TY - JOUR ID - 14053 TI - Morphine and kisspeptin influences on 5-α reductase and aromatase gene expression in adult male rats JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Khazali, Homayoun AU - Mahmoudi, Fariba AD - Department of Animal Science and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran AD - Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Iran Y1 - 2019 PY - 2019 VL - 22 IS - 12 SP - 1462 EP - 1467 KW - Aromatase KW - Hypothalamus KW - Kisspeptin KW - Liver KW - Morphine KW - Testis KW - 5α-reductase DO - 10.22038/ijbms.2019.14053 N2 - Objective(s): Kisspeptin and opioids are important factors for controlling GnRH/LH secretion. In present study, influences of kisspeptin or morphine were investigated on 5α- reductase or aromatase (CYP19) genes expression in the hypothalamus, testis and liver. It aimed to investigate whether kisspeptin pathway may control morphine effects on plasma concentration of testosterone.Materials and Methods: Twenty adult male rats in four groups received saline/saline, kisspeptin (1 nmol)/saline, morphine (5 mg/kg)/saline or kisspeptin/morphine respectively. Mean relative 5α-reductase and aromatase mRNA levels were determined by RT-PCR. Results: Morphine/saline injection increased significantly mean relative mRNA levels of hypothalamic 5α-reductase or aromatase compared to saline/saline. While morphine/saline did not alter mRNA levels of them compared to saline/saline group in the testis and liver. Kisspeptin/saline did not significantly decrease mean relative 5α-reductase or aromatase genes expression compared to saline/saline group in the hypothalamus, testis and liver. Injections of kisspeptin/morphine did not significantly decrease mean relative 5α-reductase or aromatase genes expression compared to morphine/saline group.Conclusion: Up-regulation of hypothalamic 5α-reductase or aromatase mRNA levels may partly induce the inhibitory effects of morphine on GnRH/LH release. Different effects of morphine on aromatase or 5α- reductase genes expression levels in the liver and testis compared to brain may be partly due to different sensitivity or functions of them to morphine used dose. UR - https://ijbms.mums.ac.ir/article_14053.html L1 - https://ijbms.mums.ac.ir/article_14053_868a0b838e5aae2de154fa6a4d049981.pdf ER -