TY - JOUR ID - 11368 TI - Study of class 1 integrons in multidrug-resistant uropathogenic Escherichia coli isolated from different hospitals in Karachi JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Zeeshan Khan, Fouzia AU - Nawaz, Tehseen AU - Mirani, Zulfiqar AU - Khan, Saeed AU - Raza, Yasir AU - Urooj Kazmi, Shahana AD - Dow University of Health Sciences, Karachi, Pakistan AD - Barrett Hodgson University, Karachi, Pakistan AD - Pakistan Council of Scientific & Industrial Research Laboratories Complex, Karachi, Pakistan AD - Department of Microbiology, University of Karachi, Karachi, Pakistan AD - Dada Bhoy Institute of Higher Education Commission, Karachi, Pakistan Y1 - 2018 PY - 2018 VL - 21 IS - 10 SP - 1079 EP - 1082 KW - Class 1 integrons KW - ESBLs KW - MDR E. coli KW - Multidrug resistance KW - Uropathogenic E. coli DO - 10.22038/ijbms.2018.28807.6966 N2 - Objective(s): Escherichia coli is the key pathogen in the family producing ESBL (extended spectrum β-lactamase) and associated with community-acquired infections. Therefore, this study was planned to determine the antibiotic susceptibility pattern of uropathogenic E. coli, prevalence of the ESBL gene group and class 1 integrons.Materials and Methods: Clinical isolates of uropathogenic E. coli were isolated from different hospitals of Karachi. Antibiotic susceptibility test was performed by Kirby-Bauer Methods. Presence of β– lactamases genes (CTX, TEM, and SHV) and integron 1 were identified by polymerase chain reaction (PCR).Results: Out of 500, 105 isolates were identified as multi-drug resistant (MDR) uropathogenic E. coli. The subject MDR isolates showed the highest resistance to aztreonam, amoxil/ clavulanic acid, ampicillin, cotrimoxazole, ceftriaxone, cefipime, and cefuroxime. Genetic analysis showed that the majority of the MDR E. coli carry CTX M1 (57.1%) followed by TEM (33.3%) and SHV (9.5%). Moreover, 79% of MDR E. coli harbored class 1 integrons, whereas all three conserved genes for class 1 integrons were present in 58% of MDR E. coli. Conclusion: This study is helpful to provide information regarding the antibiotic susceptibility pattern, distribution ESBLs and class 1 integrons among uropathogenic E. coli. UR - https://ijbms.mums.ac.ir/article_11368.html L1 - https://ijbms.mums.ac.ir/article_11368_85f177b94ea4f52d026dd3c7eb3e0a49.pdf ER -