TY - JOUR ID - 13547 TI - Anti-MRSA activity of a bioactive compound produced by a marine Streptomyces and its optimization using statistical experimental design JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Norouzi, Hamed AU - Rabbani khorasgani, Mohamad AU - Danesh, Abolghasem AD - Department of Biology, University of Isfahan, Isfahan, Iran AD - Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran Y1 - 2019 PY - 2019 VL - 22 IS - 9 SP - 1073 EP - 1084 KW - Experimental designs KW - Marine microbiology KW - Methicillin-Resistant Staphylococcus aureus KW - Natural products KW - Fermentation DO - 10.22038/ijbms.2019.33880.8058 N2 - Objective(s): To address the alarming problem of methicillin-resistant Staphylococcus aureus (MRSA), herein, a marine Streptomyces capable of producing an anti-MRSA compound has been studied.Materials and Methods: Strain MN41 was morphologically and physiologically characterized and then, molecularly identified using 16SrRNA analysis. To produce the bioactive compound in large scale, a kind of submerged liquid fermentation was adopted. The antibacterial agent was purified using a silica gel column followed by a semi-preparative HPLC and the isolated metabolite was identified using mass spectrometry, Nuclear magnetic resonance (NMR) and Fourier-transform infrared (FTIR).  Finally, the production process was subjected to a two steps optimization using Plackett-Burman design (PBD) and Response Surface Method (RSM), respectively. In addition, the antitumor activity of the active agent was studied.Results: The purified compound with a molecular weight of 421.2 was identified as a natural pyrrole-derivative. The optimization revealed a significant effect for starch, pH, calcium carbonate and peptone on the production of this anti-MRSA compound and resulted in a 218% increase in the production yield. Conclusion: The isolated pyrrole-derivative showed a remarkable activity against MRSA and also showed some promising anti-tumor activity. UR - https://ijbms.mums.ac.ir/article_13547.html L1 - https://ijbms.mums.ac.ir/article_13547_cc7e2b7743446935d48cec2af97584ef.pdf ER -