TY - JOUR ID - 15230 TI - Relationship between calcium circulation-related factors and muscle strength in rat sciatic nerve injury model JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Sun, Xiaoming AU - Wang, Wei AU - Dong, Yangyi AU - Wang, Yue AU - Zhang, Meixiang AU - Wang, Zhao AU - Yu, Xiaowei AU - Huang, Jiao AU - Cai, Hongxing AD - Department of Forensic Medicine, Xuzhou Medical University, Xuzhou, Jiangsu, P. R. China Y1 - 2020 PY - 2020 VL - 23 IS - 5 SP - 654 EP - 662 KW - Forensic medicine KW - Muscle atrophy KW - Muscle strength KW - Ryanodine receptor KW - Sciatic nerve DO - 10.22038/ijbms.2020.40915.9695 N2 - Objective(s): The purpose of this study is to investigate the indication function of the calcium circulation-related factors on the damage to muscle strength and contraction function after nerve injury. The target factors include ryanodine receptor (RyR), inositol-1,4,5-triphosphate receptor (IP3R), phospholamban (PLN), cryptocalcitonin (CASQ), ATPase and troponin C (TNNC).Materials and Methods: Sprague-Dawley (SD) rats were randomly divided into sham-operated group (SO), sciatic nerve injury group (SNI) and sciatic nerve disconnection group (SNT). Sciatic nerve function index and stretching test were used to examine the changes to muscle strength; bilateral gastrocnemius muscles were extracted after execution for gastrocnemius wet weight ratio test. HE staining slides and average cross-sectional area of muscle fibers were acquired to analyze the muscle atrophy. The transcription level of the factors was also measured.Results: Sciatic nerve damage in SNI group was significantly higher than that in SO group in the 6 weeks, but there was no significant difference between SNT and SO groups fallowing sciatic nerve damage. Sciatic nerve function in SNT group was worse than that in SNI group. The average cross-sectional area of gastrocnemius muscle fibers in SNI and SNT groups was significantly reduced compared to that in SO group. The transcriptional levels of RyR, PLN, CASQ, ATPase and TNNC in SNI and SNT groups were significantly different from those in SO group.Conclusion: Calcium circulation-related factors could be used as potential indicators for assessment of damages to muscle strength. UR - https://ijbms.mums.ac.ir/article_15230.html L1 - https://ijbms.mums.ac.ir/article_15230_242709ebde649539c0aa9e438c2a9b6a.pdf ER -