TY - JOUR ID - 15704 TI - Protective effect of S-nitrosoglutathione pretreatment on acute lung injury in septic rats JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Wang, Zhou-feng AU - Yang, Yu-min AU - Fan, Heng AD - Department of Respiratory and Critical Care, Ningbo First Hospital Longshan Hospital Medical and Health Group, Ningbo, Zhejiang Province, P.R China AD - Department of Intensive Care Unit, Ningbo First Hospital, Ningbo, Zhejiang Province, P.R China Y1 - 2020 PY - 2020 VL - 23 IS - 8 SP - 1059 EP - 1064 KW - Acute lung injury NF KW - κB Oxidative stress Sepsis S KW - nitrosoglutathione TLR4 DO - 10.22038/ijbms.2020.43590.10240 N2 - Objective(s): To investigate the protective effect of S-nitrosoglutathione (SNG) pretreatment on acute lung injury (ALI) in septic rats. Materials and Methods: We constructed a rat model of sepsis by cecal ligation and perforation (CLP), and randomly divided into Sham, CLP, and CLP+SNG (0.25 and 0.5 mg/kg) groups. We used H&E staining and lung wet/dry ratio to assess the severity of lung injury, detected the levels of protein and cells in bronchoalveolar lavage fluid (BALF) and the levels of TNF-α, IL-1β, TLR4 mRNA, and NF-κB p65 mRNA in the lung tissue, and assessed the levels of glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase in the lung tissue.Results: A rat model of sepsis was successfully constructed by CLP, and pretreatment of SNG significantly increased the survival of septic rats (P<0.001) and decreased the lung tissue injury scores (P<0.001) and lung wet/dry ratio (P<0.01) in a dose-dependent manner. Furtherly, SNG pretreatment significantly reduced the number of total cells, total protein, neutrophils, and lympholytes (all P<0.001) in BALF, and which also decreased the levels of TNF-α, IL-1β, TLR4 mRNA, and NF-κB p65 mRNA (all P<0.001) in the lungs of CLP-induced rats. Moreover, pretreatment of SNG significantly increased the levels of anti-oxidant enzymes GSH, SOD, GSH-Px, and catalase (all P<0.001) in the lung tissue of septic rats. Conclusion: SNG pretreatment has a protective effect on ALI in septic rats, and the specific mechanism may be related to anti-endotoxic, anti-inflammatory, and anti-oxidative properties. UR - https://ijbms.mums.ac.ir/article_15704.html L1 - https://ijbms.mums.ac.ir/article_15704_7762cc8b41537d760ea9ac9c39e0f8b4.pdf ER -