Facility, Karaj, Iran
10.22038/ijbms.2009.5146
Objective(s)
Mesenchymal stem cells (MSCs) from large animals as goat which is genetically more closely related to human have rarely been gained attentions. The present study tried to isolate and characterize MSCs from goat bone marrow.
Materials and Methods
Fibroblastic cells appeared in goat marrow cell culture were expanded through several subcultures. Passaged-3 cells were then differentiated among the osteogenic, adipogenic and chondrogenic cell lineages to determine their MSC nature. Differentiations were determined by RT-PCR analysis of related gene expression. To identify the best culture conditions for propagation, passage-3 cells were plated either at varying cell densities or different fetal bovine serum (FBS) concentrations for a week, at the end of which the cultures were statistically compared with respect to the cell proliferation. In this study, we also determined goat MSC population doubling time (PDT) as the index of their in vitro expansion rate.
Results
Passage-3 fibroblastic cells tended to differentiate into skeletal cell lineages. This was evident in both specific staining as well as the specific gene expression profile. Moreover, there appeared to be more expansion when the cultures were initiated at 100 cells/cm<sup><sup>[1]</sup></sup> in a medium supplemented with 15% FBS. A relatively short PDT (24.94±2.67 hr) was a reflection of the goat MSC rapid rate of expansion.
Conclusion
Taken together, fibroblastic cells developed at goat marrow cell culture are able to differentiate into skeletal cell lineages. They undergo extensive proliferation when being plated at low cell density in 15% FBS concentration.
Adipogenesis,Bovine serum,Cell seeding density,Chondrogenesis,Goat mesenchymal stem cells,Osteogenesis
https://ijbms.mums.ac.ir/article_5146.html
https://ijbms.mums.ac.ir/article_5146_a22a60e945fb28d9b6e961c0a2da1f15.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
12
2
2009
04
01
The Impact of Gender on the Inflammatory Parameters and Angiogenesis in the Rat Air Pouch Model of Inflammation
80
85
EN
Tahereh
Eteraf Oskouei
School of Health and Nutrition, Tabriz University of Medical Sciences, Tabriz, Iran
eteraf_t@yahoo.com
Nasrin
Maleki-dizaji
Department of Pharmacology and Toxicology, School of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
Moslem
Najafi
Department of Pharmacology and Toxicology, School of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
najafimoslem@yahoo.com
10.22038/ijbms.2009.5148
Objective(s)
Air pouch is a well-established inflammatory model in which fluid extravasations; leukocyte migration, angiogenesis and other parameters involved in the inflammatory response can be measured. In this study, the influence of gender on inflammatory parameters has been examined in the air pouch model.
Materials and Methods
To induce air pouch, adult male and female Wistar rats were anesthetized, then 20 ml and 10 ml of sterile air were injected subcutaneously on the back on day 0 and day 3, respectively. On day 6, inflammation was induced by injection of 1 ml of carrageenan 1% into pouches. After 6 and 72 hr, the rats were sacrificed, pouch fluid was collected in order to determine exudates volume and the accumulated cells were counted using a hemocytometer. Pouches were dissected out and weighed. Angiogenesis of granulomatous tissue was assayed using hemoglobin kit.
Results
Analysis of our data demonstrated a sexually dimorphic pattern in inflammation parameters both in acute and chronic forms (P<0.05). The value of angiogenesis in the air pouch model in male rat was higher than that female rats (P<0.001).
Conclusion
The degree of inflammation and angiogenesis induced in Wistar rat air pouch model is gender-dependent, suggesting that gender may be a key consideration in the design of inflammation experiments.
Air Pouch,Angiogenesis,Gender,Inflammation
https://ijbms.mums.ac.ir/article_5148.html
https://ijbms.mums.ac.ir/article_5148_3eedd572dc4535d892fc8b57f94b4bc9.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
12
2
2009
04
01
Effect of Cell Wall, Cytoplasmic Fraction and Killed-Candida albicans on Nitric Oxide Production by Peritoneal Macrophages from BALB/c Mice
86
92
EN
Monire
Hajimoradi
Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
hajimoradi@modares.ac.ir
Saeed
Daneshmandi
Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
daneshmandi2006@yahoo.com
Maryam
Roudbary
Department of Mycology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
m_roudbary@yahoo.com
Shahla
Roudbar Mohammadi
Department of Mycology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Zuhair Mohammad
Hassan
0000-0001-9197-1416
Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
hasan_zm@modares.ac.ir
Rozita
Heidari
Department of Mycology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Hasan
Namdar Ahmadabad
Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
namdar.h@modares.ac.ir
10.22038/ijbms.2009.5147
Objective(s)
The fractions of Candida albicans have been used as an immunomodulator. The present work assessed the effect of different fractions of C. albicans on nitric oxide (NO) production by mice peritoneal macrophages. Materials and Methods
Cell wall and cytoplasmic fractions of C. albicans ATCC 10321 strain were extracted. Mice peritoneal macrophages were purified and cultured. Different concentrations of both fractions and also killed C. albicans cells were used for macrophages stimulation and evaluation of NO production. NO amount was detected in culture supernatants of macrophages by Griess reagent. Also, MTT assay was performed to assess the viability of macrophages.
Results
The results elucidated that suppressive effect of cell wall proteins on NO release was significant at the dose of 100 pg/ml (P=0.01), while cytoplasmic fraction increased NO amount at the dose of 1 pg/ml compared to the control group (P=0.003). Augmentation of NO production was statistically significant at 200 killed C. albicans per well (P=0.006).
Conclusion
According to our findings, cytoplasmic fractions and killed C. albicans have a positive effect on NO production by peritoneal macrophages, while cell wall fractions did not. Therefore, it is proposed that C. albicans fractions can be studied more as inflammation modulators.
Candida albicans,Macrophages,Nitric oxide
https://ijbms.mums.ac.ir/article_5147.html
https://ijbms.mums.ac.ir/article_5147_2f137df2c18670fe484382bebfa25c80.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
12
2
2009
04
01
Comparison of Prooxidant-antioxidant Balance Method with Crocin Method for Determination of Total Prooxidant-antioxidant Capacity
93
99
EN
Daryoush
Hamidi Alamdari
Department of Biological Chemistry, Medical School, Aristotle University of Thessaloniki, Thessaloniki, Greece
dhamidialam@yahoo.com
Stella
A. Ordoudi
Department of Chemistry, Laboratory of Food Chemistry and Technology, Aristotle University of Thessaloniki,
Thessaloniki, Greece
Nikolaos
Nenadis
Department of Chemistry, Laboratory of Food Chemistry and Technology, Aristotle University of Thessaloniki,
Thessaloniki, Greece
Maria
Z. Tsimidou
Department of Chemistry, Laboratory of Food Chemistry and Technology, Aristotle University of Thessaloniki,
Thessaloniki, Greece
George
Koliakos
Department of Biological Chemistry, Medical School, Aristotle University of Thessaloniki, Thessaloniki, Greece
koliakos@med.auth.gr
Seyyed Mohmmad Reza
Parizadeh
Department of Nutrition and Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Mohammad
Safarian
Department of Nutrition and Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Maryam
Sabery Karimian
Department of Nutrition and Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
B. Fatemeh
Nobakht M. Gh
Department of Nutrition and Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
10.22038/ijbms.2009.5149
Objective(s)
Comparison of prooxidant-antioxidant balance (PAB) assay with crocin assay.
Materials and Methods
Twenty eight serum samples were chosen, PAB and the total antioxidant capacity were measured by PAB assay and crocin, respectively, and the correlation of both assays, along with their correlation with other clinical and biochemical parameters, were determined.
Results
A significant negative correlation was established between PAB assay and crocin assay. Also a significant negative correlation was established between PAB and uric acid and creatinine.
Conclusion
The results showed that by increasing the total antioxidant capacity, which is showed by crocin, the PAB shifts in favor of antioxidants, which is showed by PAB assay. Now, it could be considered that the PAB, along with other risk factors, might help in the prediction of the risk for cardiovascular events; and further research could clarify whether by application of PAB assay and appropriate interventions for correcting oxidative stress, progression of the cardiovascular disease could be reduced.
Crocin,Oxidative stress,Prooxidant-antioxidant balance assay
https://ijbms.mums.ac.ir/article_5149.html
https://ijbms.mums.ac.ir/article_5149_8c5b21447badc1a482bf1d5b48304958.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
12
2
2009
04
01
Evaluation of Relation between IL-4 and IFN-y Polymorphisms and Type 2 Diabetes
100
104
EN
2Mohammad
Kazemi Arababadi
Department of Hematology and Immunology, School of Medicine, Rafsanjan University of Medical Sciences,Rafsanjan, Iran
kazemi24@yahoo.com
Ali Akbar
Pourfathollah
Department, of Immunology, School of Medicine, Tarbiat Modares University, Tehran, Iran
pourfa@moadres.ac.ir
Saeed
Daneshmandi
Department, of Immunology, School of Medicine, Tarbiat Modares University, Tehran, Iran
daneshmandi2006@yahoo.com
Gholamhossein
Hassanshahi
Department of Hematology and Immunology, School of Medicine, Rafsanjan University of Medical Sciences,Rafsanjan, Iran
ghhassanshahi@rums.ac.ir
Ebrahim
Rezazadeh Zrandi
Department of Hematology and Immunology, School of Medicine, Rafsanjan University of Medical Sciences,Rafsanjan, Iran
Ali
Shamsizadeh
0000-0001-8329-9156
Molecular-Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
alishamsy@gmail.com
Majid
Asiabanha Rezaei
Department of Biochemistry, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
Somayeh
Eigder
Department of Biochemistry, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
10.22038/ijbms.2009.5150
Objective(s)
Although, type 2 diabetes is the most frequent type of diabetes, its main cause is yet to be clarified. Several environmental and genetic parameters are believed to be involved in diabetes. It has also been established that cytokines play key roles in pathogenesis of diabetes. Expression of cytokines is different from person to person and in different societies. Several studies showed that polymorphisms of +874 of interferon-gamma (IFN-y) and -590 of interleukin-4 (IL-4) are associated with the regulation of expression of these genes. This study was aimed to find polymorphisms of these regions in type 2 diabetes patients.
Materials and Methods
In this experimental study peripheral blood samples were collected from 160 type 2 diabetic patients and 160 healthy controls. DNA was extracted by salting out method. Polymorphisms of +874 of IFN-y and -590 of IL-4 were analyzed by ARMS-PCR and RFLP-PCR.
Results
Our findings indicated that TT genotype of IFN-y was increased in type 2 diabetic patients compared to the control but difference was not significant. Our results didn’t show any significant difference between IL-4 genotype in diabetic and healthy controls either.
Conclusion
Our results suggested that TT genotype of IFN-y can be associated with diabetes. This association can be described by the fact that over expression of IFN-y shifts immune system to Th1; therefore, pancreatic cells can be miscarried by immune cells.
Interferon-gamma,Interleukin-4,Polymorphism,Type 2 diabetes
https://ijbms.mums.ac.ir/article_5150.html
https://ijbms.mums.ac.ir/article_5150_2c8e8a6718f88bbb9e9f66b536eb2d94.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
12
2
2009
04
01
Antibacterial Activity of Twenty Iranian Plant Extracts Against Clinical Isolates of Helicobacter pylori
105
111
EN
Farahnaz
Nariman
Department of Biology and Microbiology, Faculty of Sciences, Alzahra University, Tehran, Iran
Fereshteh
Eftekhar
0000-0002-2441-2582
Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, G.C., Tehran, Iran
f-eftekhar@sbu.ac.ir
Zohreh
Habibi
Department of Chemistry, Faculty of Sciences, Shahid Beheshti University, G.C., Tehran, Iran
Sadegh
Massarrat
Digestive Diseases Research Center, Faculty of Medical Sciences, Tehran University, Tehran, Iran
Reza
Malekzadeh
Digestive Diseases Research Center, Faculty of Medical Sciences, Tehran University, Tehran, Iran
malekzadeh@gmail.com
10.22038/ijbms.2009.5151
Objective(s)
Due to increasing emergence of drug-resistance in Helicobacter pylori isolates, traditional plants are potentially valuable sources of novel anti-H. pylori agents. In this research, anti-H. pylori activity of the organic extracts of twenty native Iranian plants was determined against ten clinical isolates of H. pylori. Materials and Methods
Disc diffusion was used to determine the biological activity of 20 plant extracts as well as 8 antibiotics commonly used to treat H. pylori infections. Minimum inhibitory concentrations were also measured by tube and agar dilution methods for the biologically active plant extracts.
Results
Of the twenty plant extracts analyzed, sixteen exhibited good anti-H. pylori activity, using disc diffusion. The ten most active extracts were Carum bulbocastanum, Carum carvi, Mentha longifolia, Saliva limbata, Saliva sclarea, Ziziphora clinopodioides, Thymus caramanicus, Glycyrrhiza glabra, Xanthium brasilicum and Trachyspermum copticum. Minimum inhibitory concentrations measured for the 10 biologically active plant extracts were within the range of 31.25 to 500 ^g/ml.
Conclusion
Among the ten plant extracts effective against H. pylori clinical isolates, Carum carvi, Xanthium brasilicum and Trachyspermum copticum showed the highest activity.
Anti-Helicobacter pylori,Iranian plants,Organic extracts
https://ijbms.mums.ac.ir/article_5151.html
https://ijbms.mums.ac.ir/article_5151_e2ac8db118f682e5e75d9d43c62d9f87.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
12
2
2009
04
01
Qualitative and Quantitative Analysis of the Effects of Quinazolinones on Internal Organs of Newborn Balb/C Mice
112
120
EN
Maryam
Shams Lahijani
Developmental Biology,Animal Sciences, Faculty of Biological Sciences, Shahid-Beheshti University (SBU),G.C., Tehran, Iran
mslahijani2006@gmail.com
Hoda
Rajabi
Developmental Biology,Animal Sciences, Faculty of Biological Sciences, Shahid-Beheshti University (SBU),G.C., Tehran, Iran
Samar
Etemad
Developmental Biology,Animal Sciences, Faculty of Biological Sciences, Shahid-Beheshti University (SBU),G.C., Tehran, Iran
Mahla
Fadavi Eslam
Developmental Biology,Animal Sciences, Faculty of Biological Sciences, Shahid-Beheshti University (SBU),G.C., Tehran, Iran
10.22038/ijbms.2009.5154
Objective(s)
Quinazolinones are heterocyclic compounds, with biological and pharmacological activities, such as inhibiting some proteins, enzymes and reducing blood lipids.
Materials and Methods
Following previous results of our group, effects of two new derivatives of quinazolinones 9(3)-quinazolinone-2-propyl-2-phenylethyl (QPPE) and 9(3)-quinazolinone-2-ethyl-2-phenylethyl (QEPE) on livers, intestines and kidneys of newborn Balb/C mice were investigated. Pregnant mice were divided into four groups of control, sham, experimental 1, treated with QPPE, and experimental 2, treated with QEPE. Experimental groups received 100 mg/kg body weight (most effective dose) of QPPE and QEPE, sham groups received methyl cellulose 0.05% (the solvent) and control groups received distilled water, intraperitoneally (IP), on day 8 of gestation. Five days after birth, livers, intestines and kidneys were removed, fixed in formalin 10%, stained with hematoxylene and eosin for histological and pathological studies.
Results
Results showed appearance of fatty changes in livers, an increase in diameters of hepatocytes and central veins of livers, and reduction in the lengths of villi of proximal, middle and distal segments of newborn Balb/C mice intestines. Furthermore, there was a diminished diameter of the lumen of the proximal tubules, and average diameter of the lumen of distal tubules which led to an increase in the number of glomeruli cells of newborn Balb/C mice kidneys.
Conclusion
Regarding inflammation in different parts of the kidneys, livers and intestines, our investigations suggest that quinazolinones may have some toxic effects on embryos.
Abnormalities,Intestine,Kidney,Liver,Mice fetuses,Quinazolinones
https://ijbms.mums.ac.ir/article_5154.html
https://ijbms.mums.ac.ir/article_5154_a9b9c267584718142850d3156ceae7b9.pdf
Mashhad University of Medical Sciences
Iranian Journal of Basic Medical Sciences
2008-3866
2008-3874
12
2
2009
04
01
Tissue PCR Diagnosis of Patients Suspicious for Tuberculous Pleurisy
121
125
EN
Mahnaz
Amini
0000-0002-7309-3653
Department of Internal Medicine, Imam Reza Hospital, Mashhad University of Medical Science (MUMS), Mashhad, Iran
aminim@mums.ac.ir
Davood
Attaran
Department of Internal Medicine, Ghaem Hospital, Lung and Tuberculosis Research Center, MUMS, Mashhad, Iran
Kiarash
Ghazvini
Department of Mycobacteriology, Ghaem Hospital, MUMS, Mashhad, Iran
Habibollah
Esmaily
School of Medicine, MUMS, Mashhad, Iran
Mahmood
Bagheri
School of Medicine, MUMS, Mashhad, Iran
10.22038/ijbms.2009.5156
Objective(s) <br/>This study planned to assess the value of PCR IS6110 assay in tissue specimens of needle pleural biopsy in patients suspicious to pleural tuberculosis. <br/>Materials and Methods <br/>Sixty eight patients with lymphocytic exudative pleural effusion underwent pleural biopsy. Tissue samples were sent for pathologic examination and PCR IS6110 assay. The results of PCR reported as positive/ negative and assessed according to the current gold standard pathologic diagnosis. <br/>Results <br/>Twenty nine patients had tuberculous and 12 had malignant pleural involvement, respectively. The remaining 27 samples were reported as non-specific pleurisy. Results of PCR were positive in 35 out of 68 total subjects and in 19 out of 29 TB patients. Sensitivity and specificity of PCR were calculated as 67.9% and 62.5%, respectively. <br/>Conclusion <br/>An acceptable sensitivity and specificity for PCR examination of pleural tissue can serves it as a useful adjunct in undergoing needle pleural biopsy for possibility of tuberculosis.
DNA Primers,Mycobacterium tuberculosis,Pleural,Polymerase chain reaction
https://ijbms.mums.ac.ir/article_5156.html
https://ijbms.mums.ac.ir/article_5156_e5810388cbf2590e2659ee0268dd0f2a.pdf