TY - JOUR ID - 15208 TI - Prenatal stress increased γ2 GABAA receptor subunit gene expression in hippocampus and potentiated pentylenetetrazol-induced seizure in rats JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Bagheri, Morteza AU - Saboory, Ehsan AU - Nejatbakhsh, Mehrdad AU - Roshan-Milani, Shiva AU - Derafshpour, Leila AU - Sayyadi, Hojjat AU - Rasmi, Yousef AD - Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, Iran AD - Zanjan Metabolic Diseases Research Center, Zanjan University of Medical Sciences, Zanjan, Iran AD - Department of Physiology, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran AD - Department of Biostatistics , School of Medicine, Urmia University of Medical Sciences, Urmia, Iran Y1 - 2020 PY - 2020 VL - 23 IS - 6 SP - 724 EP - 729 KW - GABAA receptor KW - Infant KW - Prenatal stress KW - Rat KW - Seizure DO - 10.22038/ijbms.2020.39519.9371 N2 - Objective(s): Stress during pregnancy is able to bring extensive effects on neurobehavioral development in offspring. The GABAergic system plays a pivotal role in neuronal excitability, which can be affected by prenatal stress (PS). This study aimed to evaluate impact of the PS on γ2 subunit of gamma-aminobutyric acid A (GABAA) receptor gene expression in the hippocampus and seizure induced by pentylenetetrazol (PTZ) in developing rats.Materials and Methods: In this experimental study, female Wistar rats were exposed to restraint stress during gestation and their offspring were studied on postnatal days 14 and 21 (P14 and P21, respectively) for epileptic behaviors and γ2 GABAA receptor subunit gene expression. Quantitative real-time PCR was used for evaluating the γ2 GABAA receptor subunit gene expression in rat pups. Meanwhile, PTZ was injected into the pups, and seizure behaviors were recorded for 60 min. Results: The results showed that γ2 subunit mRNA expression significantly increased in the hippocampus of the stressed pups. The expression level of γ2 subunit was higher on P21 compared to that on P14 in both groups. Number of seizures with tonic–clonic features increased in pups of stressed group compared to the control group. Prenatal stress significantly caused an increase in the total score of seizure on P21.Conclusion: The effect of PS on seizure susceptibility is age-specific; the increased γ2 subunit level in the hippocampus might be, at least in part, the underlying mechanism for PS-induced augmentation of seizures in immature rats. UR - https://ijbms.mums.ac.ir/article_15208.html L1 - https://ijbms.mums.ac.ir/article_15208_23707ebd7ad9e7ac29a24279fd6c2ea4.pdf ER -