TY - JOUR ID - 15844 TI - TGF-β1 enhanced myocardial differentiation through inhibition of the Wnt/β-catenin pathway with rat BMSCs JO - Iranian Journal of Basic Medical Sciences JA - IJBMS LA - en SN - 2008-3866 AU - Lv, Yang AU - LI, Xiu-juan AU - Wang, Hai-Ping AU - Liu, Bo AU - Chen, Wei AU - Zhang, Lei AD - Department of Histology and Embryology, Hebei North University, Zhangjiakou, Hebei, China AD - Department of Pathology, the First Affiliated Hospital of Hebei North University, Zhangjiakou, Hebei, China AD - Department of Histology and Embryology, Hebei Medical University, Shijiazhuang, Hebei, China Y1 - 2020 PY - 2020 VL - 23 IS - 8 SP - 1012 EP - 1019 KW - Bone marrow mesenchymal KW - stem cells Cardiomyocytes Cell differentiation Transduction Transforming growth factor KW - beta1 Wnt signaling pathway DO - 10.22038/ijbms.2020.42396.10019 N2 - Objective(s): To investigate and test the hypotheses that TGF-β1 enhanced myocardial differentiation through Wnt/β-catenin pathway with rat bone marrow mesenchymal stem cells (BMSCs).Materials and Methods: Lentiviral vectors carrying the TGF-β1 gene were transduced into rat BMSCs firstly. Then several kinds of experimental methods were used to elucidate the related mechanisms by which TGF-β1 adjusts myocardial differentiation in rat BMSCs. Results: Immunocytochemistry revealed that cTnI and Cx43 expressed positively in the cells that were transduced with TGF-β1. The results of Western blot (WB) test showed that the levels of intranuclear β-catenin and total β-catenin were all significantly decreased. However, the cytoplasmic β-catenin level was largely unchanged. Moreover, the levels of GSK-3β were largely unchanged in BMSCs, whereas phosphorylated GSK-3β was significantly decreased in BMSCs. When given the activator of Wnt/β-catenin pathway (lithium chloride, LiCl) to BMSCs transducted with TGF-β1, β-catenin was increased, while phosphorylated β-catenin was decreased. In addition, cyclinD1, MMP-7, and c-Myc protein in BMSCs transducted with Lenti-TGF-β1-GFP were significantly lower. Conclusion: These results indicate that TGF-β1 promotes BMSCs cardiomyogenic differentiation by promoting the phosphorylation of β-catenin and inhibiting cyclinD1, MMP-7, and c-Myc expression in Wnt/β-catenin signaling pathway. UR - https://ijbms.mums.ac.ir/article_15844.html L1 - https://ijbms.mums.ac.ir/article_15844_9fab50176ed4d09b54759c46fd7e0c96.pdf ER -