TY - JOUR ID - 13302 TI - Investigation of histopathological and radiological effects of surfactant treatment in an experimental female rat model of lung contusion JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - KESKİN, Yasin AU - BEDEL, Cihan AU - Gökben Beceren, Nesrin AD - Beyhekim State Hospital, Department of Emergency Medicine, Konya, Turkey AD - Health Science University Antalya Training and Research Hospital, Department of Emergency Medicine, Antalya, Turkey AD - Süleyman Demirel University Faculty of Medicine, Department of Emergency Medicine, Isparta, Turkey Y1 - 2019 PY - 2019 VL - 22 IS - 10 SP - 1153 EP - 1157 KW - Blunt chest trauma KW - Chest trauma KW - Contusion KW - Lung contusion KW - Rat KW - Surfactant DO - 10.22038/ijbms.2019.32357.8258 N2 - Objective(s): Pulmonary contusion (PC) is a clinical entity that often accompanies blunt traumas. We aimed to investigate the radiological and histopathological effects of surfactant treatment in an experimental rat model in which lung contusion was formed by blunt thoracic trauma.Materials and Methods: 50 female Sprague-Dawley rats were used. Five groups were formed randomly. In groups 2, 4, and 5 lung contusion was made by the drop-weight method after anesthesia. Intratracheal surfactant was administered in the 4th hr in groups 3 and 4 and in the 24th hr in groups 4 and 5. All rats were sacrificed and their lungs removed at 48 hr after contusion. Alveolar edema, congestion, hemorrhage, destruction, leukocyte infiltration, immune staining were examined histopathologically.Results: When the first thoracic CT scans were evaluated, we observed two rats with rib fractures and four rats with pneumothorax. 4 and 48 hr thoracic CT evaluation contusion and atelectasis showed no statistically significant decrease (P>0.05). After sacrifice of group 2, in macroscopic evaluation, there was a heterogeneous contusion and hemorrhagic appearance in the lungs of rats and less hemorrhagic appearance was observed in Groups 4 and 5 than in Group 2. In comparison of Immunohistopathological findings, surfactant treatment showed a statistically significant decrease in leukocyte infiltration scores (P=0.046). Immunohistopathologically, surfactant group had more staining but only statistically significant when compared to groups 4 and sham. (P=0.036).Conclusion: Surfactant treatment may be of significant benefit in lung contusion secondary to blunt chest trauma, and further prospective evidence of its efficacy in such disorders is needed. UR - https://ijbms.mums.ac.ir/article_13302.html L1 - https://ijbms.mums.ac.ir/article_13302_b638cf75da363853728c64af3f616ff2.pdf ER -