TY - JOUR ID - 13307 TI - Hydrogen sulfide improves vessel formation of the ischemic adductor muscle and wound healing in diabetic db/db mice JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Wang, Guo-Guang AU - Li, Wei AD - Department of Pathophysiology, Wannan Medical College, Wuhu, China Y1 - 2019 PY - 2019 VL - 22 IS - 10 SP - 1192 EP - 1197 KW - Adductor muscle KW - Angiogenesis KW - db/db mice KW - Diabetes KW - Hydrogen sulfide KW - Ischemia DO - 10.22038/ijbms.2019.36551.8709 N2 - Objective(s): It has been demonstrated that hydrogen sulfide plays a vital role in physiological and pathological processes such as regulating inflammation, oxidative stress, and vessel relaxation. The aim of the study was to explore the effect of hydrogen sulfide on angiogenesis in the ischemic adductor muscles of type 2 diabetic db/db mice and ischemic diabetic wound healing.Materials and Methods: The femoral arteries of diabetic db/db mice were isolated and ligated for preparation of ischemic hind limb model. Round incision was made on ischemic and non-ischemic limbs. The wounds were treated with sodium bisulfide (hydrogen sulfide donor). Real-time PCR and Western blotting were used to measure transcription of vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), platelet derived growth factor (PDGF), hypoxia inducible factor-1α (HIF-1α) and endothelial nitric oxide synthase (eNOS) and protein expression of VEGF, VEGF receptor (VEGFR) and PDGF, PDGF receptor (PDGFR), respectively. Angiogenesis and morphological changes in adductor muscles were observed.Results: Hydrogen sulfide significantly increased transcription of VEGF, EGF, PDGF, HIF-1α, eNOS and protein expression of VEGF, PDGF, and phosphorylated VEGFR and PDGFR. Treatment with hydrogen sulfide significantly improved ischemic wound healing and formation of granulation tissue, and increased the number of small vessels in the ischemic adductor muscles.Conclusion: Our data suggested that hydrogen sulfide attenuated injury of ischemic adductor muscle, and promoted the ischemic diabetic wound healing via modulating angiogenesis in type 2 diabetic db/db mice. UR - https://ijbms.mums.ac.ir/article_13307.html L1 - https://ijbms.mums.ac.ir/article_13307_71998b513dc2394fe812426e7c3bc308.pdf ER -