TY - JOUR ID - 12577 TI - The role of microRNA-30a and downstream snail1 on the growth and metastasis of melanoma tumor JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Noori, Jahangir AU - Haghjooy Javanmard, Shaghayegh AU - Sharifi, Mohammadreza AD - Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran AD - Applied Physiology Research Center, Isfahan Cardiovascular Research Institute, Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran AD - Department of Genetics and Molecular Biology- Isfahan University of Medical Sciences, Isfahan, Iran Y1 - 2019 PY - 2019 VL - 22 IS - 5 SP - 534 EP - 540 KW - Epithelial-mesenchymal transition KW - Melanoma KW - Metastasis KW - miR-30a KW - Neoplasm KW - Snail1 DO - 10.22038/ijbms.2019.32317.7745 N2 - Objective(s): Growing evidences have indicated microRNAs as modulators of tumor development and aggression. On the other hand, a phenomenon known as epithelial-mesenchymal transition (EMT) that indicates a transient phase from epithelial-like features to mesenchymal phenotype is a key player in tumor progression.  In this study, we aimed to assess the potential impacts of miR-30a-5p as an inhibitor of melanoma progression and metastasis.Materials and Methods: MiR-30a-5p was transfected into B16-F10 melanoma cells. Then, the B16-F10 cells were injected subcutaneously or intravenously (IV) in to C57BL/6 mice. Then, the mice were euthanized and tumor size, tumor weight, snail1 protein expression and nodules in the lungs were evaluated.Results: The migration of cancerous cells was significantly suppressed in vitro following the ectopic presentation of miR-30a-5p into B16-F10 melanoma cells. Furthermore, the metastatic behavior of the neoplastic cells was further suppressed in a xenograft mouse model of melanoma as observed with limited lung infiltration. We also found that transfected miR-30a-5p into melanoma cells could decrease snail1 and N-cadherin expression. Conclusion: MiR-30a-5p may represent an effective therapeutic target for the management of melanoma and other snail-overexpressing neoplasms. UR - https://ijbms.mums.ac.ir/article_12577.html L1 - https://ijbms.mums.ac.ir/article_12577_c4ce8cda29aa3b35f60fa23187946fa9.pdf ER -