TY - JOUR ID - 10465 TI - Inhibition of breast cancer metastasis by co-transfection of miR-31/193b-mimics JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Hashemi, Zahra Sadat AU - Forouzandeh Moghadam, mehdi AU - Farokhimanesh, Samila AU - rajabibazl, masuomeh AU - sadroddiny, Esmaeil AD - Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran AD - Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran AD - Department of Clinical Biochemistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran Y1 - 2018 PY - 2018 VL - 21 IS - 4 SP - 427 EP - 433 KW - Breast Cancer KW - Metastasis KW - miR-31 KW - miR-193b KW - RhoA KW - uPA DO - 10.22038/ijbms.2018.26614.6522 N2 - Objective(s): Various studies have been conducted to reduce the metastatic behavior of cancerous cells. In this regard, ectopic expression of anti-metastatic microRNAs by miR-mimic and miR-restoration-based therapies could bring new insights to the field. In the present study, the consequences of co-transfecting breast cancer cell lines with miR-193b and miR-31 were investigated via invasion and migration assays.Materials and Methods: Double stranded oligonucleotide of mature miR-193b-3p and miR-31-5p were cloned into pcDNA 6.2gw/EmGFP plasmid. The resulting plasmids were used for transfection. Real time-PCR was performed to assess the expression of miR-193b and miR-31 as well as Ras homolog gene family member A (RhoA) and urokinase-type plasminogen activator (uPA) as miR targets. Scratch, Transwell migration and Matrigel invasion assays were carried out to assess the extent of migration and invasion of cell lines.Results: The most significant increase in expression of miRs belonged to the single transfection of mimic-miRs in MDA-MB231. Although the co-transfection was not as successful as single transfection in miR expression, it was significantly more effective in inhibition of the cells invasive potential. Conclusion: Although the miR-restoration therapy based on co-transfection of two miRs could be less effective in expression of each miRNA, the resulting decrease in metastatic behavior of the cells is more significant due to collective effect of co-transfection to decrease target gene expression. Our results revealed that employing this sort of combinatorial strategies could lead to more efficient reduction in metastatic behavior. It seems that using this strategy would bring about more successful therapeutic outcomes. UR - https://ijbms.mums.ac.ir/article_10465.html L1 - https://ijbms.mums.ac.ir/article_10465_66763c2c7b92b4a7a96834186ac01bb0.pdf ER -